##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoRV       EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HincII      HindII      HinfI       HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AspHI         223  GAGCTC               G[AT]GC[AT]^C
BanII         223  GAGCTC               G[GA]GC[CT]^C
BmyI          223  GAGCTC               G[AGT]GC[ACT]^C
BsiHKAI       223  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      223  GAGCTC               G[AGT]GC[ACT]^C
BsrI          248  ACTGG                ACTGG(1/-1)
Cac8I          12  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          182  GCGC                 GCG^C
DsaI           17  CCGTGG               C^C[GA][CT]GG
EaeI           14  TGGCCG               [CT]^GGCC[GA]
EcoRI         291  GAATTC               G^AATTC
HaeIII         15  GGCC                 GG^CC
HhaI          182  GCGC                 GCG^C
HinP1I        182  GCGC                 G^CGC
HindIII       205  AAGCTT               A^AGCTT
MwoI           22  GCTCTCTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         234  GGACCC               GG[ACGT]^[ACGT]CC
PstI           47  CTGCAG               CTGCA^G
SacI          223  GAGCTC               GAGCT^C
SfcI           47  CTGCAG               C^T[GA][CT]AG
XcmI            8  CCAAGCTGGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          139  AAATTT               [GA]^AATT[CT]
              291  GAATTC              
ApoI          139  AAATTT               [GA]^AATT[CT]
              291  GAATTC              
AvaII         172  GGACC                G^G[AT]CC
              234  GGACC               
BbvI           31  GCAGC                GCAGC(8/12)
               49  GCAGC               
BsgI           65  GTGCAG               GTGCAG(16/14)
              253  GTGCAG              
Cfr13I        172  GGACC                G^G[ACGT]CC
              234  GGACC               
EarI          218  GAAGAG               CTCTTC(1/4)
              279  GAAGAG              
EcoT14I        76  CCAAGG               C^C[AT][AT]GG
              239  CCAAGG              
Fnu4HI         31  GCAGC                GC^[ACGT]GC
               49  GCAGC               
ItaI           31  GCAGC                GC^[ACGT]GC
               49  GCAGC               
Ksp632I       218  GAAGAG               CTCTTC(1/4)
              279  GAAGAG              
MboII         218  GAAGA                GAAGA(8/7)
              279  GAAGA               
MnlI          258  GAGG                 CCTC(7/6)
              282  GAGG                
Sau96I        172  GGACC                G^G[ACGT]CC
              234  GGACC               
StyI           76  CCAAGG               C^C[AT][AT]GG
              239  CCAAGG              
TseI           31  GCAGC                G^C[AT]GC
               49  GCAGC               
TspRI         103  CAGTG                CA[GC]TG[ACGT][ACGT]^
              178  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AluI        BsaJI       DdeI        Tsp509I