################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstNI BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeII MamI MfeI MluI MluNI MroI MscI MunI MvaI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AseI 754 ATTAAT AT^TAAT AsnI 754 ATTAAT AT^TAAT AvaI 478 CCCGGG C^[CT]CG[GA]G BbsI 1138 GAAGAC GAAGAC(2/6) BciVI 783 GGATAC GTATCC(6/5) BclI 980 TGATCA T^GATCA BfaI 313 CTAG C^TAG BpuAI 1138 GAAGAC GAAGAC(2/6) BsaI 301 GAGACC GGTCTC(1/5) BsaWI 956 TCCGGT [AT]^CCGG[AT] BseRI 1189 GAGGAG GAGGAG(10/8) BsoBI 478 CCCGGG C^[CT]CG[GA]G BstAPI 435 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 849 GCGC GCG^C DraI 117 TTTAAA TTT^AAA DraIII 788 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 877 ATGCAT ATGCA^T FauI 1004 GCGGG CCCGC(4/6) FbaI 980 TGATCA T^GATCA Fnu4HI 38 GCGGC GC^[ACGT]GC HhaI 849 GCGC GCG^C HinP1I 849 GCGC G^CGC ItaI 38 GCGGC GC^[ACGT]GC KspI 14 CCGCGG CCGC^GG MaeI 313 CTAG C^TAG MslI 1200 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 14 CCGCGG C[AC]G^C[GT]G NsiI 877 ATGCAT ATGCA^T NspI 879 GCATGC [GA]CATG^[CT] PleI 124 GAGTC GAGTC(4/5) PshBI 754 ATTAAT AT^TAAT PstI 442 CTGCAG CTGCA^G SacII 14 CCGCGG CCGC^GG SmaI 478 CCCGGG CCC^GGG SmlI 224 CTCAAG C^T[CT][GA]AG SpeI 312 ACTAGT A^CTAGT SphI 879 GCATGC GCATG^C XcmI 326 CCACCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 478 CCCGGG C^CCGGG XmaI 478 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 15 CGCG CG^CG 1003 CGCG AluI 186 AGCT AG^CT 228 AGCT AlwNI 236 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG 896 CAGTTTCTG AvaII 54 GGACC G^G[AT]CC 921 GGACC BcnI 478 CCCGG CC^[GC]GG 479 CCGGG BspHI 683 TCATGA T^CATGA 944 TCATGA BssKI 478 CCCGG ^CC[ACGT]GG 479 CCGGG BstBI 422 TTCGAA TT^CGAA 698 TTCGAA BstF5I 270 CATCC GGATG(2/0) 546 GGATG BstUI 15 CGCG CG^CG 1003 CGCG BstXI 329 CCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1199 CCATGAATGTGG BstYI 347 AGATCC [GA]^GATC[CT] 690 AGATCC DdeI 230 CTCAG C^T[ACGT]AG 869 CTGAG DsaI 14 CCGCGG C^C[GA][CT]GG 570 CCGTGG EcoRI 701 GAATTC G^AATTC 952 GAATTC FokI 270 CATCC GGATG(9/13) 546 GGATG HaeIII 40 GGCC GG^CC 321 GGCC HapII 479 CCGG C^CGG 957 CCGG HpaII 479 CCGG C^CGG 957 CCGG HphI 708 GGTGA GGTGA(8/7) 913 GGTGA MflI 347 AGATCC [GA]^GATC[CT] 690 AGATCC MspI 479 CCGG C^CGG 957 CCGG MvnI 15 CGCG CG^CG 1003 CGCG MwoI 253 GCCACATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 435 GCAAAAACTGC NciI 478 CCCGG CC^[GC]GG 479 CCGGG NspV 422 TTCGAA TT^CGAA 698 TTCGAA RcaI 683 TCATGA T^CATGA 944 TCATGA ScrFI 478 CCCGG CC^[ACGT]GG 479 CCGGG SfcI 442 CTGCAG C^T[GA][CT]AG 1181 CTACAG SfuI 422 TTCGAA TT^CGAA 698 TTCGAA TfiI 737 GAATC G^A[AT]TC 1149 GATTC TspRI 809 CACTG CA[GC]TG[ACGT][ACGT]^ 1184 CAGTG XhoII 347 AGATCC [GA]^GATC[CT] 690 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AcsI AfaI AlwI ApoI BsaJI BsmAI BsrI Cac8I Cfr13I DpnII HinfI MaeIII MboI MboII MnlI MseI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I SfaNI TaqI Tru9I Tsp45I Tsp509I TthHB8I