(Circular) (Six-base) MAPSORT of: pFN21AE4568.seq Check: 4361 from: 1 to: 6868 pFN21AE4568 6868 bp With 182 enzymes: SgfI * March 28, 2012 14:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2943 4678 278 Size: 53 83 186 936 1407 1735 2468 Fragments arranged by size: 2468 1735 1407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2958 4211 1937 Size: 1021 1253 4594 Fragments arranged by size: 4594 1253 1021 Acc65I G'GTAC_C Cuts at: 4189 4189 Size: 6868 AclI AA'CG_TT Cuts at: 1563 4995 5368 1563 Size: 3432 373 3063 Fragments arranged by size: 3432 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 4179 4931 6315 6583 1632 Size: 713 1834 752 1384 268 1917 Fragments arranged by size: 1917 1834 1384 752 713 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6671 AflIII A'CryG_T Cuts at: 1203 2916 5767 6744 1203 Size: 1713 2851 977 1327 Fragments arranged by size: 2851 1713 1327 977 AgeI A'CCGG_T Cuts at: 5699 5699 Size: 6868 AhdI GACnn_n'nnGTC Cuts at: 2661 5597 2661 Size: 2936 3932 AleI CACnn'nnGTG Cuts at: 1166 3159 1166 Size: 1993 4875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6868 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2706 2738 2706 Size: 32 6836 AlwNI CAG_nnn'CTG Cuts at: 2757 3238 3544 3790 4611 6183 2757 Size: 481 306 246 821 1572 3442 Fragments arranged by size: 3442 1572 821 481 306 246 ApaI G_GGCC'C Cuts at: 3320 3320 Size: 6868 ApaLI G'TGCA_C Cuts at: 4925 6081 4925 Size: 1156 5712 ApoI r'AATT_y Cuts at: 1493 2246 4177 4403 4555 6664 1493 Size: 753 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 753 226 152 AseI AT'TA_AT Cuts at: 160 1032 2228 5422 160 Size: 872 1196 3194 1606 Fragments arranged by size: 3194 1606 1196 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2808 4193 1870 Size: 73 253 612 1385 4545 Fragments arranged by size: 4545 1385 612 253 73 AvrII C'CTAG_G Cuts at: 2851 2851 Size: 6868 BamHI G'GATC_C Cuts at: 4198 4565 4198 Size: 367 6501 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2337 2894 4189 5644 Size: 325 217 627 236 314 557 1295 1455 Cuts at: 5644 618 Size: 1842 Fragments arranged by size: 1842 1455 1295 627 557 325 314 236 217 BanII G_rGCy'C Cuts at: 1391 2385 2497 3095 3320 3344 3893 4187 1391 Size: 994 112 598 225 24 549 294 4072 Fragments arranged by size: 4072 994 598 549 294 225 112 24 BbeI G_GCGC'C Cuts at: 2027 2027 Size: 6868 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2604 2660 928 Size: 971 705 56 5136 Fragments arranged by size: 5136 971 705 56 BbvCI CC'TCA_GC Cuts at: 3255 4035 3255 Size: 780 6088 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3185 3219 5059 5093 1903 Size: 34 1248 34 1840 34 3678 Fragments arranged by size: 3678 1840 1248 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4760 5970 4760 Size: 1210 5658 BclI T'GATC_A Cuts at: 1548 1548 Size: 6868 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2105 2222 Size: 107 122 71 631 605 337 96 117 Cuts at: 2222 3630 5479 136 Size: 1408 1849 1525 Fragments arranged by size: 1849 1525 1408 631 605 337 122 117 107 96 71 BglII A'GATC_T Cuts at: 6863 6863 Size: 6868 BlpI GC'TnA_GC Cuts at: 4285 4285 Size: 6868 Bme1580I G_kGCm'C Cuts at: 1790 2899 3320 3862 4929 6085 1790 Size: 1109 421 542 1067 1156 2573 Fragments arranged by size: 2573 1156 1109 1067 542 421 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6868 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2405 4599 5557 Size: 416 53 83 399 24 988 2194 958 Cuts at: 5557 442 Size: 1753 Fragments arranged by size: 2194 1753 988 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6868 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2423 3027 2423 Size: 604 6264 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3237 3348 3827 3907 4043 5528 1239 Size: 1998 111 479 80 136 1485 2579 Fragments arranged by size: 2579 1998 1485 479 136 111 80 Bpu10I CC'TnA_GC Cuts at: 2883 3255 4035 2883 Size: 372 780 5716 Fragments arranged by size: 5716 780 372 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2519 3539 3731 3829 4993 5858 6156 2519 Size: 1020 192 98 1164 865 298 3231 Fragments arranged by size: 3231 1164 1020 865 298 192 98 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2669 2834 5531 882 Size: 617 1170 165 2697 2219 Fragments arranged by size: 2697 2219 1170 617 165 BsaAI yAC'GTr Cuts at: 493 1204 2875 3799 4659 493 Size: 711 1671 924 860 2702 Fragments arranged by size: 2702 1671 924 860 711 BsaBI GATnn'nnATC Cuts at: 6862 6862 Size: 6868 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 2940 4675 5057 Size: 53 83 186 936 491 916 1735 382 Cuts at: 5057 5692 275 Size: 635 1451 Fragments arranged by size: 1735 1451 936 916 635 491 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5301 5699 5973 6120 6531 5301 Size: 398 274 147 411 5638 Fragments arranged by size: 5638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2706 2736 1178 Size: 30 165 30 1303 30 5310 Fragments arranged by size: 5310 1303 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2754 2858 3539 3626 3716 4163 1186 Size: 940 628 104 681 87 90 447 3891 Fragments arranged by size: 3891 940 681 628 447 104 90 87 BseYI C'CCAG_C Cuts at: 2573 6071 6567 2573 Size: 3498 496 2874 Fragments arranged by size: 3498 2874 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2992 1163 Size: 573 1256 5039 Fragments arranged by size: 5039 1256 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2164 4318 5079 6107 1540 Size: 275 186 163 2154 761 1028 2301 Fragments arranged by size: 2301 2154 1028 761 275 186 163 BsiHKAI G_wGCw'C Cuts at: 2385 3087 3223 4929 5014 6085 2385 Size: 702 136 1706 85 1071 3168 Fragments arranged by size: 3168 1706 1071 702 136 85 BsmI GAATG_Cn' Cuts at: 4379 4472 4379 Size: 93 6775 BsmBI CGTCTCn'nnnn_ Cuts at: 2509 2509 Size: 6868 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2497 2899 3087 3095 3223 3320 Size: 399 595 112 402 188 8 128 97 Cuts at: 3320 3344 3862 3893 4187 4929 5014 6085 1391 Size: 24 518 31 294 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 518 402 399 294 188 128 112 97 85 31 24 8 BspEI T'CCGG_A Cuts at: 6531 6531 Size: 6868 BspHI T'CATG_A Cuts at: 4757 6487 4757 Size: 1730 5138 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3775 4223 844 Size: 2931 448 3489 Fragments arranged by size: 3489 2931 448 BsrBI CCG'CTC Cuts at: 1394 1823 4755 1394 Size: 429 2932 3507 Fragments arranged by size: 3507 2932 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5363 5537 65 Size: 1183 4115 174 1396 Fragments arranged by size: 4115 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5512 5699 1700 Size: 110 3702 187 2869 Fragments arranged by size: 3702 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2266 96 Size: 2170 4698 BssHII G'CGCG_C Cuts at: 1925 3916 1925 Size: 1991 4877 BssSI C'ACGA_G Cuts at: 4928 5940 4928 Size: 1012 5856 BstAPI GCAn_nnn'nTGC Cuts at: 794 3117 794 Size: 2323 4545 BstBI TT'CG_AA Cuts at: 2365 5672 2365 Size: 3307 3561 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6868 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2431 Size: 6868 BstYI r'GATC_y Cuts at: 1972 2847 2862 3225 4026 4198 4565 4950 4967 Size: 875 15 363 801 172 367 385 17 Cuts at: 4967 6419 6505 6517 6863 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 875 801 385 367 363 346 172 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2766 3042 3336 2766 Size: 276 294 6298 Fragments arranged by size: 6298 294 276 BtgI C'CryG_G Cuts at: 513 1065 2004 3058 3990 513 Size: 552 939 1054 932 3391 Fragments arranged by size: 3391 1054 939 932 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6129 Fragments arranged by size: 6129 683 56 BtsI GCAGTG_nn' Cuts at: 4384 5178 5198 4384 Size: 794 20 6054 Fragments arranged by size: 6054 794 20 ClaI AT'CG_AT Cuts at: 1554 2389 1554 Size: 835 6033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6833 DraI TTT'AAA Cuts at: 4172 4172 Size: 6868 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3725 5875 809 Size: 507 2409 2150 1802 Fragments arranged by size: 2409 2150 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2214 3993 Size: 54 1425 50 275 18 318 66 1779 Cuts at: 3993 4315 5204 6833 8 Size: 322 889 1629 43 Fragments arranged by size: 1779 1629 1425 889 322 318 275 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4315 1537 Size: 275 2503 4090 Fragments arranged by size: 4090 2503 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3516 3567 4140 4798 1353 Size: 2163 51 573 658 3423 Fragments arranged by size: 3423 2163 658 573 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 2936 5455 5829 5975 387 Size: 1064 138 534 813 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 813 534 374 146 138 EcoICRI GAG'CTC Cuts at: 2383 2383 Size: 6868 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 3237 3348 3827 3907 4043 4179 Size: 393 713 892 111 479 80 136 136 Cuts at: 4179 4931 5528 6315 6583 1239 Size: 752 597 787 268 1524 Fragments arranged by size: 1524 892 787 752 713 597 479 393 268 136 136 111 80 EcoNI CCTnn'n_nnAGG Cuts at: 2696 3194 4034 2696 Size: 498 840 5530 Fragments arranged by size: 5530 840 498 EcoO109I rG'GnC_Cy Cuts at: 2200 3038 2200 Size: 838 6030 EcoRI G'AATT_C Cuts at: 2246 4177 4555 2246 Size: 1931 378 4559 Fragments arranged by size: 4559 1931 378 FspI TGC'GCA Cuts at: 4097 5374 4097 Size: 1277 5591 HaeII r_GCGC'y Cuts at: 1678 2027 6015 1678 Size: 349 3988 2531 Fragments arranged by size: 3988 2531 349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2456 2488 2647 2679 3210 Size: 32 135 32 884 32 159 32 531 Cuts at: 3210 3242 3543 3575 4139 4171 5509 5541 5583 Size: 32 301 32 564 32 1338 32 42 Cuts at: 5583 5615 1373 Size: 32 2626 Fragments arranged by size: 2626 1338 884 564 531 301 159 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4212 4458 669 Size: 988 281 2274 246 3079 Fragments arranged by size: 3079 2274 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6868 HpaI GTT'AAC Cuts at: 4458 4458 Size: 6868 KasI G'GCGC_C Cuts at: 2023 2023 Size: 6868 KpnI G_GTAC'C Cuts at: 4193 4193 Size: 6868 MfeI C'AATT_G Cuts at: 3115 4467 3115 Size: 1352 5516 MluI A'CGCG_T Cuts at: 6744 6744 Size: 6868 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2931 5982 6166 1305 Size: 1626 3051 184 2007 Fragments arranged by size: 3051 2007 1626 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 3995 10 Size: 54 1425 343 384 1779 2883 Fragments arranged by size: 2883 1779 1425 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3159 3512 3656 4826 5185 5344 518 Size: 648 1993 353 144 1170 359 159 2042 Fragments arranged by size: 2042 1993 1170 648 359 353 159 144 MspA1I CmG'CkG Cuts at: 1606 2064 2085 2927 3691 4021 4111 4284 4961 Size: 458 21 842 764 330 90 173 677 Cuts at: 4961 6109 6354 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 842 764 677 458 330 245 173 90 21 NarI GG'CG_CC Cuts at: 2024 2024 Size: 6868 NcoI C'CATG_G Cuts at: 513 1065 2004 3058 3990 513 Size: 552 939 1054 932 3391 Fragments arranged by size: 3391 1054 939 932 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6868 