(Circular) (Six-base) MAPSORT of: pFN21AE4523.seq Check: 3294 from: 1 to: 6394 pFN21AE4523 6394 bp With 182 enzymes: SgfI * March 28, 2012 14:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2560 3318 2560 Size: 758 5636 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4204 278 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2877 3737 1937 Size: 940 860 4594 Fragments arranged by size: 4594 940 860 Acc65I G'GTAC_C Cuts at: 3715 3715 Size: 6394 AclI AA'CG_TT Cuts at: 1563 4521 4894 1563 Size: 2958 373 3063 Fragments arranged by size: 3063 2958 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3223 3695 4457 5841 6109 1632 Size: 1591 472 762 1384 268 1917 Fragments arranged by size: 1917 1591 1384 762 472 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6197 AflIII A'CryG_T Cuts at: 1203 2959 5293 6270 1203 Size: 1756 2334 977 1327 Fragments arranged by size: 2334 1756 1327 977 AgeI A'CCGG_T Cuts at: 5225 5225 Size: 6394 AhdI GACnn_n'nnGTC Cuts at: 5123 5123 Size: 6394 AleI CACnn'nnGTG Cuts at: 1166 3164 1166 Size: 1998 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3416 1184 Size: 2232 4162 AlwNI CAG_nnn'CTG Cuts at: 2582 2695 2842 4137 5709 2582 Size: 113 147 1295 1572 3267 Fragments arranged by size: 3267 1572 1295 147 113 ApaLI G'TGCA_C Cuts at: 4451 5607 4451 Size: 1156 5238 ApoI r'AATT_y Cuts at: 1493 3703 3929 4081 6190 1493 Size: 2210 226 152 2109 1697 Fragments arranged by size: 2210 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4948 160 Size: 872 3916 1606 Fragments arranged by size: 3916 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 AvaI C'yCGr_G Cuts at: 1870 1943 3051 3719 1870 Size: 73 1108 668 4545 Fragments arranged by size: 4545 1108 668 73 AvrII C'CTAG_G Cuts at: 2026 2026 Size: 6394 BamHI G'GATC_C Cuts at: 2212 3453 3480 3631 3724 4091 2212 Size: 1241 27 151 93 367 4515 Fragments arranged by size: 4515 1241 367 151 93 27 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2660 2855 3715 5170 618 Size: 325 217 627 873 195 860 1455 1842 Fragments arranged by size: 1842 1455 873 860 627 325 217 195 BanII G_rGCy'C Cuts at: 1391 2034 2079 2329 2657 2913 3036 3299 3713 Size: 643 45 250 328 256 123 263 414 Cuts at: 3713 1391 Size: 4072 Fragments arranged by size: 4072 643 414 328 263 256 250 123 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3121 3683 928 Size: 971 1222 562 3639 Fragments arranged by size: 3639 1222 971 562 BbvCI CC'TCA_GC Cuts at: 2999 2999 Size: 6394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4585 4619 1903 Size: 34 2648 34 3678 Fragments arranged by size: 3678 2648 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4286 5496 4286 Size: 1210 5184 BclI T'GATC_A Cuts at: 1548 2346 3465 1548 Size: 798 1119 4477 Fragments arranged by size: 4477 1119 798 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5005 136 Size: 107 122 71 631 605 3333 1525 Fragments arranged by size: 3333 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6389 6389 Size: 6394 BlpI GC'TnA_GC Cuts at: 2375 3811 2375 Size: 1436 4958 Bme1580I G_kGCm'C Cuts at: 1790 2689 3405 4455 5611 1790 Size: 899 716 1050 1156 2573 Fragments arranged by size: 2573 1156 1050 899 716 BmgBI CAC'GTC Cuts at: 1318 2650 1318 Size: 1332 5062 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2683 3391 4125 Size: 416 53 83 399 24 1266 708 734 Cuts at: 4125 5083 442 Size: 958 1753 Fragments arranged by size: 1753 1266 958 734 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2717 3130 3423 5054 1239 Size: 1478 413 293 1631 2579 Fragments arranged by size: 2579 1631 1478 413 293 Bpu10I CC'TnA_GC Cuts at: 2562 2999 3293 2562 Size: 437 294 5663 Fragments arranged by size: 5663 437 294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4519 5384 5682 4519 Size: 865 298 5231 Fragments arranged by size: 5231 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3062 5057 882 Size: 617 1563 1995 2219 Fragments arranged by size: 2219 1995 1563 617 BsaAI yAC'GTr Cuts at: 493 1204 4185 493 Size: 711 2981 2702 Fragments arranged by size: 2981 2702 711 BsaBI GATnn'nnATC Cuts at: 6388 6388 Size: 6394 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4201 4583 5218 275 Size: 53 83 186 936 2668 382 635 1451 Fragments arranged by size: 2668 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3419 4827 5225 5499 5646 6057 3419 Size: 1408 398 274 147 411 3756 Fragments arranged by size: 3756 1408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2274 2304 1178 Size: 30 165 30 871 30 5268 Fragments arranged by size: 5268 871 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2214 1186 Size: 1028 5366 BseYI C'CCAG_C Cuts at: 2106 2216 3627 3645 3664 5597 6093 2106 Size: 110 1411 18 19 1933 496 2407 Fragments arranged by size: 2407 1933 1411 496 110 19 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2507 2855 3348 1163 Size: 573 483 288 348 493 4209 Fragments arranged by size: 4209 573 493 483 348 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3844 4605 5633 1540 Size: 275 186 1843 761 1028 2301 Fragments arranged by size: 2301 1843 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3036 3299 3477 4455 4540 5611 3036 Size: 263 178 978 85 1071 3819 Fragments arranged by size: 3819 1071 978 263 178 85 BsmI GAATG_Cn' Cuts at: 3905 3998 3905 Size: 93 6301 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2079 2329 2657 2689 2913 3036 Size: 399 244 45 250 328 32 224 123 Cuts at: 3036 3299 3405 3477 3713 4455 4540 5611 1391 Size: 263 106 72 236 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 263 250 244 236 224 123 106 85 72 45 32 BspEI T'CCGG_A Cuts at: 3419 6057 3419 Size: 2638 3756 BspHI T'CATG_A Cuts at: 3036 4283 6013 3036 Size: 1247 1730 3417 Fragments arranged by size: 3417 1730 1247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 3318 3749 844 Size: 1716 758 431 3489 Fragments arranged by size: 3489 1716 758 431 BsrBI CCG'CTC Cuts at: 1394 1823 3050 4281 1394 Size: 429 1227 1231 3507 Fragments arranged by size: 3507 1231 1227 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3250 4889 5063 65 Size: 1183 2002 1639 174 1396 Fragments arranged by size: 2002 1639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2892 5038 5225 1700 Size: 110 1082 2146 187 2869 Fragments arranged by size: 2869 2146 1082 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6394 BssSI C'ACGA_G Cuts at: 3119 4454 5466 3119 Size: 1335 1012 4047 Fragments arranged by size: 4047 1335 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6394 BstBI TT'CG_AA Cuts at: 5198 5198 Size: 6394 BstEII G'GTnAC_C Cuts at: 1184 2602 1184 Size: 1418 4976 BstXI CCAn_nnnn'nTGG Cuts at: 3536 3536 Size: 6394 BstYI r'GATC_y Cuts at: 1972 2212 3453 3480 3631 3724 4091 4476 4493 Size: 240 1241 27 151 93 367 385 17 Cuts at: 4493 5945 6031 6043 6389 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1241 385 367 346 240 151 93 86 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2133 2549 3076 2133 Size: 416 527 5451 Fragments arranged by size: 5451 527 416 BtgI C'CryG_G Cuts at: 513 1065 2004 3254 3410 3562 513 Size: 552 939 1250 156 152 3345 Fragments arranged by size: 3345 1250 939 552 156 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5655 Fragments arranged by size: 5655 683 56 BtsI GCAGTG_nn' Cuts at: 2927 3910 4704 4724 2927 Size: 983 794 20 4597 Fragments arranged by size: 4597 983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2472 2507 3319 3354 529 Size: 35 1908 35 812 35 3569 Fragments arranged by size: 3569 1908 812 35 35 35 DraI TTT'AAA Cuts at: 3698 3698 Size: 6394 DraIII CAC_nnn'GTG Cuts at: 2299 2428 2299 Size: 129 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5401 809 Size: 507 4085 1802 Fragments arranged by size: 4085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2890 3340 3490 Size: 54 1425 50 275 18 1060 450 150 Cuts at: 3490 3841 4730 6359 8 Size: 351 889 1629 43 Fragments arranged by size: 1629 1425 1060 889 450 351 275 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3841 1537 Size: 275 2029 4090 Fragments arranged by size: 4090 2029 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2747 3074 3585 4324 1353 Size: 780 614 327 511 739 3423 Fragments arranged by size: 3423 780 739 614 511 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3116 4981 5355 5501 387 Size: 1064 138 1527 1865 374 146 1280 Fragments arranged by size: 1865 1527 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3034 3297 3034 Size: 263 6131 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2717 3130 3223 3423 3695 4457 5054 Size: 393 1085 413 93 200 272 762 597 Cuts at: 5054 5841 6109 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1085 787 762 597 413 393 272 268 200 93 EcoNI CCTnn'n_nnAGG Cuts at: 3639 3639 Size: 6394 EcoO109I rG'GnC_Cy Cuts at: 2435 2460 2435 Size: 25 6369 EcoRI G'AATT_C Cuts at: 3703 4081 3703 Size: 378 6016 FspI TGC'GCA Cuts at: 4900 4900 Size: 6394 HaeII r_GCGC'y Cuts at: 1678 3009 5541 1678 Size: 1331 2532 2531 Fragments arranged by size: 2532 2531 1331 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2542 2574 2639 Size: 32 135 32 632 32 306 32 65 Cuts at: 2639 2671 2978 3010 5035 5067 5109 5141 1373 Size: 32 307 32 2025 32 42 32 2626 Fragments arranged by size: 2626 2025 632 307 306 135 65 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3025 3217 3738 3984 669 Size: 988 281 1087 192 521 246 3079 Fragments arranged by size: 3079 1087 988 521 281 246 192 HindIII A'AGCT_T Cuts at: 748 748 Size: 6394 HpaI GTT'AAC Cuts at: 3984 3984 Size: 6394 KpnI G_GTAC'C Cuts at: 3719 3719 Size: 6394 MfeI C'AATT_G Cuts at: 3993 3993 Size: 6394 MluI A'CGCG_T Cuts at: 6270 6270 Size: 6394 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2190 2407 2767 5508 5692 1305 Size: 885 217 360 2741 184 2007 Fragments arranged by size: 2741 2007 885 360 217 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4572 Fragments arranged by size: 4572 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2424 3164 4352 4711 4870 518 Size: 648 1258 740 1188 359 159 2042 Fragments arranged by size: 2042 1258 1188 740 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2086 3019 3810 4487 5635 5880 1606 Size: 480 933 791 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 791 677 480 245 NaeI GCC'GGC Cuts at: 2894 2894 Size: 6394 NcoI C'CATG_G Cuts at: 513 1065 2004 3410 513 Size: 552 939 1406 3497 Fragments arranged by size: 3497 1406 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6394 NgoMIV G'CCGG_C Cuts at: 2892 2892 Size: 6394 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6394 NotI GC'GGCC_GC Cuts at: 3841 3841 Size: 6394 NspI r_CATG'y Cuts at: 1132 1229 2963 3752 5297 1132 Size: 97 1734 789 1545 2229 Fragments arranged by size: 2229 1734 1545 789 97 PasI CC'CwG_GG Cuts at: 1348 2116 2405 1348 Size: 768 289 5337 Fragments arranged by size: 5337 768 289 PciI A'CATG_T Cuts at: 2959 5293 2959 Size: 2334 4060 PflMI CCAn_nnn'nTGG Cuts at: 1108 2695 3411 3446 1108 Size: 1587 716 35 4056 Fragments arranged by size: 4056 1587 716 35 PfoI T'CCnGG_A Cuts at: 2694 3183 2694 Size: 489 5905 PmeI GTTT'AAAC Cuts at: 3698 3698 Size: 6394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3180 3212 4463 4495 5976 6008 3180 Size: 32 1251 32 1481 32 3566 Fragments arranged by size: 3566 1481 1251 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2435 2460 2435 Size: 25 6369 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6394 PsiI TTA'TAA Cuts at: 3964 3964 Size: 6394 PstI C_TGCA'G Cuts at: 830 2922 3746 830 Size: 2092 824 3478 Fragments arranged by size: 3478 2092 824 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6394 SacI G_AGCT'C Cuts at: 3036 3299 3036 Size: 263 6131 SalI G'TCGA_C Cuts at: 1936 3736 1936 Size: 1800 4594 SapI GCTCTTCn'nnn_ Cuts at: 2133 3074 2133 Size: 941 5453 SbfI CC_TGCA'GG Cuts at: 3746 3746 Size: 6394 ScaI AGT'ACT Cuts at: 4642 6354 4642 Size: 1712 4682 SfcI C'TryA_G Cuts at: 826 1045 2583 2878 2918 3742 4877 5558 5749 Size: 219 1538 295 40 824 1135 681 191 Cuts at: 5749 826 Size: 1471 Fragments arranged by size: 1538 1471 1135 824 681 295 219 191 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6394 SmaI CCC'GGG Cuts at: 1872 3721 1872 Size: 1849 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4498 5399 5661 820 Size: 197 926 2555 901 262 1553 Fragments arranged by size: 2555 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4185 493 Size: 3692 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6394 SphI G_CATG'C Cuts at: 1132 3752 1132 Size: 2620 3774 SspI AAT'ATT Cuts at: 5 52 4318 5 Size: 47 4266 2081 Fragments arranged by size: 4266 2081 47 StuI AGG'CCT Cuts at: 1517 2489 2589 1517 Size: 972 100 5322 Fragments arranged by size: 5322 972 100 StyI C'CwwG_G Cuts at: 513 1065 2004 2026 2490 3410 3518 3833 513 Size: 552 939 22 464 920 108 315 3074 Fragments arranged by size: 3074 939 920 552 464 315 108 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2098 3373 3403 3670 4421 4438 4591 Size: 1255 518 1275 30 267 751 17 153 Cuts at: 4591 4776 5221 325 Size: 185 445 1498 Fragments arranged by size: 1498 1275 1255 751 518 445 267 185 153 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4640 6352 96 Size: 274 80 33 51 1443 2663 1712 138 Fragments arranged by size: 2663 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 3472 3504 3645 3677 6354 6386 2274 Size: 32 1166 32 141 32 2677 32 2282 Fragments arranged by size: 2677 2282 1166 141 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6394 XbaI T'CTAG_A Cuts at: 2827 3730 2827 Size: 903 5491 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6394 XmaI C'CCGG_G Cuts at: 1870 3719 1870 Size: 1849 4545 XmnI GAAnn'nnTTC Cuts at: 4523 4523 Size: 6394 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4202 276 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI