(Linear) (Six-base) MAPSORT of: KIEE1380.seq Check: 5236 from: 1 to: 1974 KIEE1380.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1233 1974 Size: 1233 741 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1572 1974 Size: 79 1493 402 Fragments arranged by size: 1493 402 79 AflIII A'CryG_T Cuts at: 0 773 1849 1974 Size: 773 1076 125 Fragments arranged by size: 1076 773 125 AlwNI CAG_nnn'CTG Cuts at: 0 333 1974 Size: 333 1641 ApaI G_GGCC'C Cuts at: 0 109 223 463 692 1812 1974 Size: 109 114 240 229 1120 162 Fragments arranged by size: 1120 240 229 162 114 109 ApaLI G'TGCA_C Cuts at: 0 1143 1318 1974 Size: 1143 175 656 Fragments arranged by size: 1143 656 175 AvaI C'yCGr_G Cuts at: 0 109 275 390 580 730 1570 1974 Size: 109 166 115 190 150 840 404 Fragments arranged by size: 840 404 190 166 150 115 109 BanI G'GyrC_C Cuts at: 0 265 1504 1633 1792 1974 Size: 265 1239 129 159 182 Fragments arranged by size: 1239 265 182 159 129 BanII G_rGCy'C Cuts at: 0 109 223 389 463 692 1297 1812 1874 Size: 109 114 166 74 229 605 515 62 Cuts at: 1874 1974 Size: 100 Fragments arranged by size: 605 515 229 166 114 109 100 74 62 BbsI GAAGACnn'nnnn_ Cuts at: 0 306 1695 1974 Size: 306 1389 279 Fragments arranged by size: 1389 306 279 BbvCI CC'TCA_GC Cuts at: 0 55 1366 1639 1974 Size: 55 1311 273 335 Fragments arranged by size: 1311 335 273 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1740 1774 1974 Size: 1740 34 200 Fragments arranged by size: 1740 200 34 BclI T'GATC_A Cuts at: 0 1259 1974 Size: 1259 715 BglII A'GATC_T Cuts at: 0 809 839 1974 Size: 809 30 1135 Fragments arranged by size: 1135 809 30 BlpI GC'TnA_GC Cuts at: 0 479 1974 Size: 479 1495 Bme1580I G_kGCm'C Cuts at: 0 109 223 463 692 1147 1322 1812 1974 Size: 109 114 240 229 455 175 490 162 Fragments arranged by size: 490 455 240 229 175 162 114 109 BmrI ACTGGGnnnn_n' Cuts at: 0 523 1974 Size: 523 1451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 229 253 310 584 842 966 1212 Size: 226 3 24 57 274 258 124 246 Cuts at: 1212 1366 1447 1974 Size: 154 81 527 Fragments arranged by size: 527 274 258 246 226 154 124 81 57 24 3 Bpu10I CC'TnA_GC Cuts at: 0 55 164 1366 1639 1974 Size: 55 109 1202 273 335 Fragments arranged by size: 1202 335 273 109 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 872 1661 1974 Size: 460 412 789 313 Fragments arranged by size: 789 460 412 313 BsaI GGTCTCn'nnnn_ Cuts at: 0 150 1679 1974 Size: 150 1529 295 Fragments arranged by size: 1529 295 150 BsaAI yAC'GTr Cuts at: 0 774 1974 Size: 774 1200 BsaHI Gr'CG_yC Cuts at: 0 791 1097 1974 Size: 791 306 877 Fragments arranged by size: 877 791 306 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1017 1047 1364 1394 1493 1523 1974 Size: 1017 30 317 30 99 30 451 Fragments arranged by size: 1017 451 317 99 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 496 1022 1588 1890 1974 Size: 496 526 566 302 84 Fragments arranged by size: 566 526 496 302 84 BseYI C'CCAG_C Cuts at: 0 1174 1353 1776 1858 1974 Size: 1174 179 423 82 116 Fragments arranged by size: 1174 423 179 116 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 1444 1629 1808 1974 Size: 331 1113 185 179 166 Fragments arranged by size: 1113 331 185 179 166 BsiEI CG_ry'CG Cuts at: 0 592 686 1974 Size: 592 94 1288 Fragments arranged by size: 1288 592 94 BsiHKAI G_wGCw'C Cuts at: 0 1147 1297 1322 1939 1948 1974 Size: 1147 150 25 617 9 26 Fragments