################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 576 GACGTC GACGT^C AccI 106 GTCTAC GT^[AC][GT]AC AcsI 289 AAATTC [GA]^AATT[CT] AcyI 576 GACGTC G[GA]^CG[CT]C Alw44I 448 GTGCAC G^TGCAC ApaLI 448 GTGCAC G^TGCAC ApoI 289 AAATTC [GA]^AATT[CT] BalI 33 TGGCCA TGG^CCA BamHI 512 GGATCC G^GATCC BsaHI 576 GACGTC G[GA]^CG[CT]C BsgI 202 CTGCAC GTGCAG(16/14) BsmAI 348 GTCTC GTCTC(1/5) BsrFI 150 GCCGGT [GA]^CCGG[CT] BsrI 280 CCAGT ACTGG(1/-1) BssHII 615 GCGCGC G^CGCGC BssSI 453 CACGAG CACGAG(-5/-1) Cac8I 615 GCGCGC GC[ACGT]^[ACGT]GC Cfr10I 150 GCCGGT [GA]^CCGG[CT] DdeI 333 CTCAG C^T[ACGT]AG DraI 588 TTTAAA TTT^AAA DsaI 113 CCGTGG C^C[GA][CT]GG EarI 610 GAAGAG CTCTTC(1/4) EcoT22I 541 ATGCAT ATGCA^T HgaI 209 GCGTC GACGC(5/10) Hin1I 576 GACGTC G[GA]^CG[CT]C Ksp632I 610 GAAGAG CTCTTC(1/4) MluNI 33 TGGCCA TGG^CCA MscI 33 TGGCCA TGG^CCA MseI 589 TTAA T^TAA MslI 538 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 361 CAGCTG C[AC]G^C[GT]G MwoI 312 GCCTCCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 512 GGATCC GG[ACGT]^[ACGT]CC NsiI 541 ATGCAT ATGCA^T NspI 539 ACATGC [GA]CATG^[CT] PflMI 36 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 337 GACTC GAGTC(4/5) PvuII 361 CAGCTG CAG^CTG SapI 610 GAAGAGC GCTCTTC(1/4) TfiI 180 GAATC G^A[AT]TC Tru9I 589 TTAA T^TAA Tsp509I 290 AATT ^AATT Van91I 36 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 467 CCACTCGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccII 100 CGCG CG^CG 616 CGCG AfaI 141 GTAC GT^AC 431 GTAC AvaII 153 GGTCC G^G[AT]CC 411 GGACC BpmI 67 CTGGAG CTGGAG(16/14) 223 CTGGAG BsaAI 94 TACGTG [CT]AC^GT[GA] 604 TACGTG BstUI 100 CGCG CG^CG 616 CGCG BstYI 485 AGATCC [GA]^GATC[CT] 512 GGATCC EaeI 33 TGGCCA [CT]^GGCC[GA] 44 TGGCCG HinfI 180 GAATC G^A[ACGT]TC 337 GACTC HphI 255 GGTGA GGTGA(8/7) 305 TCACC MflI 485 AGATCC [GA]^GATC[CT] 512 GGATCC MvnI 100 CGCG CG^CG 616 CGCG RsaI 141 GTAC GT^AC 431 GTAC XhoII 485 AGATCC [GA]^GATC[CT] 512 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AlwI AspHI BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MboII MnlI MspI MvaI NciI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI TseI TspRI