################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII BalI BbrPI BbsI BcgI BciVI BclI BfrI BglII BpuAI BsaAI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfuI SgrAI SnaBI SpeI SspI SwaI TfiI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 881 GGTACC G^GTACC AccI 2098 GTCTAC GT^[AC][GT]AC AcsI 718 AAATTC [GA]^AATT[CT] AocI 1575 CCTGAGG CC^T[ACGT]AGG ApoI 718 AAATTC [GA]^AATT[CT] Asp718I 881 GGTACC G^GTACC AspI 418 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 696 CCTAGG C^CTAGG BamHI 1899 GGATCC G^GATCC BbeI 1358 GGCGCC GGCGC^C BlnI 696 CCTAGG C^CTAGG Bsp1407I 2024 TGTACA T^GTACA BsrFI 1361 GCCGGC [GA]^CCGG[CT] BsrGI 2024 TGTACA T^GTACA BssSI 274 CTCGTG CACGAG(-5/-1) BstAPI 1907 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1899 GGATCC [GA]^GATC[CT] Bsu36I 1575 CCTGAGG CC^T[ACGT]AGG Cfr10I 1361 GCCGGC [GA]^CCGG[CT] DpnII 1900 GATC ^GATC DsaI 1133 CCGCGG C^C[GA][CT]GG EarI 2017 CTCTTC CTCTTC(1/4) Eco81I 1575 CCTGAGG CC^T[ACGT]AGG HgaI 383 GCGTC GACGC(5/10) HindIII 729 AAGCTT A^AGCTT HinfI 16 GAGTC G^A[ACGT]TC KasI 1358 GGCGCC G^GCGCC KpnI 881 GGTACC GGTAC^C Ksp632I 2017 CTCTTC CTCTTC(1/4) KspI 1133 CCGCGG CCGC^GG MboI 1900 GATC ^GATC MflI 1899 GGATCC [GA]^GATC[CT] NaeI 1361 GCCGGC GCC^GGC NarI 1358 GGCGCC GG^CGCC NdeII 1900 GATC ^GATC NgoMIV 1361 GCCGGC G^CCGGC NotI 229 GCGGCCGC GC^GGCCGC PflFI 418 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 933 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 16 GAGTC GAGTC(4/5) SacII 1133 CCGCGG CCGC^GG Sau3AI 1900 GATC ^GATC SbfI 1545 CCTGCAGG CCTGCA^GG SfiI 143 GGCCGCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1358 GGCGCC GGC^GCC SphI 314 GCATGC GCATG^C Sse8387I 1545 CCTGCAGG CCTGCA^GG SspBI 2024 TGTACA T^GTACA TaqI 1951 TCGA T^CGA Tsp509I 719 AATT ^AATT Tth111I 418 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1951 TCGA T^CGA Van91I 933 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1899 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 382 GGCGTC G[GA]^CG[CT]C 1358 GGCGCC AflIII 1287 ACATGT A^C[GA][CT]GT 1682 ACATGT AlwI 1900 GATCC GGATC(4/5) 1899 GGATC BsaHI 382 GGCGTC G[GA]^CG[CT]C 1358 GGCGCC BsaI 754 GAGACC GGTCTC(1/5) 2122 GAGACC BsaWI 652 ACCGGA [AT]^CCGG[AT] 1077 ACCGGA BsiEI 151 CGGCCG CG[GA][CT]^CG 230 CGGCCG BsmBI 348 CGTCTC CGTCTC(1/5) 384 CGTCTC BspLU11I 1287 ACATGT A^CATGT 1682 ACATGT BspMI 777 ACCTGC ACCTGC(4/8) 1086 GCAGGT BsrBI 835 GAGCGG CCGCTC(-3/-3) 1081 GAGCGG BsrDI 1304 CATTGC GCAATG(2/0) 1309 CATTGC BstXI 1922 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2199 CCAGGGAGATGG EagI 151 CGGCCG C^GGCCG 230 CGGCCG EclXI 151 CGGCCG C^GGCCG 230 CGGCCG Eco52I 151 CGGCCG C^GGCCG 230 CGGCCG EcoT14I 389 CCTTGG C^C[AT][AT]GG 696 CCTAGG Hin1I 382 GGCGTC G[GA]^CG[CT]C 1358 GGCGCC MaeII 110 ACGT A^CGT 268 ACGT MboII 671 TCTTC GAAGA(8/7) 2018 TCTTC SmlI 632 CTCAAG C^T[CT][GA]AG 1321 CTCAAG StuI 1550 AGGCCT AGG^CCT 1946 AGGCCT StyI 389 CCTTGG C^C[AT][AT]GG 696 CCTAGG XcmI 527 CCATCTCCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1164 CCAGGTGCGGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DraII EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV NspI PpuMI PstI PvuII RsaI SacI Sau96I ScrFI SfaNI SfcI SmaI TseI Tsp45I TspRI XmaCI XmaI