################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbeI BbrPI BciVI BfrI BglI BlnI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoRI EcoRV EcoT22I HaeII HindIII HpaI KasI KpnI MamI MfeI MroI MseI MunI NarI NcoI NdeI NheI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tru9I XbaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AlwNI 328 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 1668 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 1751 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1259 TGATCA T^GATCA BfaI 137 CTAG C^TAG BlpI 478 GCTGAGC GC^T[ACGT]AGC Bpu1102I 478 GCTGAGC GC^T[ACGT]AGC BsaAI 772 TACGTG [CT]AC^GT[GA] BsmBI 1446 CGTCTC CGTCTC(1/5) BsrDI 1265 GCAATG GCAATG(2/0) BstAPI 1946 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 478 GCTGAGC GC^T[ACGT]AGC EarI 199 CTCTTC CTCTTC(1/4) FbaI 1259 TGATCA T^GATCA FseI 636 GGCCGGCC GGCCGG^CC HincII 397 GTCAAC GT[CT]^[GA]AC HindII 397 GTCAAC GT[CT]^[GA]AC Ksp632I 199 CTCTTC CTCTTC(1/4) KspI 6 CCGCGG CCGC^GG MaeI 137 CTAG C^TAG MaeII 773 ACGT A^CGT MluI 1849 ACGCGT A^CGCGT MslI 1239 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 637 GCCGGC GCC^GGC NgoMIV 637 GCCGGC G^CCGGC NotI 588 GCGGCCGC GC^GGCCGC PaeR7I 580 CTCGAG C^TCGAG PflFI 1668 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 240 GACTC GAGTC(4/5) SacI 1293 GAGCTC GAGCT^C SacII 6 CCGCGG CCGC^GG SbfI 756 CCTGCAGG CCTGCA^GG Sse8387I 756 CCTGCAGG CCTGCA^GG TfiI 338 GAATC G^A[AT]TC Tsp509I 361 AATT ^AATT Tth111I 1668 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 580 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 790 GACGCC G[GA]^CG[CT]C 1096 GACGCC AflIII 773 ACGTGT A^C[GA][CT]GT 1849 ACGCGT Alw44I 1143 GTGCAC G^TGCAC 1318 GTGCAC AocI 660 CCTGAGG CC^T[ACGT]AGG 1495 CCTGAGG ApaLI 1143 GTGCAC G^TGCAC 1318 GTGCAC BalI 41 TGGCCA TGG^CCA 956 TGGCCA BbsI 313 GTCTTC GAAGAC(2/6) 1702 GTCTTC BglII 809 AGATCT A^GATCT 839 AGATCT BpuAI 313 GTCTTC GAAGAC(2/6) 1702 GTCTTC BsaHI 790 GACGCC G[GA]^CG[CT]C 1096 GACGCC BsaI 144 GGTCTC GGTCTC(1/5) 1673 GGTCTC BsiEI 589 CGGCCG CG[GA][CT]^CG 683 CGGCCG BspMI 1242 GCAGGT ACCTGC(4/8) 1425 GCAGGT BsrFI 637 GCCGGC [GA]^CCGG[CT] 1630 ACCGGC BstEII 372 GGTCACC G^GT[ACGT]ACC 1576 GGTGACC BstPI 372 GGTCACC G^GT[ACGT]ACC 1576 GGTGACC Bsu36I 660 CCTGAGG CC^T[ACGT]AGG 1495 CCTGAGG Cfr10I 637 GCCGGC [GA]^CCGG[CT] 1630 ACCGGC DrdI 1201 GACAAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1681 GACCAGGAAGTC EagI 589 CGGCCG C^GGCCG 683 CGGCCG EclXI 589 CGGCCG C^GGCCG 683 CGGCCG Eco52I 589 CGGCCG C^GGCCG 683 CGGCCG Eco81I 660 CCTGAGG CC^T[ACGT]AGG 1495 CCTGAGG EcoNI 897 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1495 CCTGAGGGAGG EcoO65I 372 GGTCACC G^GT[ACGT]ACC 1576 GGTGACC Hin1I 790 GACGCC G[GA]^CG[CT]C 1096 GACGCC HinfI 240 GACTC G^A[ACGT]TC 338 GAATC MluNI 41 TGGCCA TGG^CCA 956 TGGCCA MscI 41 TGGCCA TGG^CCA 956 TGGCCA PflMI 327 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 561 CCAGGACATGG SmaI 390 CCCGGG CCC^GGG 730 CCCGGG SphI 545 GCATGC GCATG^C 1238 GCATGC Van91I 327 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 561 CCAGGACATGG XcmI 557 CCAGCCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1958 CCACCAGCAGCATGG XmaCI 390 CCCGGG C^CCGGG 730 CCCGGG XmaI 390 CCCGGG C^CCGGG 730 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AspHI AvaI AvaII AviII BanI BanII BbvCI BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII