Restriction map for pFN21AE4523
 (Linear) (Six-base) MAPSORT of: KIEE4523.seq  Check: 3601  from: 1  to: 1689



KIEE4523.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     555    1313    1689 
   Size:        555     758     376

  Fragments arranged by size: 

                 758     555     376
 
AccI GT'mk_AC

Cuts at:      0     872    1689 
   Size:        872     817
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1218    1689 
   Size:       1218     471
 
AflIII A'CryG_T

Cuts at:      0     954    1689 
   Size:        954     735
 
AleI CACnn'nnGTG

Cuts at:      0    1159    1689 
   Size:       1159     530
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1411    1689 
   Size:       1411     278
 
AlwNI CAG_nnn'CTG

Cuts at:      0     577     690     837    1689 
   Size:        577     113     147     852

  Fragments arranged by size: 

                 852     577     147     113
 
AvaI C'yCGr_G

Cuts at:      0    1046    1689 
   Size:       1046     643
 
AvrII C'CTAG_G

Cuts at:      0      21    1689 
   Size:         21    1668
 
BamHI G'GATC_C

Cuts at:      0     207    1448    1475    1626    1689 
   Size:        207    1241      27     151      63

  Fragments arranged by size: 

                1241     207     151      63      27
 
BanI G'GyrC_C

Cuts at:      0     655     850    1689 
   Size:        655     195     839

  Fragments arranged by size: 

                 839     655     195
 
BanII G_rGCy'C

Cuts at:      0      29      74     324     652     908    1031    1294    1689 
   Size:         29      45     250     328     256     123     263     395

  Fragments arranged by size: 

                 395     328     263     256     250     123      45      29
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1116    1678    1689 
   Size:       1116     562      11

  Fragments arranged by size: 

                1116     562      11
 
BbvCI CC'TCA_GC

Cuts at:      0     994    1689 
   Size:        994     695
 
BclI T'GATC_A

Cuts at:      0     341    1460    1689 
   Size:        341    1119     229

  Fragments arranged by size: 

                1119     341     229
 
BlpI GC'TnA_GC

Cuts at:      0     370    1689 
   Size:        370    1319
 
Bme1580I G_kGCm'C

Cuts at:      0     684    1400    1689 
   Size:        684     716     289

  Fragments arranged by size: 

                 716     684     289
 
BmgBI CAC'GTC

Cuts at:      0     645    1689 
   Size:        645    1044
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     678    1386    1689 
   Size:        678     708     303

  Fragments arranged by size: 

                 708     678     303
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712    1125    1418    1689 
   Size:        712     413     293     271

  Fragments arranged by size: 

                 712     413     293     271
 
Bpu10I CC'TnA_GC

Cuts at:      0     557     994    1288    1689 
   Size:        557     437     294     401

  Fragments arranged by size: 

                 557     437     401     294
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1057    1689 
   Size:       1057     632
 
BsaWI w'CCGG_w

Cuts at:      0    1414    1689 
   Size:       1414     275
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     269     299    1689 
   Size:        269      30    1390

  Fragments arranged by size: 

                1390     269      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     209    1689 
   Size:        209    1480
 
BseYI C'CCAG_C

Cuts at:      0     101     211    1622    1640    1659    1689 
   Size:        101     110    1411      18      19      30

  Fragments arranged by size: 

                1411     110     101      30      19      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     502     850    1343    1689 
   Size:        214     288     348     493     346

  Fragments arranged by size: 

                 493     348     346     288     214
 
BsiHKAI G_wGCw'C

Cuts at:      0    1031    1294    1472    1689 
   Size:       1031     263     178     217

  Fragments arranged by size: 

                1031     263     217     178
 
Bsp1286I G_dGCh'C

Cuts at:      0      29      74     324     652     684     908    1031    1294 
   Size:         29      45     250     328      32     224     123     263

Cuts at:   1294    1400    1472    1689 
   Size:        106      72     217

  Fragments arranged by size: 

                 328     263     250     224     217     123     106      72
                  45      32      29
 
BspEI T'CCGG_A

Cuts at:      0    1414    1689 
   Size:       1414     275
 
BspHI T'CATG_A

Cuts at:      0    1031    1689 
   Size:       1031     658
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     555    1313    1689 
   Size:        555     758     376

  Fragments arranged by size: 

                 758     555     376
 
BsrBI CCG'CTC

Cuts at:      0    1045    1689 
   Size:       1045     644
 
BsrDI GCAATG_nn'

Cuts at:      0    1245    1689 
   Size:       1245     444
 
BsrFI r'CCGG_y

Cuts at:      0     887    1689 
   Size:        887     802
 
BssSI C'ACGA_G

Cuts at:      0    1114    1689 
   Size:       1114     575
 
BstEII G'GTnAC_C

Cuts at:      0     597    1689 
   Size:        597    1092
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1531    1689 
   Size:       1531     158
 
BstYI r'GATC_y

Cuts at:      0     207    1448    1475    1626    1689 
   Size:        207    1241      27     151      63

  Fragments arranged by size: 

                1241     207     151      63      27
 
Bsu36I CC'TnA_GG

Cuts at:      0     128     544    1071    1689 
   Size:        128     416     527     618

  Fragments arranged by size: 

