################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BalI BbeI BbrPI BcgI BciVI BfrI BglI BglII BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 871 GTCTAC GT^[AC][GT]AC AccIII 1414 TCCGGA T^CCGGA AfaI 834 GTAC GT^AC AflIII 954 ACATGT A^C[GA][CT]GT AvaI 1046 CTCGGG C^[CT]CG[GA]G AvrII 21 CCTAGG C^CTAGG BbvCI 993 CCTCAGC CCTCAGC(-5/-2) BlnI 21 CCTAGG C^CTAGG BlpI 369 GCTGAGC GC^T[ACGT]AGC Bpu1102I 369 GCTGAGC GC^T[ACGT]AGC BsaI 1051 GGTCTC GGTCTC(1/5) BsaWI 1414 TCCGGA [AT]^CCGG[AT] BseAI 1414 TCCGGA T^CCGGA BseRI 218 CTCCTC GAGGAG(10/8) BsoBI 1046 CTCGGG C^[CT]CG[GA]G BspEI 1414 TCCGGA T^CCGGA BspHI 1031 TCATGA T^CATGA BspLU11I 954 ACATGT A^CATGT BsrBI 1043 CCGCTC CCGCTC(-3/-3) BsrDI 1238 GCAATG GCAATG(2/0) BsrFI 887 GCCGGC [GA]^CCGG[CT] BssSI 1114 CACGAG CACGAG(-5/-1) BstEII 597 GGTAACC G^GT[ACGT]ACC BstPI 597 GGTAACC G^GT[ACGT]ACC BstXI 1524 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 369 GCTGAGC GC^T[ACGT]AGC Cfr10I 887 GCCGGC [GA]^CCGG[CT] EcoNI 1630 CCTGTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 597 GGTAACC G^GT[ACGT]ACC FauI 1014 GCGGG CCCGC(4/6) HaeII 1000 AGCGCC [GA]GCGC^[CT] HgaI 87 GACGC GACGC(5/10) MroI 1414 TCCGGA T^CCGGA NaeI 887 GCCGGC GCC^GGC NcoI 1405 CCATGG C^CATGG NgoMIV 887 GCCGGC G^CCGGC NspI 954 ACATGT [GA]CATG^[CT] PstI 913 CTGCAG CTGCA^G RcaI 1031 TCATGA T^CATGA RsaI 834 GTAC GT^AC Tsp509I 1206 AATT ^AATT XbaI 822 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BanI 655 GGCACC G^G[CT][GA]CC 850 GGTGCC BbsI 1109 GAAGAC GAAGAC(2/6) 1671 GAAGAC BclI 341 TGATCA T^GATCA 1460 TGATCA BpuAI 1109 GAAGAC GAAGAC(2/6) 1671 GAAGAC BspMI 1304 ACCTGC ACCTGC(4/8) 564 GCAGGT CfoI 732 GCGC GCG^C 1001 GCGC DraII 429 AGGACCT [GA]G^G[ACGT]CC[CT] 454 AGGTCCC DraIII 289 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 418 CACTATGTG EcoO109I 429 AGGACCT [GA]G^G[ACGT]CC[CT] 454 AGGTCCC FbaI 341 TGATCA T^GATCA 1460 TGATCA HhaI 732 GCGC GCG^C 1001 GCGC HinP1I 732 GCGC G^CGC 1001 GCGC HincII 1018 GTCAAC GT[CT]^[GA]AC 1210 GTTGAC HindII 1018 GTCAAC GT[CT]^[GA]AC 1210 GTTGAC MaeII 552 ACGT A^CGT 644 ACGT MslI 415 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1155 CACCACAGTG MspA1I 79 CAGCGG C[AC]G^C[GT]G 1012 CAGCGG PpuMI 429 AGGACCT [GA]G^G[AT]CC[CT] 454 AGGTCCC SacI 1027 GAGCTC GAGCT^C 1290 GAGCTC SapI 133 GAAGAGC GCTCTTC(1/4) 1062 GCTCTTC StuI 482 AGGCCT AGG^CCT 582 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AluI AlwI AlwNI AocI AspHI AvaII BamHI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI Eco81I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI Van91I XhoII