Restriction map for pFN21AE4587
 (Linear) (Six-base) MAPSORT of: KIEE4587.seq  Check: 3267  from: 1  to: 2679



KIEE4587.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     327    1395    2679 
   Size:        327    1068    1284

  Fragments arranged by size: 

                1284    1068     327
 
AatII G_ACGT'C

Cuts at:      0    1577    2679 
   Size:       1577    1102
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     696    2679 
   Size:         29     667    1983

  Fragments arranged by size: 

                1983     667      29
 
AflIII A'CryG_T

Cuts at:      0      71    2679 
   Size:         71    2608
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1920    2679 
   Size:       1920     759
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1183    1234    1870    2679 
   Size:       1183      51     636     809

  Fragments arranged by size: 

                1183     809     636      51
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     941     973    2679 
   Size:        941      32    1706

  Fragments arranged by size: 

                1706     941      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     519     993    1017    2037    2358    2472    2571    2679 
   Size:        519     474      24    1020     321     114      99     108

  Fragments arranged by size: 

                1020     519     474     321     114     108      99      24
            
 
ApaI G_GGCC'C

Cuts at:      0     534     832    1008    1840    2325    2558    2597    2679 
   Size:        534     298     176     832     485     233      39      82

  Fragments arranged by size: 

                 832     534     485     298     233     176      82      39
            
 
AvaI C'yCGr_G

Cuts at:      0     197     262    1261    1730    1865    1949    2318    2557 
   Size:        197      65     999     469     135      84     369     239

Cuts at:   2557    2679 
   Size:        122

  Fragments arranged by size: 

                 999     469     369     239     197     135     122      84
                  65
 
BanI G'GyrC_C

Cuts at:      0    1347    2664    2679 
   Size:       1347    1317      15

  Fragments arranged by size: 

                1347    1317      15
 
BanII G_rGCy'C

Cuts at:      0     534     693     832    1008    1434    1840    2276    2325 
   Size:        534     159     139     176     426     406     436      49

Cuts at:   2325    2558    2597    2679 
   Size:        233      39      82

  Fragments arranged by size: 

                 534     436     426     406     233     176     159     139
                  82      49      39
 
BbeI G_GCGC'C

Cuts at:      0    1351    2679 
   Size:       1351    1328
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1434    1872    2679 
   Size:       1434     438     807

  Fragments arranged by size: 

                1434     807     438
 
BbvCI CC'TCA_GC

Cuts at:      0    1000    2363    2679 
   Size:       1000    1363     316

  Fragments arranged by size: 

                1363    1000     316
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1704    1738    2167    2201    2679 
   Size:       1704      34     429      34     478

  Fragments arranged by size: 

                1704     478     429      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1844    2679 
   Size:       1844     835
 
BlpI GC'TnA_GC

Cuts at:      0    2270    2351    2679 
   Size:       2270      81     328

  Fragments arranged by size: 

                2270     328      81
 
Bme1580I G_kGCm'C

Cuts at:      0     534     815     832    1008    1388    1840    2325    2558 
   Size:        534     281      17     176     380     452     485     233

Cuts at:   2558    2597    2679 
   Size:         39      82

  Fragments arranged by size: 

                 534     485     452     380     281     233     176      82
                  39      17
 
BmgBI CAC'GTC

Cuts at:      0     166    1917    2679 
   Size:        166    1751     762

  Fragments arranged by size: 

                1751     762     166
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     743    2616    2679 
   Size:        743    1873      63

  Fragments arranged by size: 

                1873     743      63
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1062    2366    2679 
   Size:       1062    1304     313

  Fragments arranged by size: 

                1304    1062     313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     913    1039    1108    1321    2059    2146    2260    2350 
   Size:        913     126      69     213     738      87     114      90

Cuts at:   2350    2356    2449    2669    2679 
   Size:          6      93     220      10

  Fragments arranged by size: 

                 913     738     220     213     126     114      93      90
                  87      69      10       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     526    1000    1451    2363    2630    2679 
   Size:        526     474     451     912     267      49

  Fragments arranged by size: 

                 912     526     474     451     267      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2083    2624    2679 
   Size:       2083     541      55

  Fragments arranged by size: 

                2083     541      55
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1050    1813    2679 
   Size:       1050     763     866

  Fragments arranged by size: 

                1050     866     763
 
BsaAI yAC'GTr

Cuts at:      0     449     777    2661    2679 
   Size:        449     328    1884      18

  Fragments arranged by size: 

                1884     449     328      18
 
BsaHI Gr'CG_yC

Cuts at:      0     650    1348    1574    1955    2679 
   Size:        650     698     226     381     724

  Fragments arranged by size: 

                 724     698     650     381     226
 
BsaWI w'CCGG_w

Cuts at:      0     867    1183    1652    2679 
   Size:        867     316     469    1027

  Fragments arranged by size: 

                1027     867     469     316
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     392     422    2164    2194    2679 
   Size:        392      30    1742      30     485

  Fragments arranged by size: 

                1742     485     392      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     643     754     808    1036    1051    1138    1285    1489 
   Size:        643     111      54     228      15      87     147     204

Cuts at:   1489    2679 
   Size:       1190

  Fragments arranged by size: 

                1190     643     228     204     147     111      87      54
                  15
 
BseYI C'CCAG_C

Cuts at:      0     545     615     801     885    1057    1464    1504    1551 
   Size:        545      70     186      84     172     407      40      47

Cuts at:   1551    2324    2410    2437    2544    2596    2679 
   Size:        773      86      27     107      52      83

  Fragments arranged by size: 

                 773     545     407     186     172     107      86      84
                  83      70      52      47      40      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     664     778    1189    1423    1939    2679 
   Size:        159     505     114     411     234     516     740

  Fragments arranged by size: 

                 740     516     505     411     234     159     114
 
BsiEI CG_ry'CG

Cuts at:      0    2058    2679 
   Size:       2058     621
 
BsiHKAI G_wGCw'C

Cuts at:      0    2155    2276    2679 
   Size:       2155     121     403

  Fragments arranged by size: 

                2155     403     121
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1663    2679 
   Size:       1663    1016
 
Bsp1286I G_dGCh'C

Cuts at:      0     534     693     815     832    1008    1388    1434    1840 
   Size:        534     159     122      17     176     380      46     406

Cuts at:   1840    2155    2276    2325    2558    2597    2679 
   Size:        315     121      49     233      39      82

  Fragments arranged by size: 

                 534     406     380     315     233     176     159     122
                 121      82      49      46      39      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     327    1395    2442    2679 
   Size:        327    1068    1047     237

  Fragments arranged by size: 

                1068    1047     327     237
 
BsrBI CCG'CTC

Cuts at:      0    2067    2679 
   Size:       2067     612
 
BsrFI r'CCGG_y

Cuts at:      0    2057    2679 
   Size:       2057     622
 
BssHII G'CGCG_C

Cuts at:      0    1194    2679 
   Size:       1194    1485
 
BssSI C'ACGA_G

Cuts at:      0     790    2679 
   Size:        790    1889
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     542     993    2679 
   Size:        542     451    1686

  Fragments arranged by size: 

                1686     542     451
 
BstEII G'GTnAC_C

Cuts at:      0     150     591    2679 
   Size:        150     441    2088

  Fragments arranged by size: 

                2088     441     150
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147    2679 
   Size:        147    2532
 
BstYI r'GATC_y

Cuts at:      0     266    2075    2679 
   Size:        266    1809     604

  Fragments arranged by size: 

                1809     604     266
 
Bsu36I CC'TnA_GG

Cuts at:      0    1472    2679 
   Size:       1472    1207
 
BtgI C'CryG_G

Cuts at:      0    1077    1745    2052    2144    2679 
   Size:       1077     668     307      92     535

  Fragments arranged by size: 

                1077     668     535     307      92
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1707    2679 
   Size:       1707     972
 
BtsI GCAGTG_nn'

Cuts at:      0     353    1799    1914    2679 
   Size:        353    1446     115     765

  Fragments arranged by size: 

                1446     765     353     115
 
DraIII CAC_nnn'GTG

Cuts at:      0    2664    2679 
   Size:       2664      15
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1876    2679 
   Size:       1876     803
 
EaeI y'GGCC_r

Cuts at:      0     182    1673    1979    2015    2055    2141    2252    2585 
   Size:        182    1491     306      36      40      86     111     333

Cuts at:   2585    2679 
   Size:         94

  Fragments arranged by size: 

                1491     333     306     182     111      94      86      40
                  36
 
EagI C'GGCC_G

Cuts at:      0    2055    2679 
   Size:       2055     624
 
EarI CTCTTCn'nnn_

Cuts at:      0       5    2527    2679 
   Size:          5    2522     152

  Fragments arranged by size: 

                2522     152       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     711    1609    2185    2679 
   Size:        711     898     576     494

  Fragments arranged by size: 

                 898     711     576     494
 
EcoICRI GAG'CTC

Cuts at:      0    2274    2679 
   Size:       2274     405
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     696     913    1039    1108    1321    2059    2146 
   Size:         29     667     217     126      69     213     738      87

Cuts at:   2146    2260    2350    2356    2449    2669    2679 
   Size:        114      90       6      93     220      10

  Fragments arranged by size: 

                 738     667     220     217     213     126     114      93
                  90      87      69      29      10       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1021    2679 
   Size:       1021    1658
 
EcoO109I rG'GnC_Cy

Cuts at:      0     282     439     530     829    1004    1005    1293    1468 
   Size:        282     157      91     299     175       1     288     175

Cuts at:   1468    1555    1659    1836    1837    1862    1946    2407    2424 
   Size:         87     104     177       1      25      84     461      17

Cuts at:   2424    2554    2634    2679 
   Size:        130      80      45

  Fragments arranged by size: 

                 461     299     288     282     177     175     175     157
                 130     104      91      87      84      80      45      25
                  17       1       1
 
EcoRV GAT'ATC

Cuts at:      0    2615    2679 
   Size:       2615      64
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     262    2679 
   Size:        262    2417
 
FspI TGC'GCA

Cuts at:      0     145    2679 
   Size:        145    2534
 
FspAI rTGC'GCAy

Cuts at:      0     145    2679 
   Size:        145    2534
 
HaeII r_GCGC'y

Cuts at:      0     376    1131    1351    2679 
   Size:        376     755     220    1328

  Fragments arranged by size: 

                1328     755     376     220
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1444    1476    1906    1938    2164    2196    2599    2631 
   Size:       1444      32     430      32     226      32     403      32

Cuts at:   2631    2679 
   Size:         48

  Fragments arranged by size: 

                1444     430     403     226      48      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0    1347    2679 
   Size:       1347    1332
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     843     959    2679 
   Size:        843     116    1720

  Fragments arranged by size: 

                1720     843     116
 
MscI TGG'CCA

Cuts at:      0     184    1981    2254    2587    2679 
   Size:        184    1797     273     333      92

  Fragments arranged by size: 

                1797     333     273     184      92
 
MslI CAynn'nnrTG

Cuts at:      0     145    1165    1216    2143    2679 
   Size:        145    1020      51     927     536

  Fragments arranged by size: 

                1020     927     536     145      51
 
MspA1I CmG'CkG

Cuts at:      0     619     885     945    1079    1504    1620    1977    2054 
   Size:        619     266      60     134     425     116     357      77

Cuts at:   2054    2328    2358    2679 
   Size:        274      30     321

  Fragments arranged by size: 

                 619     425     357     321     274     266     134     116
                  77      60      30
 
NaeI GCC'GGC

Cuts at:      0    2059    2679 
   Size:       2059     620
 
NarI GG'CG_CC

Cuts at:      0    1348    2679 
   Size:       1348    1331
 
NgoMIV G'CCGG_C

Cuts at:      0    2057    2679 
   Size:       2057     622
 
NspI r_CATG'y

Cuts at:      0    1221    2679 
   Size:       1221    1458
 
PasI CC'CwG_GG

Cuts at:      0     207     301     825    2472    2571    2602    2679 
   Size:        207      94     524    1647      99      31      77

  Fragments arranged by size: 

                1647     524     207      99      94      77      31
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      54     213    1344    2472    2679 
   Size:         54     159    1131    1128     207

  Fragments arranged by size: 

                1131    1128     207     159      54
 
PfoI T'CCnGG_A

Cuts at:      0     704     959    2078    2646    2679 
   Size:        704     255    1119     568      33

  Fragments arranged by size: 

                1119     704     568     255      33
 
PmlI CAC'GTG

Cuts at:      0     777    2661    2679 
   Size:        777    1884      18

  Fragments arranged by size: 

