(Linear) (Six-base) MAPSORT of: KIEE1381.seq Check: 314 from: 1 to: 2349 KIEE1381.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 786 1077 2349 Size: 786 291 1272 Fragments arranged by size: 1272 786 291 AccI GT'mk_AC Cuts at: 0 2099 2349 Size: 2099 250 Acc65I G'GTAC_C Cuts at: 0 881 2349 Size: 881 1468 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 347 637 2288 2349 Size: 347 290 1651 61 Fragments arranged by size: 1651 347 290 61 AflIII A'CryG_T Cuts at: 0 1287 1682 2349 Size: 1287 395 667 Fragments arranged by size: 1287 667 395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 337 2349 Size: 337 2012 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 394 426 2349 Size: 394 32 1923 Fragments arranged by size: 1923 394 32 AlwNI CAG_nnn'CTG Cuts at: 0 309 1194 1215 1494 1835 1913 2349 Size: 309 885 21 279 341 78 436 Fragments arranged by size: 885 436 341 309 279 78 21 ApaI G_GGCC'C Cuts at: 0 589 704 1181 1227 1255 1422 1429 2064 Size: 589 115 477 46 28 167 7 635 Cuts at: 2064 2349 Size: 285 Fragments arranged by size: 635 589 477 285 167 115 46 28 7 ApoI r'AATT_y Cuts at: 0 718 2349 Size: 718 1631 AvaI C'yCGr_G Cuts at: 0 261 452 687 989 1180 1226 1415 2349 Size: 261 191 235 302 191 46 189 934 Fragments arranged by size: 934 302 261 235 191 191 189 46 AvrII C'CTAG_G Cuts at: 0 696 2349 Size: 696 1653 BamHI G'GATC_C Cuts at: 0 1899 2349 Size: 1899 450 BanI G'GyrC_C Cuts at: 0 68 649 881 978 1074 1358 1603 2195 Size: 68 581 232 97 96 284 245 592 Cuts at: 2195 2349 Size: 154 Fragments arranged by size: 592 581 284 245 232 154 97 96 68 BanII G_rGCy'C Cuts at: 0 31 42 260 376 400 407 559 589 Size: 31 11 218 116 24 7 152 30 Cuts at: 589 704 874 1000 1181 1187 1227 1255 1422 Size: 115 170 126 181 6 40 28 167 Cuts at: 1422 1429 1703 1804 2064 2349 Size: 7 274 101 260 285 Fragments arranged by size: 285 274 260 218 181 170 167 152 126 116 115 101 40 31 30 28 24 11 7 7 6 BbeI G_GCGC'C Cuts at: 0 1362 2349 Size: 1362 987 BbvCI CC'TCA_GC Cuts at: 0 247 1397 1828 2349 Size: 247 1150 431 521 Fragments arranged by size: 1150 521 431 247 BglI GCCn_nnn'nGGC Cuts at: 0 150 204 380 1278 1332 1756 2349 Size: 150 54 176 898 54 424 593 Fragments arranged by size: 898 593 424 176 150 54 54 BlpI GC'TnA_GC Cuts at: 0 578 1216 1627 2303 2349 Size: 578 638 411 676 46 Fragments arranged by size: 676 638 578 411 46 Bme1580I G_kGCm'C Cuts at: 0 71 116 589 652 704 1181 1227 1255 Size: 71 45 473 63 52 477 46 28 Cuts at: 1255 1422 1429 1940 2064 2349 Size: 167 7 511 124 285 Fragments arranged by size: 511 477 473 285 167 124 71 63 52 46 45 28 7 BmgBI CAC'GTC Cuts at: 0 111 2349 Size: 111 2238 BmrI ACTGGGnnnn_n' Cuts at: 0 74 595 1459 2349 Size: 74 521 864 890 Fragments arranged by size: 890 864 521 74 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 546 2349 Size: 546 1803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 56 336 388 541 573 614 744 Size: 32 24 280 52 153 32 41 130 Cuts at: 744 1315 1690 1717 1736 2349 Size: 571 375 27 19 613 Fragments arranged by size: 613 571 375 280 153 130 52 41 32 32 27 24 19 Bpu10I CC'TnA_GC Cuts at: 0 247 1397 1450 1658 1828 2349 Size: 247 1150 53 208 170 521 Fragments arranged by size: 1150 521 247 208 170 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 617 1306 2349 Size: 617 689 1043 Fragments arranged by size: 1043 689 617 BsaI GGTCTCn'nnnn_ Cuts at: 0 748 2116 2349 Size: 748 1368 233 Fragments arranged by size: 1368 748 233 BsaHI Gr'CG_yC Cuts at: 0 383 1359 2349 Size: 383 976 990 Fragments arranged by size: 990 976 383 BsaWI w'CCGG_w Cuts at: 0 652 1077 2349 Size: 652 425 1272 Fragments arranged by size: 1272 652 425 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 394 424 587 617 934 964 1663 1693 Size: 394 30 163 30 317 30 699 30 Cuts at: 1693 2179 2209 2349 Size: 486 30 140 Fragments arranged by size: 699 486 394 317 163 140 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 457 549 982 985 1870 2158 2161 2200 Size: 457 92 433 3 885 288 3 39 Cuts at: 2200 2349 Size: 149 Fragments arranged by size: 885 457 433 288 149 92 39 3 3 BseYI C'CCAG_C Cuts at: 0 43 611 823 955 999 1174 1248 1522 Size: 43 568 212 132 44 175 74 274 Cuts at: 1522 1702 1903 1997 2215 2349 Size: 180 201 94 218 134 Fragments arranged by size: 568 274 218 212 201 180 175 134 132 94 74 44 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 1168 1828 1899 2349 Size: 223 945 660 71 450 Fragments arranged by size: 945 660 450 223 71 BsiEI CG_ry'CG Cuts at: 0 154 233 2349 Size: 154 79 2116 Fragments arranged by size: 2116 154 79 BsiHKAI G_wGCw'C Cuts at: 0 407 559 874 1322 2349 Size: 407 152 315 448 1027 Fragments arranged by size: 1027 448 407 315 152 BsmBI CGTCTCn'nnnn_ Cuts at: 0 354 390 2349 Size: 354 36 1959 Fragments arranged by size: 1959 354 36 Bsp1286I G_dGCh'C Cuts at: 0 31 42 71 116 260 376 400 407 Size: 31 11 29 45 144 116 24 7 Cuts at: 407 559 589 652 704 874 1000 1181 1187 Size: 152 30 63 52 170 126 181 6 Cuts at: 1187 1227 1255 1322 1422 1429 1703 1804 1940 Size: 40 28 67 100 7 274 101 136 Cuts at: 1940 2064 2349 Size: 124 285 Fragments arranged by size: 285 274 181 170 152 144 136 126 124 116 101 100 67 63 52 45 40 31 30 29 28 24 11 7 7 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 786 1077 2349 Size: 786 291 1272 Fragments arranged by size: 1272 786 291 BsrBI CCG'CTC Cuts at: 0 837 1083 2349 Size: 837 246 1266 Fragments arranged by size: 1266 837 246 BsrDI GCAATG_nn' Cuts at: 0 1303 1308 2349 Size: 1303 5 1041 Fragments arranged by size: 1303 1041 5 BsrFI r'CCGG_y Cuts at: 0 1361 2349 Size: 1361 988 BsrGI T'GTAC_A Cuts at: 0 2024 2349 Size: 2024 325 BssSI C'ACGA_G Cuts at: 0 274 2349 Size: 274 2075 BstAPI GCAn_nnn'nTGC Cuts at: 0 1913 2349 Size: 1913 436 BstXI CCAn_nnnn'nTGG Cuts at: 0 1929 2206 2349 Size: 1929 277 143 Fragments arranged by size: 1929 277 143 BstYI r'GATC_y Cuts at: 0 1899 2349 Size: 1899 450 Bsu36I CC'TnA_GG Cuts at: 0 1576 2349 Size: 1576 773 BtgI C'CryG_G Cuts at: 0 1133 2349 Size: 1133 1216 BtsI GCAGTG_nn' Cuts at: 0 235 906 1863 1914 2160 2349 Size: 235 671 957 51 246 189 Fragments arranged by size: 957 671 246 235 189 51 EaeI y'GGCC_r Cuts at: 0 151 230 1931 2349 Size: 151 79 1701 418 Fragments arranged by size: 1701 418 151 79 EagI C'GGCC_G Cuts at: 0 151 230 2349 Size: 151 79 2119 Fragments arranged by size: 2119 151 79 EarI CTCTTCn'nnn_ Cuts at: 0 2023 2349 Size: 2023 326 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 905 1396 2349 Size: 905 491 953 Fragments arranged by size: 953 905 491 EcoICRI GAG'CTC Cuts at: 0 405 557 872 2349 Size: 405 152 315 1477 Fragments arranged by size: 1477 405 315 152 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 56 336 347 388 541 573 614 Size: 32 24 280 11 41 153 32 41 Cuts at: 614 637 744 1315 1690 1717 1736 2288 2349 Size: 23 107 571 375 27 19 552 61 Fragments arranged by size: 571 552 375 280 153 107 61 41 41 32 32 27 24 23 19 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 934 1396 1777 2026 2349 Size: 934 462 381 249 323 Fragments arranged by size: 934 462 381 323 249 EcoO109I rG'GnC_Cy Cuts at: 0 96 306 585 693 700 986 1152 1252 Size: 96 210 279 108 7 286 166 100 Cuts at: 1252 1401 1425 1426 1442 1580 2060 2061 2296 Size: 149 24 1 16 138 480 1 235 Cuts at: 2296 2349 Size: 53 Fragments arranged by size: 480 286 279 235 210 166 149 138 108 100 96 53 24 16 7 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 747 2349 Size: 747 1602 HaeII r_GCGC'y Cuts at: 0 183 1055 1216 1362 1529 1824 1848 2349 Size: 183 872 161 146 167 295 24 501 Fragments arranged by size: 872 501 295 183 167 161 146 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 934 966 1669 1701 2349 Size: 934 32 703 32 648 Fragments arranged by size: 934 703 648 32 32 HindIII A'AGCT_T Cuts at: 0 729 2349 Size: 729 1620 KasI G'GCGC_C Cuts at: 0 1358 2349 Size: 1358 991 KpnI G_GTAC'C Cuts at: 0 885 2349 Size: 885 1464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1454 1667 2349 Size: 1454 213 682 Fragments arranged by size: 1454 682 213 MspA1I CmG'CkG Cuts at: 0 125 150 213 229 474 582 615 855 Size: 125 25 63 16 245 108 33 240 Cuts at: 855 1135 1143 1503 2349 Size: 280 8 360 846 Fragments arranged by size: 846 360 280 245 240 125 108 63 33 25 16 8 NaeI GCC'GGC Cuts at: 0 1363 2349 Size: 1363 986 NarI GG'CG_CC Cuts at: 0 1359 2349 Size: 1359 990 NgoMIV G'CCGG_C Cuts at: 0 1361 2349 Size: 1361 988 NotI GC'GGCC_GC Cuts at: 0 230 2349 Size: 230 2119 NspI r_CATG'y Cuts at: 0 246 318 1291 1686 2349 Size: 246 72 973 395 663 Fragments arranged by size: 973 663 395 246 72 PasI CC'CwG_GG Cuts at: 0 1383 1422 2056 2127 2234 2349 Size: 1383 39 634 71 107 115 Fragments arranged by size: 1383 634 115 107 71 39 PciI A'CATG_T Cuts at: 0 1287 1682 2349 Size: 1287 395 667 Fragments arranged by size: 1287 667 395 PflMI CCAn_nnn'nTGG Cuts at: 0 939 2349 Size: 939 1410 PfoI T'CCnGG_A Cuts at: 0 33 365 1292 2349 Size: 33 332 927 1057 Fragments arranged by size: 1057 927 332 33 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 394 426 2349 Size: 394 32 1923 Fragments arranged by size: 1923 394 32 PpuMI rG'GwC_Cy Cuts at: 0 96 693 986 1152 1442 1580 2349 Size: 96 597 293 166 290 138 769 Fragments arranged by size: 769 597 293 290 166 138 96 PspOMI G'GGCC_C Cuts at: 0 585 700 1177 1223 1251 1418 1425 2060 Size: 585 115 477 46 28 167 7 635 Cuts at: 2060 2349 Size: 289 Fragments arranged by size: 635 585 477 289 167 115 46 28 7 PstI C_TGCA'G Cuts at: 0 212 479 1199 1502 1550 2349 Size: 212 267 720 303 48 799 Fragments arranged by size: 799 720 303 267 212 48 PvuII CAG'CTG Cuts at: 0 213 615 855 1143 1503 2349 Size: 213 402 240 288 360 846 Fragments arranged by size: 846 402 360 288 240 213 SacI G_AGCT'C Cuts at: 0 407 559 874 2349 Size: 407 152 315 1475 Fragments arranged by size: 1475 407 315 152 SacII CC_GC'GG Cuts at: 0 1136 2349 Size: 1136 1213 SanDI GG'GwC_CC Cuts at: 0 986 1442 2349 Size: 986 456 907 Fragments arranged by size: 986 907 456 SbfI CC_TGCA'GG Cuts at: 0 1550 2349 Size: 1550 799 SfcI C'TryA_G Cuts at: 0 208 475 1195 1498 1546 2349 Size: 208 267 720 303 48 803 Fragments arranged by size: 803 720 303 267 208 48 SfiI GGCCn_nnn'nGGCC Cuts at: 0 150 2349 Size: 150 2199 SfoI GGC'GCC Cuts at: 0 1360 2349 Size: 1360 989 SmaI CCC'GGG Cuts at: 0 263 454 689 991 1228 1417 2349 Size: 263 191 235 302 237 189 932 Fragments arranged by size: 932 302 263 237 235 191 189 SmlI C'TyrA_G Cuts at: 0 632 1321 2349 Size: 632 689 1028 Fragments arranged by size: 1028 689 632 SphI G_CATG'C Cuts at: 0 318 2349 Size: 318 2031 SrfI GCCC'GGGC Cuts at: 0 1228 1417 2349 Size: 1228 189 932 Fragments arranged by size: 1228 932 189 StuI AGG'CCT Cuts at: 0 1552 1948 2349 Size: 1552 396 401 Fragments arranged by size: 1552 401 396 StyI C'CwwG_G Cuts at: 0 389 696 2349 Size: 389 307 1653 Fragments arranged by size: 1653 389 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 268 1031 1136 1536 1876 2349 Size: 268 763 105 400 340 473 Fragments arranged by size: 763 473 400 340 268 105 TatI w'GTAC_w Cuts at: 0 1679 2024 2349 Size: 1679 345 325 Fragments arranged by size: 1679 345 325 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 587 619 2179 2211 2349 Size: 587 32 1560 32 138 Fragments arranged by size: 1560 587 138 32 32 Tth111I GACn'n_nGTC Cuts at: 0 421 2349 Size: 421 1928 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 534 1171 2349 Size: 534 637 1178 Fragments arranged by size: 1178 637 534 XmaI C'CCGG_G Cuts at: 0 261 452 687 989 1226 1415 2349 Size: 261 191 235 302 237 189 934 Fragments arranged by size: 934 302 261 237 235 191 189 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI KpnI MmeI MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AhdI AleI ApaLI AscI AseI AsiSI BaeI BbsI BcgI BciVI BclI BfrBI BglII BmtI BsaAI BsaBI BsiWI BsmI BspEI BspHI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HincII HpaI MfeI MluI MscI MslI NcoI NdeI NheI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI XmnI ZraI