Restriction map for pFN21AE1466
 (Linear) (Six-base) MAPSORT of: KIEE1466.seq  Check: 7994  from: 1  to: 1995



KIEE1466.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1663    1995 
   Size:       1663     332
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     599    1995 
   Size:        599    1396
 
AflIII A'CryG_T

Cuts at:      0    1583    1995 
   Size:       1583     412
 
AgeI A'CCGG_T

Cuts at:      0    1272    1995 
   Size:       1272     723
 
AvaI C'yCGr_G

Cuts at:      0    1254    1995 
   Size:       1254     741
 
BanI G'GyrC_C

Cuts at:      0     299     305     583    1057    1707    1995 
   Size:        299       6     278     474     650     288

  Fragments arranged by size: 

                 650     474     299     288     278       6
 
BbeI G_GCGC'C

Cuts at:      0     303    1995 
   Size:        303    1692
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     245     433    1995 
   Size:        245     188    1562

  Fragments arranged by size: 

                1562     245     188
 
BbvCI CC'TCA_GC

Cuts at:      0    1701    1855    1995 
   Size:       1701     154     140

  Fragments arranged by size: 

                1701     154     140
 
BglII A'GATC_T

Cuts at:      0    1356    1995 
   Size:       1356     639
 
Bme1580I G_kGCm'C

Cuts at:      0     233    1712    1995 
   Size:        233    1479     283

  Fragments arranged by size: 

                1479     283     233
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     227    1473    1995 
   Size:        227    1246     522

  Fragments arranged by size: 

                1246     522     227
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1487    1995 
   Size:       1487     508
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     746    1995 
   Size:        191     555    1249

  Fragments arranged by size: 

                1249     555     191
 
Bpu10I CC'TnA_GC

Cuts at:      0    1701    1834    1855    1995 
   Size:       1701     133      21     140

  Fragments arranged by size: 

                1701     140     133      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     546    1995 
   Size:        370     176    1449

  Fragments arranged by size: 

                1449     370     176
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1263    1864    1995 
   Size:       1263     601     131

  Fragments arranged by size: 

                1263     601     131
 
BsaHI Gr'CG_yC

Cuts at:      0     300    1995 
   Size:        300    1695
 
BsaWI w'CCGG_w

Cuts at:      0     981    1272    1995 
   Size:        981     291     723

  Fragments arranged by size: 

                 981     723     291
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     744     774     863     893    1995 
   Size:        744      30      89      30    1102

  Fragments arranged by size: 

                1102     744      89      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1589    1843    1872    1995 
   Size:       1589     254      29     123

  Fragments arranged by size: 

                1589     254     123      29
 
BseYI C'CCAG_C

Cuts at:      0      35    1995 
   Size:         35    1960
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205    1995 
   Size:        205    1790
 
BsiEI CG_ry'CG

Cuts at:      0    1823    1995 
   Size:       1823     172
 
BsmI GAATG_Cn'

Cuts at:      0     316    1995 
   Size:        316    1679
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1769    1995 
   Size:       1769     226
 
Bsp1286I G_dGCh'C

Cuts at:      0     233    1712    1995 
   Size:        233    1479     283

  Fragments arranged by size: 

                1479     283     233
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     335     753    1663    1995 
   Size:        335     418     910     332

  Fragments arranged by size: 

                 910     418     335     332
 
BsrBI CCG'CTC

Cuts at:      0    1033    1995 
   Size:       1033     962
 
BsrDI GCAATG_nn'

Cuts at:      0    1380    1995 
   Size:       1380     615
 
BsrFI r'CCGG_y

Cuts at:      0     302     714    1272    1324    1995 
   Size:        302     412     558      52     671

  Fragments arranged by size: 

                 671     558     412     302      52
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     228    1995 
   Size:        228    1767
 
BstYI r'GATC_y

Cuts at:      0    1356    1995 
   Size:       1356     639
 
BtgI C'CryG_G

Cuts at:      0    1403    1664    1823    1995 
   Size:       1403     261     159     172

  Fragments arranged by size: 

                1403     261     172     159
 
BtsI GCAGTG_nn'

Cuts at:      0    1246    1995 
   Size:       1246     749
 
DraIII CAC_nnn'GTG

Cuts at:      0     228    1995 
   Size:        228    1767
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1277    1995 
   Size:       1277     718
 
EaeI y'GGCC_r

Cuts at:      0    1210    1322    1805    1820    1995 
   Size:       1210     112     483      15     175

  Fragments arranged by size: 

                1210     483     175     112      15
 
EagI C'GGCC_G

Cuts at:      0    1820    1995 
   Size:       1820     175
 
EarI CTCTTCn'nnn_

Cuts at:      0    1532    1922    1995 
   Size:       1532     390      73

  Fragments arranged by size: 

                1532     390      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     599     746    1995 
   Size:        191     408     147    1249

  Fragments arranged by size: 

                1249     408     191     147
 
EcoRV GAT'ATC

Cuts at:      0    1515    1995 
   Size:       1515     480
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1335    1995 
   Size:       1335     660
 
FspI TGC'GCA

Cuts at:      0    1568    1995 
   Size:       1568     427
 
HaeII r_GCGC'y

Cuts at:      0     303    1995 
   Size:        303    1692
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     493     525     863     895    1212    1244    1995 
   Size:        493      32     338      32     317      32     751

  Fragments arranged by size: 

                 751     493     338     317      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     299    1995 
   Size:        299    1696
 
MfeI C'AATT_G

Cuts at:      0     689    1995 
   Size:        689    1306
 
MluI A'CGCG_T

Cuts at:      0    1583    1995 
   Size:       1583     412
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     470    1459    1901    1995 
   Size:        455      15     989     442      94

  Fragments arranged by size: 

                 989     455     442      94      15
 
MslI CAynn'nnrTG

Cuts at:      0      44     376    1995 
   Size:         44     332    1619

  Fragments arranged by size: 

                1619     332      44
 
MspA1I CmG'CkG

Cuts at:      0    1284    1705    1741    1995 
   Size:       1284     421      36     254

  Fragments arranged by size: 

                1284     421     254      36
 
NaeI GCC'GGC

Cuts at:      0    1326    1995 
   Size:       1326     669
 
NarI GG'CG_CC

Cuts at:      0     300    1995 
   Size:        300    1695
 
NcoI C'CATG_G

Cuts at:      0    1664    1995 
   Size:       1664     331
 
NdeI CA'TA_TG

Cuts at:      0     831    1995 
   Size:        831    1164
 
NgoMIV G'CCGG_C

Cuts at:      0    1324    1995 
   Size:       1324     671
 
PfoI T'CCnGG_A

Cuts at:      0    1346    1995 
   Size:       1346     649
 
PshAI GACnn'nnGTC

Cuts at:      0     684    1843    1995 
   Size:        684    1159     152

  Fragments arranged by size: 

                1159     684     152
 
PstI C_TGCA'G

Cuts at:      0     609    1995 
   Size:        609    1386
 
PvuII CAG'CTG

Cuts at:      0    1741    1995 
   Size:       1741     254
 
RsrII CG'GwC_CG

Cuts at:      0    1365    1995 
   Size:       1365     630
 
SfcI C'TryA_G

Cuts at:      0     605     752    1457    1995 
   Size:        605     147     705     538

  Fragments arranged by size: 

                 705     605     538     147
 
SfoI GGC'GCC

Cuts at:      0     301    1995 
   Size:        301    1694
 
SgrAI Cr'CCGG_yG

Cuts at:      0     302    1995 
   Size:        302    1693
 
SmlI C'TyrA_G

Cuts at:      0     349     561    1995 
   Size:        349     212    1434

  Fragments arranged by size: 

                1434     349     212
 
SspI AAT'ATT

Cuts at:      0     824    1995 
   Size:        824    1171
 
StyI C'CwwG_G

Cuts at:      0     682    1664    1995 
   Size:        682     982     331

  Fragments arranged by size: 

                 982     682     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55    1995 
   Size:         55    1940
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     388     420    1995 
   Size:        388      32    1575

  Fragments arranged by size: 

                1575     388      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     594    1995 
   Size:        594    1401
 
XmnI GAAnn'nnTTC

Cuts at:      0     413    1112    1995 
   Size:        413     699     883

  Fragments arranged by size: 

                 883     699     413
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AvaI     BanI     BbeI     BbsI
    BbvCI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI     EagI     EarI
  Eco57MI    EcoRV     FalI     FspI    HaeII    Hin4I     KasI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     PfoI    PshAI     PstI    PvuII    RsrII     SfcI     SfoI
    SgrAI     SmlI     SspI     StyI    TaqII     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI    BmgBI     BmtI    BsaAI    BsaBI  BsiHKAI
    BsiWI    BspEI    BspHI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MscI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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