(Circular) (Six-base) MAPSORT of: pFN21AE1381.seq Check: 9019 from: 1 to: 7054 pFN21AE1381 7054 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2791 3082 2791 Size: 291 6763 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4864 278 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4104 4397 1937 Size: 2167 293 4594 Fragments arranged by size: 4594 2167 293 Acc65I G'GTAC_C Cuts at: 2886 4375 2886 Size: 1489 5565 AclI AA'CG_TT Cuts at: 1563 5181 5554 1563 Size: 3618 373 3063 Fragments arranged by size: 3618 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 2642 4293 5117 6501 6769 1632 Size: 720 290 1651 824 1384 268 1917 Fragments arranged by size: 1917 1651 1384 824 720 290 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6857 AflIII A'CryG_T Cuts at: 1203 3292 3687 5953 6930 1203 Size: 2089 395 2266 977 1327 Fragments arranged by size: 2266 2089 1327 977 395 AgeI A'CCGG_T Cuts at: 5885 5885 Size: 7054 AhdI GACnn_n'nnGTC Cuts at: 5783 5783 Size: 7054 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7054 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2342 1184 Size: 1158 5896 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2399 2431 2399 Size: 32 7022 AlwNI CAG_nnn'CTG Cuts at: 2314 3199 3220 3499 3840 3918 4797 6369 2314 Size: 885 21 279 341 78 879 1572 2999 Fragments arranged by size: 2999 1572 885 879 341 279 78 21 ApaI G_GGCC'C Cuts at: 2594 2709 3186 3232 3260 3427 3434 4069 2594 Size: 115 477 46 28 167 7 635 5579 Fragments arranged by size: 5579 635 477 167 115 46 28 7 ApaLI G'TGCA_C Cuts at: 5111 6267 5111 Size: 1156 5898 ApoI r'AATT_y Cuts at: 1493 2723 4363 4589 4741 6850 1493 Size: 1230 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 1230 226 152 AseI AT'TA_AT Cuts at: 160 1032 5608 160 Size: 872 4576 1606 Fragments arranged by size: 4576 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 AvaI C'yCGr_G Cuts at: 1870 1943 2266 2457 2692 2994 3185 3231 3420 Size: 73 323 191 235 302 191 46 189 Cuts at: 3420 4379 1870 Size: 959 4545 Fragments arranged by size: 4545 959 323 302 235 191 191 189 73 46 AvrII C'CTAG_G Cuts at: 2701 2701 Size: 7054 BamHI G'GATC_C Cuts at: 3904 4384 4751 3904 Size: 480 367 6207 Fragments arranged by size: 6207 480 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2654 2886 2983 3079 Size: 325 217 627 286 581 232 97 96 Cuts at: 3079 3363 3608 4200 4375 5830 618 Size: 284 245 592 175 1455 1842 Fragments arranged by size: 1842 1455 627 592 581 325 286 284 245 232 217 175 97 96 BanII G_rGCy'C Cuts at: 1391 2036 2047 2265 2381 2405 2412 2564 2594 Size: 645 11 218 116 24 7 152 30 Cuts at: 2594 2709 2879 3005 3186 3192 3232 3260 3427 Size: 115 170 126 181 6 40 28 167 Cuts at: 3427 3434 3708 3809 4069 4373 1391 Size: 7 274 101 260 304 4072 Fragments arranged by size: 4072 645 304 274 260 218 181 170 167 152 126 116 115 101 40 30 28 24 11 7 7 6 BbeI G_GCGC'C Cuts at: 3367 3367 Size: 7054 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6083 BbvCI CC'TCA_GC Cuts at: 2252 3402 3833 2252 Size: 1150 431 5473 Fragments arranged by size: 5473 1150 431 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5245 5279 1903 Size: 34 3308 34 3678 Fragments arranged by size: 3678 3308 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4946 6156 4946 Size: 1210 5844 BclI T'GATC_A Cuts at: 1548 1548 Size: 7054 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2155 2209 2385 Size: 107 122 71 631 605 483 54 176 Cuts at: 2385 3283 3337 3761 5665 136 Size: 898 54 424 1904 1525 Fragments arranged by size: 1904 1525 898 631 605 483 424 176 122 107 71 54 54 BglII A'GATC_T Cuts at: 7049 7049 Size: 7054 BlpI GC'TnA_GC Cuts at: 2583 3221 3632 4308 4471 2583 Size: 638 411 676 163 5166 Fragments arranged by size: 5166 676 638 411 163 Bme1580I G_kGCm'C Cuts at: 1790 2076 2121 2594 2657 2709 3186 3232 3260 Size: 286 45 473 63 52 477 46 28 Cuts at: 3260 3427 3434 3945 4069 5115 6271 1790 Size: 167 7 511 124 1046 1156 2573 Fragments arranged by size: 2573 1156 1046 511 477 473 286 167 124 63 52 46 45 28 7 BmgBI CAC'GTC Cuts at: 1318 2116 1318 Size: 798 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2079 2600 3464 Size: 416 53 83 399 24 662 521 864 Cuts at: 3464 4785 5743 442 Size: 1321 958 1753 Fragments arranged by size: 1753 1321 958 864 662 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7054 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 7054 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 2061 2341 2393 2546 2578 2619 2749 Size: 798 24 280 52 153 32 41 130 Cuts at: 2749 3320 3695 3722 3741 5714 1239 Size: 571 375 27 19 1973 2579 Fragments arranged by size: 2579 1973 798 571 375 280 153 130 52 41 32 27 24 19 Bpu10I CC'TnA_GC Cuts at: 2252 3402 3455 3663 3833 2252 Size: 1150 53 208 170 5473 Fragments arranged by size: 5473 1150 208 170 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2622 3311 5179 6044 6342 2622 Size: 689 1868 865 298 3334 Fragments arranged by size: 3334 1868 865 689 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2753 4121 5717 882 Size: 617 1254 1368 1596 2219 Fragments arranged by size: 2219 1596 1368 1254 617 BsaAI yAC'GTr Cuts at: 493 1204 4845 493 Size: 711 3641 2702 Fragments arranged by size: 3641 2702 711 BsaBI GATnn'nnATC Cuts at: 7048 7048 Size: 7054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2388 3364 4861 5243 Size: 53 83 186 936 855 976 1497 382 Cuts at: 5243 5878 275 Size: 635 1451 Fragments arranged by size: 1497 1451 976 936 855 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2657 3082 5487 5885 6159 6306 6717 2657 Size: 425 2405 398 274 147 411 2994 Fragments arranged by size: 2994 2405 425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2399 2429 2592 2622 2939 Size: 30 165 30 996 30 163 30 317 Cuts at: 2939 2969 3668 3698 4184 4214 1178 Size: 30 699 30 486 30 4018 Fragments arranged by size: 4018 996 699 486 317 165 163 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2462 2554 2987 2990 3875 4163 4166 4205 Size: 1276 92 433 3 885 288 3 39 Cuts at: 4205 4364 1186 Size: 159 3876 Fragments arranged by size: 3876 1276 885 433 288 159 92 39 3 3 BseYI C'CCAG_C Cuts at: 2048 2616 2828 2960 3004 3179 3253 3527 3707 Size: 568 212 132 44 175 74 274 180 Cuts at: 3707 3908 4002 4220 6257 6753 2048 Size: 201 94 218 2037 496 2349 Fragments arranged by size: 2349 2037 568 496 274 218 212 201 180 175 132 94 74 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 3173 3833 3904 1163 Size: 573 492 945 660 71 4313 Fragments arranged by size: 4313 945 660 573 492 71 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2159 2238 4504 5265 6293 1540 Size: 275 186 158 79 2266 761 1028 2301 Fragments arranged by size: 2301 2266 1028 761 275 186 158 79 BsiHKAI G_wGCw'C Cuts at: 2412 2564 2879 3327 5115 5200 6271 2412 Size: 152 315 448 1788 85 1071 3195 Fragments arranged by size: 3195 1788 1071 448 315 152 85 BsmI GAATG_Cn' Cuts at: 4565 4658 4565 Size: 93 6961 BsmBI CGTCTCn'nnnn_ Cuts at: 2359 2395 2359 Size: 36 7018 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2047 2076 2121 2265 2381 2405 Size: 399 246 11 29 45 144 116 24 Cuts at: 2405 2412 2564 2594 2657 2709 2879 3005 3186 Size: 7 152 30 63 52 170 126 181 Cuts at: 3186 3192 3232 3260 3327 3427 3434 3708 3809 Size: 6 40 28 67 100 7 274 101 Cuts at: 3809 3945 4069 4373 5115 5200 6271 1391 Size: 136 124 304 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 304 274 246 181 170 152 144 136 126 124 116 101 100 85 67 63 52 45 40 30 29 28 24 11 7 7 6 BspEI T'CCGG_A Cuts at: 6717 6717 Size: 7054 BspHI T'CATG_A Cuts at: 4943 6673 4943 Size: 1730 5324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2791 3082 4409 844 Size: 1947 291 1327 3489 Fragments arranged by size: 3489 1947 1327 291 BsrBI CCG'CTC Cuts at: 1394 1823 2842 3088 4941 1394 Size: 429 1019 246 1853 3507 Fragments arranged by size: 3507 1853 1019 429 246 BsrDI GCAATG_nn' Cuts at: 65 1248 3308 3313 5549 5723 65 Size: 1183 2060 5 2236 174 1396 Fragments arranged by size: 2236 2060 1396 1183 174 5 BsrFI r'CCGG_y Cuts at: 1700 1810 3366 5698 5885 1700 Size: 110 1556 2332 187 2869 Fragments arranged by size: 2869 2332 1556 187 110 BsrGI T'GTAC_A Cuts at: 96 4029 96 Size: 3933 3121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7054 BssSI C'ACGA_G Cuts at: 2279 5114 6126 2279 Size: 2835 1012 3207 Fragments arranged by size: 3207 2835 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3918 794 Size: 3124 3930 BstBI TT'CG_AA Cuts at: 5858 5858 Size: 7054 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7054 BstXI CCAn_nnnn'nTGG Cuts at: 3934 4211 3934 Size: 277 6777 BstYI r'GATC_y Cuts at: 1972 3904 4384 4751 5136 5153 6605 6691 6703 Size: 1932 480 367 385 17 1452 86 12 Cuts at: 6703 7049 1972 Size: 346 1977 Fragments arranged by size: 1977 1932 1452 480 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3581 3581 Size: 7054 BtgI C'CryG_G Cuts at: 513 1065 2004 3138 513 Size: 552 939 1134 4429 Fragments arranged by size: 4429 1134 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6315 Fragments arranged by size: 6315 683 56 BtsI GCAGTG_nn' Cuts at: 2240 2911 3868 3919 4165 4570 5364 5384 2240 Size: 671 957 51 246 405 794 20 3910 Fragments arranged by size: 3910 957 794 671 405 246 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7054 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7019 DraI TTT'AAA Cuts at: 4358 4358 Size: 7054 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6061 809 Size: 507 4745 1802 Fragments arranged by size: 4745 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2156 2235 3936 Size: 54 1425 50 275 18 326 79 1701 Cuts at: 3936 4501 5390 7019 8 Size: 565 889 1629 43 Fragments arranged by size: 1701 1629 1425 889 565 326 275 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2156 2235 4501 1537 Size: 275 344 79 2266 4090 Fragments arranged by size: 4090 2266 344 275 79 EarI CTCTTCn'nnn_ Cuts at: 1353 4028 4984 1353 Size: 2675 956 3423 Fragments arranged by size: 3423 2675 956 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2910 3401 5641 6015 6161 387 Size: 1064 138 1321 491 2240 374 146 1280 Fragments arranged by size: 2240 1321 1280 1064 491 374 146 138 EcoICRI GAG'CTC Cuts at: 2410 2562 2877 2410 Size: 152 315 6587 Fragments arranged by size: 6587 315 152 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2061 2341 2352 2393 2546 2578 Size: 393 405 24 280 11 41 153 32 Cuts at: 2578 2619 2642 2749 3320 3695 3722 3741 4293 Size: 41 23 107 571 375 27 19 552 Cuts at: 4293 5117 5714 6501 6769 1239 Size: 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 597 571 552 405 393 375 280 268 153 107 41 41 32 27 24 23 19 11 EcoNI CCTnn'n_nnAGG Cuts at: 2939 3401 3782 4031 2939 Size: 462 381 249 5962 Fragments arranged by size: 5962 462 381 249 EcoO109I rG'GnC_Cy Cuts at: 2101 2311 2590 2698 2705 2991 3157 3257 3406 Size: 210 279 108 7 286 166 100 149 Cuts at: 3406 3430 3431 3447 3585 4065 4066 4301 2101 Size: 24 1 16 138 480 1 235 4854 Fragments arranged by size: 4854 480 286 279 235 210 166 149 138 108 100 24 16 7 1 1 EcoRI G'AATT_C Cuts at: 4363 4741 4363 Size: 378 6676 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2752 2752 Size: 7054 FspI TGC'GCA Cuts at: 5560 5560 Size: 7054 HaeII r_GCGC'y Cuts at: 1678 2188 3060 3221 3367 3534 3829 3853 6201 Size: 510 872 161 146 167 295 24 2348 Cuts at: 6201 1678 Size: 2531 Fragments arranged by size: 2531 2348 872 510 295 167 161 146 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2939 2971 3674 3706 5695 Size: 32 135 32 1367 32 703 32 1989 Cuts at: 5695 5727 5769 5801 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1989 1367 703 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4398 4644 669 Size: 988 281 2460 246 3079 Fragments arranged by size: 3079 2460 988 281 246 HindIII A'AGCT_T Cuts at: 748 2734 748 Size: 1986 5068 HpaI GTT'AAC Cuts at: 4644 4644 Size: 7054 KasI G'GCGC_C Cuts at: 3363 3363 Size: 7054 KpnI G_GTAC'C Cuts at: 2890 4379 2890 Size: 1489 5565 MfeI C'AATT_G Cuts at: 4653 4653 Size: 7054 MluI A'CGCG_T Cuts at: 6930 6930 Size: 7054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3459 3672 6168 6352 1305 Size: 2154 213 2496 184 2007 Fragments arranged by size: 2496 2154 2007 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5232 Fragments arranged by size: 5232 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5012 5371 5530 518 Size: 648 3846 359 159 2042 Fragments arranged by size: 3846 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2130 2155 2218 2234 2479 2587 2620 2860 Size: 524 25 63 16 245 108 33 240 Cuts at: 2860 3140 3148 3508 4470 5147 6295 6540 1606 Size: 280 8 360 962 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 677 524 360 280 245 245 240 108 63 33 25 16 8 NaeI GCC'GGC Cuts at: 3368 3368 Size: 7054 NarI GG'CG_CC Cuts at: 3364 3364 Size: 7054 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5563 Fragments arranged by size: 5563 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7054 NgoMIV G'CCGG_C Cuts at: 3366 3366 Size: 7054 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7054 NotI GC'GGCC_GC Cuts at: 2235 4501 2235 Size: 2266 4788 NspI r_CATG'y Cuts at: 1132 1229 2251 2323 3296 3691 4412 5957 1132 Size: 97 1022 72 973 395 721 1545 2229 Fragments arranged by size: 2229 1545 1022 973 721 395 97 72 PasI CC'CwG_GG Cuts at: 1348 3388 3427 4061 4132 4239 1348 Size: 2040 39 634 71 107 4163 Fragments arranged by size: 4163 2040 634 107 71 39 PciI A'CATG_T Cuts at: 3292 3687 5953 3292 Size: 395 2266 4393 Fragments arranged by size: 4393 2266 395 PflMI CCAn_nnn'nTGG Cuts at: 1108 2944 1108 Size: 1836 5218 PfoI T'CCnGG_A Cuts at: 2038 2370 3297 2038 Size: 332 927 5795 Fragments arranged by size: 5795 927 332 PmeI GTTT'AAAC Cuts at: 4358 4358 Size: 7054 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2399 2431 5123 5155 6636 6668 2399 Size: 32 2692 32 1481 32 2785 Fragments arranged by size: 2785 2692 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2698 2991 3157 3447 3585 2101 Size: 597 293 166 290 138 5570 Fragments arranged