(Linear) (Six-base) MAPSORT of: KIEE4523.seq Check: 3601 from: 1 to: 1689 KIEE4523.orf With 182 enzymes: SgfI * March 22, 2012 18:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 555 1313 1689 Size: 555 758 376 Fragments arranged by size: 758 555 376 AccI GT'mk_AC Cuts at: 0 872 1689 Size: 872 817 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1218 1689 Size: 1218 471 AflIII A'CryG_T Cuts at: 0 954 1689 Size: 954 735 AleI CACnn'nnGTG Cuts at: 0 1159 1689 Size: 1159 530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1411 1689 Size: 1411 278 AlwNI CAG_nnn'CTG Cuts at: 0 577 690 837 1689 Size: 577 113 147 852 Fragments arranged by size: 852 577 147 113 AvaI C'yCGr_G Cuts at: 0 1046 1689 Size: 1046 643 AvrII C'CTAG_G Cuts at: 0 21 1689 Size: 21 1668 BamHI G'GATC_C Cuts at: 0 207 1448 1475 1626 1689 Size: 207 1241 27 151 63 Fragments arranged by size: 1241 207 151 63 27 BanI G'GyrC_C Cuts at: 0 655 850 1689 Size: 655 195 839 Fragments arranged by size: 839 655 195 BanII G_rGCy'C Cuts at: 0 29 74 324 652 908 1031 1294 1689 Size: 29 45 250 328 256 123 263 395 Fragments arranged by size: 395 328 263 256 250 123 45 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 1116 1678 1689 Size: 1116 562 11 Fragments arranged by size: 1116 562 11 BbvCI CC'TCA_GC Cuts at: 0 994 1689 Size: 994 695 BclI T'GATC_A Cuts at: 0 341 1460 1689 Size: 341 1119 229 Fragments arranged by size: 1119 341 229 BlpI GC'TnA_GC Cuts at: 0 370 1689 Size: 370 1319 Bme1580I G_kGCm'C Cuts at: 0 684 1400 1689 Size: 684 716 289 Fragments arranged by size: 716 684 289 BmgBI CAC'GTC Cuts at: 0 645 1689 Size: 645 1044 BmrI ACTGGGnnnn_n' Cuts at: 0 678 1386 1689 Size: 678 708 303 Fragments arranged by size: 708 678 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 1125 1418 1689 Size: 712 413 293 271 Fragments arranged by size: 712 413 293 271 Bpu10I CC'TnA_GC Cuts at: 0 557 994 1288 1689 Size: 557 437 294 401 Fragments arranged by size: 557 437 401 294 BsaI GGTCTCn'nnnn_ Cuts at: 0 1057 1689 Size: 1057 632 BsaWI w'CCGG_w Cuts at: 0 1414 1689 Size: 1414 275 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 269 299 1689 Size: 269 30 1390 Fragments arranged by size: 1390 269 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 209 1689 Size: 209 1480 BseYI C'CCAG_C Cuts at: 0 101 211 1622 1640 1659 1689 Size: 101 110 1411 18 19 30 Fragments arranged by size: 1411 110 101 30 19 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 502 850 1343 1689 Size: 214 288 348 493 346 Fragments arranged by size: 493 348 346 288 214 BsiHKAI G_wGCw'C Cuts at: 0 1031 1294 1472 1689 Size: 1031 263 178 217 Fragments arranged by size: 1031 263 217 178 Bsp1286I G_dGCh'C Cuts at: 0 29 74 324 652 684 908 1031 1294 Size: 29 45 250 328 32 224 123 263 Cuts at: 1294 1400 1472 1689 Size: 106 72 217 Fragments arranged by size: 328 263 250 224 217 123 106 72 45 32 29 BspEI T'CCGG_A Cuts at: 0 1414 1689 Size: 1414 275 BspHI T'CATG_A Cuts at: 0 1031 1689 Size: 1031 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 555 1313 1689 Size: 555 758 376 Fragments arranged by size: 758 555 376 BsrBI CCG'CTC Cuts at: 0 1045 1689 Size: 1045 644 BsrDI GCAATG_nn' Cuts at: 0 1245 1689 Size: 1245 444 BsrFI r'CCGG_y Cuts at: 0 887 1689 Size: 887 802 BssSI C'ACGA_G Cuts at: 0 1114 1689 Size: 1114 575 BstEII G'GTnAC_C Cuts at: 0 597 1689 Size: 597 1092 BstXI CCAn_nnnn'nTGG Cuts at: 0 1531 1689 Size: 1531 158 BstYI r'GATC_y Cuts at: 0 207 1448 1475 1626 1689 Size: 207 1241 27 151 63 Fragments arranged by size: 1241 207 151 63 27 Bsu36I CC'TnA_GG Cuts at: 0 128 544 1071 1689 Size: 128 416 527 618 Fragments arranged by size: 618 527 416 128 BtgI C'CryG_G Cuts at: 0 1249 1405 1557 1689 Size: 1249 156 152 132 Fragments arranged by size: 1249 156 152 132 BtsI GCAGTG_nn' Cuts at: 0 922 1689 Size: 922 767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 467 502 1314 1349 1689 Size: 467 35 812 35 340 Fragments arranged by size: 812 467 340 35 35 DraIII CAC_nnn'GTG Cuts at: 0 294 423 1689 Size: 294 129 1266 Fragments arranged by size: 1266 294 129 EaeI y'GGCC_r Cuts at: 0 885 1335 1485 1689 Size: 885 450 150 204 Fragments arranged by size: 885 450 204 150 EarI CTCTTCn'nnn_ Cuts at: 0 128 742 1069 1580 1689 Size: 128 614 327 511 109 Fragments arranged by size: 614 511 327 128 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1111 1689 Size: 1111 578 EcoICRI GAG'CTC Cuts at: 0 1029 1292 1689 Size: 1029 263 397 Fragments arranged by size: 1029 397 263 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 1125 1218 1418 1689 Size: 712 413 93 200 271 Fragments arranged by size: 712 413 271 200 93 EcoNI CCTnn'n_nnAGG Cuts at: 0 1634 1689 Size: 1634 55 EcoO109I rG'GnC_Cy Cuts at: 0 430 455 1689 Size: 430 25 1234 Fragments arranged by size: 1234 430 25 HaeII r_GCGC'y Cuts at: 0 1004 1689 Size: 1004 685 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 199 231 537 569 634 666 973 1005 Size: 199 32 306 32 65 32 307 32 Cuts at: 1005 1689 Size: 684 Fragments arranged by size: 684 307 306 199 65 32 32 32 32 HincII GTy'rAC Cuts at: 0 1020 1212 1689 Size: 1020 192 477 Fragments arranged by size: 1020 477 192 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 185 402 762 1689 Size: 185 217 360 927 Fragments arranged by size: 927 360 217 185 MslI CAynn'nnrTG Cuts at: 0 419 1159 1689 Size: 419 740 530 Fragments arranged by size: 740 530 419 MspA1I CmG'CkG Cuts at: 0 81 1014 1689 Size: 81 933 675 Fragments arranged by size: 933 675 81 NaeI GCC'GGC Cuts at: 0 889 1689 Size: 889 800 NcoI C'CATG_G Cuts at: 0 1405 1689 Size: 1405 284 NgoMIV G'CCGG_C Cuts at: 0 887 1689 Size: 887 802 NspI r_CATG'y Cuts at: 0 958 1689 Size: 958 731 PasI CC'CwG_GG Cuts at: 0 111 400 1689 Size: 111 289 1289 Fragments arranged by size: 1289 289 111 PciI A'CATG_T Cuts at: 0 954 1689 Size: 954 735 PflMI CCAn_nnn'nTGG Cuts at: 0 690 1406 1441 1689 Size: 690 716 35 248 Fragments arranged by size: 716 690 248 35 PfoI T'CCnGG_A Cuts at: 0 689 1178 1689 Size: 689 489 511 Fragments arranged by size: 689 511 489 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1175 1207 1689 Size: 1175 32 482 Fragments arranged by size: 1175 482 32 PpuMI rG'GwC_Cy Cuts at: 0 430 455 1689 Size: 430 25 1234 Fragments arranged by size: 1234 430 25 PstI C_TGCA'G Cuts at: 0 917 1689 Size: 917 772 SacI G_AGCT'C Cuts at: 0 1031 1294 1689 Size: 1031 263 395 Fragments arranged by size: 1031 395 263 SapI GCTCTTCn'nnn_ Cuts at: 0 128 1069 1689 Size: 128 941 620 Fragments arranged by size: 941 620 128 SfcI C'TryA_G Cuts at: 0 578 873 913 1689 Size: 578 295 40 776 Fragments arranged by size: 776 578 295 40 StuI AGG'CCT Cuts at: 0 484 584 1689 Size: 484 100 1105 Fragments arranged by size: 1105 484 100 StyI C'CwwG_G Cuts at: 0 21 485 1405 1513 1689 Size: 21 464 920 108 176 Fragments arranged by size: 920 464 176 108 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 93 1368 1398 1665 1689 Size: 93 1275 30 267 24 Fragments arranged by size: 1275 267 93 30 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 269 301 1467 1499 1640 1672 1689 Size: 269 32 1166 32 141 32 17 Fragments arranged by size: 1166 269 141 32 32 32 17 XbaI T'CTAG_A Cuts at: 0 822 1689 Size: 822 867 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI AvaI AvrII BamHI BanI BanII BbsI BbvCI BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PstI SacI SapI SfcI StuI StyI TaqII TstI XbaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BbeI BcgI BciVI BfrBI BglI BglII BmtI BplI BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI ClaI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XcmI XhoI XmaI XmnI ZraI