Restriction map for
pFN21AB5751
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BamHI BbeI BcgI BciVI BfrI
BglII BlnI BsaBI BsaI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstZ17I Cfr10I ClaI
DraI DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FseI FspI HpaI
KasI MamI MfeI MluI MslI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI SacI SalI
SbfI SfiI SfoI SgrAI SmlI
SnaBI SphI Sse8387I SspBI SwaI
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 2086 GTAGAC GT^[AC][GT]AC
AcyI 166 GGCGTC G[GA]^CG[CT]C
AflIII 1052 ACACGT A^C[GA][CT]GT
Alw44I 946 GTGCAC G^TGCAC
ApaI 2039 GGGCCC GGGCC^C
ApaLI 946 GTGCAC G^TGCAC
AspI 163 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 943 CACGTG CAC^GTG
BbsI 1961 GTCTTC GAAGAC(2/6)
BbvCI 2286 CCTCAGC CCTCAGC(-5/-2)
BclI 1175 TGATCA T^GATCA
BglI 1279 GCCATTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1961 GTCTTC GAAGAC(2/6)
BsaAI 943 CACGTG [CT]AC^GT[GA]
BsaHI 166 GGCGTC G[GA]^CG[CT]C
BsiEI 150 CGGCCG CG[GA][CT]^CG
BstBI 1310 TTCGAA TT^CGAA
BstYI 920 GGATCT [GA]^GATC[CT]
CpoI 289 CGGACCG CG^G[AT]CCG
DraIII 1646 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 150 CGGCCG C^GGCCG
EarI 1809 GAAGAG CTCTTC(1/4)
EclXI 150 CGGCCG C^GGCCG
Eco52I 150 CGGCCG C^GGCCG
EcoNI 984 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1175 TGATCA T^GATCA
Hin1I 166 GGCGTC G[GA]^CG[CT]C
HindIII 1314 AAGCTT A^AGCTT
Ksp632I 1809 GAAGAG CTCTTC(1/4)
MflI 920 GGATCT [GA]^GATC[CT]
MseI 862 TTAA T^TAA
NheI 184 GCTAGC G^CTAGC
NspV 1310 TTCGAA TT^CGAA
PflFI 163 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 943 CACGTG CAC^GTG
PmlI 943 CACGTG CAC^GTG
RsrII 289 CGGACCG CG^G[AT]CCG
SapI 1809 GAAGAGC GCTCTTC(1/4)
ScaI 255 AGTACT AGT^ACT
SexAI 1106 ACCTGGT A^CC[AT]GGT
SfuI 1310 TTCGAA TT^CGAA
SmaI 544 CCCGGG CCC^GGG
SpeI 1423 ACTAGT A^CTAGT
StuI 1116 AGGCCT AGG^CCT
Tru9I 862 TTAA T^TAA
Tth111I 163 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 920 GGATCT [GA]^GATC[CT]
XmaCI 544 CCCGGG C^CCGGG
XmaI 544 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 287 TCCGGA T^CCGGA
2153 TCCGGA
AocI 405 CCTCAGG CC^T[ACGT]AGG
2065 CCTCAGG
BalI 785 TGGCCA TGG^CCA
1277 TGGCCA
BlpI 4 GCTGAGC GC^T[ACGT]AGC
836 GCTCAGC
BpmI 2417 CTCCAG CTGGAG(16/14)
901 CTGGAG
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
836 GCTCAGC
BsaWI 287 TCCGGA [AT]^CCGG[AT]
2153 TCCGGA
BseAI 287 TCCGGA T^CCGGA
2153 TCCGGA
BsmBI 727 GAGACG CGTCTC(1/5)
994 GAGACG
BspEI 287 TCCGGA T^CCGGA
2153 TCCGGA
BstEII 1167 GGTCACC G^GT[ACGT]ACC
1479 GGTGACC
BstPI 1167 GGTCACC G^GT[ACGT]ACC
1479 GGTGACC
Bsu36I 405 CCTCAGG CC^T[ACGT]AGG
2065 CCTCAGG
CelII 4 GCTGAGC GC^T[ACGT]AGC
836 GCTCAGC
Eco81I 405 CCTCAGG CC^T[ACGT]AGG
2065 CCTCAGG
EcoO65I 1167 GGTCACC G^GT[ACGT]ACC
1479 GGTGACC
HaeII 1782 GGCGCT [GA]GCGC^[CT]
2332 GGCGCT
HincII 1408 GTCAAC GT[CT]^[GA]AC
1659 GTTGAC
HindII 1408 GTCAAC GT[CT]^[GA]AC
1659 GTTGAC
KspI 207 CCGCGG CCGC^GG
551 CCGCGG
MaeII 944 ACGT A^CGT
1054 ACGT
MluNI 785 TGGCCA TGG^CCA
1277 TGGCCA
MroI 287 TCCGGA T^CCGGA
2153 TCCGGA
MscI 785 TGGCCA TGG^CCA
1277 TGGCCA
PpuMI 401 GGGTCCT [GA]G^G[AT]CC[CT]
1791 GGGACCC
SacII 207 CCGCGG CCGC^GG
551 CCGCGG
SspI 1611 AATATT AAT^ATT
1972 AATATT
TaqI 855 TCGA T^CGA
1311 TCGA
TthHB8I 855 TCGA T^CGA
1311 TCGA
XcmI 890 CCAGTGCAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2186 CCACCAGTGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI Asp718I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KpnI MaeI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I TspRI Van91I