Restriction map for pFN21AE9658
 (Circular) (Six-base) MAPSORT of: pFN21AE9658.seq  Check: 8873  from: 1  to: 5338



pFN21AE9658  5338 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:14  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2585    3148     278 
   Size:         53      83     186     936    1049     563    2468

  Fragments arranged by size: 

                2468    1049     936     563     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2112    2681    1937 
   Size:        175     569    4594

  Fragments arranged by size: 

                4594     569     175
 
Acc65I G'GTAC_C

Cuts at:   2659    2659 
   Size:       5338
 
AclI AA'CG_TT

Cuts at:   1563    3465    3838    1563 
   Size:       1902     373    3063

  Fragments arranged by size: 

                3063    1902     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2408    3401    4785    5053    1632 
   Size:        776     993    1384     268    1917

  Fragments arranged by size: 

                1917    1384     993     776     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5141
 
AflIII A'CryG_T

Cuts at:   1203    4237    5214    1203 
   Size:       3034     977    1327

  Fragments arranged by size: 

                3034    1327     977
 
AgeI A'CCGG_T

Cuts at:   4169    4169 
   Size:       5338
 
AhdI GACnn_n'nnGTC

Cuts at:   4067    4067 
   Size:       5338
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5338
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5338
 
AlwNI CAG_nnn'CTG

Cuts at:   3081    4653    3081 
   Size:       1572    3766
 
ApaLI G'TGCA_C

Cuts at:   2453    3395    4551    2453 
   Size:        942    1156    3240

  Fragments arranged by size: 

                3240    1156     942
 
ApoI r'AATT_y

Cuts at:   1493    2294    2647    2873    3025    5134    1493 
   Size:        801     353     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     801     353     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3892     160 
   Size:        872    2860    1606

  Fragments arranged by size: 

                2860    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5338
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2663    1870 
   Size:         73     720    4545

  Fragments arranged by size: 

                4545     720      73
 
BamHI G'GATC_C

Cuts at:   2517    2668    3035    2517 
   Size:        151     367    4820

  Fragments arranged by size: 

                4820     367     151
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2659    4114     618 
   Size:        325     217     627     872    1455    1842

  Fragments arranged by size: 

                1842    1455     872     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2657    1391 
   Size:       1266    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4367
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3529    3563    1903 
   Size:         34    1592      34    3678

  Fragments arranged by size: 

                3678    1592      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3230    4440    3230 
   Size:       1210    4128
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5338
 
BfrBI ATG'CAT

Cuts at:   2548    2548 
   Size:       5338
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3949     136 
   Size:        107     122      71     631     605    2277    1525

  Fragments arranged by size: 

                2277    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5333    5333 
   Size:       5338
 
BlpI GC'TnA_GC

Cuts at:   2755    2755 
   Size:       5338
 
Bme1580I G_kGCm'C

Cuts at:   1790    2457    3399    4555    1790 
   Size:        667     942    1156    2573

  Fragments arranged by size: 

                2573    1156     942     667
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5338
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2279    3069    4027 
   Size:        416      53      83     399      24     862     790     958

Cuts at:   4027     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     862     790     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2093    2249    3998    1239 
   Size:        854     156    1749    2579

  Fragments arranged by size: 

                2579    1749     854     156
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3463    4328    4626    3463 
   Size:        865     298    4175

  Fragments arranged by size: 

                4175     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4001     882 
   Size:        617    2502    2219

  Fragments arranged by size: 

                2502    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2101    2611    3129     493 
   Size:        711     897     510     518    2702

  Fragments arranged by size: 

                2702     897     711     518     510
 
BsaBI GATnn'nnATC

Cuts at:   5332    5332 
   Size:       5338
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2582    3145    3527    4162 
   Size:         53      83     186     936    1049     563     382     635

Cuts at:   4162     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1049     936     635     563     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3771    4169    4443    4590    5001    3771 
   Size:        398     274     147     411    4108

  Fragments arranged by size: 

                4108     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2302    2332    1178 
   Size:         30     165      30     899      30    4184

  Fragments arranged by size: 

                4184     899     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5338
 
BseYI C'CCAG_C

Cuts at:   2313    4541    5037    2313 
   Size:       2228     496    2614

  Fragments arranged by size: 

                2614    2228     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2192    1163 
   Size:        573     456    4309

  Fragments arranged by size: 

                4309     573     456
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2788    3549    4577    1540 
   Size:        275     186     787     761    1028    2301

  Fragments arranged by size: 

                2301    1028     787     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2061    2280    2457    3399    3484    4555    2061 
   Size:        219     177     942      85    1071    2844

  Fragments arranged by size: 

                2844    1071     942     219     177      85
 
BsmI GAATG_Cn'

Cuts at:   2849    2942    2849 
   Size:         93    5245
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2061    2280    2457    2657    3399    3484    4555 
   Size:        399     271     219     177     200     742      85    1071

Cuts at:   4555    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     399     271     219     200     177
                  85
 
BspEI T'CCGG_A

Cuts at:   5001    5001 
   Size:       5338
 
BspHI T'CATG_A

Cuts at:   3227    4957    3227 
   Size:       1730    3608
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2693     844 
   Size:       1849    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3225    1394 
   Size:        429    1402    3507

  Fragments arranged by size: 

                3507    1402     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3833    4007      65 
   Size:       1183    2585     174    1396

  Fragments arranged by size: 

                2585    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2155    3982    4169    1700 
   Size:        110     345    1827     187    2869

  Fragments arranged by size: 

                2869    1827     345     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5338
 
BssHII G'CGCG_C

Cuts at:   1925    2620    1925 
   Size:        695    4643
 
BssSI C'ACGA_G

Cuts at:   2458    3398    4410    2458 
   Size:        940    1012    3386

  Fragments arranged by size: 

                3386    1012     940
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5338
 
BstBI TT'CG_AA

Cuts at:   4142    4142 
   Size:       5338
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5338
 
BstYI r'GATC_y

Cuts at:   1972    2490    2517    2668    3035    3420    3437    4889    4975 
   Size:        518      27     151     367     385      17    1452      86

Cuts at:   4975    4987    5333    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     518     385     367     346     151      86
                  27      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2118     513 
   Size:        552    1053    3733

  Fragments arranged by size: 

                3733    1053     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2418     487 
   Size:        683      56    1192    3407

  Fragments arranged by size: 

                3407    1192     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2209    2321    2854    3648    3668    2209 
   Size:        112     533     794      20    3879

  Fragments arranged by size: 

                3879     794     533     112      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5338
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5303
 
DraI TTT'AAA

Cuts at:   2595    2642    2595 
   Size:         47    5291
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4345     809 
   Size:        507    3029    1802

  Fragments arranged by size: 

                3029    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2038    2049    2785 
   Size:         54    1425      50     275      18     208      11     736

Cuts at:   2785    3674    5303       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     736     275     208      54      50
                  43      18      11
 
EagI C'GGCC_G

Cuts at:   1537    1812    2785    1537 
   Size:        275     973    4090

  Fragments arranged by size: 

                4090     973     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2610    3268    1353 
   Size:       1257     658    3423

  Fragments arranged by size: 

                3423    1257     658
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2540    3925    4299    4445     387 
   Size:       1064     138     951    1385     374     146    1280

  Fragments arranged by size: 

                1385    1280    1064     951     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2093    2249    2408    3401    3998    4785    5053 
   Size:        393     461     156     159     993     597     787     268

Cuts at:   5053    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     993     787     597     461     393     268     159
                 156
 
EcoRI G'AATT_C

Cuts at:   2647    3025    2647 
   Size:        378    4960
 
FspI TGC'GCA

Cuts at:   3844    3844 
   Size:       5338
 
HaeII r_GCGC'y

Cuts at:   1678    4485    1678 
   Size:       2807    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2013    2045    3979    4011    4053 
   Size:         32     135      32     441      32    1934      32      42

Cuts at:   4053    4085    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1934     441     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2682    2928     669 
   Size:        988     281     744     246    3079

  Fragments arranged by size: 

                3079     988     744     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5338
 
HpaI GTT'AAC

Cuts at:   2928    2928 
   Size:       5338
 
KpnI G_GTAC'C

Cuts at:   2663    2663 
   Size:       5338
 
MfeI C'AATT_G

Cuts at:   2937    2937 
   Size:       5338
 
MluI A'CGCG_T

Cuts at:   5214    5214 
   Size:       5338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2565    4452    4636    1305 
   Size:       1260    1887     184    2007

  Fragments arranged by size: 

                2007    1887    1260     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2040      10 
   Size:         54    1425     343     208    3308

  Fragments arranged by size: 

                3308    1425     343     208      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2547    3296    3655    3814     518 
   Size:        648    1381     749     359     159    2042

  Fragments arranged by size: 

                2042    1381     749     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2368    2754    3431    4579    4824    1606 
   Size:        762     386     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     762     677     386     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4786
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5338
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5338
 
NotI GC'GGCC_GC

Cuts at:   2785    2785 
   Size:       5338
 
NsiI A_TGCA'T

Cuts at:   2550    2550 
   Size:       5338
 
NspI r_CATG'y

Cuts at:   1132    1229    2548    2696    4241    1132 
   Size:         97    1319     148    1545    2229

  Fragments arranged by size: 

                2229    1545    1319     148      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5338
 
PciI A'CATG_T

Cuts at:   4237    4237 
   Size:       5338
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2047    1108 
   Size:        939    4399
 
PfoI T'CCnGG_A

Cuts at:   2070    2196    2226    2412    2070 
   Size:        126      30     186    4996

  Fragments arranged by size: 

                4996     186     126      30
 
PmeI GTTT'AAAC

Cuts at:   2642    2642 
   Size:       5338
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3407    3439    4920    4952    3407 
   Size:         32    1481      32    3793

  Fragments arranged by size: 

                3793    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5338
 
PsiI TTA'TAA

Cuts at:   2908    2908 
   Size:       5338
 
PstI C_TGCA'G

Cuts at:    830    2690     830 
   Size:       1860    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5338
 
PvuII CAG'CTG

Cuts at:   2368    2368 
   Size:       5338
 
SalI G'TCGA_C

Cuts at:   1936    2680    1936 
   Size:        744    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2610    2610 
   Size:       5338
 
SbfI CC_TGCA'GG

Cuts at:   2690    2690 
   Size:       5338
 
ScaI AGT'ACT

Cuts at:   3586    5298    3586 
   Size:       1712    3626
 
SfcI C'TryA_G

Cuts at:    826    1045    2686    3821    4502    4693     826 
   Size:        219    1641    1135     681     191    1471

  Fragments arranged by size: 

                1641    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5338
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5338
 
SmaI CCC'GGG

Cuts at:   1872    2665    1872 
   Size:        793    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3442    4343    4605     820 
   Size:        197     926    1499     901     262    1553

  Fragments arranged by size: 

                1553    1499     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3129     493 
   Size:       2636    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5338
 
SphI G_CATG'C

Cuts at:   1132    2696    1132 
   Size:       1564    3774
 
SspI AAT'ATT

Cuts at:      5      52    3262       5 
   Size:         47    3210    2081

  Fragments arranged by size: 

                3210    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5338
 
StyI C'CwwG_G

Cuts at:    513    1065    2777     513 
   Size:        552    1712    3074

  Fragments arranged by size: 

                3074    1712     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2327    3365    3382    3535    3720    4165     325 
   Size:       1255     747    1038      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1038     747     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2145    3584    5296 
   Size:        274      80      33      51    1443     168    1439    1712

Cuts at:   5296      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1439     274     168     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2302    2334    5298    5330    2302 
   Size:         32    2964      32    2310

  Fragments arranged by size: 

                2964    2310      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5338
 
XbaI T'CTAG_A

Cuts at:   2674    2674 
   Size:       5338
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2479    2479 
   Size:       5338
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5338
 
XmaI C'CCGG_G

Cuts at:   1870    2663    1870 
   Size:        793    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3467    3467 
   Size:       5338
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2583    3146     276 
   Size:         53      83     186     936    1049     563    2468

  Fragments arranged by size: 

                2468    1049     936     563     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     PacI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI
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