################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI Asp700I AspEI AspI AvaI AvaII AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrI BssHII BstBI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1142 GGTACC G^GTACC AccI 184 GTATAC GT^[AC][GT]AC Alw44I 365 GTGCAC G^TGCAC AlwNI 518 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 365 GTGCAC G^TGCAC Asp718I 1142 GGTACC G^GTACC AspHI 365 GTGCAC G[AT]GC[AT]^C BamHI 583 GGATCC G^GATCC BanI 1142 GGTACC G^G[CT][GA]CC BbvI 531 GCAGC GCAGC(8/12) BfaI 571 CTAG C^TAG BmyI 365 GTGCAC G[AGT]GC[ACT]^C BpmI 941 CTCCAG CTGGAG(16/14) BsaWI 985 ACCGGA [AT]^CCGG[AT] BsiHKAI 365 GTGCAC G[AT]GC[AT]^C BsmBI 1181 GAGACG CGTCTC(1/5) BsmFI 548 GGGAC GGGAC(10/14) Bsp1286I 365 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 132 TGTACA T^GTACA BsrDI 901 CATTGC GCAATG(2/0) BsrGI 132 TGTACA T^GTACA Bst1107I 184 GTATAC GTA^TAC BstAPI 517 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 60 GGATG GGATG(2/0) BstZ17I 184 GTATAC GTA^TAC Cac8I 1013 GCATGC GC[ACGT]^[ACGT]GC EarI 916 GAAGAG CTCTTC(1/4) EcoRV 3 GATATC GAT^ATC Fnu4HI 531 GCAGC GC^[ACGT]GC FokI 60 GGATG GGATG(9/13) HapII 986 CCGG C^CGG HincII 229 GTCAAC GT[CT]^[GA]AC HindII 229 GTCAAC GT[CT]^[GA]AC HpaII 986 CCGG C^CGG HphI 679 GGTGA GGTGA(8/7) ItaI 531 GCAGC GC^[ACGT]GC KpnI 1142 GGTACC GGTAC^C Ksp632I 916 GAAGAG CTCTTC(1/4) MaeI 571 CTAG C^TAG MfeI 197 CAATTG C^AATTG MslI 496 CATAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 986 CCGG C^CGG MunI 197 CAATTG C^AATTG NspI 1013 GCATGC [GA]CATG^[CT] PflMI 1158 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 143 GACTC GAGTC(4/5) PvuII 532 CAGCTG CAG^CTG ScaI 800 AGTACT AGT^ACT SfaNI 76 GCATC GCATC(5/9) SfcI 848 CTGTAG C^T[GA][CT]AG SphI 1013 GCATGC GCATG^C SspBI 132 TGTACA T^GTACA TaqI 1007 TCGA T^CGA TseI 531 GCAGC G^C[AT]GC TspRI 417 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 1007 TCGA T^CGA Van91I 1158 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AseI 259 ATTAAT AT^TAAT 756 ATTAAT AsnI 259 ATTAAT AT^TAAT 756 ATTAAT BclI 11 TGATCA T^GATCA 627 TGATCA BsaI 982 GAGACC GGTCTC(1/5) 1097 GGTCTC BsgI 406 CTGCAC GTGCAG(16/14) 529 GTGCAG BssSI 38 CACGAG CACGAG(-5/-1) 92 CACGAG BstYI 423 GGATCT [GA]^GATC[CT] 583 GGATCC DdeI 607 CTAAG C^T[ACGT]AG 1100 CTCAG DraI 595 TTTAAA TTT^AAA 651 TTTAAA FbaI 11 TGATCA T^GATCA 627 TGATCA MflI 423 GGATCT [GA]^GATC[CT] 583 GGATCC MspA1I 532 CAGCTG C[AC]G^C[GT]G 587 CCGCTG NlaIII 997 CATG CATG^ 1014 CATG PshBI 259 ATTAAT AT^TAAT 756 ATTAAT TfiI 341 GATTC G^A[AT]TC 689 GATTC XhoII 423 GGATCT [GA]^GATC[CT] 583 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BsaJI BseRI BsiYI BslI BsmAI BssKI BstNI DpnII EcoRII EcoT14I HinfI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV RsaI Sau3AI ScrFI StyI Tru9I Tsp45I Tsp509I