(Circular) (Six-base) MAPSORT of: pFN21AE4383.seq Check: 342 from: 1 to: 5917 pFN21AE4383 5917 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2190 3260 1937 Size: 253 1070 4594 Fragments arranged by size: 4594 1070 253 Acc65I G'GTAC_C Cuts at: 3147 3238 3147 Size: 91 5826 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2742 3980 5364 5632 1632 Size: 1110 1238 1384 268 1917 Fragments arranged by size: 1917 1384 1238 1110 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 4816 5793 1203 Size: 3613 977 1327 Fragments arranged by size: 3613 1327 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2528 3660 5232 2528 Size: 1132 1572 3213 Fragments arranged by size: 3213 1572 1132 ApaLI G'TGCA_C Cuts at: 2370 3974 5130 2370 Size: 1604 1156 3157 Fragments arranged by size: 3157 1604 1156 ApoI r'AATT_y Cuts at: 1493 2294 2417 2741 2859 2931 3056 3194 3226 Size: 801 123 324 118 72 125 138 32 Cuts at: 3226 3452 3604 5713 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 801 324 226 152 138 125 123 118 72 32 AseI AT'TA_AT Cuts at: 160 1032 2265 2762 4471 160 Size: 872 1233 497 1709 1606 Fragments arranged by size: 1709 1606 1233 872 497 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 3242 1870 Size: 73 1299 4545 Fragments arranged by size: 4545 1299 73 BamHI G'GATC_C Cuts at: 2588 3247 3614 2588 Size: 659 367 4891 Fragments arranged by size: 4891 659 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3147 3238 4693 618 Size: 325 217 627 1360 91 1455 1842 Fragments arranged by size: 1842 1455 1360 627 325 217 91 BanII G_rGCy'C Cuts at: 1391 3236 1391 Size: 1845 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 2016 2632 1548 Size: 468 616 4833 Fragments arranged by size: 4833 616 468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5912 5912 Size: 5917 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 2374 3978 5134 1790 Size: 584 1604 1156 2573 Fragments arranged by size: 2573 1604 1156 584 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2931 4577 1239 Size: 1692 1646 2579 Fragments arranged by size: 2579 1692 1646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4042 4907 5205 4042 Size: 865 298 4754 Fragments arranged by size: 4754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2981 3108 4580 882 Size: 617 1482 127 1472 2219 Fragments arranged by size: 2219 1482 1472 617 127 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2990 4350 4748 5022 5169 5580 2990 Size: 1360 398 274 147 411 3327 Fragments arranged by size: 3327 1360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2466 2939 3198 1186 Size: 1280 473 259 3905 Fragments arranged by size: 3905 1280 473 259 BseYI C'CCAG_C Cuts at: 5120 5616 5120 Size: 496 5421 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2396 2555 1163 Size: 573 660 159 4525 Fragments arranged by size: 4525 660 573 159 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2374 3978 4063 5134 2374 Size: 1604 85 1071 3157 Fragments arranged by size: 3157 1604 1071 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 BsmBI CGTCTCn'nnnn_ Cuts at: 3180 3180 Size: 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2374 3236 3978 4063 5134 1391 Size: 399 584 862 742 85 1071 2174 Fragments arranged by size: 2174 1071 862 742 584 399 85 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3272 844 Size: 2428 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2905 4412 4586 65 Size: 1183 1657 1507 174 1396 Fragments arranged by size: 1657 1507 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 2137 96 Size: 2041 3876 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 2043 2097 3977 4989 2043 Size: 54 1880 1012 2971 Fragments arranged by size: 2971 1880 1012 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 2528 794 Size: 1734 4183 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2428 2588 3247 3614 3999 4016 5468 5554 Size: 456 160 659 367 385 17 1452 86 Cuts at: 5554 5566 5912 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 659 456 385 367 346 160 86 17 12 BstZ17I GTA'TAC Cuts at: 2191 2191 Size: 5917 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5365 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 3433 4227 4247 3433 Size: 794 20 5103 Fragments arranged by size: 5103 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 2602 2658 3221 2602 Size: 56 563 5298 Fragments arranged by size: 5298 563 56 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3364 4253 5882 Size: 54 1425 50 275 18 1534 889 1629 Cuts at: 5882 8 Size: 43 Fragments arranged by size: 1629 1534 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2916 3847 1353 Size: 1563 931 3423 Fragments arranged by size: 3423 1563 931 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2742 2931 3980 4577 5364 5632 1239 Size: 393 1110 189 1049 597 787 268 1524 Fragments arranged by size: 1524 1110 1049 787 597 393 268 189 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 EcoRV GAT'ATC Cuts at: 2010 2010 Size: 5917 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 5064 1678 Size: 3386 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 4558 4590 4632 Size: 32 135 32 436 32 2518 32 42 Cuts at: 4632 4664 1373 Size: 32 2626 Fragments arranged by size: 2626 2518 436 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 3261 3507 669 Size: 988 281 298 1025 246 3079 Fragments arranged by size: 3079 1025 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 3151 3242 3151 Size: 91 5826 MfeI C'AATT_G Cuts at: 2202 3516 2202 Size: 1314 4603 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2757 3009 3051 5031 5215 1305 Size: 1452 252 42 1980 184 2007 Fragments arranged by size: 2007 1980 1452 252 184 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4095 Fragments arranged by size: 4095 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2505 3875 4234 4393 518 Size: 648 1339 1370 359 159 2042 Fragments arranged by size: 2042 1370 1339 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2539 2594 3333 4010 5158 5403 1606 Size: 933 55 739 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 739 677 245 55 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5365 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 3022 3275 4820 1132 Size: 97 1793 253 1545 2229 Fragments arranged by size: 2229 1793 1545 253 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 3169 1108 Size: 2061 3856 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 2160 3487 2160 Size: 1327 4590 PstI C_TGCA'G Cuts at: 830 3269 830 Size: 2439 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2539 2539 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 2807 4165 5877 2807 Size: 1358 1712 2847 Fragments arranged by size: 2847 1712 1358 SfcI C'TryA_G Cuts at: 826 1045 2853 3265 4400 5081 5272 826 Size: 219 1808 412 1135 681 191 1471 Fragments arranged by size: 1808 1471 1135 681 412 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4021 4922 5184 820 Size: 197 926 2078 901 262 1553 Fragments arranged by size: 2078 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3022 3275 1132 Size: 1890 253 3774 Fragments arranged by size: 3774 1890 253 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 2389 2911 3178 3356 513 Size: 552 1324 522 267 178 3074 Fragments arranged by size: 3074 1324 552 522 267 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2137 2805 4163 Size: 274 80 33 51 1443 160 668 1358 Cuts at: 4163 5875 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1358 668 274 160 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI