(Circular) (Six-base) MAPSORT of: pFN21AE2247.seq Check: 1024 from: 1 to: 7381 pFN21AE2247 7381 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3112 4023 3112 Size: 911 6470 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5191 278 Size: 53 83 186 936 3655 2468 Fragments arranged by size: 3655 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4724 1937 Size: 2787 4594 Acc65I G'GTAC_C Cuts at: 4702 4702 Size: 7381 AclI AA'CG_TT Cuts at: 1563 5508 5881 1563 Size: 3945 373 3063 Fragments arranged by size: 3945 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2421 5444 6828 7096 1632 Size: 581 208 3023 1384 268 1917 Fragments arranged by size: 3023 1917 1384 581 268 208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7184 AflIII A'CryG_T Cuts at: 1203 2727 4641 6280 7257 1203 Size: 1524 1914 1639 977 1327 Fragments arranged by size: 1914 1639 1524 1327 977 AgeI A'CCGG_T Cuts at: 6212 6212 Size: 7381 AhdI GACnn_n'nnGTC Cuts at: 4450 4642 6110 4450 Size: 192 1468 5721 Fragments arranged by size: 5721 1468 192 AleI CACnn'nnGTG Cuts at: 1166 4143 1166 Size: 2977 4404 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7381 AlwNI CAG_nnn'CTG Cuts at: 2941 3643 4210 5124 6696 2941 Size: 702 567 914 1572 3626 Fragments arranged by size: 3626 1572 914 702 567 ApaI G_GGCC'C Cuts at: 3469 3936 3469 Size: 467 6914 ApaLI G'TGCA_C Cuts at: 2264 3617 5438 6594 2264 Size: 1353 1821 1156 3051 Fragments arranged by size: 3051 1821 1353 1156 ApoI r'AATT_y Cuts at: 1493 4376 4690 4916 5068 7177 1493 Size: 2883 314 226 152 2109 1697 Fragments arranged by size: 2883 2109 1697 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 5935 160 Size: 872 4903 1606 Fragments arranged by size: 4903 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7381 AvaI C'yCGr_G Cuts at: 1870 1943 2567 2909 4215 4307 4706 1870 Size: 73 624 342 1306 92 399 4545 Fragments arranged by size: 4545 1306 624 399 342 92 73 BamHI G'GATC_C Cuts at: 2871 3939 4711 5078 2871 Size: 1068 772 367 5174 Fragments arranged by size: 5174 1068 772 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2905 2964 3767 3835 4702 Size: 325 217 627 1118 59 803 68 867 Cuts at: 4702 6157 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1118 867 803 627 325 217 68 59 BanII G_rGCy'C Cuts at: 1391 2761 3387 3469 3916 3936 4700 1391 Size: 1370 626 82 447 20 764 4072 Fragments arranged by size: 4072 1370 764 626 447 82 20 BbeI G_GCGC'C Cuts at: 2968 2968 Size: 7381 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2685 2841 928 Size: 971 786 156 5468 Fragments arranged by size: 5468 971 786 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5572 5606 1903 Size: 34 3635 34 3678 Fragments arranged by size: 3678 3635 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5273 6483 5273 Size: 1210 6171 BclI T'GATC_A Cuts at: 1548 4060 1548 Size: 2512 4869 BfrBI ATG'CAT Cuts at: 2923 3985 2923 Size: 1062 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2765 3554 5992 Size: 107 122 71 631 605 1093 789 2438 Cuts at: 5992 136 Size: 1525 Fragments arranged by size: 2438 1525 1093 789 631 605 122 107 71 BglII A'GATC_T Cuts at: 2271 3438 7376 2271 Size: 1167 3938 2276 Fragments arranged by size: 3938 2276 1167 BlpI GC'TnA_GC Cuts at: 3044 4007 4798 3044 Size: 963 791 5627 Fragments arranged by size: 5627 963 791 Bme1580I G_kGCm'C Cuts at: 1790 2268 2910 3469 3621 3770 3936 5442 6598 Size: 478 642 559 152 149 166 1506 1156 Cuts at: 6598 1790 Size: 2573 Fragments arranged by size: 2573 1506 1156 642 559 478 166 152 149 BmgBI CAC'GTC Cuts at: 1318 2635 3754 1318 Size: 1317 1119 4945 Fragments arranged by size: 4945 1317 1119 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2068 3408 4310 Size: 416 53 83 399 24 651 1340 902 Cuts at: 4310 5112 6070 442 Size: 802 958 1753 Fragments arranged by size: 1753 1340 958 902 802 651 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7381 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 7381 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2775 2915 2963 3026 3137 3191 3515 Size: 992 544 140 48 63 111 54 324 Cuts at: 3515 3536 4016 4088 6041 1239 Size: 21 480 72 1953 2579 Fragments arranged by size: 2579 1953 992 544 480 324 140 111 72 63 54 48 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3290 5506 6371 6669 3290 Size: 2216 865 298 4002 Fragments arranged by size: 4002 2216 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3091 3266 3512 6044 882 Size: 617 1592 175 246 2532 2219 Fragments arranged by size: 2532 2219 1592 617 246 175 BsaAI yAC'GTr Cuts at: 493 1204 2728 3823 5172 493 Size: 711 1524 1095 1349 2702 Fragments arranged by size: 2702 1524 1349 1095 711 BsaBI GATnn'nnATC Cuts at: 7375 7375 Size: 7381 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2965 5188 5570 Size: 53 83 186 936 783 649 2223 382 Cuts at: 5570 6205 275 Size: 635 1451 Fragments arranged by size: 2223 1451 936 783 649 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 2701 3162 5814 6212 6486 6633 7044 2139 Size: 562 461 2652 398 274 147 411 2476 Fragments arranged by size: 2652 2476 562 461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 1178 Size: 30 165 30 855 30 6271 Fragments arranged by size: 6271 855 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3155 3644 3878 3971 4325 1186 Size: 1969 489 234 93 354 4242 Fragments arranged by size: 4242 1969 489 354 234 93 BseYI C'CCAG_C Cuts at: 4152 4514 6584 7080 4152 Size: 362 2070 496 4453 Fragments arranged by size: 4453 2070 496 362 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2954 3008 3543 1163 Size: 573 1218 54 535 5001 Fragments arranged by size: 5001 1218 573 535 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2702 4831 5592 6620 1540 Size: 275 186 701 2129 761 1028 2301 Fragments arranged by size: 2301 2129 1028 761 701 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 3387 3621 4140 4633 5442 5527 6598 2268 Size: 1119 234 519 493 809 85 1071 3051 Fragments arranged by size: 3051 1119 1071 809 519 493 234 85 BsmI GAATG_Cn' Cuts at: 4892 4985 4892 Size: 93 7288 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2761 2910 3387 3469 3621 3770 Size: 399 478 493 149 477 82 152 149 Cuts at: 3770 3916 3936 4140 4633 4700 5442 5527 6598 Size: 146 20 204 493 67 742 85 1071 Cuts at: 6598 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 493 493 478 477 399 204 152 149 149 146 85 82 67 20 BspEI T'CCGG_A Cuts at: 2139 3162 7044 2139 Size: 1023 3882 2476 Fragments arranged by size: 3882 2476 1023 BspHI T'CATG_A Cuts at: 2718 4389 5270 7000 2718 Size: 1671 881 1730 3099 Fragments arranged by size: 3099 1730 1671 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 3112 3606 4023 4736 844 Size: 1235 1033 494 417 713 3489 Fragments arranged by size: 3489 1235 1033 713 494 417 BsrBI CCG'CTC Cuts at: 1394 1823 3499 5268 1394 Size: 429 1676 1769 3507 Fragments arranged by size: 3507 1769 1676 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5876 6050 65 Size: 1183 4628 174 1396 Fragments arranged by size: 4628 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2562 2961 3051 4607 6025 6212 1700 Size: 110 752 399 90 1556 1418 187 2869 Fragments arranged by size: 2869 1556 1418 752 399 187 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7381 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7381 BssSI C'ACGA_G Cuts at: 3221 5441 6453 3221 Size: 2220 1012 4149 Fragments arranged by size: 4149 2220 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2929 3835 4379 794 Size: 2135 906 544 3796 Fragments arranged by size: 3796 2135 906 544 BstBI TT'CG_AA Cuts at: 6185 6185 Size: 7381 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7381 BstXI CCAn_nnnn'nTGG Cuts at: 2668 4414 2668 Size: 1746 5635 BstYI r'GATC_y Cuts at: 1972 2271 2871 3159 3438 3633 3939 3969 4206 Size: 299 600 288 279 195 306 30 237 Cuts at: 4206 4506 4711 5078 5463 5480 6932 7018 7030 Size: 300 205 367 385 17 1452 86 12 Cuts at: 7030 7376 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 600 385 367 346 306 300 299 288 279 237 205 195 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3795 3795 Size: 7381 BtgI C'CryG_G Cuts at: 513 1065 2004 2424 2888 3588 4131 4536 4653 Size: 552 939 420 464 700 543 405 117 Cuts at: 4653 513 Size: 3241 Fragments arranged by size: 3241 939 700 552 543 464 420 405 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3358 4159 4570 487 Size: 683 56 2132 801 411 3298 Fragments arranged by size: 3298 2132 801 683 411 56 BtsI GCAGTG_nn' Cuts at: 4897 5691 5711 4897 Size: 794 20 6567 Fragments arranged by size: 6567 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7381 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7346 DraI TTT'AAA Cuts at: 4685 4685 Size: 7381 DraIII CAC_nnn'GTG Cuts at: 3416 3416 Size: 7381 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2728 6388 809 Size: 507 1412 3660 1802 Fragments arranged by size: 3660 1802 1412 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 2802 2850 Size: 54 1425 50 275 18 405 567 48 Cuts at: 2850 3702 4828 5717 7346 8 Size: 852 1126 889 1629 43 Fragments arranged by size: 1629 1425 1126 889 852 567 405 275 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4828 1537 Size: 275 3016 4090 Fragments arranged by size: 4090 3016 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 2166 2424 4025 5311 1353 Size: 693 120 258 1601 1286 3423 Fragments arranged by size: 3423 1601 1286 693 258 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2783 2864 5968 6342 6488 387 Size: 1064 138 1194 81 3104 374 146 1280 Fragments arranged by size: 3104 1280 1194 1064 374 146 138 81 EcoICRI GAG'CTC Cuts at: 3385 3385 Size: 7381 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2231 2421 2775 2915 2963 3026 Size: 393 581 18 190 354 140 48 63 Cuts at: 3026 3137 3191 3515 3536 4016 4088 5444 6041 Size: 111 54 324 21 480 72 1356 597 Cuts at: 6041 6828 7096 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1356 787 597 581 480 393 354 324 268 190 140 111 