(Circular) (Six-base) MAPSORT of: pFN21AE1466.seq Check: 6632 from: 1 to: 6700 pFN21AE1466 6700 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3668 3668 Size: 6700 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4510 278 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4043 1937 Size: 2106 4594 Acc65I G'GTAC_C Cuts at: 4021 4021 Size: 6700 AclI AA'CG_TT Cuts at: 1563 4827 5200 1563 Size: 3264 373 3063 Fragments arranged by size: 3264 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 4763 6147 6415 1632 Size: 972 2159 1384 268 1917 Fragments arranged by size: 2159 1917 1384 972 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6503 AflIII A'CryG_T Cuts at: 1203 3588 5599 6576 1203 Size: 2385 2011 977 1327 Fragments arranged by size: 2385 2011 1327 977 AgeI A'CCGG_T Cuts at: 3277 5531 3277 Size: 2254 4446 AhdI GACnn_n'nnGTC Cuts at: 5429 5429 Size: 6700 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6700 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6700 AlwNI CAG_nnn'CTG Cuts at: 4443 6015 4443 Size: 1572 5128 ApaLI G'TGCA_C Cuts at: 4757 5913 4757 Size: 1156 5544 ApoI r'AATT_y Cuts at: 1493 4009 4235 4387 6496 1493 Size: 2516 226 152 2109 1697 Fragments arranged by size: 2516 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5254 160 Size: 872 4222 1606 Fragments arranged by size: 4222 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 AvaI C'yCGr_G Cuts at: 1870 1943 3259 4025 1870 Size: 73 1316 766 4545 Fragments arranged by size: 4545 1316 766 73 BamHI G'GATC_C Cuts at: 4030 4397 4030 Size: 367 6333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2304 2310 2588 3062 3712 Size: 325 217 627 517 6 278 474 650 Cuts at: 3712 4021 5476 618 Size: 309 1455 1842 Fragments arranged by size: 1842 1455 650 627 517 474 325 309 278 217 6 BanII G_rGCy'C Cuts at: 1391 4019 1391 Size: 2628 4072 BbeI G_GCGC'C Cuts at: 2308 2308 Size: 6700 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 2438 928 Size: 971 351 188 5190 Fragments arranged by size: 5190 971 351 188 BbvCI CC'TCA_GC Cuts at: 3706 3860 3706 Size: 154 6546 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4891 4925 1903 Size: 34 2954 34 3678 Fragments arranged by size: 3678 2954 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4592 5802 4592 Size: 1210 5490 BclI T'GATC_A Cuts at: 1548 1548 Size: 6700 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5311 136 Size: 107 122 71 631 605 3639 1525 Fragments arranged by size: 3639 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3361 6695 3361 Size: 3334 3366 BlpI GC'TnA_GC Cuts at: 4117 4117 Size: 6700 Bme1580I G_kGCm'C Cuts at: 1790 2238 3717 4761 5917 1790 Size: 448 1479 1044 1156 2573 Fragments arranged by size: 2573 1479 1156 1044 448 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2232 3478 4431 Size: 416 53 83 399 24 815 1246 953 Cuts at: 4431 5389 442 Size: 958 1753 Fragments arranged by size: 1753 1246 958 953 815 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6700 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3492 3492 Size: 6700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2196 2751 5360 1239 Size: 957 555 2609 2579 Fragments arranged by size: 2609 2579 957 555 Bpu10I CC'TnA_GC Cuts at: 3706 3839 3860 3706 Size: 133 21 6546 Fragments arranged by size: 6546 133 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2375 2551 4825 5690 5988 2375 Size: 176 2274 865 298 3087 Fragments arranged by size: 3087 2274 865 298 176 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3268 3869 5363 882 Size: 617 1769 601 1494 2219 Fragments arranged by size: 2219 1769 1494 617 601 BsaAI yAC'GTr Cuts at: 493 1204 4491 493 Size: 711 3287 2702 Fragments arranged by size: 3287 2702 711 BsaBI GATnn'nnATC Cuts at: 6694 6694 Size: 6700 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2305 4507 4889 5524 Size: 53 83 186 936 772 2202 382 635 Cuts at: 5524 275 Size: 1451 Fragments arranged by size: 2202 1451 936 772 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2986 3277 5133 5531 5805 5952 6363 2986 Size: 291 1856 398 274 147 411 3323 Fragments arranged by size: 3323 1856 411 398 291 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2749 2779 2868 2898 1178 Size: 30 165 30 1346 30 89 30 4980 Fragments arranged by size: 4980 1346 165 89 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3594 3848 3877 1186 Size: 2408 254 29 4009 Fragments arranged by size: 4009 2408 254 29 BseYI C'CCAG_C Cuts at: 2040 5903 6399 2040 Size: 3863 496 2341 Fragments arranged by size: 3863 2341 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 1163 Size: 573 474 5653 Fragments arranged by size: 5653 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3828 4150 4911 5939 1540 Size: 275 186 1827 322 761 1028 2301 Fragments arranged by size: 2301 1827 1028 761 322 275 186 BsiHKAI G_wGCw'C Cuts at: 4761 4846 5917 4761 Size: 85 1071 5544 Fragments arranged by size: 5544 1071 85 BsmI GAATG_Cn' Cuts at: 2321 4211 4304 2321 Size: 1890 93 4717 Fragments arranged by size: 4717 1890 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3774 3774 Size: 6700 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 3717 4019 4761 4846 5917 1391 Size: 399 448 1479 302 742 85 1071 2174 Fragments arranged by size: 2174 1479 1071 742 448 399 302 85 BspEI T'CCGG_A Cuts at: 6363 6363 Size: 6700 BspHI T'CATG_A Cuts at: 4589 6319 4589 Size: 1730 4970 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 2758 3668 4055 844 Size: 1496 418 910 387 3489 Fragments arranged by size: 3489 1496 910 418 387 BsrBI CCG'CTC Cuts at: 1394 1823 3038 4587 1394 Size: 429 1215 1549 3507 Fragments arranged by size: 3507 1549 1215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3385 5195 5369 65 Size: 1183 2137 1810 174 1396 Fragments arranged by size: 2137 1810 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2307 2719 3277 3329 5344 5531 1700 Size: 110 497 412 558 52 2015 187 2869 Fragments arranged by size: 2869 2015 558 497 412 187 110 52 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6700 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6700 BssSI C'ACGA_G Cuts at: 4760 5772 4760 Size: 1012 5688 BstAPI GCAn_nnn'nTGC Cuts at: 794 2233 794 Size: 1439 5261 BstBI TT'CG_AA Cuts at: 5504 5504 Size: 6700 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6700 BstYI r'GATC_y Cuts at: 1972 3361 4030 4397 4782 4799 6251 6337 6349 Size: 1389 669 367 385 17 1452 86 12 Cuts at: 6349 6695 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1389 669 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3408 3669 3828 513 Size: 552 939 1404 261 159 3385 Fragments arranged by size: 3385 1404 939 552 261 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5961 Fragments arranged by size: 5961 683 56 BtsI GCAGTG_nn' Cuts at: 3251 4216 5010 5030 3251 Size: 965 794 20 4921 Fragments arranged by size: 4921 965 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6700 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6665 DraI TTT'AAA Cuts at: 4004 4004 Size: 6700 DraIII CAC_nnn'GTG Cuts at: 2233 2233 Size: 6700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3282 5707 809 Size: 507 1966 2425 1802 Fragments arranged by size: 2425 1966 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3215 3327 3810 Size: 54 1425 50 275 18 1385 112 483 Cuts at: 3810 3825 4147 5036 6665 8 Size: 15 322 889 1629 43 Fragments arranged by size: 1629 1425 1385 889 483 322 275 112 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3825 4147 1537 Size: 275 2013 322 4090 Fragments arranged by size: 4090 2013 322 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3537 3927 4630 1353 Size: 2184 390 703 3423 Fragments arranged by size: 3423 2184 703 390 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5287 5661 5807 387 Size: 1064 138 3698 374 146 1280 Fragments arranged by size: 3698 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2604 2751 4763 5360 6147 6415 Size: 393 564 408 147 2012 597 787 268 Cuts at: 6415 1239 Size: 1524 Fragments arranged by size: 2012 1524 787 597 564 408 393 268 147 EcoRI G'AATT_C Cuts at: 4009 4387 4009 Size: 378 6322 EcoRV GAT'ATC Cuts at: 3520 3520 Size: 6700 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3340 3340 Size: 6700 FspI TGC'GCA Cuts at: 3573 5206 3573 Size: 1633 5067 HaeII r_GCGC'y Cuts at: 1678 2308 5847 1678 Size: 630 3539 2531 Fragments arranged by size: 3539 2531 630 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 2868 2900 3217 Size: 32 135 32 926 32 338 32 317 Cuts at: 3217 3249 5341 5373 5415 5447 1373 Size: 32 2092 32 42 32 2626 Fragments arranged by size: 2626 2092 926 338 317 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4044 4290 669 Size: 988 281 2106 246 3079 Fragments arranged by size: 3079 2106 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6700 HpaI GTT'AAC Cuts at: 4290 4290 Size: 6700 KasI G'GCGC_C Cuts at: 2304 2304 Size: 6700 KpnI G_GTAC'C Cuts at: 4025 4025 Size: 6700 MfeI C'AATT_G Cuts at: 2694 4299 2694 Size: 1605 5095 MluI A'CGCG_T Cuts at: 3588 6576 3588 Size: 2988 3712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 2475 3464 3906 5814 5998 1305 Size: 1155 15 989 442 1908 184 2007 Fragments arranged by size: 2007 1908 1155 989 442 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4878 Fragments arranged by size: 4878 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2049 2381 4658 5017 5176 518 Size: 648 883 332 2277 359 159 2042 Fragments arranged by size: 2277 2042 883 648 359 332 159 MspA1I CmG'CkG Cuts at: 1606 3289 3710 3746 4116 4793 5941 6186 1606 Size: 1683 421 36 370 677 1148 245 2120 Fragments arranged by size: 2120 1683 1148 677 421 370 245 36 NaeI GCC'GGC Cuts at: 3331 3331 Size: 6700 NarI GG'CG_CC Cuts at: 2305 2305 Size: 6700 NcoI C'CATG_G Cuts at: 513 1065 2004 3669 513 Size: 552 939 1665 3544 Fragments arranged by size: 3544 1665 939 552 NdeI CA'TA_TG Cuts at: 387 2836 387 Size: 2449 4251 NgoMIV G'CCGG_C Cuts at: 3329 3329 Size: 6700 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6700 NotI GC'GGCC_GC Cuts at: 4147 4147 Size: 6700 NspI r_CATG'y Cuts at: 1132 1229 4058 5603 1132 Size: 97 2829 1545 2229 Fragments arranged by size: 2829 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6700 PciI A'CATG_T Cuts at: 5599 5599 Size: 6700 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6700 PfoI T'CCnGG_A Cuts at: 3351 3351 Size: 6700 PmeI GTTT'AAAC Cuts at: 4004 4004 Size: 6700 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4769 4801 6282 6314 4769 Size: 32 1481 32 5155 Fragments arranged by size: 5155 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2689 3848 1613 Size: 1076 1159 4465 Fragments arranged by size: 4465 1159 1076 PsiI TTA'TAA Cuts at: 4270 4270 Size: 6700 PstI C_TGCA'G Cuts at: 830 2614 4052 830 Size: 1784 1438 3478 Fragments arranged by size: 3478 1784 1438 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6700 PvuII CAG'CTG Cuts at: 3746 3746 Size: 6700 RsrII CG'GwC_CG Cuts at: 3370 3370 Size: 6700 SalI G'TCGA_C Cuts at: 1936 4042 1936 Size: 2106 4594 SbfI CC_TGCA'GG Cuts at: 4052 4052 Size: 6700 ScaI AGT'ACT Cuts at: 4948 6660 4948 Size: 1712 4988 SfcI C'TryA_G Cuts at: 826 1045 2610 2757 3462 4048 5183 5864 6055 Size: 219 1565 147 705 586 1135 681 191 Cuts at: 6055 826 Size: 1471 Fragments arranged by size: 1565 1471 1135 705 681 586 219 191 147 SfoI GGC'GCC Cuts at: 2306 2306 Size: 6700 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 SgrAI Cr'CCGG_yG Cuts at: 1700 2307 1700 Size: 607 6093 SmaI CCC'GGG Cuts at: 1872 4027 1872 Size: 2155 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2354 2566 4804 5705 5967 820 Size: 197 926 411 212 2238 901 262 1553 Fragments arranged by size: 2238 1553 926 901 411 262 212 197 SnaBI TAC'GTA Cuts at: 493 4491 493 Size: 3998 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6700 SphI G_CATG'C Cuts at: 1132 4058 1132 Size: 2926 3774 SspI AAT'ATT Cuts at: 5 52 2829 4624 5 Size: 47 2777 1795 2081 Fragments arranged by size: 2777 2081 1795 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6700 StyI C'CwwG_G Cuts at: 513 1065 2004 2687 3669 4139 513 Size: 552 939 683 982 470 3074 Fragments arranged by size: 3074 982 939 683 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 4727 4744 4897 5082 5527 325 Size: 1255 480 2667 17 153 185 445 1498 Fragments arranged by size: 2667 1498 1255 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4946 6658 96 Size: 274 80 33 51 1443 2969 1712 138 Fragments arranged by size: 2969 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2425 6660 6692 2393 Size: 32 4235 32 2401 Fragments arranged by size: 4235 2401 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6700 XbaI T'CTAG_A Cuts at: 4036 4036 Size: 6700 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2599 2599 Size: 6700 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6700 XmaI C'CCGG_G Cuts at: 1870 4025 1870 Size: 2155 4545 XmnI GAAnn'nnTTC Cuts at: 2418 3117 4829 2418 Size: 699 1712 4289 Fragments arranged by size: 4289 1712 699 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4508 276 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SrfI SwaI