################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AviII AvrII BbvCI BcgI BciVI BfrI BlnI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI SacII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 2371 GAATTT [GA]^AATT[CT] AocI 1789 CCTCAGG CC^T[ACGT]AGG ApoI 2371 GAATTT [GA]^AATT[CT] Asp700I 2244 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 959 GGCGCC GGCGC^C BbrPI 1816 CACGTG CAC^GTG BclI 2055 TGATCA T^GATCA BfaI 728 CTAG C^TAG BsiEI 694 CGACCG CG[GA][CT]^CG BspLU11I 2636 ACATGT A^CATGT BsrBI 1492 GAGCGG CCGCTC(-3/-3) BssSI 1216 CACGAG CACGAG(-5/-1) Bsu36I 1789 CCTCAGG CC^T[ACGT]AGG CpoI 1495 CGGACCG CG^G[AT]CCG DraIII 1406 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 717 GACTTACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1789 CCTCAGG CC^T[ACGT]AGG FbaI 2055 TGATCA T^GATCA HgaI 311 GCGTC GACGC(5/10) KasI 959 GGCGCC G^GCGCC MaeI 728 CTAG C^TAG NarI 959 GGCGCC GG^CGCC PflFI 2479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2481 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1816 CACGTG CAC^GTG PmlI 1816 CACGTG CAC^GTG RsrII 1495 CGGACCG CG^G[AT]CCG SacI 1378 GAGCTC GAGCT^C SapI 2025 GAAGAGC GCTCTTC(1/4) SbfI 1593 CCTGCAGG CCTGCA^GG ScaI 2573 AGTACT AGT^ACT SfoI 959 GGCGCC GGC^GCC SmaI 2210 CCCGGG CCC^GGG SmlI 1300 CTCAAG C^T[CT][GA]AG Sse8387I 1593 CCTGCAGG CCTGCA^GG TfiI 13 GATTC G^A[AT]TC Tsp509I 2372 AATT ^AATT Tth111I 2479 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2481 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2210 CCCGGG C^CCGGG XmaI 2210 CCCGGG C^CCGGG XmnI 2244 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 134 TCCGGA T^CCGGA 1157 TCCGGA AcyI 310 GGCGTC G[GA]^CG[CT]C 959 GGCGCC AflIII 722 ACGTGT A^C[GA][CT]GT 2636 ACATGT AhdI 2440 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2632 GACTACATGTC Alw44I 259 GTGCAC G^TGCAC 1612 GTGCAC ApaI 1460 GGGCCC GGGCC^C 1927 GGGCCC ApaLI 259 GTGCAC G^TGCAC 1612 GTGCAC AspEI 2440 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2632 GACTACATGTC BamHI 866 GGATCC G^GATCC 1934 GGATCC BbsI 673 GAAGAC GAAGAC(2/6) 829 GAAGAC BglI 754 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1543 GCCAAGCGGGC BglII 266 AGATCT A^GATCT 1433 AGATCT BlpI 1038 GCTCAGC GC^T[ACGT]AGC 2001 GCTCAGC Bpu1102I 1038 GCTCAGC GC^T[ACGT]AGC 2001 GCTCAGC BpuAI 673 GAAGAC GAAGAC(2/6) 829 GAAGAC BsaAI 721 TACGTG [CT]AC^GT[GA] 1816 CACGTG BsaHI 310 GGCGTC G[GA]^CG[CT]C 959 GGCGCC BseAI 134 TCCGGA T^CCGGA 1157 TCCGGA BspEI 134 TCCGGA T^CCGGA 1157 TCCGGA BspHI 713 TCATGA T^CATGA 2384 TCATGA BstXI 656 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2402 CCAACCGCCTGG CelII 1038 GCTCAGC GC^T[ACGT]AGC 2001 GCTCAGC Eam1105I 2440 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2632 GACTACATGTC EcoNI 132 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1110 CCTGGAGCAGG EcoT22I 916 ATGCAT ATGCA^T 1978 ATGCAT Hin1I 310 GGCGTC G[GA]^CG[CT]C 959 GGCGCC MroI 134 TCCGGA T^CCGGA 1157 TCCGGA NsiI 916 ATGCAT ATGCA^T 1978 ATGCAT PleI 1402 GACTC GAGTC(4/5) 2611 GACTC PstI 1021 CTGCAG CTGCA^G 1594 CTGCAG RcaI 713 TCATGA T^CATGA 2384 TCATGA SfcI 1021 CTGCAG C^T[GA][CT]AG 1594 CTGCAG SphI 918 GCATGC GCATG^C 1863 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII