(Linear) (Six-base) MAPSORT of: KIEE4587.seq Check: 3267 from: 1 to: 2679 KIEE4587.orf With 182 enzymes: SgfI * March 22, 2012 18:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 327 1395 2679 Size: 327 1068 1284 Fragments arranged by size: 1284 1068 327 AatII G_ACGT'C Cuts at: 0 1577 2679 Size: 1577 1102 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 29 696 2679 Size: 29 667 1983 Fragments arranged by size: 1983 667 29 AflIII A'CryG_T Cuts at: 0 71 2679 Size: 71 2608 AhdI GACnn_n'nnGTC Cuts at: 0 1920 2679 Size: 1920 759 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1183 1234 1870 2679 Size: 1183 51 636 809 Fragments arranged by size: 1183 809 636 51 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 941 973 2679 Size: 941 32 1706 Fragments arranged by size: 1706 941 32 AlwNI CAG_nnn'CTG Cuts at: 0 519 993 1017 2037 2358 2472 2571 2679 Size: 519 474 24 1020 321 114 99 108 Fragments arranged by size: 1020 519 474 321 114 108 99 24 ApaI G_GGCC'C Cuts at: 0 534 832 1008 1840 2325 2558 2597 2679 Size: 534 298 176 832 485 233 39 82 Fragments arranged by size: 832 534 485 298 233 176 82 39 AvaI C'yCGr_G Cuts at: 0 197 262 1261 1730 1865 1949 2318 2557 Size: 197 65 999 469 135 84 369 239 Cuts at: 2557 2679 Size: 122 Fragments arranged by size: 999 469 369 239 197 135 122 84 65 BanI G'GyrC_C Cuts at: 0 1347 2664 2679 Size: 1347 1317 15 Fragments arranged by size: 1347 1317 15 BanII G_rGCy'C Cuts at: 0 534 693 832 1008 1434 1840 2276 2325 Size: 534 159 139 176 426 406 436 49 Cuts at: 2325 2558 2597 2679 Size: 233 39 82 Fragments arranged by size: 534 436 426 406 233 176 159 139 82 49 39 BbeI G_GCGC'C Cuts at: 0 1351 2679 Size: 1351 1328 BbsI GAAGACnn'nnnn_ Cuts at: 0 1434 1872 2679 Size: 1434 438 807 Fragments arranged by size: 1434 807 438 BbvCI CC'TCA_GC Cuts at: 0 1000 2363 2679 Size: 1000 1363 316 Fragments arranged by size: 1363 1000 316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1704 1738 2167 2201 2679 Size: 1704 34 429 34 478 Fragments arranged by size: 1704 478 429 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1844 2679 Size: 1844 835 BlpI GC'TnA_GC Cuts at: 0 2270 2351 2679 Size: 2270 81 328 Fragments arranged by size: 2270 328 81 Bme1580I G_kGCm'C Cuts at: 0 534 815 832 1008 1388 1840 2325 2558 Size: 534 281 17 176 380 452 485 233 Cuts at: 2558 2597 2679 Size: 39 82 Fragments arranged by size: 534 485 452 380 281 233 176 82 39 17 BmgBI CAC'GTC Cuts at: 0 166 1917 2679 Size: 166 1751 762 Fragments arranged by size: 1751 762 166 BmrI ACTGGGnnnn_n' Cuts at: 0 743 2616 2679 Size: 743 1873 63 Fragments arranged by size: 1873 743 63 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1062 2366 2679 Size: 1062 1304 313 Fragments arranged by size: 1304 1062 313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 913 1039 1108 1321 2059 2146 2260 2350 Size: 913 126 69 213 738 87 114 90 Cuts at: 2350 2356 2449 2669 2679 Size: 6 93 220 10 Fragments arranged by size: 913 738 220 213 126 114 93 90 