################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AccI AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AviII BbrPI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 82 GACGTC GACGT^C Acc65I 764 GGTACC G^GTACC AcsI 277 GAATTC [GA]^AATT[CT] AhdI 843 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 246 GGGCCC GGGCC^C ApoI 277 GAATTC [GA]^AATT[CT] Asp718I 764 GGTACC G^GTACC AspEI 843 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 82 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 951 CCTAGG C^CTAGG BamHI 1227 GGATCC G^GATCC BbsI 229 GTCTTC GAAGAC(2/6) BcgI 566 CGAGCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 736 GTATCC GTATCC(6/5) BfaI 952 CTAG C^TAG BlnI 951 CCTAGG C^CTAGG BpmI 470 CTGGAG CTGGAG(16/14) BpuAI 229 GTCTTC GAAGAC(2/6) BsgI 91 GTGCAG GTGCAG(16/14) BsmBI 498 CGTCTC CGTCTC(1/5) BsmI 312 GAATGC GAATGC(1/-1) BssSI 673 CACGAG CACGAG(-5/-1) BstYI 1227 GGATCC [GA]^GATC[CT] DrdI 174 GACGCATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 843 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1218 CTCTTC CTCTTC(1/4) EcoNI 317 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 277 GAATTC G^AATTC EcoRV 406 GATATC GAT^ATC KpnI 764 GGTACC GGTAC^C Ksp632I 1218 CTCTTC CTCTTC(1/4) MaeI 952 CTAG C^TAG MflI 1227 GGATCC [GA]^GATC[CT] MseI 928 TTAA T^TAA PaeR7I 1146 CTCGAG C^TCGAG PflFI 82 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 608 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 956 GAGCTC GAGCT^C SapI 1217 GCTCTTC GCTCTTC(1/4) SfcI 151 CTACAG C^T[GA][CT]AG SphI 1158 GCATGC GCATG^C TaqI 1147 TCGA T^CGA Tru9I 928 TTAA T^TAA Tth111I 82 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1147 TCGA T^CGA Van91I 608 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1031 CCATCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1146 CTCGAG C^TCGAG XhoII 1227 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaI 5 CCCGAG C^[CT]CG[GA]G 1146 CTCGAG BalI 61 TGGCCA TGG^CCA 771 TGGCCA BbeI 96 GGCGCC GGCGC^C 880 GGCGCC BcnI 77 CCGGG CC^[GC]GG 112 CCCGG BsmAI 499 GTCTC GTCTC(1/5) 1186 GTCTC BsoBI 5 CCCGAG C^[CT]CG[GA]G 1146 CTCGAG BstXI 266 CCAAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 462 CCAGCTCACTGG HapII 77 CCGG C^CGG 113 CCGG HpaII 77 CCGG C^CGG 113 CCGG KasI 96 GGCGCC G^GCGCC 880 GGCGCC MluNI 61 TGGCCA TGG^CCA 771 TGGCCA MscI 61 TGGCCA TGG^CCA 771 TGGCCA MspI 77 CCGG C^CGG 113 CCGG NarI 96 GGCGCC GG^CGCC 880 GGCGCC NciI 77 CCGGG CC^[GC]GG 112 CCCGG PleI 127 GACTC GAGTC(4/5) 262 GAGTC SfaNI 555 GATGC GCATC(5/9) 799 GATGC SfoI 96 GGCGCC GGC^GCC 880 GGCGCC SmlI 630 CTCAAG C^T[CT][GA]AG 1146 CTCGAG TfiI 211 GAATC G^A[AT]TC 1052 GAATC Tsp509I 278 AATT ^AATT 832 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvCI BbvI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HgaI HhaI Hin1I HinP1I HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI StyI TseI Tsp45I TspRI