72 63 54 48 21 18 EcoNI CCTnn'n_nnAGG Cuts at: 2141 3119 2141 Size: 978 6403 EcoO109I rG'GnC_Cy Cuts at: 2068 2290 3298 3465 3466 3595 3933 3990 4304 Size: 222 1008 167 1 129 338 57 314 Cuts at: 4304 2068 Size: 5145 Fragments arranged by size: 5145 1008 338 314 222 167 129 57 1 EcoRI G'AATT_C Cuts at: 4690 5068 4690 Size: 378 7003 FspI TGC'GCA Cuts at: 5887 5887 Size: 7381 HaeII r_GCGC'y Cuts at: 1678 2968 3700 4100 4661 6528 1678 Size: 1290 732 400 561 1867 2531 Fragments arranged by size: 2531 1867 1290 732 561 400 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4436 4468 4628 4660 6022 Size: 32 135 32 2864 32 160 32 1362 Cuts at: 6022 6054 6096 6128 1373 Size: 32 42 32 2626 Fragments arranged by size: 2864 2626 1362 160 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2284 2497 2809 3421 4042 4725 Size: 988 281 346 213 312 612 621 683 Cuts at: 4725 4971 669 Size: 246 3079 Fragments arranged by size: 3079 988 683 621 612 346 312 281 246 213 HindIII A'AGCT_T Cuts at: 748 748 Size: 7381 HpaI GTT'AAC Cuts at: 4971 4971 Size: 7381 KasI G'GCGC_C Cuts at: 2964 2964 Size: 7381 KpnI G_GTAC'C Cuts at: 4706 4706 Size: 7381 MfeI C'AATT_G Cuts at: 4980 4980 Size: 7381 MluI A'CGCG_T Cuts at: 7257 7257 Size: 7381 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 2668 2844 6495 6679 1305 Size: 996 367 176 3651 184 2007 Fragments arranged by size: 3651 2007 996 367 184 176 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 2852 3704 10 Size: 54 1425 343 405 615 852 3687 Fragments arranged by size: 3687 1425 852 615 405 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 3980 4143 4145 4535 5339 5698 Size: 648 1257 1557 163 2 390 804 359 Cuts at: 5698 5857 518 Size: 159 2042 Fragments arranged by size: 2042 1557 1257 804 648 390 359 163 159 2 MspA1I CmG'CkG Cuts at: 1606 3004 3532 3832 4066 4162 4797 5474 6622 Size: 1398 528 300 234 96 635 677 1148 Cuts at: 6622 6867 1606 Size: 245 2120 Fragments arranged by size: 2120 1398 1148 677 635 528 300 245 234 96 NarI GG'CG_CC Cuts at: 2965 2965 Size: 7381 NcoI C'CATG_G Cuts at: 513 1065 2004 3588 4131 4536 513 Size: 552 939 1584 543 405 3358 Fragments arranged by size: 3358 1584 939 552 543 405 NdeI CA'TA_TG Cuts at: 387 387 Size: 7381 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7381 NotI GC'GGCC_GC Cuts at: 4828 4828 Size: 7381 NsiI A_TGCA'T Cuts at: 2925 3987 2925 Size: 1062 6319 NspI r_CATG'y Cuts at: 1132 1229 2044 2927 3872 4645 4739 6284 1132 Size: 97 815 883 945 773 94 1545 2229 Fragments arranged by size: 2229 1545 945 883 815 773 97 94 PasI CC'CwG_GG Cuts at: 1348 2374 4363 1348 Size: 1026 1989 4366 Fragments arranged by size: 4366 1989 1026 PciI A'CATG_T Cuts at: 4641 6280 4641 Size: 1639 5742 PflMI CCAn_nnn'nTGG Cuts at: 1108 4492 1108 Size: 3384 3997 PfoI T'CCnGG_A Cuts at: 2076 2181 2412 2952 4089 2076 Size: 105 231 540 1137 5368 Fragments arranged by size: 5368 1137 540 231 105 PmeI GTTT'AAAC Cuts at: 4685 4685 Size: 7381 PmlI CAC'GTG Cuts at: 3823 3823 Size: 7381 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5450 5482 6963 6995 5450 Size: 32 1481 32 5836 Fragments arranged by size: 5836 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 3595 3990 4304 2068 Size: 1527 395 314 5145 Fragments arranged by size: 5145 1527 395 314 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7381 PsiI TTA'TAA Cuts at: 4951 4951 Size: 7381 PspOMI G'GGCC_C Cuts at: 3465 3932 3465 Size: 467 6914 PstI C_TGCA'G Cuts at: 830 3030 3603 4733 830 Size: 2200 573 1130 3478 Fragments arranged by size: 3478 2200 1130 573 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7381 PvuII CAG'CTG Cuts at: 3004 3532 3832 4066 4162 3004 Size: 528 300 234 96 6223 Fragments arranged by size: 6223 528 300 234 96 RsrII CG'GwC_CG Cuts at: 3501 3501 Size: 7381 SacI G_AGCT'C Cuts at: 3387 3387 Size: 7381 SalI G'TCGA_C Cuts at: 1936 4723 1936 Size: 2787 4594 SanDI GG'GwC_CC Cuts at: 3990 4304 3990 Size: 314 7067 SapI GCTCTTCn'nnn_ Cuts at: 4025 4025 Size: 7381 SbfI CC_TGCA'GG Cuts at: 3603 4733 3603 Size: 1130 6251 ScaI AGT'ACT Cuts at: 4580 5629 7341 4580 Size: 1049 1712 4620 Fragments arranged by size: 4620 1712 1049 SfcI C'TryA_G Cuts at: 826 1045 3026 3599 4729 5864 6545 6736 826 Size: 219 1981 573 1130 1135 681 191 1471 Fragments arranged by size: 1981 1471 1135 1130 681 573 219 191 SfoI GGC'GCC Cuts at: 2966 2966 Size: 7381 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7381 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7381 SmaI CCC'GGG Cuts at: 1872 4217 4708 1872 Size: 2345 491 4545 Fragments arranged by size: 4545 2345 491 SmlI C'TyrA_G Cuts at: 820 1017 1943 3305 5485 6386 6648 820 Size: 197 926 1362 2180 901 262 1553 Fragments arranged by size: 2180 1553 1362 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5172 493 Size: 4679 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7381 SphI G_CATG'C Cuts at: 1132 2927 3872 4739 1132 Size: 1795 945 867 3774 Fragments arranged by size: 3774 1795 945 867 SspI AAT'ATT Cuts at: 5 52 5305 5 Size: 47 5253 2081 Fragments arranged by size: 5253 2081 47 StuI AGG'CCT Cuts at: 1517 3212 3227 3320 4436 1517 Size: 1695 15 93 1116 4462 Fragments arranged by size: 4462 1695 1116 93 15 StyI C'CwwG_G Cuts at: 513 1065 2004 2442 2982 3588 4131 4224 4536 Size: 552 939 438 540 606 543 93 312 Cuts at: 4536 4820 513 Size: 284 3074 Fragments arranged by size: 3074 939 606 552 543 540 438 312 284 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3427 3518 5408 5425 5578 5763 6208 Size: 1255 1847 91 1890 17 153 185 445 Cuts at: 6208 325 Size: 1498 Fragments arranged by size: 1890 1847 1498 1255 445 185 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3534 4578 5627 Size: 274 80 33 51 1443 1557 1044 1049 Cuts at: 5627 7339 96 Size: 1712 138 Fragments arranged by size: 1712 1557 1443 1049 1044 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2258 2290 4288 4320 7341 7373 2258 Size: 32 1998 32 3021 32 2266 Fragments arranged by size: 3021 2266 1998 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4487 1316 Size: 3171 4210 XbaI T'CTAG_A Cuts at: 4717 4717 Size: 7381 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2055 2750 2860 4129 2055 Size: 695 110 1269 5307 Fragments arranged by size: 5307 1269 695 110 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7381 XmaI C'CCGG_G Cuts at: 1870 4215 4706 1870 Size: 2345 491 4545 Fragments arranged by size: 4545 2345 491 XmnI GAAnn'nnTTC Cuts at: 4253 5510 4253 Size: 1257 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5189 276 Size: 53 83 186 936 3655 2468 Fragments arranged by size: 3655 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI SacII SexAI SfiI SrfI SwaI