(Circular) (Six-base) MAPSORT of: pFN21AE9658.seq Check: 8873 from: 1 to: 5338 pFN21AE9658 5338 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2585 3148 278 Size: 53 83 186 936 1049 563 2468 Fragments arranged by size: 2468 1049 936 563 186 83 53 AccI GT'mk_AC Cuts at: 1937 2112 2681 1937 Size: 175 569 4594 Fragments arranged by size: 4594 569 175 Acc65I G'GTAC_C Cuts at: 2659 2659 Size: 5338 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 3401 4785 5053 1632 Size: 776 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 776 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5141 AflIII A'CryG_T Cuts at: 1203 4237 5214 1203 Size: 3034 977 1327 Fragments arranged by size: 3034 1327 977 AgeI A'CCGG_T Cuts at: 4169 4169 Size: 5338 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5338 AlwNI CAG_nnn'CTG Cuts at: 3081 4653 3081 Size: 1572 3766 ApaLI G'TGCA_C Cuts at: 2453 3395 4551 2453 Size: 942 1156 3240 Fragments arranged by size: 3240 1156 942 ApoI r'AATT_y Cuts at: 1493 2294 2647 2873 3025 5134 1493 Size: 801 353 226 152 2109 1697 Fragments arranged by size: 2109 1697 801 353 226 152 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2663 1870 Size: 73 720 4545 Fragments arranged by size: 4545 720 73 BamHI G'GATC_C Cuts at: 2517 2668 3035 2517 Size: 151 367 4820 Fragments arranged by size: 4820 367 151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2659 4114 618 Size: 325 217 627 872 1455 1842 Fragments arranged by size: 1842 1455 872 627 325 217 BanII G_rGCy'C Cuts at: 1391 2657 1391 Size: 1266 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3529 3563 1903 Size: 34 1592 34 3678 Fragments arranged by size: 3678 1592 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BfrBI ATG'CAT Cuts at: 2548 2548 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3949 136 Size: 107 122 71 631 605 2277 1525 Fragments arranged by size: 2277 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 2457 3399 4555 1790 Size: 667 942 1156 2573 Fragments arranged by size: 2573 1156 942 667 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2279 3069 4027 Size: 416 53 83 399 24 862 790 958 Cuts at: 4027 442 Size: 1753 Fragments arranged by size: 1753 958 862 790 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2249 3998 1239 Size: 854 156 1749 2579 Fragments arranged by size: 2579 1749 854 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4328 4626 3463 Size: 865 298 4175 Fragments arranged by size: 4175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4001 882 Size: 617 2502 2219 Fragments arranged by size: 2502 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2101 2611 3129 493 Size: 711 897 510 518 2702 Fragments arranged by size: 2702 897 711 518 510 BsaBI GATnn'nnATC Cuts at: 5332 5332 Size: 5338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2582 3145 3527 4162 Size: 53 83 186 936 1049 563 382 635 Cuts at: 4162 275 Size: 1451 Fragments arranged by size: 1451 1049 936 635 563 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3771 4169 4443 4590 5001 3771 Size: 398 274 147 411 4108 Fragments arranged by size: 4108 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2302 2332 1178 Size: 30 165 30 899 30 4184 Fragments arranged by size: 4184 899 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5338 BseYI C'CCAG_C Cuts at: 2313 4541 5037 2313 Size: 2228 496 2614 Fragments arranged by size: 2614 2228 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 1163 Size: 573 456 4309 Fragments arranged by size: 4309 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2788 3549 4577 1540 Size: 275 186 787 761 1028 2301 Fragments arranged by size: 2301 1028 787 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2280 2457 3399 3484 4555 2061 Size: 219 177 942 85 1071 2844 Fragments arranged by size: 2844 1071 942 219 177 85 BsmI GAATG_Cn' Cuts at: 2849 2942 2849 Size: 93 5245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2280 2457 2657 3399 3484 4555 Size: 399 271 219 177 200 742 85 1071 Cuts at: 4555 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 271 219 200 177 85 BspEI T'CCGG_A Cuts at: 5001 5001 Size: 5338 BspHI T'CATG_A Cuts at: 3227 4957 3227 Size: 1730 3608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2693 844 Size: 1849 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3225 1394 Size: 429 1402 3507 Fragments arranged by size: 3507 1402 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3833 4007 65 Size: 1183 2585 174 1396 Fragments arranged by size: 2585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2155 3982 4169 1700 Size: 110 345 1827 187 2869 Fragments arranged by size: 2869 1827 345 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5338 BssHII G'CGCG_C Cuts at: 1925 2620 1925 Size: 695 4643 BssSI C'ACGA_G Cuts at: 2458 3398 4410 2458 Size: 940 1012 3386 Fragments arranged by size: 3386 1012 940 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5338 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5338 BstYI r'GATC_y Cuts at: 1972 2490 2517 2668 3035 3420 3437 4889 4975 Size: 518 27 151 367 385 17 1452 86 Cuts at: 4975 4987 5333 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 518 385 367 346 151 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 513 Size: 552 1053 3733 Fragments arranged by size: 3733 1053 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2418 487 Size: 683 56 1192 3407 Fragments arranged by size: 3407 1192 683 56 BtsI GCAGTG_nn' Cuts at: 2209 2321 2854 3648 3668 2209 Size: 112 533 794 20 3879 Fragments arranged by size: 3879 794 533 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5303 DraI TTT'AAA Cuts at: 2595 2642 2595 Size: 47 5291 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 809 Size: 507 3029 1802 Fragments arranged by size: 3029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2049 2785 Size: 54 1425 50 275 18 208 11 736 Cuts at: 2785 3674 5303 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 736 275 208 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2785 1537 Size: 275 973 4090 Fragments arranged by size: 4090 973 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 3268 1353 Size: 1257 658 3423 Fragments arranged by size: 3423 1257 658 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2540 3925 4299 4445 387 Size: 1064 138 951 1385 374 146 1280 Fragments arranged by size: 1385 1280 1064 951 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2249 2408 3401 3998 4785 5053 Size: 393 461 156 159 993 597 787 268 Cuts at: 5053 1239 Size: 1524 Fragments arranged by size: 1524 993 787 597 461 393 268 159 156 EcoRI G'AATT_C Cuts at: 2647 3025 2647 Size: 378 4960 FspI TGC'GCA Cuts at: 3844 3844 Size: 5338 HaeII r_GCGC'y Cuts at: 1678 4485 1678 Size: 2807 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2013 2045 3979 4011 4053 Size: 32 135 32 441 32 1934 32 42 Cuts at: 4053 4085 1373 Size: 32 2626 Fragments arranged by size: 2626 1934 441 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2682 2928 669 Size: 988 281 744 246 3079 Fragments arranged by size: 3079 988 744 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5338 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KpnI G_GTAC'C Cuts at: 2663 2663 Size: 5338 MfeI C'AATT_G Cuts at: 2937 2937 Size: 5338 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 4452 4636 1305 Size: 1260 1887 184 2007 Fragments arranged by size: 2007 1887 1260 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2040 10 Size: 54 1425 343 208 3308 Fragments arranged by size: 3308 1425 343 208 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 3296 3655 3814 518 Size: 648 1381 749 359 159 2042 Fragments arranged by size: 2042 1381 749 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2368 2754 3431 4579 4824 1606 Size: 762 386 677 1148 245 2120 Fragments arranged by size: 2120 1148 762 677 386 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4786 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2785 2785 Size: 5338 NsiI A_TGCA'T Cuts at: 2550 2550 Size: 5338 NspI r_CATG'y Cuts at: 1132 1229 2548 2696 4241 1132 Size: 97 1319 148 1545 2229 Fragments arranged by size: 2229 1545 1319 148 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 1108 Size: 939 4399 PfoI T'CCnGG_A Cuts at: 2070 2196 2226 2412 2070 Size: 126 30 186 4996 Fragments arranged by size: 4996 186 126 30 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 4920 4952 3407 Size: 32 1481 32 3793 Fragments arranged by size: 3793 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5338 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PstI C_TGCA'G Cuts at: 830 2690 830 Size: 1860 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 PvuII CAG'CTG Cuts at: 2368 2368 Size: 5338 SalI G'TCGA_C Cuts at: 1936 2680 1936 Size: 744 4594 SapI GCTCTTCn'nnn_ Cuts at: 2610 2610 Size: 5338 SbfI CC_TGCA'GG Cuts at: 2690 2690 Size: 5338 ScaI AGT'ACT Cuts at: 3586 5298 3586 Size: 1712 3626 SfcI C'TryA_G Cuts at: 826 1045 2686 3821 4502 4693 826 Size: 219 1641 1135 681 191 1471 Fragments arranged by size: 1641 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2665 1872 Size: 793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3442 4343 4605 820 Size: 197 926 1499 901 262 1553 Fragments arranged by size: 1553 1499 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2696 1132 Size: 1564 3774 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2777 513 Size: 552 1712 3074 Fragments arranged by size: 3074 1712 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 3365 3382 3535 3720 4165 325 Size: 1255 747 1038 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1038 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3584 5296 Size: 274 80 33 51 1443 168 1439 1712 Cuts at: 5296 96 Size: 138 Fragments arranged by size: 1712 1443 1439 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2302 2334 5298 5330 2302 Size: 32 2964 32 2310 Fragments arranged by size: 2964 2310 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5338 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2479 2479 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5338 XmaI C'CCGG_G Cuts at: 1870 2663 1870 Size: 793 4545 XmnI GAAnn'nnTTC Cuts at: 3467 3467 Size: 5338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2583 3146 276 Size: 53 83 186 936 1049 563 2468 Fragments arranged by size: 2468 1049 936 563 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI