################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 940 GGTACC G^GTACC AcsI 61 AAATTT [GA]^AATT[CT] AcyI 67 GACGCC G[GA]^CG[CT]C Alw44I 145 GTGCAC G^TGCAC ApaLI 145 GTGCAC G^TGCAC ApoI 61 AAATTT [GA]^AATT[CT] Asp718I 940 GGTACC G^GTACC AspHI 145 GTGCAC G[AT]GC[AT]^C AspI 847 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 371 TGCGCA TGC^GCA BalI 242 TGGCCA TGG^CCA BbsI 672 GAAGAC GAAGAC(2/6) BglI 497 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 817 GCTGAGC GC^T[ACGT]AGC Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC BpuAI 672 GAAGAC GAAGAC(2/6) BsaAI 590 TACGTG [CT]AC^GT[GA] BsaHI 67 GACGCC G[GA]^CG[CT]C BsaI 454 GAGACC GGTCTC(1/5) BseRI 1010 GAGGAG GAGGAG(10/8) BsiEI 7 CGGCCG CG[GA][CT]^CG BsiHKAI 145 GTGCAC G[AT]GC[AT]^C BsmI 1004 GCATTC GAATGC(1/-1) BssHII 25 GCGCGC G^CGCGC BstXI 296 CCAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 817 GCTGAGC GC^T[ACGT]AGC EagI 7 CGGCCG C^GGCCG EarI 54 GAAGAG CTCTTC(1/4) EclXI 7 CGGCCG C^GGCCG Eco52I 7 CGGCCG C^GGCCG EcoT14I 677 CCTTGG C^C[AT][AT]GG FspI 371 TGCGCA TGC^GCA Hin1I 67 GACGCC G[GA]^CG[CT]C KpnI 940 GGTACC GGTAC^C Ksp632I 54 GAAGAG CTCTTC(1/4) MluNI 242 TGGCCA TGG^CCA MscI 242 TGGCCA TGG^CCA PaeR7I 643 CTCGAG C^TCGAG PflFI 847 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 546 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 54 GAAGAGC GCTCTTC(1/4) SexAI 848 ACCTGGT A^CC[AT]GGT SmaI 156 CCCGGG CCC^GGG SmlI 643 CTCGAG C^T[CT][GA]AG StyI 677 CCTTGG C^C[AT][AT]GG TfiI 569 GAATC G^A[AT]TC Tsp509I 62 AATT ^AATT Tth111I 847 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 546 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 643 CTCGAG C^TCGAG XmaCI 156 CCCGGG C^CCGGG XmaI 156 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AluI 288 AGCT AG^CT 949 AGCT AlwI 153 GATCC GGATC(4/5) 639 GATCC AlwNI 547 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 803 CAGGGCCTG AvaI 156 CCCGGG C^[CT]CG[GA]G 643 CTCGAG BanI 363 GGCACC G^G[CT][GA]CC 940 GGTACC BpmI 294 CTCCAG CTGGAG(16/14) 553 CTGGAG BsmAI 454 GAGAC GTCTC(1/5) 891 GTCTC BsmFI 911 GGGAC GGGAC(10/14) 32 GTCCC BsoBI 156 CCCGGG C^[CT]CG[GA]G 643 CTCGAG BspMI 200 ACCTGC ACCTGC(4/8) 914 ACCTGC BsrBI 108 GAGCGG CCGCTC(-3/-3) 556 GAGCGG BstYI 152 AGATCC [GA]^GATC[CT] 638 AGATCC DpnII 153 GATC ^GATC 639 GATC HgaI 67 GACGC GACGC(5/10) 80 GACGC MboI 153 GATC ^GATC 639 GATC MflI 152 AGATCC [GA]^GATC[CT] 638 AGATCC NdeII 153 GATC ^GATC 639 GATC PpuMI 789 AGGTCCT [GA]G^G[AT]CC[CT] 911 GGGACCT PstI 325 CTGCAG CTGCA^G 800 CTGCAG Sau3AI 153 GATC ^GATC 639 GATC SfcI 325 CTGCAG C^T[GA][CT]AG 800 CTGCAG XhoII 152 AGATCC [GA]^GATC[CT] 638 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AfaI ApaI AvaII BanII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I