arranged by size: 1147 617 150 26 25 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1452 1974 Size: 1452 522 Bsp1286I G_dGCh'C Cuts at: 0 109 223 389 463 692 1147 1297 1322 Size: 109 114 166 74 229 455 150 25 Cuts at: 1322 1812 1874 1939 1948 1974 Size: 490 62 65 9 26 Fragments arranged by size: 490 455 229 166 150 114 109 74 65 62 26 25 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1233 1416 1974 Size: 1233 183 558 Fragments arranged by size: 1233 558 183 BsrDI GCAATG_nn' Cuts at: 0 1272 1974 Size: 1272 702 BsrFI r'CCGG_y Cuts at: 0 637 1630 1974 Size: 637 993 344 Fragments arranged by size: 993 637 344 BstAPI GCAn_nnn'nTGC Cuts at: 0 1952 1974 Size: 1952 22 BstEII G'GTnAC_C Cuts at: 0 372 1576 1974 Size: 372 1204 398 Fragments arranged by size: 1204 398 372 BstXI CCAn_nnnn'nTGG Cuts at: 0 930 1107 1968 1974 Size: 930 177 861 6 Fragments arranged by size: 930 861 177 6 BstYI r'GATC_y Cuts at: 0 434 735 809 839 1974 Size: 434 301 74 30 1135 Fragments arranged by size: 1135 434 301 74 30 Bsu36I CC'TnA_GG Cuts at: 0 661 1496 1974 Size: 661 835 478 Fragments arranged by size: 835 661 478 BtgI C'CryG_G Cuts at: 0 6 1508 1874 1974 Size: 6 1502 366 100 Fragments arranged by size: 1502 366 100 6 BtsI GCAGTG_nn' Cuts at: 0 315 343 1224 1320 1817 1974 Size: 315 28 881 96 497 157 Fragments arranged by size: 881 497 315 157 96 28 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 51 86 332 367 1974 Size: 51 35 246 35 1607 Fragments arranged by size: 1607 246 51 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1207 1687 1974 Size: 1207 480 287 Fragments arranged by size: 1207 480 287 EaeI y'GGCC_r Cuts at: 0 41 589 635 639 683 956 1532 1974 Size: 41 548 46 4 44 273 576 442 Fragments arranged by size: 576 548 442 273 46 44 41 4 EagI C'GGCC_G Cuts at: 0 589 683 1974 Size: 589 94 1291 Fragments arranged by size: 1291 589 94 EarI CTCTTCn'nnn_ Cuts at: 0 205 1974 Size: 205 1769 EcoICRI GAG'CTC Cuts at: 0 1295 1974 Size: 1295 679 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 226 229 253 310 584 842 966 Size: 79 147 3 24 57 274 258 124 Cuts at: 966 1212 1366 1447 1572 1974 Size: 246 154 81 125 402 Fragments arranged by size: 402 274 258 246 154 147 125 124 81 79 57 24 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 901 1499 1974 Size: 901 598 475 Fragments arranged by size: 901 598 475 EcoO109I rG'GnC_Cy Cuts at: 0 106 168 330 459 460 666 689 763 Size: 106 62 162 129 1 206 23 74 Cuts at: 763 1170 1492 1808 1809 1974 Size: 407 322 316 1 165 Fragments arranged by size: 407 322 316 206 165 162 129 106 74 62 23 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1227 1974 Size: 1227 747 FseI GG_CCGG'CC Cuts at: 0 641 1974 Size: 641 1333 FspI TGC'GCA Cuts at: 0 726 1133 1216 1974 Size: 726 407 83 758 Fragments arranged by size: 758 726 407 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 400 432 1017 1049 1327 1359 1659 1691 Size: 400 32 585 32 278 32 300 32 Cuts at: 1691 1974 Size: 283 Fragments arranged by size: 585 400 300 283 278 32 32 32 32 HincII GTy'rAC Cuts at: 0 399 1974 Size: 399 1575 MluI A'CGCG_T Cuts at: 0 1849 1974 Size: 1849 125 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 534 1415 1974 Size: 534 881 559 Fragments arranged by size: 881 559 534 MscI TGG'CCA Cuts at: 0 43 958 1974 Size: 43 915 1016 Fragments arranged by size: 1016 915 43 MslI CAynn'nnrTG Cuts at: 0 1243 1974 Size: 1243 731 MspA1I CmG'CkG Cuts at: 0 8 47 231 451 954 986 1178 1232 Size: 8 39 184 220 503 32 192 54 Cuts at: 1232 1308 1353 1475 1643 1974 Size: 76 45 122 168 331 Fragments arranged by size: 503 331 220 192 184 168 122 76 54 45 39 32 8 NaeI GCC'GGC Cuts at: 0 639 1974 Size: 639 1335 NgoMIV G'CCGG_C Cuts at: 0 637 1974 Size: 637 1337 NotI GC'GGCC_GC Cuts at: 0 589 1974 Size: 589 1385 NspI r_CATG'y Cuts at: 0 549 807 1242 1974 Size: 549 258 435 732 Fragments arranged by size: 732 549 435 258 PasI CC'CwG_GG Cuts at: 0 333 525 1974 Size: 333 192 1449 Fragments arranged by size: 1449 333 192 PflMI CCAn_nnn'nTGG Cuts at: 0 333 567 1974 Size: 333 234 1407 Fragments arranged by size: 1407 333 234 PfoI T'CCnGG_A Cuts at: 0 203 1974 Size: 203 1771 PpuMI rG'GwC_Cy Cuts at: 0 666 763 1170 1974 Size: 666 97 407 804 Fragments arranged by size: 804 666 407 97 PspOMI G'GGCC_C Cuts at: 0 105 219 459 688 1808 1974 Size: 105 114 240 229 1120 166 Fragments arranged by size: 1120 240 229 166 114 105 PspXI vC'TCGA_Gb Cuts at: 0 580 1974 Size: 580 1394 PstI C_TGCA'G Cuts at: 0 52 761 1004 1974 Size: 52 709 243 970 Fragments arranged by size: 970 709 243 52 PvuII CAG'CTG Cuts at: 0 47 231 451 986 1178 1232 1353 1475 Size: 47 184 220 535 192 54 121 122 Cuts at: 1475 1643 1974 Size: 168 331 Fragments arranged by size: 535 331 220 192 184 168 122 121 54 47 SacI G_AGCT'C Cuts at: 0 1297 1974 Size: 1297 677 SacII CC_GC'GG Cuts at: 0 9 1974 Size: 9 1965 SanDI GG'GwC_CC Cuts at: 0 763 1170 1974 Size: 763 407 804 Fragments arranged by size: 804 763 407 SbfI CC_TGCA'GG Cuts at: 0 761 1974 Size: 761 1213 SfcI C'TryA_G Cuts at: 0 48 757 1000 1974 Size: 48 709 243 974 Fragments arranged by size: 974 709 243 48 SmaI CCC'GGG Cuts at: 0 392 732 1974 Size: 392 340 1242 Fragments arranged by size: 1242 392 340 SmlI C'TyrA_G Cuts at: 0 439 580 887 1676 1974 Size: 439 141 307 789 298 Fragments arranged by size: 789 439 307 298 141 SphI G_CATG'C Cuts at: 0 549 1242 1974 Size: 549 693 732 Fragments arranged by size: 732 693 549 StuI AGG'CCT Cuts at: 0 893 1387 1757 1974 Size: 893 494 370 217 Fragments arranged by size: 893 494 370 217 StyI C'CwwG_G Cuts at: 0 605 1037 1535 1974 Size: 605 432 498 439 Fragments arranged by size: 605 498 439 432 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1954 1974 Size: 1954 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1364 1396 1493 1525 1837 1869 1974 Size: 1364 32 97 32 312 32 105 Fragments arranged by size: 1364 312 105 97 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1671 1974 Size: 1671 303 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 564 1965 1974 Size: 564 1401 9 Fragments arranged by size: 1401 564 9 XhoI C'TCGA_G Cuts at: 0 580 1974 Size: 580 1394 XmaI C'CCGG_G Cuts at: 0 390 730 1974 Size: 390 340 1244 Fragments arranged by size: 1244 390 340 Enzymes that do cut: AarI AcuI AflIII AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BclI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI Hin4I HincII MluI MmeI MscI MslI MspA1I NaeI NgoMIV NotI NspI PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SbfI SfcI SmaI SmlI SphI StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglI BmgBI BmtI BplI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI DraIII EciI EcoRI EcoRV FspAI HaeII HindIII HpaI KasI KpnI MfeI NarI NcoI NdeI NheI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI XbaI XmnI ZraI