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6868 NotI GC'GGCC_GC Cuts at: 4315 4315 Size: 6868 NspI r_CATG'y Cuts at: 1132 1229 2872 2920 4226 5771 1132 Size: 97 1643 48 1306 1545 2229 Fragments arranged by size: 2229 1643 1545 1306 97 48 PasI CC'CwG_GG Cuts at: 1348 2659 1348 Size: 1311 5557 PciI A'CATG_T Cuts at: 2916 5767 2916 Size: 2851 4017 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6868 PfoI T'CCnGG_A Cuts at: 3729 3804 3729 Size: 75 6793 PmeI GTTT'AAAC Cuts at: 4172 4172 Size: 6868 PmlI CAC'GTG Cuts at: 3799 3799 Size: 6868 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2706 2738 4937 4969 6450 6482 2706 Size: 32 2199 32 1481 32 3092 Fragments arranged by size: 3092 2199 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2200 Size: 6868 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6868 PsiI TTA'TAA Cuts at: 4438 4438 Size: 6868 PspOMI G'GGCC_C Cuts at: 3316 3316 Size: 6868 PstI C_TGCA'G Cuts at: 830 3505 3537 4116 4220 830 Size: 2675 32 579 104 3478 Fragments arranged by size: 3478 2675 579 104 32 PvuI CG_AT'CG Cuts at: 2001 2164 2001 Size: 163 6705 PvuII CAG'CTG Cuts at: 2927 4021 4111 2927 Size: 1094 90 5684 Fragments arranged by size: 5684 1094 90 SacI G_AGCT'C Cuts at: 2385 2385 Size: 6868 SalI G'TCGA_C Cuts at: 1936 4210 1936 Size: 2274 4594 SbfI CC_TGCA'GG Cuts at: 4220 4220 Size: 6868 ScaI AGT'ACT Cuts at: 5116 6828 5116 Size: 1712 5156 SfcI C'TryA_G Cuts at: 826 1045 2016 3501 3533 3686 4112 4216 5351 Size: 219 971 1485 32 153 426 104 1135 Cuts at: 5351 6032 6223 826 Size: 681 191 1471 Fragments arranged by size: 1485 1471 1135 971 681 426 219 191 153 104 32 SfoI GGC'GCC Cuts at: 2025 2025 Size: 6868 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6868 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6868 SmaI CCC'GGG Cuts at: 1872 4195 1872 Size: 2323 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2498 2808 3554 3746 3844 4972 Size: 197 926 555 310 746 192 98 1128 Cuts at: 4972 5873 6135 820 Size: 901 262 1553 Fragments arranged by size: 1553 1128 926 901 746 555 310 262 197 192 98 SnaBI TAC'GTA Cuts at: 493 4659 493 Size: 4166 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6868 SphI G_CATG'C Cuts at: 1132 4226 1132 Size: 3094 3774 SspI AAT'ATT Cuts at: 5 52 4792 5 Size: 47 4740 2081 Fragments arranged by size: 4740 2081 47 StuI AGG'CCT Cuts at: 1517 3884 1517 Size: 2367 4501 StyI C'CwwG_G Cuts at: 513 1065 2004 2184 2424 2851 3058 3990 3996 Size: 552 939 180 240 427 207 932 6 Cuts at: 3996 4307 513 Size: 311 3074 Fragments arranged by size: 3074 939 932 552 427 311 240 207 180 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2595 3014 4895 4912 5065 5250 5695 Size: 1255 1015 419 1881 17 153 185 445 Cuts at: 5695 325 Size: 1498 Fragments arranged by size: 1881 1498 1255 1015 445 419 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2266 2919 5114 Size: 274 80 33 51 1443 289 653 2195 Cuts at: 5114 6826 96 Size: 1712 138 Fragments arranged by size: 2195 1712 1443 653 289 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3276 3308 6828 6860 3276 Size: 32 3520 32 3284 Fragments arranged by size: 3520 3284 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6868 XbaI T'CTAG_A Cuts at: 4204 4204 Size: 6868 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4003 4003 Size: 6868 XhoI C'TCGA_G Cuts at: 1943 2808 1943 Size: 865 6003 XmaI C'CCGG_G Cuts at: 1870 4193 1870 Size: 2323 4545 XmnI GAAnn'nnTTC Cuts at: 4044 4997 4044 Size: 953 5915 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2941 4676 276 Size: 53 83 186 936 1407 1735 2468 Fragments arranged by size: 2468 1735 1407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI