Restriction map for
pFN21AB5751
(Linear) (Six-base) MAPSORT of: KIBB5751.seq Check: 7296 from: 1 to: 2424
KIBB5751.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 430 1233 1908 2424
Size: 430 803 675 516
Fragments arranged by size:
803 675 516 430
AccI GT'mk_AC
Cuts at: 0 2087 2424
Size: 2087 337
Acc65I G'GTAC_C
Cuts at: 0 103 1103 1223 2424
Size: 103 1000 120 1201
Fragments arranged by size:
1201 1000 120 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 2424
Size: 99 2325
AflIII A'CryG_T
Cuts at: 0 1052 2424
Size: 1052 1372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1940 2423 2424
Size: 1940 483 1
Fragments arranged by size:
1940 483 1
AlwNI CAG_nnn'CTG
Cuts at: 0 1233 1749 1822 1839 1866 2023 2232 2424
Size: 1233 516 73 17 27 157 209 192
Fragments arranged by size:
1233 516 209 192 157 73 27 17
ApaI G_GGCC'C
Cuts at: 0 2043 2424
Size: 2043 381
ApaLI G'TGCA_C
Cuts at: 0 946 2424
Size: 946 1478
AvaI C'yCGr_G
Cuts at: 0 544 649 1037 2141 2424
Size: 544 105 388 1104 283
Fragments arranged by size:
1104 544 388 283 105
BanI G'GyrC_C
Cuts at: 0 103 212 321 442 663 1103 1223 2424
Size: 103 109 109 121 221 440 120 1201
Fragments arranged by size:
1201 440 221 121 120 109 109 103
BanII G_rGCy'C
Cuts at: 0 57 543 724 1600 2043 2424
Size: 57 486 181 876 443 381
Fragments arranged by size:
876 486 443 381 181 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 1954 2424
Size: 1954 470
BbvCI CC'TCA_GC
Cuts at: 0 2287 2424
Size: 2287 137
BclI T'GATC_A
Cuts at: 0 1175 2424
Size: 1175 1249
BglI GCCn_nnn'nGGC
Cuts at: 0 1285 2424
Size: 1285 1139
BlpI GC'TnA_GC
Cuts at: 0 5 837 2424
Size: 5 832 1587
Fragments arranged by size:
1587 832 5
Bme1580I G_kGCm'C
Cuts at: 0 326 447 668 950 2043 2196 2424
Size: 326 121 221 282 1093 153 228
Fragments arranged by size:
1093 326 282 228 221 153 121
BmgBI CAC'GTC
Cuts at: 0 1055 2424
Size: 1055 1369
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 496 585 1643 2397 2424
Size: 285 211 89 1058 754 27
Fragments arranged by size:
1058 754 285 211 89 27
BmtI G_CTAG'C
Cuts at: 0 188 2424
Size: 188 2236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 253 546 1088 2271 2424
Size: 253 293 542 1183 153
Fragments arranged by size:
1183 542 293 253 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 2402 2424
Size: 922 1480 22
Fragments arranged by size:
1480 922 22
Bpu10I CC'TnA_GC
Cuts at: 0 364 2246 2287 2424
Size: 364 1882 41 137
Fragments arranged by size:
1882 364 137 41
BsaAI yAC'GTr
Cuts at: 0 945 2424
Size: 945 1479
BsaHI Gr'CG_yC
Cuts at: 0 167 2424
Size: 167 2257
BsaWI w'CCGG_w
Cuts at: 0 287 2153 2424
Size: 287 1866 271
Fragments arranged by size:
1866 287 271
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 985 1015 2424
Size: 985 30 1409
Fragments arranged by size:
1409 985 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 759 1342 1590 2424
Size: 759 583 248 834
Fragments arranged by size:
834 759 583 248
BseYI C'CCAG_C
Cuts at: 0 75 246 371 387 2095 2279 2424
Size: 75 171 125 16 1708 184 145
Fragments arranged by size:
1708 184 171 145 125 75 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 914 1261 1894 2419 2424
Size: 556 358 347 633 525 5