                1884     777      18
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1465    1497    2679 
   Size:       1465      32    1182

  Fragments arranged by size: 

                1465    1182      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     282     439    1293    1468    1659    1946    2424    2679 
   Size:        282     157     854     175     191     287     478     255

  Fragments arranged by size: 

                 854     478     287     282     255     191     175     157
            
 
PspOMI G'GGCC_C

Cuts at:      0     530     828    1004    1836    2321    2554    2593    2679 
   Size:        530     298     176     832     485     233      39      86

  Fragments arranged by size: 

                 832     530     485     298     233     176      86      39
            
 
PstI C_TGCA'G

Cuts at:      0     338    1013    1619    2228    2679 
   Size:        338     675     606     609     451

  Fragments arranged by size: 

                 675     609     606     451     338
 
PvuII CAG'CTG

Cuts at:      0     885     945    1504    1620    1977    2328    2358    2679 
   Size:        885      60     559     116     357     351      30     321

  Fragments arranged by size: 

                 885     559     357     351     321     116      60      30
            
 
RsrII CG'GwC_CG

Cuts at:      0     865    2679 
   Size:        865    1814
 
SacI G_AGCT'C

Cuts at:      0    2276    2679 
   Size:       2276     403
 
SacII CC_GC'GG

Cuts at:      0    1080    2055    2679 
   Size:       1080     975     624

  Fragments arranged by size: 

                1080     975     624
 
SbfI CC_TGCA'GG

Cuts at:      0     338    2679 
   Size:        338    2341
 
SfcI C'TryA_G

Cuts at:      0     334    1009    1615    2224    2679 
   Size:        334     675     606     609     455

  Fragments arranged by size: 

                 675     609     606     455     334
 
SfoI GGC'GCC

Cuts at:      0    1349    2679 
   Size:       1349    1330
 
SmaI CCC'GGG

Cuts at:      0    1263    1732    1867    1951    2320    2679 
   Size:       1263     469     135      84     369     359

  Fragments arranged by size: 

                1263     469     369     359     135      84
 
SmlI C'TyrA_G

Cuts at:      0     262    2062    2639    2679 
   Size:        262    1800     577      40

  Fragments arranged by size: 

                1800     577     262      40
 
SrfI GCCC'GGGC

Cuts at:      0    2320    2679 
   Size:       2320     359
 
StuI AGG'CCT

Cuts at:      0    2248    2679 
   Size:       2248     431
 
StyI C'CwwG_G

Cuts at:      0    1343    2165    2249    2679 
   Size:       1343     822      84     430

  Fragments arranged by size: 

                1343     822     430      84
 
TatI w'GTAC_w

Cuts at:      0    2670    2679 
   Size:       2670       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     392     424    2679 
   Size:        392      32    2255

  Fragments arranged by size: 

                2255     392      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     652     859    1234    2679 
   Size:        652     207     375    1445

  Fragments arranged by size: 

                1445     652     375     207
 
XbaI T'CTAG_A

Cuts at:      0    1143    2679 
   Size:       1143    1536
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     822    1113    2679 
   Size:        822     291    1566

  Fragments arranged by size: 

                1566     822     291
 
XhoI C'TCGA_G

Cuts at:      0     262    2679 
   Size:        262    2417
 
XmaI C'CCGG_G

Cuts at:      0    1261    1730    1865    1949    2318    2679 
   Size:       1261     469     135      84     369     361

  Fragments arranged by size: 

                1261     469     369     361     135      84
 
XmnI GAAnn'nnTTC

Cuts at:      0    2380    2679 
   Size:       2380     299
 
ZraI GAC'GTC

Cuts at:      0    1575    2679 
   Size:       1575    1104
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AhdI     AlfI     AloI    AlwNI
     ApaI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    FspAI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI     PpiI
    PpuMI   PspOMI     PstI    PvuII    RsrII     SacI    SacII     SbfI
     SfcI     SfoI     SmaI     SmlI     SrfI     StuI     StyI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI
     BclI    BfrBI    BglII     BmtI    BsaBI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI    BstBI  BstZ17I     ClaI    CspCI     DraI
    EcoRI     FseI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI    PshAI     PsiI    PspXI     PsrI     PvuI     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     SwaI    TaqII
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