################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp718I AspI AvaII AvrII BamHI BbeI BcgI BciVI BfrI BlnI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoRI EcoRV HincII HindII HpaI KasI KpnI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI TfiI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 79 GACGCC G[GA]^CG[CT]C AhdI 465 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1583 AGCGCT AGC^GCT Asp700I 1470 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 465 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1451 TGCGCA TGC^GCA BbsI 1045 GTCTTC GAAGAC(2/6) BbvCI 900 CCTCAGC CCTCAGC(-5/-2) BglII 212 AGATCT A^GATCT BpuAI 1045 GTCTTC GAAGAC(2/6) BsaBI 1356 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 79 GACGCC G[GA]^CG[CT]C BsmI 874 GAATGC GAATGC(1/-1) BsrBI 1032 CCGCTC CCGCTC(-3/-3) BstAPI 1155 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 247 GGTCACC G^GT[ACGT]ACC BstPI 247 GGTCACC G^GT[ACGT]ACC BstXI 1485 CCACCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 465 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 448 GAAGAG CTCTTC(1/4) Eco47III 1583 AGCGCT AGC^GCT EcoNI 1935 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 247 GGTCACC G^GT[ACGT]ACC EcoT14I 640 CCAAGG C^C[AT][AT]GG EcoT22I 1220 ATGCAT ATGCA^T FseI 1639 GGCCGGCC GGCCGG^CC FspI 1451 TGCGCA TGC^GCA HgaI 79 GACGC GACGC(5/10) Hin1I 79 GACGCC G[GA]^CG[CT]C HindIII 1277 AAGCTT A^AGCTT Ksp632I 448 GAAGAG CTCTTC(1/4) KspI 1822 CCGCGG CCGC^GG MamI 1356 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 110 TTAA T^TAA NsiI 1220 ATGCAT ATGCA^T PaeR7I 1335 CTCGAG C^TCGAG SacI 43 GAGCTC GAGCT^C SacII 1822 CCGCGG CCGC^GG ScaI 71 AGTACT AGT^ACT SphI 1222 GCATGC GCATG^C SspI 908 AATATT AAT^ATT StuI 584 AGGCCT AGG^CCT StyI 640 CCAAGG C^C[AT][AT]GG Tru9I 110 TTAA T^TAA Tsp509I 1021 AATT ^AATT XhoI 1335 CTCGAG C^TCGAG XmnI 1470 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 816 CGCG CG^CG 1823 CGCG AfaI 72 GTAC GT^AC 857 GTAC AlwNI 1972 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 2036 CAGCTGCTG BalI 375 TGGCCA TGG^CCA 1619 TGGCCA BbrPI 1050 CACGTG CAC^GTG 1615 CACGTG BclI 1190 TGATCA T^GATCA 1847 TGATCA BfaI 475 CTAG C^TAG 826 CTAG BglI 636 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1340 GCCGGAATGGC BlpI 799 GCTGAGC GC^T[ACGT]AGC 832 GCTGAGC Bpu1102I 799 GCTGAGC GC^T[ACGT]AGC 832 GCTGAGC BsgI 794 GTGCAG GTGCAG(16/14) 1982 GTGCAG BsiEI 869 CGGTCG CG[GA][CT]^CG 1800 CGACCG BspLU11I 734 ACATGT A^CATGT 883 ACATGT BstUI 816 CGCG CG^CG 1823 CGCG BstYI 212 AGATCT [GA]^GATC[CT] 1793 AGATCC CelII 799 GCTGAGC GC^T[ACGT]AGC 832 GCTGAGC FbaI 1190 TGATCA T^GATCA 1847 TGATCA HaeII 1583 AGCGCT [GA]GCGC^[CT] 1878 GGCGCT HinfI 465 GACTC G^A[ACGT]TC 1930 GAGTC MaeI 475 CTAG C^TAG 826 CTAG MflI 212 AGATCT [GA]^GATC[CT] 1793 AGATCC MluNI 375 TGGCCA TGG^CCA 1619 TGGCCA MscI 375 TGGCCA TGG^CCA 1619 TGGCCA MvnI 816 CGCG CG^CG 1823 CGCG NaeI 432 GCCGGC GCC^GGC 1640 GCCGGC NgoMIV 432 GCCGGC G^CCGGC 1640 GCCGGC PleI 465 GACTC GAGTC(4/5) 1930 GAGTC PmaCI 1050 CACGTG CAC^GTG 1615 CACGTG PmlI 1050 CACGTG CAC^GTG 1615 CACGTG RsaI 72 GTAC GT^AC 857 GTAC SfaNI 1125 GATGC GCATC(5/9) 1356 GATGC SmlI 604 CTTGAG C^T[CT][GA]AG 1335 CTCGAG XcmI 83 CCACTCTGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 347 CCAAAACTTCAGTGG XhoII 212 AGATCT [GA]^GATC[CT] 1793 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AflIII AluI Alw44I AlwI AocI ApaI ApaLI AspHI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PstI PvuII Sau3AI Sau96I ScrFI SfcI TaqI TseI Tsp45I TspRI TthHB8I