Fragments arranged by size:
633 556 525 358 347 5
BsiEI CG_ry'CG
Cuts at: 0 153 2424
Size: 153 2271
BsiHKAI G_wGCw'C
Cuts at: 0 383 950 1816 1901 2424
Size: 383 567 866 85 523
Fragments arranged by size:
866 567 523 383 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 721 988 2424
Size: 721 267 1436
Fragments arranged by size:
1436 721 267
Bsp1286I G_dGCh'C
Cuts at: 0 57 326 383 447 543 668 724 950
Size: 57 269 57 64 96 125 56 226
Cuts at: 950 1600 1816 1901 2043 2196 2424
Size: 650 216 85 142 153 228
Fragments arranged by size:
650 269 228 226 216 153 142 125
96 85 64 57 57 56
BspEI T'CCGG_A
Cuts at: 0 287 2153 2424
Size: 287 1866 271
Fragments arranged by size:
1866 287 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 430 1233 1908 1916 2424
Size: 430 803 675 8 508
Fragments arranged by size:
803 675 508 430 8
BsrBI CCG'CTC
Cuts at: 0 202 2250 2261 2424
Size: 202 2048 11 163
Fragments arranged by size:
2048 202 163 11
BstBI TT'CG_AA
Cuts at: 0 1311 2424
Size: 1311 1113
BstEII G'GTnAC_C
Cuts at: 0 1167 1479 2424
Size: 1167 312 945
Fragments arranged by size:
1167 945 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1212 1275 2196 2424
Size: 1212 63 921 228
Fragments arranged by size:
1212 921 228 63
BstYI r'GATC_y
Cuts at: 0 920 2424
Size: 920 1504
Bsu36I CC'TnA_GG
Cuts at: 0 406 2066 2424
Size: 406 1660 358
Fragments arranged by size:
1660 406 358
BtgI C'CryG_G
Cuts at: 0 207 551 935 1163 2393 2424
Size: 207 344 384 228 1230 31
Fragments arranged by size:
1230 384 344 228 207 31
BtsI GCAGTG_nn'
Cuts at: 0 337 619 902 1506 1934 2135 2424
Size: 337 282 283 604 428 201 289
Fragments arranged by size:
604 428 337 289 283 282 201
DraIII CAC_nnn'GTG
Cuts at: 0 1651 2424
Size: 1651 773
EaeI y'GGCC_r
Cuts at: 0 150 785 1277 1295 2424
Size: 150 635 492 18 1129
Fragments arranged by size:
1129 635 492 150 18
EagI C'GGCC_G
Cuts at: 0 150 2424
Size: 150 2274
EarI CTCTTCn'nnn_
Cuts at: 0 1804 2424
Size: 1804 620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 922 2402 2424
Size: 99 823 1480 22
Fragments arranged by size:
1480 823 99 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 988 2424
Size: 988 1436
EcoO109I rG'GnC_Cy
Cuts at: 0 119 392 402 1680 1792 2039 2424
Size: 119 273 10 1278 112 247 385
Fragments arranged by size:
1278 385 273 247 119 112 10
HaeII r_GCGC'y
Cuts at: 0 1786 2336 2424
Size: 1786 550 88
Fragments arranged by size:
1786 550 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 512 544 1039 1068 1071 1100 1276 1308
Size: 512 32 495 29 3 29 176 32
Cuts at: 1308 2135 2167 2321 2353 2424
Size: 827 32 154 32 71
Fragments arranged by size:
827 512 495 176 154 71 32 32
32 32 29 29 3
HincII GTy'rAC
Cuts at: 0 1410 1661 2424
Size: 1410 251 763
Fragments arranged by size:
1410 763 251
HindIII A'AGCT_T
Cuts at: 0 1314 2424
Size: 1314 1110
KpnI G_GTAC'C
Cuts at: 0 107 1107 1227 2424
Size: 107 1000 120 1197
Fragments arranged by size:
1197 1000 120 107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1032 1850 2424
Size: 1032 818 574
Fragments arranged by size:
1032 818 574
MscI TGG'CCA
Cuts at: 0 787 1279 2424
Size: 787 492 1145
Fragments arranged by size:
1145 787 492
MspA1I CmG'CkG
Cuts at: 0 209 218 371 553 616 1476 1863 2020
Size: 209 9 153 182 63 860 387 157
Cuts at: 2020 2106 2424
Size: 86 318
Fragments arranged by size:
860 387 318 209 182 157 153 86
63 9
NcoI C'CATG_G
Cuts at: 0 935 1163 2393 2424
Size: 935 228 1230 31
Fragments arranged by size:
1230 935 228 31
NheI G'CTAG_C
Cuts at: 0 184 2424
Size: 184 2240
PasI CC'CwG_GG
Cuts at: 0 447 2424
Size: 447 1977
PflMI CCAn_nnn'nTGG
Cuts at: 0 1233 1551 1651 2424
Size: 1233 318 100 773
Fragments arranged by size:
1233 773 318 100
PfoI T'CCnGG_A
Cuts at: 0 1414 2174 2339 2424
Size: 1414 760 165 85
Fragments arranged by size:
1414 760 165 85
PmlI CAC'GTG
Cuts at: 0 945 2424
Size: 945 1479
PpuMI rG'GwC_Cy
Cuts at: 0 402 1792 2424
Size: 402 1390 632
Fragments arranged by size:
1390 632 402
PspOMI G'GGCC_C
Cuts at: 0 2039 2424
Size: 2039 385
PstI C_TGCA'G
Cuts at: 0 1835 1929 2028 2412 2424
Size: 1835 94 99 384 12
Fragments arranged by size:
1835 384 99 94 12
PvuII CAG'CTG
Cuts at: 0 1476 1863 2020 2106 2424
Size: 1476 387 157 86 318
Fragments arranged by size:
1476 387 318 157 86
RsrII CG'GwC_CG
Cuts at: 0 290 2424
Size: 290 2134
SacII CC_GC'GG
Cuts at: 0 210 554 2424
Size: 210 344 1870
Fragments arranged by size:
1870 344 210
SanDI GG'GwC_CC
Cuts at: 0 1792 2424
Size: 1792 632
SapI GCTCTTCn'nnn_
Cuts at: 0 1804 2424
Size: 1804 620
ScaI AGT'ACT
Cuts at: 0 257 2424
Size: 257 2167
SexAI A'CCwGG_T
Cuts at: 0 1106 2424
Size: 1106 1318
SfcI C'TryA_G
Cuts at: 0 1304 1741 1831 1925 2024 2408 2424
Size: 1304 437 90 94 99 384 16
Fragments arranged by size:
1304 437 384 99 94 90 16
SmaI CCC'GGG
Cuts at: 0 546 2424
Size: 546 1878
SpeI A'CTAG_T
Cuts at: 0 1423 2424
Size: 1423 1001
SspI AAT'ATT
Cuts at: 0 1613 1974 2424
Size: 1613 361 450
Fragments arranged by size:
1613 450 361
StuI AGG'CCT
Cuts at: 0 1118 2424
Size: 1118 1306
StyI C'CwwG_G
Cuts at: 0 935 1163 2393 2424
Size: 935 228 1230 31
Fragments arranged by size:
1230 935 228 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 267 651 901 1558 1662 2293 2424
Size: 267 384 250 657 104 631 131
Fragments arranged by size:
657 631 384 267 250 131 104
TatI w'GTAC_w
Cuts at: 0 255 1581 2166 2424
Size: 255 1326 585 258
Fragments arranged by size:
1326 585 258 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 680 712 2148 2180 2424
Size: 680 32 1436 32 244
Fragments arranged by size:
1436 680 244 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 166 2424
Size: 166 2258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 897 2193 2424
Size: 897 1296 231
Fragments arranged by size:
1296 897 231
XmaI C'CCGG_G
Cuts at: 0 544 2424
Size: 544 1880
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BbvCI BclI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
KpnI MmeI MscI MspA1I NcoI NheI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII RsrII SacII
SanDI SapI ScaI SexAI SfcI SmaI SpeI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BfrBI BglII BpuEI BsaI BsaBI BsiWI
BsmI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI
MfeI MluI MslI NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI SacI SalI SbfI SfiI
SfoI SgfI SgrAI SmlI SnaBI SphI SrfI SwaI
XbaI XhoI XmnI ZraI