Restriction map for pFN21AB6168
 (Linear) (Six-base) MAPSORT of: KIBB6168.seq  Check: 263  from: 1  to: 1809



KIBB6168.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     958    1468    1809 
   Size:        958     510     341

  Fragments arranged by size: 

                 958     510     341
 
AccI GT'mk_AC

Cuts at:      0     724    1109    1809 
   Size:        724     385     700

  Fragments arranged by size: 

                 724     700     385
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841    1492    1809 
   Size:        841     651     317

  Fragments arranged by size: 

                 841     651     317
 
AleI CACnn'nnGTG

Cuts at:      0    1121    1756    1809 
   Size:       1121     635      53

  Fragments arranged by size: 

                1121     635      53
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     265    1809 
   Size:        265    1544
 
AlwNI CAG_nnn'CTG

Cuts at:      0      16     498    1140    1809 
   Size:         16     482     642     669

  Fragments arranged by size: 

                 669     642     482      16
 
AvaI C'yCGr_G

Cuts at:      0     658    1064    1809 
   Size:        658     406     745

  Fragments arranged by size: 

                 745     658     406
 
BamHI G'GATC_C

Cuts at:      0    1203    1809 
   Size:       1203     606
 
BanI G'GyrC_C

Cuts at:      0      36    1480    1809 
   Size:         36    1444     329

  Fragments arranged by size: 

                1444     329      36
 
BanII G_rGCy'C

Cuts at:      0      90     395    1514    1777    1809 
   Size:         90     305    1119     263      32

  Fragments arranged by size: 

                1119     305     263      90      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     631    1809 
   Size:        631    1178
 
BbvCI CC'TCA_GC

Cuts at:      0    1199    1573    1809 
   Size:       1199     374     236

  Fragments arranged by size: 

                1199     374     236
 
BglII A'GATC_T

Cuts at:      0     206     558    1809 
   Size:        206     352    1251

  Fragments arranged by size: 

                1251     352     206
 
BlpI GC'TnA_GC

Cuts at:      0     667    1771    1809 
   Size:        667    1104      38

  Fragments arranged by size: 

                1104     667      38
 
Bme1580I G_kGCm'C

Cuts at:      0      39     683    1633    1809 
   Size:         39     644     950     176

  Fragments arranged by size: 

                 950     644     176      39
 
BmgBI CAC'GTC

Cuts at:      0     336    1809 
   Size:        336    1473
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     583    1720    1809 
   Size:        436     147    1137      89

  Fragments arranged by size: 

                1137     436     147      89
 
Bpu10I CC'TnA_GC

Cuts at:      0    1055    1199    1573    1809 
   Size:       1055     144     374     236

  Fragments arranged by size: 

                1055     374     236     144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     514    1783    1809 
   Size:        253     261    1269      26

  Fragments arranged by size: 

                1269     261     253      26
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     413     518     766    1809 
   Size:        413     105     248    1043

  Fragments arranged by size: 

                1043     413     248     105
 
BsaWI w'CCGG_w

Cuts at:      0     782    1007    1809 
   Size:        782     225     802

  Fragments arranged by size: 

                 802     782     225
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     277     307    1809 
   Size:        277      30    1502

  Fragments arranged by size: 

                1502     277      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     444    1207    1717    1809 
   Size:        444     763     510      92

  Fragments arranged by size: 

                 763     510     444      92
 
BseYI C'CCAG_C

Cuts at:      0     534     985    1000    1809 
   Size:        534     451      15     809

  Fragments arranged by size: 

                 809     534     451      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     484    1126    1552    1609    1809 
   Size:        406      78     642     426      57     200

  Fragments arranged by size: 

                 642     426     406     200      78      57
 
BsiEI CG_ry'CG

Cuts at:      0      44    1809 
   Size:         44    1765
 
BsiHKAI G_wGCw'C

Cuts at:      0      90     395    1304    1466    1514    1777    1809 
   Size:         90     305     909     162      48     263      32

  Fragments arranged by size: 

                 909     305     263     162      90      48      32
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     943    1801    1809 
   Size:        943     858       8

  Fragments arranged by size: 

                 943     858       8
 
Bsp1286I G_dGCh'C

Cuts at:      0      39      90     395     683    1304    1466    1514    1633 
   Size:         39      51     305     288     621     162      48     119

Cuts at:   1633    1777    1809 
   Size:        144      32

  Fragments arranged by size: 

                 621     305     288     162     144     119      51      48
                  39      32
 
BspEI T'CCGG_A

Cuts at:      0     782    1809 
   Size:        782    1027
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     506     958    1468    1809 
   Size:        506     452     510     341

  Fragments arranged by size: 

                 510     506     452     341
 
BsrFI r'CCGG_y

Cuts at:      0      39     105     146    1733    1809 
   Size:         39      66      41    1587      76

  Fragments arranged by size: 

                1587      76      66      41      39
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      16     480    1140    1809 
   Size:         16     464     660     669

  Fragments arranged by size: 

                 669     660     464      16
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     993    1809 
   Size:        993     816
 
BstYI r'GATC_y

Cuts at:      0     116     206     558     864    1203    1809 
   Size:        116      90     352     306     339     606

  Fragments arranged by size: 

                 606     352     339     306     116      90
 
BstZ17I GTA'TAC

Cuts at:      0     725    1809 
   Size:        725    1084
 
Bsu36I CC'TnA_GG

Cuts at:      0     452    1809 
   Size:        452    1357
 
BtgI C'CryG_G

Cuts at:      0     296     348     827    1809 
   Size:        296      52     479     982

  Fragments arranged by size: 

                 982     479     296      52
 
BtsI GCAGTG_nn'

Cuts at:      0      17     869    1809 
   Size:         17     852     940

  Fragments arranged by size: 

                 940     852      17
 
EaeI y'GGCC_r

Cuts at:      0      41     830    1727    1784    1809 
   Size:         41     789     897      57      25

  Fragments arranged by size: 

                 897     789      57      41      25
 
EagI C'GGCC_G

Cuts at:      0      41    1809 
   Size:         41    1768
 
EarI CTCTTCn'nnn_

Cuts at:      0    1309    1809 
   Size:       1309     500
 
EcoICRI GAG'CTC

Cuts at:      0      88     393    1512    1775    1809 
   Size:         88     305    1119     263      34

  Fragments arranged by size: 

                1119     305     263      88      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     583     841    1492    1720    1809 
   Size:        436     147     258     651     228      89

  Fragments arranged by size: 

                 651     436     258     228     147      89
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     126    1809 
   Size:         63      63    1683

  Fragments arranged by size: 

                1683      63      63
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     204    1641    1689    1809 
   Size:        204    1437      48     120

  Fragments arranged by size: 

                1437     204     120      48
 
HaeII r_GCGC'y

Cuts at:      0     156    1559    1809 
   Size:        156    1403     250

  Fragments arranged by size: 

                1403     250     156
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309     688     720    1386    1418    1809 
   Size:        277      32     379      32     666      32     391

  Fragments arranged by size: 

                 666     391     379     277      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1110    1809 
   Size:       1110     699
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368    1809 
   Size:        368    1441
 
MscI TGG'CCA

Cuts at:      0    1729    1809 
   Size:       1729      80
 
MslI CAynn'nnrTG

Cuts at:      0    1121    1756    1809 
   Size:       1121     635      53

  Fragments arranged by size: 

                1121     635      53
 
MspA1I CmG'CkG

Cuts at:      0    1140    1809 
   Size:       1140     669
 
NaeI GCC'GGC

Cuts at:      0     148    1735    1809 
   Size:        148    1587      74

  Fragments arranged by size: 

                1587     148      74
 
NcoI C'CATG_G

Cuts at:      0     296    1809 
   Size:        296    1513
 
NgoMIV G'CCGG_C

Cuts at:      0     146    1733    1809 
   Size:        146    1587      76

  Fragments arranged by size: 

                1587     146      76
 
NspI r_CATG'y

Cuts at:      0    1131    1809 
   Size:       1131     678
 
PasI CC'CwG_GG

Cuts at:      0      49    1809 
   Size:         49    1760
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1226    1809 
   Size:       1226     583
 
PfoI T'CCnGG_A

Cuts at:      0      81     119     413    1604    1697    1809 
   Size:         81      38     294    1191      93     112

  Fragments arranged by size: 

                1191     294     112      93      81      38
 
PpuMI rG'GwC_Cy

Cuts at:      0      63     126    1809 
   Size:         63      63    1683

  Fragments arranged by size: 

                1683      63      63
 
PstI C_TGCA'G

Cuts at:      0      12      21    1136    1355    1809 
   Size:         12       9    1115     219     454

  Fragments arranged by size: 

                1115     454     219      12       9
 
PvuII CAG'CTG

Cuts at:      0    1140    1809 
   Size:       1140     669
 
SacI G_AGCT'C

Cuts at:      0      90     395    1514    1777    1809 
   Size:         90     305    1119     263      32

  Fragments arranged by size: 

                1119     305     263      90      32
 
SalI G'TCGA_C

Cuts at:      0    1108    1809 
   Size:       1108     701
 
SanDI GG'GwC_CC

Cuts at:      0      63    1809 
   Size:         63    1746
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1309    1809 
   Size:       1309     500
 
SexAI A'CCwGG_T

Cuts at:      0    1537    1809 
   Size:       1537     272
 
SfcI C'TryA_G

Cuts at:      0       8      17    1132    1351    1809 
   Size:          8       9    1115     219     458

  Fragments arranged by size: 

                1115     458     219       9       8
 
SgrAI Cr'CCGG_yG

Cuts at:      0     105    1809 
   Size:        105    1704
 
SmaI CCC'GGG

Cuts at:      0    1066    1809 
   Size:       1066     743
 
SmlI C'TyrA_G

Cuts at:      0     268     529    1798    1809 
   Size:        268     261    1269      11

  Fragments arranged by size: 

                1269     268     261      11
 
SspI AAT'ATT

Cuts at:      0    1441    1809 
   Size:       1441     368
 
StuI AGG'CCT

Cuts at:      0    1804    1809 
   Size:       1804       5
 
StyI C'CwwG_G

Cuts at:      0     224     296     967    1266    1809 
   Size:        224      72     671     299     543

  Fragments arranged by size: 

                 671     543     299     224      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     701    1230    1809 
   Size:        701     529     579

  Fragments arranged by size: 

                 701     579     529
 
TatI w'GTAC_w

Cuts at:      0    1355    1809 
   Size:       1355     454
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1310    1342    1809 
   Size:       1310      32     467

  Fragments arranged by size: 

                1310     467      32
 
XmaI C'CCGG_G

Cuts at:      0    1064    1809 
   Size:       1064     745
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AlfI    AlwNI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BglII     BlpI Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI
   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI    PpuMI     PstI    PvuII     SacI
     SalI    SanDI     SapI    SexAI     SfcI    SgrAI     SmaI     SmlI
     SspI     StuI     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BmrI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsiWI     BsmI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SbfI     ScaI     SfiI
     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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