(Linear) (Six-base) MAPSORT of: KIEE1417.seq Check: 2427 from: 1 to: 1758 KIEE1417.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. Acc65I G'GTAC_C Cuts at: 0 434 1758 Size: 434 1324 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 278 595 1758 Size: 196 82 317 1163 Fragments arranged by size: 1163 317 196 82 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1268 1758 Size: 1268 490 AlwNI CAG_nnn'CTG Cuts at: 0 591 1593 1758 Size: 591 1002 165 Fragments arranged by size: 1002 591 165 ApaI G_GGCC'C Cuts at: 0 1116 1758 Size: 1116 642 ApaLI G'TGCA_C Cuts at: 0 421 1758 Size: 421 1337 AvaI C'yCGr_G Cuts at: 0 926 1622 1758 Size: 926 696 136 Fragments arranged by size: 926 696 136 BamHI G'GATC_C Cuts at: 0 512 836 1758 Size: 512 324 922 Fragments arranged by size: 922 512 324 BanI G'GyrC_C Cuts at: 0 434 789 1758 Size: 434 355 969 Fragments arranged by size: 969 434 355 BanII G_rGCy'C Cuts at: 0 278 1094 1116 1487 1758 Size: 278 816 22 371 271 Fragments arranged by size: 816 371 278 271 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 1075 1378 1758 Size: 1075 303 380 Fragments arranged by size: 1075 380 303 BbvCI CC'TCA_GC Cuts at: 0 269 439 1758 Size: 269 170 1319 Fragments arranged by size: 1319 269 170 BclI T'GATC_A Cuts at: 0 90 1758 Size: 90 1668 BglII A'GATC_T Cuts at: 0 341 1758 Size: 341 1417 BlpI GC'TnA_GC Cuts at: 0 1600 1758 Size: 1600 158 Bme1580I G_kGCm'C Cuts at: 0 425 1116 1146 1758 Size: 425 691 30 612 Fragments arranged by size: 691 612 425 30 BmgBI CAC'GTC Cuts at: 0 817 1758 Size: 817 941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 211 973 1144 1228 1758 Size: 67 144 762 171 84 530 Fragments arranged by size: 762 530 171 144 84 67 Bpu10I CC'TnA_GC Cuts at: 0 269 331 439 584 1196 1758 Size: 269 62 108 145 612 562 Fragments arranged by size: 612 562 269 145 108 62 BsaWI w'CCGG_w Cuts at: 0 509 1758 Size: 509 1249 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 286 946 1048 1078 1141 1144 1165 1216 Size: 286 660 102 30 63 3 21 51 Cuts at: 1216 1396 1555 1758 Size: 180 159 203 Fragments arranged by size: 660 286 203 180 159 102 63 51 30 21 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 925 1169 1372 1600 1758 Size: 409 516 244 203 228 158 Fragments arranged by size: 516 409 244 228 203 158 BsiHKAI G_wGCw'C Cuts at: 0 278 371 425 1094 1758 Size: 278 93 54 669 664 Fragments arranged by size: 669 664 278 93 54 Bsp1286I G_dGCh'C Cuts at: 0 278 371 425 1094 1116 1146 1487 1758 Size: 278 93 54 669 22 30 341 271 Fragments arranged by size: 669 341 278 271 93 54 30 22 BsrBI CCG'CTC Cuts at: 0 450 957 1758 Size: 450 507 801 Fragments arranged by size: 801 507 450 BsrDI GCAATG_nn' Cuts at: 0 1542 1758 Size: 1542 216 BsrFI r'CCGG_y Cuts at: 0 1437 1758 Size: 1437 321 BstAPI GCAn_nnn'nTGC Cuts at: 0 1020 1758 Size: 1020 738 BstYI r'GATC_y Cuts at: 0 341 512 836 1709 1758 Size: 341 171 324 873 49 Fragments arranged by size: 873 341 324 171 49 BtgI C'CryG_G Cuts at: 0 32 537 995 1487 1758 Size: 32 505 458 492 271 Fragments arranged by size: 505 492 458 271 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 350 1758 Size: 350 1408 BtsI GCAGTG_nn' Cuts at: 0 1186 1271 1758 Size: 1186 85 487 Fragments arranged by size: 1186 487 85 DraIII CAC_nnn'GTG Cuts at: 0 147 1395 1758 Size: 147 1248 363 Fragments arranged by size: 1248 363 147 EarI CTCTTCn'nnn_ Cuts at: 0 1289 1322 1758 Size: 1289 33 436 Fragments arranged by size: 1289 436 33 EcoICRI GAG'CTC Cuts at: 0 276 1092 1758 Size: 276 816 666 Fragments arranged by size: 816 666 276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 196 211 278 595 973 1144 1228 Size: 67 129 15 67 317 378 171 84 Cuts at: 1228 1758 Size: 530 Fragments arranged by size: 530 378 317 171 129 84 67 67 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 1294 1758 Size: 1294 464 EcoO109I rG'GnC_Cy Cuts at: 0 377 588 1112 1113 1640 1752 1758 Size: 377 211 524 1 527 112 6 Fragments arranged by size: 527 524 377 211 112 6 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 346 732 1758 Size: 346 386 1026 Fragments arranged by size: 1026 386 346 HaeII r_GCGC'y Cuts at: 0 1164 1758 Size: 1164 594 HincII GTy'rAC Cuts at: 0 701 1758 Size: 701 1057 HpaI GTT'AAC Cuts at: 0 701 1758 Size: 701 1057 KpnI G_GTAC'C Cuts at: 0 438 1758 Size: 438 1320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 545 1758 Size: 545 1213 MslI CAynn'nnrTG Cuts at: 0 901 1758 Size: 901 857 MspA1I CmG'CkG Cuts at: 0 951 966 1122 1248 1584 1590 1758 Size: 951 15 156 126 336 6 168 Fragments arranged by size: 951 336 168 156 126 15 6 NcoI C'CATG_G Cuts at: 0 32 1758 Size: 32 1726 NspI r_CATG'y Cuts at: 0 529 1758 Size: 529 1229 PflMI CCAn_nnn'nTGG Cuts at: 0 147 712 1758 Size: 147 565 1046 Fragments arranged by size: 1046 565 147 PfoI T'CCnGG_A Cuts at: 0 116 281 1289 1454 1523 1758 Size: 116 165 1008 165 69 235 Fragments arranged by size: 1008 235 165 165 116 69 PpuMI rG'GwC_Cy Cuts at: 0 377 588 1640 1758 Size: 377 211 1052 118 Fragments arranged by size: 1052 377 211 118 PspOMI G'GGCC_C Cuts at: 0 1112 1758 Size: 1112 646 PstI C_TGCA'G Cuts at: 0 845 1055 1121 1758 Size: 845 210 66 637 Fragments arranged by size: 845 637 210 66 PvuII CAG'CTG Cuts at: 0 951 966 1122 1248 1590 1758 Size: 951 15 156 126 342 168 Fragments arranged by size: 951 342 168 156 126 15 SacI G_AGCT'C Cuts at: 0 278 1094 1758 Size: 278 816 664 Fragments arranged by size: 816 664 278 SexAI A'CCwGG_T Cuts at: 0 1364 1391 1758 Size: 1364 27 367 Fragments arranged by size: 1364 367 27 SfcI C'TryA_G Cuts at: 0 841 1051 1117 1494 1758 Size: 841 210 66 377 264 Fragments arranged by size: 841 377 264 210 66 SmaI CCC'GGG Cuts at: 0 928 1758 Size: 928 830 SphI G_CATG'C Cuts at: 0 529 1758 Size: 529 1229 SspI AAT'ATT Cuts at: 0 678 1758 Size: 678 1080 StyI C'CwwG_G Cuts at: 0 32 666 721 1376 1758 Size: 32 634 55 655 382 Fragments arranged by size: 655 634 382 55 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 307 1758 Size: 307 1451 TatI w'GTAC_w Cuts at: 0 1691 1758 Size: 1691 67 XmaI C'CCGG_G Cuts at: 0 926 1758 Size: 926 832 XmnI GAAnn'nnTTC Cuts at: 0 301 1758 Size: 301 1457 Enzymes that do cut: Acc65I AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaWI BseRI BsgI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstYI BtgI BtgZI BtsI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII HincII HpaI KpnI MmeI MslI MspA1I NcoI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SexAI SfcI SmaI SphI SspI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BglI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EaeI EagI EciI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI