################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BalI BamHI BanII BbrPI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiHKAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI DraI DraII Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MluNI MroI MscI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 299 GGCGCC G[GA]^CG[CT]C AflIII 1583 ACGCGT A^C[GA][CT]GT AgeI 1272 ACCGGT A^CCGGT AvaI 1254 CCCGAG C^[CT]CG[GA]G AviII 1566 TGCGCA TGC^GCA BbeI 299 GGCGCC GGCGC^C BcnI 1657 CCGGG CC^[GC]GG BglII 1356 AGATCT A^GATCT BsaHI 299 GGCGCC G[GA]^CG[CT]C BsgI 220 CTGCAC GTGCAG(16/14) BsiEI 1820 CGGCCG CG[GA][CT]^CG BsmBI 1763 CGTCTC CGTCTC(1/5) BsmI 316 GCATTC GAATGC(1/-1) BsoBI 1254 CCCGAG C^[CT]CG[GA]G BsrBI 1031 CCGCTC CCGCTC(-3/-3) BsrDI 1373 GCAATG GCAATG(2/0) BstAPI 222 GCACGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1356 AGATCT [GA]^GATC[CT] CpoI 1364 CGGACCG CG^G[AT]CCG DraIII 223 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1271 GACCGGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1820 CGGCCG C^GGCCG EclXI 1820 CGGCCG C^GGCCG Eco52I 1820 CGGCCG C^GGCCG EcoRV 1513 GATATC GAT^ATC FspI 1566 TGCGCA TGC^GCA HaeII 299 GGCGCC [GA]GCGC^[CT] Hin1I 299 GGCGCC G[GA]^CG[CT]C KasI 299 GGCGCC G^GCGCC MaeII 908 ACGT A^CGT MfeI 689 CAATTG C^AATTG MflI 1356 AGATCT [GA]^GATC[CT] MluI 1583 ACGCGT A^CGCGT MunI 689 CAATTG C^AATTG NaeI 1324 GCCGGC GCC^GGC NarI 299 GGCGCC GG^CGCC NciI 1657 CCGGG CC^[GC]GG NcoI 1664 CCATGG C^CATGG NdeI 830 CATATG CA^TATG NgoMIV 1324 GCCGGC G^CCGGC PinAI 1272 ACCGGT A^CCGGT PstI 605 CTGCAG CTGCA^G PvuII 1739 CAGCTG CAG^CTG RsrII 1364 CGGACCG CG^G[AT]CCG SfoI 299 GGCGCC GGC^GCC SgrAI 301 CGCCGGTG C[GA]^CCGG[CT]G SspI 822 AATATT AAT^ATT XcmI 587 CCAACGGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1356 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 1584 CGCG CG^CG 1849 CGCG Asp700I 409 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1108 GAAATGCTTC BbsI 426 GAAGAC GAAGAC(2/6) 252 GTCTTC BbvCI 1700 CCTCAGC CCTCAGC(-5/-2) 1854 GCTGAGG BmyI 229 GTGCCC G[AGT]GC[ACT]^C 1708 GTGCCC BpmI 206 CTCCAG CTGGAG(16/14) 761 CTCCAG BpuAI 426 GAAGAC GAAGAC(2/6) 252 GTCTTC BsaI 1269 GAGACC GGTCTC(1/5) 1870 GAGACC BsaWI 981 ACCGGA [AT]^CCGG[AT] 1272 ACCGGT Bsp1286I 229 GTGCCC G[AGT]GC[ACT]^C 1708 GTGCCC BstUI 1584 CGCG CG^CG 1849 CGCG EarI 1537 GAAGAG CTCTTC(1/4) 1927 GAAGAG EcoT14I 682 CCAAGG C^C[AT][AT]GG 1664 CCATGG HgaI 1582 GACGC GACGC(5/10) 1585 GCGTC Ksp632I 1537 GAAGAG CTCTTC(1/4) 1927 GAAGAG MslI 40 CACGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 372 CATAGAGGTG MvnI 1584 CGCG CG^CG 1849 CGCG PshAI 680 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1839 GACACAGGTC SmlI 349 CTTGAG C^T[CT][GA]AG 561 CTCAAG StyI 682 CCAAGG C^C[AT][AT]GG 1664 CCATGG XmnI 409 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1108 GAAATGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AluI AvaII BanI BbvI BsaJI BseRI BsiYI BslI BsmAI BsmFI BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I