by size: 5570 597 293 290 166 138 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7054 PsiI TTA'TAA Cuts at: 4624 4624 Size: 7054 PspOMI G'GGCC_C Cuts at: 2590 2705 3182 3228 3256 3423 3430 4065 2590 Size: 115 477 46 28 167 7 635 5579 Fragments arranged by size: 5579 635 477 167 115 46 28 7 PstI C_TGCA'G Cuts at: 830 2217 2484 3204 3507 3555 4406 830 Size: 1387 267 720 303 48 851 3478 Fragments arranged by size: 3478 1387 851 720 303 267 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7054 PvuII CAG'CTG Cuts at: 2218 2620 2860 3148 3508 2218 Size: 402 240 288 360 5764 Fragments arranged by size: 5764 402 360 288 240 SacI G_AGCT'C Cuts at: 2412 2564 2879 2412 Size: 152 315 6587 Fragments arranged by size: 6587 315 152 SacII CC_GC'GG Cuts at: 3141 3141 Size: 7054 SalI G'TCGA_C Cuts at: 1936 4396 1936 Size: 2460 4594 SanDI GG'GwC_CC Cuts at: 2991 3447 2991 Size: 456 6598 SbfI CC_TGCA'GG Cuts at: 3555 4406 3555 Size: 851 6203 ScaI AGT'ACT Cuts at: 5302 7014 5302 Size: 1712 5342 SfcI C'TryA_G Cuts at: 826 1045 2213 2480 3200 3503 3551 4402 5537 Size: 219 1168 267 720 303 48 851 1135 Cuts at: 5537 6218 6409 826 Size: 681 191 1471 Fragments arranged by size: 1471 1168 1135 851 720 681 303 267 219 191 48 SfiI GGCCn_nnn'nGGCC Cuts at: 2155 2155 Size: 7054 SfoI GGC'GCC Cuts at: 3365 3365 Size: 7054 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7054 SmaI CCC'GGG Cuts at: 1872 2268 2459 2694 2996 3233 3422 4381 1872 Size: 396 191 235 302 237 189 959 4545 Fragments arranged by size: 4545 959 396 302 237 235 191 189 SmlI C'TyrA_G Cuts at: 820 1017 1943 2637 3326 5158 6059 6321 820 Size: 197 926 694 689 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 694 689 262 197 SnaBI TAC'GTA Cuts at: 493 4845 493 Size: 4352 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7054 SphI G_CATG'C Cuts at: 1132 2323 4412 1132 Size: 1191 2089 3774 Fragments arranged by size: 3774 2089 1191 SrfI GCCC'GGGC Cuts at: 3233 3422 3233 Size: 189 6865 SspI AAT'ATT Cuts at: 5 52 4978 5 Size: 47 4926 2081 Fragments arranged by size: 4926 2081 47 StuI AGG'CCT Cuts at: 1517 3557 3953 1517 Size: 2040 396 4618 Fragments arranged by size: 4618 2040 396 StyI C'CwwG_G Cuts at: 513 1065 2004 2394 2701 4493 513 Size: 552 939 390 307 1792 3074 Fragments arranged by size: 3074 1792 939 552 390 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2273 3036 3141 3541 3881 5081 5098 Size: 1255 693 763 105 400 340 1200 17 Cuts at: 5098 5251 5436 5881 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1200 763 693 445 400 340 185 153 105 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3684 4029 5300 Size: 274 80 33 51 1443 1707 345 1271 Cuts at: 5300 7012 96 Size: 1712 138 Fragments arranged by size: 1712 1707 1443 1271 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2592 2624 4184 4216 7014 7046 2592 Size: 32 1560 32 2798 32 2600 Fragments arranged by size: 2798 2600 1560 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2426 1316 Size: 1110 5944 XbaI T'CTAG_A Cuts at: 4390 4390 Size: 7054 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2539 3176 2539 Size: 637 6417 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7054 XmaI C'CCGG_G Cuts at: 1870 2266 2457 2692 2994 3231 3420 4379 1870 Size: 396 191 235 302 237 189 959 4545 Fragments arranged by size: 4545 959 396 302 237 235 191 189 XmnI GAAnn'nnTTC Cuts at: 5183 5183 Size: 7054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4862 276 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SwaI