87 69 10 6 Bpu10I CC'TnA_GC Cuts at: 0 526 1000 1451 2363 2630 2679 Size: 526 474 451 912 267 49 Fragments arranged by size: 912 526 474 451 267 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2083 2624 2679 Size: 2083 541 55 Fragments arranged by size: 2083 541 55 BsaI GGTCTCn'nnnn_ Cuts at: 0 1050 1813 2679 Size: 1050 763 866 Fragments arranged by size: 1050 866 763 BsaAI yAC'GTr Cuts at: 0 449 777 2661 2679 Size: 449 328 1884 18 Fragments arranged by size: 1884 449 328 18 BsaHI Gr'CG_yC Cuts at: 0 650 1348 1574 1955 2679 Size: 650 698 226 381 724 Fragments arranged by size: 724 698 650 381 226 BsaWI w'CCGG_w Cuts at: 0 867 1183 1652 2679 Size: 867 316 469 1027 Fragments arranged by size: 1027 867 469 316 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 392 422 2164 2194 2679 Size: 392 30 1742 30 485 Fragments arranged by size: 1742 485 392 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 643 754 808 1036 1051 1138 1285 1489 Size: 643 111 54 228 15 87 147 204 Cuts at: 1489 2679 Size: 1190 Fragments arranged by size: 1190 643 228 204 147 111 87 54 15 BseYI C'CCAG_C Cuts at: 0 545 615 801 885 1057 1464 1504 1551 Size: 545 70 186 84 172 407 40 47 Cuts at: 1551 2324 2410 2437 2544 2596 2679 Size: 773 86 27 107 52 83 Fragments arranged by size: 773 545 407 186 172 107 86 84 83 70 52 47 40 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 664 778 1189 1423 1939 2679 Size: 159 505 114 411 234 516 740 Fragments arranged by size: 740 516 505 411 234 159 114 BsiEI CG_ry'CG Cuts at: 0 2058 2679 Size: 2058 621 BsiHKAI G_wGCw'C Cuts at: 0 2155 2276 2679 Size: 2155 121 403 Fragments arranged by size: 2155 403 121 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1663 2679 Size: 1663 1016 Bsp1286I G_dGCh'C Cuts at: 0 534 693 815 832 1008 1388 1434 1840 Size: 534 159 122 17 176 380 46 406 Cuts at: 1840 2155 2276 2325 2558 2597 2679 Size: 315 121 49 233 39 82 Fragments arranged by size: 534 406 380 315 233 176 159 122 121 82 49 46 39 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 327 1395 2442 2679 Size: 327 1068 1047 237 Fragments arranged by size: 1068 1047 327 237 BsrBI CCG'CTC Cuts at: 0 2067 2679 Size: 2067 612 BsrFI r'CCGG_y Cuts at: 0 2057 2679 Size: 2057 622 BssHII G'CGCG_C Cuts at: 0 1194 2679 Size: 1194 1485 BssSI C'ACGA_G Cuts at: 0 790 2679 Size: 790 1889 BstAPI GCAn_nnn'nTGC Cuts at: 0 542 993 2679 Size: 542 451 1686 Fragments arranged by size: 1686 542 451 BstEII G'GTnAC_C Cuts at: 0 150 591 2679 Size: 150 441 2088 Fragments arranged by size: 2088 441 150 BstXI CCAn_nnnn'nTGG Cuts at: 0 147 2679 Size: 147 2532 BstYI r'GATC_y Cuts at: 0 266 2075 2679 Size: 266 1809 604 Fragments arranged by size: 1809 604 266 Bsu36I CC'TnA_GG Cuts at: 0 1472 2679 Size: 1472 1207 BtgI C'CryG_G Cuts at: 0 1077 1745 2052 2144 2679 Size: 1077 668 307 92 535 Fragments arranged by size: 1077 668 535 307 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1707 2679 Size: 1707 972 BtsI GCAGTG_nn' Cuts at: 0 353 1799 1914 2679 Size: 353 1446 115 765 Fragments arranged by size: 1446 765 353 115 DraIII CAC_nnn'GTG Cuts at: 0 2664 2679 Size: 2664 15 DrdI GACnn_nn'nnGTC Cuts at: 0 1876 2679 Size: 1876 803 EaeI y'GGCC_r Cuts at: 0 182 1673 1979 2015 2055 2141 2252 2585 Size: 182 1491 306 36 40 86 111 333 Cuts at: 2585 2679 Size: 94 Fragments arranged by size: 1491 333 306 182 111 94 86 40 36 EagI C'GGCC_G Cuts at: 0 2055 2679 Size: 2055 624 EarI CTCTTCn'nnn_ Cuts at: 0 5 2527 2679 Size: 5 2522 152 Fragments arranged by size: 2522 152 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 711 1609 2185 2679 Size: 711 898 576 494 Fragments arranged by size: 898 711 576 494 EcoICRI GAG'CTC Cuts at: 0 2274 2679 Size: 2274 405 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 29 696 913 1039 1108 1321 2059 2146 Size: 29 667 217 126 69 213 738 87 Cuts at: 2146 2260 2350 2356 2449 2669 2679 Size: 114 90 6 93 220 10 Fragments arranged by size: 738 667 220 217 213 126 114 93 90 87 69 29 10 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1021 2679 Size: 1021 1658 EcoO109I rG'GnC_Cy Cuts at: 0 282 439 530 829 1004 1005 1293 1468 Size: 282 157 91 299 175 1 288 175 Cuts at: 1468 1555 1659 1836 1837 1862 1946 2407 2424 Size: 87 104 177 1 25 84 461 17 Cuts at: 2424 2554 2634 2679 Size: 130 80 45 Fragments arranged by size: 461 299 288 282 177 175 175 157 130 104 91 87 84 80 45 25 17 1 1 EcoRV GAT'ATC Cuts at: 0 2615 2679 Size: 2615 64 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 262 2679 Size: 262 2417 FspI TGC'GCA Cuts at: 0 145 2679 Size: 145 2534 FspAI rTGC'GCAy Cuts at: 0 145 2679 Size: 145 2534 HaeII r_GCGC'y Cuts at: 0 376 1131 1351 2679 Size: 376 755 220 1328 Fragments arranged by size: 1328 755 376 220 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1444 1476 1906 1938 2164 2196 2599 2631 Size: 1444 32 430 32 226 32 403 32 Cuts at: 2631 2679 Size: 48 Fragments arranged by size: 1444 430 403 226 48 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1347 2679 Size: 1347 1332 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 843 959 2679 Size: 843 116 1720 Fragments arranged by size: 1720 843 116 MscI TGG'CCA Cuts at: 0 184 1981 2254 2587 2679 Size: 184 1797 273 333 92 Fragments arranged by size: 1797 333 273 184 92 MslI CAynn'nnrTG Cuts at: 0 145 1165 1216 2143 2679 Size: 145 1020 51 927 536 Fragments arranged by size: 1020 927 536 145 51 MspA1I CmG'CkG Cuts at: 0 619 885 945 1079 1504 1620 1977 2054 Size: 619 266 60 134 425 116 357 77 Cuts at: 2054 2328 2358 2679 Size: 274 30 321 Fragments arranged by size: 619 425 357 321 274 266 134 116 77 60 30 NaeI GCC'GGC Cuts at: 0 2059 2679 Size: 2059 620 NarI GG'CG_CC Cuts at: 0 1348 2679 Size: 1348 1331 NgoMIV G'CCGG_C Cuts at: 0 2057 2679 Size: 2057 622 NspI r_CATG'y Cuts at: 0 1221 2679 Size: 1221 1458 PasI CC'CwG_GG Cuts at: 0 207 301 825 2472 2571 2602 2679 Size: 207 94 524 1647 99 31 77 Fragments arranged by size: 1647 524 207 99 94 77 31 PflMI CCAn_nnn'nTGG Cuts at: 0 54 213 1344 2472 2679 Size: 54 159 1131 1128 207 Fragments arranged by size: 1131 1128 207 159 54 PfoI T'CCnGG_A Cuts at: 0 704 959 2078 2646 2679 Size: 704 255 1119 568 33 Fragments arranged by size: 1119 704 568 255 33 PmlI CAC'GTG Cuts at: 0 777 2661 2679 Size: 777 1884 18 Fragments arranged by size: 1884 777 18 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1465 1497 2679 Size: 1465 32 1182 Fragments arranged by size: 1465 1182 32 PpuMI rG'GwC_Cy Cuts at: 0 282 439 1293 1468 1659 1946 2424 2679 Size: 282 157 854 175 191 287 478 255 Fragments arranged by size: 854 478 287 282 255 191 175 157 PspOMI G'GGCC_C Cuts at: 0 530 828 1004 1836 2321 2554 2593 2679 Size: 530 298 176 832 485 233 39 86 Fragments arranged by size: 832 530 485 298 233 176 86 39 PstI C_TGCA'G Cuts at: 0 338 1013 1619 2228 2679 Size: 338 675 606 609 451 Fragments arranged by size: 675 609 606 451 338 PvuII CAG'CTG Cuts at: 0 885 945 1504 1620 1977 2328 2358 2679 Size: 885 60 559 116 357 351 30 321 Fragments arranged by size: 885 559 357 351 321 116 60 30 RsrII CG'GwC_CG Cuts at: 0 865 2679 Size: 865 1814 SacI G_AGCT'C Cuts at: 0 2276 2679 Size: 2276 403 SacII CC_GC'GG Cuts at: 0 1080 2055 2679 Size: 1080 975 624 Fragments arranged by size: 1080 975 624 SbfI CC_TGCA'GG Cuts at: 0 338 2679 Size: 338 2341 SfcI C'TryA_G Cuts at: 0 334 1009 1615 2224 2679 Size: 334 675 606 609 455 Fragments arranged by size: 675 609 606 455 334 SfoI GGC'GCC Cuts at: 0 1349 2679 Size: 1349 1330 SmaI CCC'GGG Cuts at: 0 1263 1732 1867 1951 2320 2679 Size: 1263 469 135 84 369 359 Fragments arranged by size: 1263 469 369 359 135 84 SmlI C'TyrA_G Cuts at: 0 262 2062 2639 2679 Size: 262 1800 577 40 Fragments arranged by size: 1800 577 262 40 SrfI GCCC'GGGC Cuts at: 0 2320 2679 Size: 2320 359 StuI AGG'CCT Cuts at: 0 2248 2679 Size: 2248 431 StyI C'CwwG_G Cuts at: 0 1343 2165 2249 2679 Size: 1343 822 84 430 Fragments arranged by size: 1343 822 430 84 TatI w'GTAC_w Cuts at: 0 2670 2679 Size: 2670 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 392 424 2679 Size: 392 32 2255 Fragments arranged by size: 2255 392 32 Tth111I GACn'n_nGTC Cuts at: 0 652 859 1234 2679 Size: 652 207 375 1445 Fragments arranged by size: 1445 652 375 207 XbaI T'CTAG_A Cuts at: 0 1143 2679 Size: 1143 1536 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 822 1113 2679 Size: 822 291 1566 Fragments arranged by size: 1566 822 291 XhoI C'TCGA_G Cuts at: 0 262 2679 Size: 262 2417 XmaI C'CCGG_G Cuts at: 0 1261 1730 1865 1949 2318 2679 Size: 1261 469 135 84 369 361 Fragments arranged by size: 1261 469 369 361 135 84 XmnI GAAnn'nnTTC Cuts at: 0 2380 2679 Size: 2380 299 ZraI GAC'GTC Cuts at: 0 1575 2679 Size: 1575 1104 Enzymes that do cut: AarI AatII AcuI AflIII AhdI AlfI AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII SacI SacII SbfI SfcI SfoI SmaI SmlI SrfI StuI StyI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI BstZ17I ClaI CspCI DraI EcoRI FseI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TaqII