################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeIII HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvaI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 14 AAATTT [GA]^AATT[CT] AcyI 209 GGCGCC G[GA]^CG[CT]C AflIII 60 ACATGT A^C[GA][CT]GT AluI 89 AGCT AG^CT AlwI 174 GATCC GGATC(4/5) ApoI 14 AAATTT [GA]^AATT[CT] BanI 209 GGCGCC G^G[CT][GA]CC BbeI 209 GGCGCC GGCGC^C BbvI 69 GCTGC GCAGC(8/12) BsaHI 209 GGCGCC G[GA]^CG[CT]C BsaJI 74 CCAAGG C^C[ACGT][ACGT]GG BseRI 43 GAGGAG GAGGAG(10/8) BsmFI 94 GTCCC GGGAC(10/14) BspLU11I 60 ACATGT A^CATGT BstF5I 157 GGATG GGATG(2/0) CfoI 210 GCGC GCG^C DpnII 174 GATC ^GATC EcoT14I 74 CCAAGG C^C[AT][AT]GG Fnu4HI 69 GCTGC GC^[ACGT]GC FokI 157 GGATG GGATG(9/13) HaeII 209 GGCGCC [GA]GCGC^[CT] HapII 207 CCGG C^CGG HhaI 210 GCGC GCG^C Hin1I 209 GGCGCC G[GA]^CG[CT]C HinP1I 210 GCGC G^CGC HpaII 207 CCGG C^CGG ItaI 69 GCTGC GC^[ACGT]GC KasI 209 GGCGCC G^GCGCC MboI 174 GATC ^GATC MspI 207 CCGG C^CGG MwoI 201 GCTGTTCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 209 GGCGCC GG^CGCC NdeII 174 GATC ^GATC NspI 60 ACATGT [GA]CATG^[CT] PleI 150 GAGTC GAGTC(4/5) Sau3AI 174 GATC ^GATC SfoI 209 GGCGCC GGC^GCC StyI 74 CCAAGG C^C[AT][AT]GG TaqI 57 TCGA T^CGA TseI 69 GCTGC G^C[AT]GC Tsp509I 15 AATT ^AATT TthHB8I 57 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### AvaII 93 GGTCC G^G[AT]CC 162 GGTCC Cfr13I 93 GGTCC G^G[ACGT]CC 162 GGTCC Sau96I 93 GGTCC G^G[ACGT]CC 162 GGTCC TfiI 118 GAATC G^A[AT]TC 132 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### HinfI MnlI NlaIII NlaIV