                 618     527     416     128
 
BtgI C'CryG_G

Cuts at:      0    1249    1405    1557    1689 
   Size:       1249     156     152     132

  Fragments arranged by size: 

                1249     156     152     132
 
BtsI GCAGTG_nn'

Cuts at:      0     922    1689 
   Size:        922     767
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     467     502    1314    1349    1689 
   Size:        467      35     812      35     340

  Fragments arranged by size: 

                 812     467     340      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     294     423    1689 
   Size:        294     129    1266

  Fragments arranged by size: 

                1266     294     129
 
EaeI y'GGCC_r

Cuts at:      0     885    1335    1485    1689 
   Size:        885     450     150     204

  Fragments arranged by size: 

                 885     450     204     150
 
EarI CTCTTCn'nnn_

Cuts at:      0     128     742    1069    1580    1689 
   Size:        128     614     327     511     109

  Fragments arranged by size: 

                 614     511     327     128     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1111    1689 
   Size:       1111     578
 
EcoICRI GAG'CTC

Cuts at:      0    1029    1292    1689 
   Size:       1029     263     397

  Fragments arranged by size: 

                1029     397     263
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712    1125    1218    1418    1689 
   Size:        712     413      93     200     271

  Fragments arranged by size: 

                 712     413     271     200      93
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1634    1689 
   Size:       1634      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     430     455    1689 
   Size:        430      25    1234

  Fragments arranged by size: 

                1234     430      25
 
HaeII r_GCGC'y

Cuts at:      0    1004    1689 
   Size:       1004     685
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     199     231     537     569     634     666     973    1005 
   Size:        199      32     306      32      65      32     307      32

Cuts at:   1005    1689 
   Size:        684

  Fragments arranged by size: 

                 684     307     306     199      65      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1020    1212    1689 
   Size:       1020     192     477

  Fragments arranged by size: 

                1020     477     192
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     402     762    1689 
   Size:        185     217     360     927

  Fragments arranged by size: 

                 927     360     217     185
 
MslI CAynn'nnrTG

Cuts at:      0     419    1159    1689 
   Size:        419     740     530

  Fragments arranged by size: 

                 740     530     419
 
MspA1I CmG'CkG

Cuts at:      0      81    1014    1689 
   Size:         81     933     675

  Fragments arranged by size: 

                 933     675      81
 
NaeI GCC'GGC

Cuts at:      0     889    1689 
   Size:        889     800
 
NcoI C'CATG_G

Cuts at:      0    1405    1689 
   Size:       1405     284
 
NgoMIV G'CCGG_C

Cuts at:      0     887    1689 
   Size:        887     802
 
NspI r_CATG'y

Cuts at:      0     958    1689 
   Size:        958     731
 
PasI CC'CwG_GG

Cuts at:      0     111     400    1689 
   Size:        111     289    1289

  Fragments arranged by size: 

                1289     289     111
 
PciI A'CATG_T

Cuts at:      0     954    1689 
   Size:        954     735
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     690    1406    1441    1689 
   Size:        690     716      35     248

  Fragments arranged by size: 

                 716     690     248      35
 
PfoI T'CCnGG_A

Cuts at:      0     689    1178    1689 
   Size:        689     489     511

  Fragments arranged by size: 

                 689     511     489
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1175    1207    1689 
   Size:       1175      32     482

  Fragments arranged by size: 

                1175     482      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     430     455    1689 
   Size:        430      25    1234

  Fragments arranged by size: 

                1234     430      25
 
PstI C_TGCA'G

Cuts at:      0     917    1689 
   Size:        917     772
 
SacI G_AGCT'C

Cuts at:      0    1031    1294    1689 
   Size:       1031     263     395

  Fragments arranged by size: 

                1031     395     263
 
SapI GCTCTTCn'nnn_

Cuts at:      0     128    1069    1689 
   Size:        128     941     620

  Fragments arranged by size: 

                 941     620     128
 
SfcI C'TryA_G

Cuts at:      0     578     873     913    1689 
   Size:        578     295      40     776

  Fragments arranged by size: 

                 776     578     295      40
 
StuI AGG'CCT

Cuts at:      0     484     584    1689 
   Size:        484     100    1105

  Fragments arranged by size: 

                1105     484     100
 
StyI C'CwwG_G

Cuts at:      0      21     485    1405    1513    1689 
   Size:         21     464     920     108     176

  Fragments arranged by size: 

                 920     464     176     108      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      93    1368    1398    1665    1689 
   Size:         93    1275      30     267      24

  Fragments arranged by size: 

                1275     267      93      30      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     269     301    1467    1499    1640    1672    1689 
   Size:        269      32    1166      32     141      32      17

  Fragments arranged by size: 

                1166     269     141      32      32      32      17
 
XbaI T'CTAG_A

Cuts at:      0     822    1689 
   Size:        822     867
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     AvaI
    AvrII    BamHI     BanI    BanII     BbsI    BbvCI     BclI     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI    CspCI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     MmeI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PpuMI     PstI     SacI     SapI     SfcI     StuI
     StyI    TaqII     TstI     XbaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI     BbeI
     BcgI    BciVI    BfrBI     BglI    BglII     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstAPI    BstBI  BstZ17I    BtgZI     ClaI     DraI     DrdI
     EagI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
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