(Circular) (Six-base) MAPSORT of: pFN21AB4740.seq Check: 9862 from: 1 to: 7315 pFN21AB4740 7315 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2782 4336 4523 2782 Size: 1554 187 5574 Fragments arranged by size: 5574 1554 187 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2768 5125 278 Size: 53 83 186 936 1232 2357 2468 Fragments arranged by size: 2468 2357 1232 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4658 1937 Size: 2721 4594 Acc65I G'GTAC_C Cuts at: 4403 4636 4403 Size: 233 7082 AclI AA'CG_TT Cuts at: 1563 5442 5815 1563 Size: 3879 373 3063 Fragments arranged by size: 3879 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2586 2862 3145 4085 5378 6762 7030 1632 Size: 954 276 283 940 1293 1384 268 1917 Fragments arranged by size: 1917 1384 1293 954 940 283 276 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7118 AflIII A'CryG_T Cuts at: 1203 2947 6214 7191 1203 Size: 1744 3267 977 1327 Fragments arranged by size: 3267 1744 1327 977 AgeI A'CCGG_T Cuts at: 4400 6146 4400 Size: 1746 5569 AhdI GACnn_n'nnGTC Cuts at: 3937 6044 3937 Size: 2107 5208 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7315 AlwNI CAG_nnn'CTG Cuts at: 2732 2888 5058 6630 2732 Size: 156 2170 1572 3417 Fragments arranged by size: 3417 2170 1572 156 ApaI G_GGCC'C Cuts at: 3221 3221 Size: 7315 ApaLI G'TGCA_C Cuts at: 5372 6528 5372 Size: 1156 6159 ApoI r'AATT_y Cuts at: 1493 4624 4850 5002 7111 1493 Size: 3131 226 152 2109 1697 Fragments arranged by size: 3131 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5869 160 Size: 872 4837 1606 Fragments arranged by size: 4837 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 AvaI C'yCGr_G Cuts at: 1870 1943 3030 3433 4146 4640 1870 Size: 73 1087 403 713 494 4545 Fragments arranged by size: 4545 1087 713 494 403 73 BamHI G'GATC_C Cuts at: 3304 3424 4377 4645 5012 3304 Size: 120 953 268 367 5607 Fragments arranged by size: 5607 953 367 268 120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2294 2538 2655 4403 4636 Size: 325 217 627 507 244 117 1748 233 Cuts at: 4636 6091 618 Size: 1455 1842 Fragments arranged by size: 1842 1748 1455 627 507 325 244 233 217 117 BanII G_rGCy'C Cuts at: 1391 2192 3221 4634 1391 Size: 801 1029 1413 4072 Fragments arranged by size: 4072 1413 1029 801 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2174 3177 928 Size: 971 275 1003 5066 Fragments arranged by size: 5066 1003 971 275 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 5506 5540 1903 Size: 34 1883 34 1652 34 3678 Fragments arranged by size: 3678 1883 1652 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5207 6417 5207 Size: 1210 6105 BclI T'GATC_A Cuts at: 1548 1548 Size: 7315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2688 2837 5926 Size: 107 122 71 631 605 1016 149 3089 Cuts at: 5926 136 Size: 1525 Fragments arranged by size: 3089 1525 1016 631 605 149 122 107 71 BglII A'GATC_T Cuts at: 7310 7310 Size: 7315 BlpI GC'TnA_GC Cuts at: 4732 4732 Size: 7315 Bme1580I G_kGCm'C Cuts at: 1790 2473 3221 5376 6532 1790 Size: 683 748 2155 1156 2573 Fragments arranged by size: 2573 2155 1156 748 683 BmgBI CAC'GTC Cuts at: 1318 2948 1318 Size: 1630 5685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2148 2181 3289 Size: 416 53 83 399 24 731 33 1108 Cuts at: 3289 4049 5046 6004 442 Size: 760 997 958 1753 Fragments arranged by size: 1753 1108 997 958 760 731 416 399 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2281 2491 2511 2988 3125 3956 4028 4226 Size: 1042 210 20 477 137 831 72 198 Cuts at: 4226 4259 5975 1239 Size: 33 1716 2579 Fragments arranged by size: 2579 1716 1042 831 477 210 198 137 72 33 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2296 2806 5440 6305 6603 2296 Size: 510 2634 865 298 3008 Fragments arranged by size: 3008 2634 865 510 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2153 3910 4586 5978 882 Size: 617 654 1757 676 1392 2219 Fragments arranged by size: 2219 1757 1392 676 654 617 BsaAI yAC'GTr Cuts at: 493 1204 2128 3100 3271 3547 5106 493 Size: 711 924 972 171 276 1559 2702 Fragments arranged by size: 2702 1559 972 924 711 276 171 BsaBI GATnn'nnATC Cuts at: 7309 7309 Size: 7315 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2765 5122 5504 6139 Size: 53 83 186 936 1232 2357 382 635 Cuts at: 6139 275 Size: 1451 Fragments arranged by size: 2357 1451 1232 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4400 5748 6146 6420 6567 6978 4400 Size: 1348 398 274 147 411 4737 Fragments arranged by size: 4737 1348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2862 2892 3770 3800 1178 Size: 30 165 30 1459 30 878 30 4693 Fragments arranged by size: 4693 1459 878 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3671 3778 4112 4604 1186 Size: 2485 107 334 492 3897 Fragments arranged by size: 3897 2485 492 334 107 BseYI C'CCAG_C Cuts at: 2549 2617 2827 2855 2881 3009 3234 6518 7014 Size: 68 210 28 26 128 225 3284 496 Cuts at: 7014 2549 Size: 2850 Fragments arranged by size: 3284 2850 496 225 210 128 68 28 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2763 1163 Size: 573 1027 5715 Fragments arranged by size: 5715 1027 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3090 4765 5526 6554 1540 Size: 275 186 1089 1675 761 1028 2301 Fragments arranged by size: 2301 1675 1089 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2192 2647 3720 4295 5376 5461 6532 2192 Size: 455 1073 575 1081 85 1071 2975 Fragments arranged by size: 2975 1081 1073 1071 575 455 85 BsmI GAATG_Cn' Cuts at: 4826 4919 4826 Size: 93 7222 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2473 2647 3221 3720 4295 4634 Size: 399 402 281 174 574 499 575 339 Cuts at: 4634 5376 5461 6532 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 574 499 402 399 339 281 174 85 BspEI T'CCGG_A Cuts at: 6978 6978 Size: 7315 BspHI T'CATG_A Cuts at: 3600 5204 6934 3600 Size: 1604 1730 3981 Fragments arranged by size: 3981 1730 1604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 2782 3027 3630 4336 4415 4523 4670 Size: 1535 403 245 603 706 79 108 147 Cuts at: 4670 844 Size: 3489 Fragments arranged by size: 3489 1535 706 603 403 245 147 108 79 BsrBI CCG'CTC Cuts at: 1394 1823 2992 5202 1394 Size: 429 1169 2210 3507 Fragments arranged by size: 3507 2210 1169 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5810 5984 65 Size: 1183 4562 174 1396 Fragments arranged by size: 4562 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2535 2624 2652 2836 3630 4302 4400 Size: 110 725 89 28 184 794 672 98 Cuts at: 4400 5959 6146 1700 Size: 1559 187 2869 Fragments arranged by size: 2869 1559 794 725 672 187 184 110 98 89 28 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7315 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7315 BssSI C'ACGA_G Cuts at: 3710 4058 5375 6387 3710 Size: 348 1317 1012 4638 Fragments arranged by size: 4638 1317 1012 348 BstAPI GCAn_nnn'nTGC Cuts at: 794 2559 794 Size: 1765 5550 BstBI TT'CG_AA Cuts at: 3579 6119 3579 Size: 2540 4775 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7315 BstYI r'GATC_y Cuts at: 1972 2573 3025 3304 3424 4329 4377 4531 4645 Size: 601 452 279 120 905 48 154 114 Cuts at: 4645 5012 5397 5414 6866 6952 6964 7310 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 905 601 452 385 367 346 279 154 120 114 86 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3090 3843 3856 513 Size: 552 939 1086 753 13 3972 Fragments arranged by size: 3972 1086 939 753 552 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3664 3823 487 Size: 683 56 2438 159 3979 Fragments arranged by size: 3979 2438 683 159 56 BtsI GCAGTG_nn' Cuts at: 2473 3122 4831 5625 5645 2473 Size: 649 1709 794 20 4143 Fragments arranged by size: 4143 1709 794 649 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7315 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7280 DraI TTT'AAA Cuts at: 2253 4619 2253 Size: 2366 4949 DraIII CAC_nnn'GTG Cuts at: 3715 3715 Size: 7315 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6322 809 Size: 507 5006 1802 Fragments arranged by size: 5006 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2694 4762 5651 Size: 54 1425 50 275 18 864 2068 889 Cuts at: 5651 7280 8 Size: 1629 43 Fragments arranged by size: 2068 1629 1425 889 864 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4762 1537 Size: 275 2950 4090 Fragments arranged by size: 4090 2950 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4104 4152 4283 4367 5245 1353 Size: 2751 48 131 84 878 3423 Fragments arranged by size: 3423 2751 878 131 84 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2421 5902 6276 6422 387 Size: 1064 138 832 3481 374 146 1280 Fragments arranged by size: 3481 1280 1064 832 374 146 138 EcoICRI GAG'CTC Cuts at: 2190 2190 Size: 7315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2281 2491 2511 2586 2862 2988 3125 Size: 393 649 210 20 75 276 126 137 Cuts at: 3125 3145 3956 4028 4085 4226 4259 5378 5975 Size: 20 811 72 57 141 33 1119 597 Cuts at: 5975 6762 7030 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1119 811 787 649 597 393 276 268 210 141 137 126 75 72 57 33 20 20 EcoO109I rG'GnC_Cy Cuts at: 2896 3218 3661 2896 Size: 322 443 6550 Fragments arranged by size: 6550 443 322 EcoRI G'AATT_C Cuts at: 4624 5002 4624 Size: 378 6937 FspI TGC'GCA Cuts at: 3104 5821 3104 Size: 2717 4598 HaeII r_GCGC'y Cuts at: 1678 3851 6462 1678 Size: 2173 2611 2531 Fragments arranged by size: 2611 2531 2173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2785 2817 3923 3955 5956 Size: 32 135 32 1213 32 1106 32 2001 Cuts at: 5956 5988 6030 6062 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2001 1213 1106 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2108 2308 2962 4659 4905 669 Size: 988 281 170 200 654 1697 246 3079 Fragments arranged by size: 3079 1697 988 654 281 246 200 170 HindIII A'AGCT_T Cuts at: 748 748 Size: 7315 HpaI GTT'AAC Cuts at: 4905 4905 Size: 7315 KpnI G_GTAC'C Cuts at: 4407 4640 4407 Size: 233 7082 MfeI C'AATT_G Cuts at: 4914 4914 Size: 7315 MluI A'CGCG_T Cuts at: 7191 7191 Size: 7315 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 3110 3616 6429 6613 1305 Size: 1035 770 506 2813 184 2007 Fragments arranged by size: 2813 2007 1035 770 506 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2696 10 Size: 54 1425 343 864 4629 Fragments arranged by size: 4629 1425 864 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2595 3528 3605 3644 3842 5273 5632 Size: 648 1429 933 77 39 198 1431 359 Cuts at: 5632 5791 518 Size: 159 2042 Fragments arranged by size: 2042 1431 1429 933 648 359 198 159 77 39 MspA1I CmG'CkG Cuts at: 1606 2359 2491 2885 3092 3130 4204 4561 4731 Size: 753 132 394 207 38 1074 357 170 Cuts at: 4731 5408 6556 6801 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1074 753 677 394 357 245 207 170 132 38 NaeI GCC'GGC Cuts at: 2654 3632 2654 Size: 978 6337 NcoI C'CATG_G Cuts at: 513 1065 2004 3843 3856 513 Size: 552 939 1839 13 3972 Fragments arranged by size: 3972 1839 939 552 13 NdeI CA'TA_TG Cuts at: 387 387 Size: 7315 NgoMIV G'CCGG_C Cuts at: 2652 3630 2652 Size: 978 6337 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7315 NotI GC'GGCC_GC Cuts at: 4762 4762 Size: 7315 NspI r_CATG'y Cuts at: 1132 1229 2356 4261 4673 6218 1132 Size: 97 1127 1905 412 1545 2229 Fragments arranged by size: 2229 1905 1545 1127 412 97 PasI CC'CwG_GG Cuts at: 1348 3214 3316 1348 Size: 1866 102 5347 Fragments arranged by size: 5347 1866 102 PciI A'CATG_T Cuts at: 6214 6214 Size: 7315 PflMI CCAn_nnn'nTGG Cuts at: 1108 2985 3075 3690 3972 4030 1108 Size: 1877 90 615 282 58 4393 Fragments arranged by size: 4393 1877 615 282 90 58 PfoI T'CCnGG_A Cuts at: 2424 2576 3327 4023 4176 2424 Size: 152 751 696 153 5563 Fragments arranged by size: 5563 751 696 153 152 PmeI GTTT'AAAC Cuts at: 4619 4619 Size: 7315 PmlI CAC'GTG Cuts at: 3547 3547 Size: 7315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5384 5416 6897 6929 5384 Size: 32 1481 32 5770 Fragments arranged by size: 5770 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7315 PsiI TTA'TAA Cuts at: 4885 4885 Size: 7315 PspOMI G'GGCC_C Cuts at: 3217 3217 Size: 7315 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3491 3523 3491 Size: 32 7283 PstI C_TGCA'G Cuts at: 830 2376 3774 4392 4667 830 Size: 1546 1398 618 275 3478 Fragments arranged by size: 3478 1546 1398 618 275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7315 PvuII CAG'CTG Cuts at: 2359 2491 4561 2359 Size: 132 2070 5113 Fragments arranged by size: 5113 2070 132 SacI G_AGCT'C Cuts at: 2192 2192 Size: 7315 SacII CC_GC'GG Cuts at: 3093 3093 Size: 7315 SalI G'TCGA_C Cuts at: 1936 4657 1936 Size: 2721 4594 SapI GCTCTTCn'nnn_ Cuts at: 4152 4283 4152 Size: 131 7184 SbfI CC_TGCA'GG Cuts at: 4667 4667 Size: 7315 ScaI AGT'ACT Cuts at: 5563 7275 5563 Size: 1712 5603 SfcI C'TryA_G Cuts at: 826 1045 2372 3770 4388 4663 5798 6479 6670 Size: 219 1327 1398 618 275 1135 681 191 Cuts at: 6670 826 Size: 1471 Fragments arranged by size: 1471 1398 1327 1135 681 618 275 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7315 SmaI CCC'GGG Cuts at: 1872 3435 4642 1872 Size: 1563 1207 4545 Fragments arranged by size: 4545 1563 1207 SmlI C'TyrA_G Cuts at: 820 1017 1943 2275 2785 5419 6320 6582 820 Size: 197 926 332 510 2634 901 262 1553 Fragments arranged by size: 2634 1553 926 901 510 332 262 197 SnaBI TAC'GTA Cuts at: 493 5106 493 Size: 4613 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7315 SphI G_CATG'C Cuts at: 1132 4261 4673 1132 Size: 3129 412 3774 Fragments arranged by size: 3774 3129 412 SspI AAT'ATT Cuts at: 5 52 5239 5 Size: 47 5187 2081 Fragments arranged by size: 5187 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7315 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 3843 3856 4754 513 Size: 552 939 87 1752 13 898 3074 Fragments arranged by size: 3074 1752 939 898 552 87 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4610 5342 5359 5512 5697 6142 325 Size: 1255 3030 732 17 153 185 445 1498 Fragments arranged by size: 3030 1498 1255 732 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4014 4113 5561 Size: 274 80 33 51 1443 2037 99 1448 Cuts at: 5561 7273 96 Size: 1712 138 Fragments arranged by size: 2037 1712 1448 1443 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2862 2894 7275 7307 2862 Size: 32 4381 32 2870 Fragments arranged by size: 4381 2870 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7315 XbaI T'CTAG_A Cuts at: 4651 4651 Size: 7315 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4174 4174 Size: 7315 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7315 XmaI C'CCGG_G Cuts at: 1870 3433 4640 1870 Size: 1563 1207 4545 Fragments arranged by size: 4545 1563 1207 XmnI GAAnn'nnTTC Cuts at: 5444 5444 Size: 7315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2766 5123 276 Size: 53 83 186 936 1232 2357 2468 Fragments arranged by size: 2468 2357 1232 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspXI RsrII SanDI SexAI SfiI SfoI SrfI SwaI