Restriction map for
pFN21AB7984
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbsI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraIII Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I MaeI
MamI MboI MfeI MflI MluI
MroI MseI MunI NaeI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 780 GAATTC [GA]^AATT[CT]
AfaI 531 GTAC GT^AC
Alw44I 166 GTGCAC G^TGCAC
AlwNI 313 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 166 GTGCAC G^TGCAC
ApoI 780 GAATTC [GA]^AATT[CT]
Asp700I 480 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 863 TGGCCA TGG^CCA
BbeI 244 GGCGCC GGCGC^C
BbrPI 846 CACGTG CAC^GTG
BbvCI 704 CCTCAGC CCTCAGC(-5/-2)
BsaAI 846 CACGTG [CT]AC^GT[GA]
BsgI 916 GTGCAG GTGCAG(16/14)
BsmBI 63 CGTCTC CGTCTC(1/5)
BsrFI 962 ACCGGC [GA]^CCGG[CT]
Cfr10I 962 ACCGGC [GA]^CCGG[CT]
DrdI 48 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 59 CGGCCG C^GGCCG
EclXI 59 CGGCCG C^GGCCG
Eco52I 59 CGGCCG C^GGCCG
EcoRI 780 GAATTC G^AATTC
HaeII 244 GGCGCC [GA]GCGC^[CT]
HincII 625 GTCAAC GT[CT]^[GA]AC
HindII 625 GTCAAC GT[CT]^[GA]AC
HphI 81 TCACC GGTGA(8/7)
KasI 244 GGCGCC G^GCGCC
KspI 785 CCGCGG CCGC^GG
MluNI 863 TGGCCA TGG^CCA
MscI 863 TGGCCA TGG^CCA
MslI 678 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 244 GGCGCC GG^CGCC
NcoI 101 CCATGG C^CATGG
PaeR7I 855 CTCGAG C^TCGAG
PflMI 312 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 846 CACGTG CAC^GTG
PmlI 846 CACGTG CAC^GTG
PpuMI 251 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 714 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 531 GTAC GT^AC
SacII 785 CCGCGG CCGC^GG
SfoI 244 GGCGCC GGC^GCC
SmaI 723 CCCGGG CCC^GGG
SmlI 855 CTCGAG C^T[CT][GA]AG
TaqI 856 TCGA T^CGA
TfiI 423 GAATC G^A[AT]TC
Tsp509I 781 AATT ^AATT
TthHB8I 856 TCGA T^CGA
Van91I 312 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 149 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 855 CTCGAG C^TCGAG
XmaCI 723 CCCGGG C^CCGGG
XmaI 723 CCCGGG C^CCGGG
XmnI 480 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 31 GAGCAC G[AT]GC[AT]^C
166 GTGCAC
BanII 505 GGGCTC G[GA]GC[CT]^C
726 GGGCTC
BsiEI 59 CGGCCG CG[GA][CT]^CG
133 CGGTCG
BsiHKAI 31 GAGCAC G[AT]GC[AT]^C
166 GTGCAC
BspLU11I 353 ACATGT A^CATGT
527 ACATGT
BstF5I 85 CATCC GGATG(2/0)
575 CATCC
DraII 251 AGGACCC [GA]G^G[ACGT]CC[CT]
566 AGGGCCT
DsaI 101 CCATGG C^C[GA][CT]GG
785 CCGCGG
EaeI 59 CGGCCG [CT]^GGCC[GA]
863 TGGCCA
EcoO109I 251 AGGACCC [GA]G^G[ACGT]CC[CT]
566 AGGGCCT
EcoT14I 101 CCATGG C^C[AT][AT]GG
563 CCAAGG
FauI 18 CCCGC CCCGC(4/6)
43 GCGGG
FokI 85 CATCC GGATG(9/13)
575 CATCC
HgaI 795 GACGC GACGC(5/10)
719 GCGTC
StyI 101 CCATGG C^C[AT][AT]GG
563 CCAAGG
Tsp45I 80 GTCAC ^GT[GC]AC
712 GTGAC
TspRI 710 CAGTG CA[GC]TG[ACGT][ACGT]^
956 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV NspI PleI Sau96I ScrFI
TseI