(Linear) (Six-base) MAPSORT of: KIEE4396.seq Check: 7273 from: 1 to: 1251 KIEE4396.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 373 1251 Size: 373 878 AatII G_ACGT'C Cuts at: 0 86 1251 Size: 86 1165 Acc65I G'GTAC_C Cuts at: 0 764 1251 Size: 764 487 AhdI GACnn_n'nnGTC Cuts at: 0 848 1251 Size: 848 403 AleI CACnn'nnGTG Cuts at: 0 106 1251 Size: 106 1145 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 962 994 1204 1236 1251 Size: 962 32 210 32 15 Fragments arranged by size: 962 210 32 32 15 AlwNI CAG_nnn'CTG Cuts at: 0 601 614 699 891 1251 Size: 601 13 85 192 360 Fragments arranged by size: 601 360 192 85 13 ApaI G_GGCC'C Cuts at: 0 250 1251 Size: 250 1001 ApoI r'AATT_y Cuts at: 0 277 1251 Size: 277 974 AvaI C'yCGr_G Cuts at: 0 5 1146 1251 Size: 5 1141 105 Fragments arranged by size: 1141 105 5 AvrII C'CTAG_G Cuts at: 0 951 1251 Size: 951 300 BamHI G'GATC_C Cuts at: 0 1227 1251 Size: 1227 24 BanI G'GyrC_C Cuts at: 0 96 108 156 573 624 764 880 1251 Size: 96 12 48 417 51 140 116 371 Fragments arranged by size: 417 371 140 116 96 51 48 12 BanII G_rGCy'C Cuts at: 0 250 821 960 1251 Size: 250 571 139 291 Fragments arranged by size: 571 291 250 139 BbeI G_GCGC'C Cuts at: 0 100 884 1251 Size: 100 784 367 Fragments arranged by size: 784 367 100 BbsI GAAGACnn'nnnn_ Cuts at: 0 222 1251 Size: 222 1029 BbvCI CC'TCA_GC Cuts at: 0 322 446 692 1170 1251 Size: 322 124 246 478 81 Fragments arranged by size: 478 322 246 124 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 555 589 1251 Size: 555 34 662 Fragments arranged by size: 662 555 34 BciVI GTATCCnnnnn_n' Cuts at: 0 747 1251 Size: 747 504 Bme1580I G_kGCm'C Cuts at: 0 113 161 250 485 629 1246 1251 Size: 113 48 89 235 144 617 5 Fragments arranged by size: 617 235 144 113 89 48 5 BmrI ACTGGGnnnn_n' Cuts at: 0 304 995 1251 Size: 304 691 256 Fragments arranged by size: 691 304 256 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1233 1251 Size: 1233 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 1251 Size: 491 760 Bpu10I CC'TnA_GC Cuts at: 0 322 446 692 1170 1251 Size: 322 124 246 478 81 Fragments arranged by size: 478 322 246 124 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 615 1251 Size: 615 636 BsaHI Gr'CG_yC Cuts at: 0 83 97 881 1251 Size: 83 14 784 370 Fragments arranged by size: 784 370 83 14 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 464 494 1251 Size: 464 30 757 Fragments arranged by size: 757 464 30 BseYI C'CCAG_C Cuts at: 0 250 884 1074 1251 Size: 250 634 190 177 Fragments arranged by size: 634 250 190 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 1251 Size: 112 1139 BsiHKAI G_wGCw'C Cuts at: 0 674 841 960 1010 1251 Size: 674 167 119 50 241 Fragments arranged by size: 674 241 167 119 50 BsmI GAATG_Cn' Cuts at: 0 318 1251 Size: 318 933 BsmBI CGTCTCn'nnnn_ Cuts at: 0 504 1251 Size: 504 747 Bsp1286I G_dGCh'C Cuts at: 0 113 161 250 485 629 674 821 841 Size: 113 48 89 235 144 45 147 20 Cuts at: 841 960 1010 1246 1251 Size: 119 50 236 5 Fragments arranged by size: 236 235 147 144 119 113 89 50 48 45 20 5 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 373 667 802 1251 Size: 373 294 135 449 Fragments arranged by size: 449 373 294 135 BssSI C'ACGA_G Cuts at: 0 673 1251 Size: 673 578 BstXI CCAn_nnnn'nTGG Cuts at: 0 273 469 1251 Size: 273 196 782 Fragments arranged by size: 782 273 196 BstYI r'GATC_y Cuts at: 0 1227 1251 Size: 1227 24 BtgI C'CryG_G Cuts at: 0 11 485 845 1251 Size: 11 474 360 406 Fragments arranged by size: 474 406 360 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 812 1251 Size: 812 439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 620 655 1251 Size: 620 35 596 Fragments arranged by size: 620 596 35 DrdI GACnn_nn'nnGTC Cuts at: 0 180 1251 Size: 180 1071 EaeI y'GGCC_r Cuts at: 0 14 25 61 224 771 1251 Size: 14 11 36 163 547 480 Fragments arranged by size: 547 480 163 36 14 11 EarI CTCTTCn'nnn_ Cuts at: 0 1224 1251 Size: 1224 27 EcoICRI GAG'CTC Cuts at: 0 958 1251 Size: 958 293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 1251 Size: 491 760 EcoNI CCTnn'n_nnAGG Cuts at: 0 321 1251 Size: 321 930 EcoO109I rG'GnC_Cy Cuts at: 0 246 247 850 994 1119 1251 Size: 246 1 603 144 125 132 Fragments arranged by size: 603 246 144 132 125 1 EcoRI G'AATT_C Cuts at: 0 277 1251 Size: 277 974 EcoRV GAT'ATC Cuts at: 0 408 1251 Size: 408 843 HaeII r_GCGC'y Cuts at: 0 46 100 884 1169 1251 Size: 46 54 784 285 82 Fragments arranged by size: 784 285 82 54 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 834 866 1251 Size: 834 32 385 Fragments arranged by size: 834 385 32 KasI G'GCGC_C Cuts at: 0 96 880 1251 Size: 96 784 371 Fragments arranged by size: 784 371 96 KpnI G_GTAC'C Cuts at: 0 768 1251 Size: 768 483 MscI TGG'CCA Cuts at: 0 63 773 1251 Size: 63 710 478 Fragments arranged by size: 710 478 63 MslI CAynn'nnrTG Cuts at: 0 106 169 844 1251 Size: 106 63 675 407 Fragments arranged by size: 675 407 106 63 MspA1I CmG'CkG Cuts at: 0 30 189 445 494 696 1198 1251 Size: 30 159 256 49 202 502 53 Fragments arranged by size: 502 256 202 159 53 49 30 NarI GG'CG_CC Cuts at: 0 97 881 1251 Size: 97 784 370 Fragments arranged by size: 784 370 97 NcoI C'CATG_G Cuts at: 0 11 485 845 1251 Size: 11 474 360 406 Fragments arranged by size: 474 406 360 11 NspI r_CATG'y Cuts at: 0 181 1020 1162 1186 1251 Size: 181 839 142 24 65 Fragments arranged by size: 839 181 142 65 24 PasI CC'CwG_GG Cuts at: 0 876 1251 Size: 876 375 PflMI CCAn_nnn'nTGG Cuts at: 0 614 1251 Size: 614 637 PpuMI rG'GwC_Cy Cuts at: 0 850 994 1119 1251 Size: 850 144 125 132 Fragments arranged by size: 850 144 132 125 PspOMI G'GGCC_C Cuts at: 0 246 1251 Size: 246 1005 PspXI vC'TCGA_Gb Cuts at: 0 1146 1251 Size: 1146 105 PvuII CAG'CTG Cuts at: 0 189 445 696 1198 1251 Size: 189 256 251 502 53 Fragments arranged by size: 502 256 251 189 53 SacI G_AGCT'C Cuts at: 0 960 1251 Size: 960 291 SanDI GG'GwC_CC Cuts at: 0 850 1251 Size: 850 401 SapI GCTCTTCn'nnn_ Cuts at: 0 1224 1251 Size: 1224 27 SfcI C'TryA_G Cuts at: 0 151 1251 Size: 151 1100 SfoI GGC'GCC Cuts at: 0 98 882 1251 Size: 98 784 369 Fragments arranged by size: 784 369 98 SmlI C'TyrA_G Cuts at: 0 630 1146 1251 Size: 630 516 105 Fragments arranged by size: 630 516 105 SphI G_CATG'C Cuts at: 0 1162 1251 Size: 1162 89 StyI C'CwwG_G Cuts at: 0 11 485 768 845 951 1251 Size: 11 474 283 77 106 300 Fragments arranged by size: 474 300 283 106 77 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 118 1251 Size: 118 1133 TatI w'GTAC_w Cuts at: 0 719 1150 1251 Size: 719 431 101 Fragments arranged by size: 719 431 101 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 464 496 1251 Size: 464 32 755 Fragments arranged by size: 755 464 32 Tth111I GACn'n_nGTC Cuts at: 0 85 1251 Size: 85 1166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1038 1251 Size: 1038 213 XhoI C'TCGA_G Cuts at: 0 1146 1251 Size: 1146 105 ZraI GAC'GTC Cuts at: 0 84 1251 Size: 84 1167 Enzymes that do cut: AarI AatII Acc65I AhdI AleI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BssSI BstXI BstYI BtgI BtgZI CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I KasI KpnI MscI MslI MspA1I NarI NcoI NspI PasI PflMI PpuMI PspOMI PspXI PvuII SacI SanDI SapI SfcI SfoI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XhoI ZraI Enzymes that do not cut: AccI AclI AcuI AfeI AflII AflIII AgeI AlfI ApaLI AscI AseI AsiSI BaeI BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI DraIII EagI EciI FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XbaI XmaI XmnI