################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AspI BamHI BciVI BclI BfrI BglII BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BssSI Bst1107I BstEII BstPI BstZ17I CelII Cfr10I CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmeI PshAI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 934 GACGTC GACGT^C AccI 952 GTCTAC GT^[AC][GT]AC AcsI 241 GAATTC [GA]^AATT[CT] AflIII 911 ACATGT A^C[GA][CT]GT AhdI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1311 GGGCCC GGGCC^C ApoI 241 GAATTC [GA]^AATT[CT] AseI 222 ATTAAT AT^TAAT AsnI 222 ATTAAT AT^TAAT Asp700I 2035 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2090 TGCGCA TGC^GCA AvrII 846 CCTAGG C^CTAGG BbeI 18 GGCGCC GGCGC^C BbrPI 1792 CACGTG CAC^GTG BcgI 1191 GCAGGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 846 CCTAGG C^CTAGG BsgI 1002 CTGCAC GTGCAG(16/14) BsiEI 156 CGATCG CG[GA][CT]^CG BsmBI 510 GAGACG CGTCTC(1/5) BsmFI 1545 GGGAC GGGAC(10/14) Bsp1407I 261 TGTACA T^GTACA BspDI 383 ATCGAT AT^CGAT BspLU11I 911 ACATGT A^CATGT BspMI 1779 GCAGGT ACCTGC(4/8) BsrGI 261 TGTACA T^GTACA BssHII 1911 GCGCGC G^CGCGC BstAPI 1106 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 359 TTCGAA TT^CGAA BstXI 419 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 383 ATCGAT AT^CGAT DrdI 1714 GACAATCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 241 GAATTC G^AATTC FspI 2090 TGCGCA TGC^GCA HaeII 18 GGCGCC [GA]GCGC^[CT] KasI 18 GGCGCC G^GCGCC MfeI 1110 CAATTG C^AATTG MseI 223 TTAA T^TAA MunI 1110 CAATTG C^AATTG NarI 18 GGCGCC GG^CGCC NspV 359 TTCGAA TT^CGAA PaeR7I 803 CTCGAG C^TCGAG PmaCI 1792 CACGTG CAC^GTG PmlI 1792 CACGTG CAC^GTG PpuMI 194 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 222 ATTAAT AT^TAAT PvuI 156 CGATCG CGAT^CG SacI 376 GAGCTC GAGCT^C SfoI 18 GGCGCC GGC^GCC SfuI 359 TTCGAA TT^CGAA SspBI 261 TGTACA T^GTACA StuI 1877 AGGCCT AGG^CCT Tru9I 223 TTAA T^TAA XcmI 1991 CCAAGGTACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 803 CTCGAG C^TCGAG XmnI 2035 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 63 CGCG CG^CG 1912 CGCG AcyI 18 GGCGCC G[GA]^CG[CT]C 934 GACGTC AvaI 191 CTCGGG C^[CT]CG[GA]G 803 CTCGAG BalI 209 TGGCCA TGG^CCA 1988 TGGCCA BbsI 592 GAAGAC GAAGAC(2/6) 648 GAAGAC BbvCI 1249 GCTGAGG CCTCAGC(-5/-2) 2029 GCTGAGG BpuAI 592 GAAGAC GAAGAC(2/6) 648 GAAGAC BsaAI 868 CACGTA [CT]AC^GT[GA] 1792 CACGTG BsaHI 18 GGCGCC G[GA]^CG[CT]C 934 GACGTC BsaI 658 GGTCTC GGTCTC(1/5) 823 GGTCTC BsoBI 191 CTCGGG C^[CT]CG[GA]G 803 CTCGAG BstUI 63 CGCG CG^CG 1912 CGCG DraII 194 GGGTCCT [GA]G^G[ACGT]CC[CT] 1032 AGGGCCT DsaI 1053 CCATGG C^C[GA][CT]GG 1985 CCATGG EcoO109I 194 GGGTCCT [GA]G^G[ACGT]CC[CT] 1032 AGGGCCT Hin1I 18 GGCGCC G[GA]^CG[CT]C 934 GACGTC MluNI 209 TGGCCA TGG^CCA 1988 TGGCCA MscI 209 TGGCCA TGG^CCA 1988 TGGCCA MvnI 63 CGCG CG^CG 1912 CGCG NcoI 1053 CCATGG C^CATGG 1985 CCATGG NspI 863 ACATGC [GA]CATG^[CT] 911 ACATGT ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI Eco81I EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII