(Circular) (Six-base) MAPSORT of: pFN21AE1417.seq Check: 2615 from: 1 to: 6463 pFN21AE1417 6463 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3806 1937 Size: 1869 4594 Acc65I G'GTAC_C Cuts at: 2439 3784 2439 Size: 1345 5118 AclI AA'CG_TT Cuts at: 1563 4590 4963 1563 Size: 3027 373 3063 Fragments arranged by size: 3063 3027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 2283 2600 4526 5910 6178 1632 Size: 569 82 317 1926 1384 268 1917 Fragments arranged by size: 1926 1917 1384 569 317 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6266 AflIII A'CryG_T Cuts at: 1203 5362 6339 1203 Size: 4159 977 1327 Fragments arranged by size: 4159 1327 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 5192 5192 Size: 6463 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3273 1184 Size: 2089 4374 AlwNI CAG_nnn'CTG Cuts at: 2596 3598 4206 5778 2596 Size: 1002 608 1572 3281 Fragments arranged by size: 3281 1572 1002 608 ApaI G_GGCC'C Cuts at: 3121 3121 Size: 6463 ApaLI G'TGCA_C Cuts at: 2426 4520 5676 2426 Size: 2094 1156 3213 Fragments arranged by size: 3213 2094 1156 ApoI r'AATT_y Cuts at: 1493 3772 3998 4150 6259 1493 Size: 2279 226 152 2109 1697 Fragments arranged by size: 2279 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5017 160 Size: 872 3985 1606 Fragments arranged by size: 3985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 2931 3627 3788 1870 Size: 73 988 696 161 4545 Fragments arranged by size: 4545 988 696 161 73 BamHI G'GATC_C Cuts at: 2517 2841 3793 4160 2517 Size: 324 952 367 4820 Fragments arranged by size: 4820 952 367 324 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2439 2794 3784 5239 618 Size: 325 217 627 652 355 990 1455 1842 Fragments arranged by size: 1842 1455 990 652 627 355 325 217 BanII G_rGCy'C Cuts at: 1391 2283 3099 3121 3492 3782 1391 Size: 892 816 22 371 290 4072 Fragments arranged by size: 4072 892 816 371 290 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3080 3383 928 Size: 971 1181 303 4008 Fragments arranged by size: 4008 1181 971 303 BbvCI CC'TCA_GC Cuts at: 2274 2444 2274 Size: 170 6293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4654 4688 1903 Size: 34 50 34 2633 34 3678 Fragments arranged by size: 3678 2633 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4355 5565 4355 Size: 1210 5253 BclI T'GATC_A Cuts at: 1548 2095 1548 Size: 547 5916 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5074 136 Size: 107 122 71 631 605 3402 1525 Fragments arranged by size: 3402 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2346 6458 2346 Size: 4112 2351 BlpI GC'TnA_GC Cuts at: 3605 3880 3605 Size: 275 6188 Bme1580I G_kGCm'C Cuts at: 1790 2430 3121 3151 4524 5680 1790 Size: 640 691 30 1373 1156 2573 Fragments arranged by size: 2573 1373 1156 691 640 30 BmgBI CAC'GTC Cuts at: 1318 2822 1318 Size: 1504 4959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4194 5152 442 Size: 416 53 83 399 24 2777 958 1753 Fragments arranged by size: 2777 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2216 2978 3149 3233 5123 1239 Size: 833 144 762 171 84 1890 2579 Fragments arranged by size: 2579 1890 833 762 171 144 84 Bpu10I CC'TnA_GC Cuts at: 2274 2336 2444 2589 3201 2274 Size: 62 108 145 612 5536 Fragments arranged by size: 5536 612 145 108 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4588 5453 5751 4588 Size: 865 298 5300 Fragments arranged by size: 5300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5126 882 Size: 617 3627 2219 Fragments arranged by size: 3627 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4254 493 Size: 711 3050 2702 Fragments arranged by size: 3050 2702 711 BsaBI GATnn'nnATC Cuts at: 6457 6457 Size: 6463 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4270 4652 5287 275 Size: 53 83 186 936 2737 382 635 1451 Fragments arranged by size: 2737 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2514 4896 5294 5568 5715 6126 2514 Size: 2382 398 274 147 411 2851 Fragments arranged by size: 2851 2382 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6238 Fragments arranged by size: 6238 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2951 3053 3083 3146 3149 3170 3221 Size: 1105 660 102 30 63 3 21 51 Cuts at: 3221 3401 3560 1186 Size: 180 159 4089 Fragments arranged by size: 4089 1105 660 180 159 102 63 51 30 21 3 BseYI C'CCAG_C Cuts at: 5666 6162 5666 Size: 496 5967 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 2930 3174 3377 3605 1163 Size: 573 678 516 244 203 228 4021 Fragments arranged by size: 4021 678 573 516 244 228 203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 2376 2430 3099 4524 4609 5680 2283 Size: 93 54 669 1425 85 1071 3066 Fragments arranged by size: 3066 1425 1071 669 93 85 54 BsmI GAATG_Cn' Cuts at: 3974 4067 3974 Size: 93 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2376 2430 3099 3121 3151 3492 Size: 399 493 93 54 669 22 30 341 Cuts at: 3492 3782 4524 4609 5680 1391 Size: 290 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 493 399 341 290 93 85 54 30 22 BspEI T'CCGG_A Cuts at: 6126 6126 Size: 6463 BspHI T'CATG_A Cuts at: 4352 6082 4352 Size: 1730 4733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3818 844 Size: 2974 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2455 2962 4350 1394 Size: 429 632 507 1388 3507 Fragments arranged by size: 3507 1388 632 507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3547 4958 5132 65 Size: 1183 2299 1411 174 1396 Fragments arranged by size: 2299 1411 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3442 5107 5294 1700 Size: 110 1632 1665 187 2869 Fragments arranged by size: 2869 1665 1632 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 4523 5535 4523 Size: 1012 5451 BstAPI GCAn_nnn'nTGC Cuts at: 794 3025 794 Size: 2231 4232 BstBI TT'CG_AA Cuts at: 5267 5267 Size: 6463 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstYI r'GATC_y Cuts at: 1972 2346 2517 2841 3714 3793 4160 4545 4562 Size: 374 171 324 873 79 367 385 17 Cuts at: 4562 6014 6100 6112 6458 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 873 385 374 367 346 324 171 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2542 3000 3492 513 Size: 552 972 505 458 492 3484 Fragments arranged by size: 3484 972 552 505 492 458 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2355 487 Size: 683 56 1129 4595 Fragments arranged by size: 4595 1129 683 56 BtsI GCAGTG_nn' Cuts at: 3191 3276 3979 4773 4793 3191 Size: 85 703 794 20 4861 Fragments arranged by size: 4861 794 703 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6428 DraI TTT'AAA Cuts at: 3767 3767 Size: 6463 DraIII CAC_nnn'GTG Cuts at: 2152 3400 2152 Size: 1248 5215 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5470 809 Size: 507 4154 1802 Fragments arranged by size: 4154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3910 4799 6428 Size: 54 1425 50 275 18 2080 889 1629 Cuts at: 6428 8 Size: 43 Fragments arranged by size: 2080 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3294 3327 4393 1353 Size: 1941 33 1066 3423 Fragments arranged by size: 3423 1941 1066 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5050 5424 5570 387 Size: 1064 138 3461 374 146 1280 Fragments arranged by size: 3461 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2281 3097 2281 Size: 816 5647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2201 2216 2283 2600 2978 3149 Size: 393 440 129 15 67 317 378 171 Cuts at: 3149 3233 4526 5123 5910 6178 1239 Size: 84 1293 597 787 268 1524 Fragments arranged by size: 1524 1293 787 597 440 393 378 317 268 171 129 84 67 15 EcoNI CCTnn'n_nnAGG Cuts at: 3299 3299 Size: 6463 EcoO109I rG'GnC_Cy Cuts at: 2382 2593 3117 3118 3645 3757 2382 Size: 211 524 1 527 112 5088 Fragments arranged by size: 5088 527 524 211 112 1 EcoRI G'AATT_C Cuts at: 3772 4150 3772 Size: 378 6085 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2351 2737 2351 Size: 386 6077 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 3169 5610 1678 Size: 1491 2441 2531 Fragments arranged by size: 2531 2441 1491 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5104 5136 5178 5210 1373 Size: 32 135 32 3532 32 42 32 2626 Fragments arranged by size: 3532 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2706 3807 4053 669 Size: 988 281 768 1101 246 3079 Fragments arranged by size: 3079 1101 988 768 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6463 HpaI GTT'AAC Cuts at: 2706 4053 2706 Size: 1347 5116 KpnI G_GTAC'C Cuts at: 2443 3788 2443 Size: 1345 5118 MfeI C'AATT_G Cuts at: 4062 4062 Size: 6463 MluI A'CGCG_T Cuts at: 6339 6339 Size: 6463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2550 5577 5761 1305 Size: 1245 3027 184 2007 Fragments arranged by size: 3027 2007 1245 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4641 Fragments arranged by size: 4641 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2906 4421 4780 4939 518 Size: 648 1740 1515 359 159 2042 Fragments arranged by size: 2042 1740 1515 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2956 2971 3127 3253 3589 3595 3879 4556 Size: 1350 15 156 126 336 6 284 677 Cuts at: 4556 5704 5949 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1350 1148 677 336 284 245 156 126 15 6 NcoI C'CATG_G Cuts at: 513 1065 2037 513 Size: 552 972 4939 Fragments arranged by size: 4939 972 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6463 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 2534 3821 5366 1132 Size: 97 1305 1287 1545 2229 Fragments arranged by size: 2229 1545 1305 1287 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6463 PciI A'CATG_T Cuts at: 5362 5362 Size: 6463 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 2717 1108 Size: 1044 565 4854 Fragments arranged by size: 4854 1044 565 PfoI T'CCnGG_A Cuts at: 2121 2286 3294 3459 3528 2121 Size: 165 1008 165 69 5056 Fragments arranged by size: 5056 1008 165 165 69 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4532 4564 6045 6077 4532 Size: 32 1481 32 4918 Fragments arranged by size: 4918 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2382 2593 3645 2382 Size: 211 1052 5200 Fragments arranged by size: 5200 1052 211 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 4033 4033 Size: 6463 PspOMI G'GGCC_C Cuts at: 3117 3117 Size: 6463 PstI C_TGCA'G Cuts at: 830 2850 3060 3126 3815 830 Size: 2020 210 66 689 3478 Fragments arranged by size: 3478 2020 689 210 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 PvuII CAG'CTG Cuts at: 2956 2971 3127 3253 3595 2956 Size: 15 156 126 342 5824 Fragments arranged by size: 5824 342 156 126 15 SacI G_AGCT'C Cuts at: 2283 3099 2283 Size: 816 5647 SalI G'TCGA_C Cuts at: 1936 3805 1936 Size: 1869 4594 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 4711 6423 4711 Size: 1712 4751 SexAI A'CCwGG_T Cuts at: 3369 3396 3369 Size: 27 6436 SfcI C'TryA_G Cuts at: 826 1045 2846 3056 3122 3499 3811 4946 5627 Size: 219 1801 210 66 377 312 1135 681 Cuts at: 5627 5818 826 Size: 191 1471 Fragments arranged by size: 1801 1471 1135 681 377 312 219 210 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 2933 3790 1872 Size: 1061 857 4545 Fragments arranged by size: 4545 1061 857 SmlI C'TyrA_G Cuts at: 820 1017 1943 4567 5468 5730 820 Size: 197 926 2624 901 262 1553 Fragments arranged by size: 2624 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4254 493 Size: 3761 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6463 SphI G_CATG'C Cuts at: 1132 2534 3821 1132 Size: 1402 1287 3774 Fragments arranged by size: 3774 1402 1287 SspI AAT'ATT Cuts at: 5 52 2683 4387 5 Size: 47 2631 1704 2081 Fragments arranged by size: 2631 2081 1704 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6463 StyI C'CwwG_G Cuts at: 513 1065 2037 2671 2726 3381 3902 513 Size: 552 972 634 55 655 521 3074 Fragments arranged by size: 3074 972 655 634 552 521 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2312 4490 4507 4660 4845 5290 325 Size: 1255 732 2178 17 153 185 445 1498 Fragments arranged by size: 2178 1498 1255 732 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3696 4709 6421 Size: 274 80 33 51 1443 1719 1013 1712 Cuts at: 6421 96 Size: 138 Fragments arranged by size: 1719 1712 1443 1013 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6423 6455 6423 Size: 32 6431 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6463 XbaI T'CTAG_A Cuts at: 3799 3799 Size: 6463 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6463 XmaI C'CCGG_G Cuts at: 1870 2931 3788 1870 Size: 1061 857 4545 Fragments arranged by size: 4545 1061 857 XmnI GAAnn'nnTTC Cuts at: 2306 4592 2306 Size: 2286 4177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI