Restriction map for pFN21AB6263
 (Linear) (Six-base) MAPSORT of: KIBB6263.seq  Check: 7381  from: 1  to: 2064



KIBB6263.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     361    1457    1784    2064 
   Size:        361    1096     327     280

  Fragments arranged by size: 

                1096     361     327     280
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     598     677    1545    2064 
   Size:        338     260      79     868     519

  Fragments arranged by size: 

                 868     519     338     260      79
 
AfeI AGC'GCT

Cuts at:      0    1585    2064 
   Size:       1585     479
 
AflIII A'CryG_T

Cuts at:      0     734     883    1392    2064 
   Size:        734     149     509     672

  Fragments arranged by size: 

                 734     672     509     149
 
AhdI GACnn_n'nnGTC

Cuts at:      0     470    2064 
   Size:        470    1594
 
AleI CACnn'nnGTG

Cuts at:      0     122    1292    1397    1463    1490    2064 
   Size:        122    1170     105      66      27     574

  Fragments arranged by size: 

                1170     574     122     105      66      27
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1907    2064 
   Size:       1907     157
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1977    2041    2064 
   Size:       1977      64      23

  Fragments arranged by size: 

                1977      64      23
 
ApaI G_GGCC'C

Cuts at:      0     395     556    1604    2064 
   Size:        395     161    1048     460

  Fragments arranged by size: 

                1048     460     395     161
 
ApaLI G'TGCA_C

Cuts at:      0    1099    1699    1737    2064 
   Size:       1099     600      38     327

  Fragments arranged by size: 

                1099     600     327      38
 
AvaI C'yCGr_G

Cuts at:      0     619    1335    2047    2064 
   Size:        619     716     712      17

  Fragments arranged by size: 

                 716     712     619      17
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     848     881    2064 
   Size:        848      33    1183

  Fragments arranged by size: 

                1183     848      33
 
BanI G'GyrC_C

Cuts at:      0     661     864    1325    2064 
   Size:        661     203     461     739

  Fragments arranged by size: 

                 739     661     461     203
 
BanII G_rGCy'C

Cuts at:      0      47     395     497     556     823    1209    1604    1912 
   Size:         47     348     102      59     267     386     395     308

Cuts at:   1912    2021    2064 
   Size:        109      43

  Fragments arranged by size: 

                 395     386     348     308     267     109     102      59
                  47      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1038    2064 
   Size:       1038    1026
 
BbvCI CC'TCA_GC

Cuts at:      0     901    2064 
   Size:        901    1163
 
BclI T'GATC_A

Cuts at:      0    1190    1847    2064 
   Size:       1190     657     217

  Fragments arranged by size: 

                1190     657     217
 
BfrBI ATG'CAT

Cuts at:      0    1222    2064 
   Size:       1222     842
 
BglI GCCn_nnn'nGGC

Cuts at:      0     642    1346    2064 
   Size:        642     704     718

  Fragments arranged by size: 

                 718     704     642
 
BglII A'GATC_T

Cuts at:      0     212    2064 
   Size:        212    1852
 
BlpI GC'TnA_GC

Cuts at:      0     800     833    2064 
   Size:        800      33    1231

  Fragments arranged by size: 

                1231     800      33
 
Bme1580I G_kGCm'C

Cuts at:      0     395     556     638     666    1103    1328    1604    1703 
   Size:        395     161      82      28     437     225     276      99

Cuts at:   1703    1741    2064 
   Size:         38     323

  Fragments arranged by size: 

                 437     395     323     276     225     161      99      82
                  38      28
 
BmgBI CAC'GTC

Cuts at:      0      93     423    1704    2064 
   Size:         93     330    1281     360

  Fragments arranged by size: 

                1281     360     330      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     950    2064 
   Size:        950    1114
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     573    1926    2064 
   Size:        573    1353     138

  Fragments arranged by size: 

                1353     573     138
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141    1024    1921    2035    2064 
   Size:        141     883     897     114      29

  Fragments arranged by size: 

                 897     883     141     114      29
 
Bpu10I CC'TnA_GC

Cuts at:      0     174     811     901    2064 
   Size:        174     637      90    1163

  Fragments arranged by size: 

                1163     637     174      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625    2064 
   Size:        625    1439
 
BsaAI yAC'GTr

Cuts at:      0    1052    1395    1617    2064 
   Size:       1052     343     222     447

  Fragments arranged by size: 

                1052     447     343     222
 
BsaBI GATnn'nnATC

Cuts at:      0    1360    2064 
   Size:       1360     704
 
BsaHI Gr'CG_yC

Cuts at:      0      80    2064 
   Size:         80    1984
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     549    1330    2002    2064 
   Size:        549     781     672      62

  Fragments arranged by size: 

                 781     672     549      62
 
BseYI C'CCAG_C

Cuts at:      0     387     524     537     665    1151    1958    2064 
   Size:        387     137      13     128     486     807     106

  Fragments arranged by size: 

                 807     486     387     137     128     106      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    2003    2064 
   Size:        815    1188      61

  Fragments arranged by size: 

                1188     815      61
 
BsiEI CG_ry'CG

Cuts at:      0     872    1803    2064 
   Size:        872     931     261

  Fragments arranged by size: 

                 931     872     261
 
BsiHKAI G_wGCw'C

Cuts at:      0      47     287    1057    1103    1703    1741    2064 
   Size:         47     240     770      46     600      38     323

  Fragments arranged by size: 

                 770     600     323     240      47      46      38
 
BsmI GAATG_Cn'

Cuts at:      0     880    2064 
   Size:        880    1184
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     287     395     497     556     638     666     823 
   Size:         47     240     108     102      59      82      28     157

Cuts at:    823    1057    1103    1209    1328    1604    1703    1741    1912 
   Size:        234      46     106     119     276      99      38     171

Cuts at:   1912    2021    2064 
   Size:        109      43

  Fragments arranged by size: 

                 276     240     234     171     157     119     109     108
                 106     102      99      82      59      47      46      43
                  38      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     361    1457    1784    2064 
   Size:        361    1096     327     280

  Fragments arranged by size: 

                1096     361     327     280
 
BsrBI CCG'CTC

Cuts at:      0    1034    2064 
   Size:       1034    1030
 
BsrFI r'CCGG_y

Cuts at:      0     432     867    1640    1999    2064 
   Size:        432     435     773     359      65

  Fragments arranged by size: 

                 773     435     432     359      65
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1161    2064 
   Size:       1161     903
 
BstEII G'GTnAC_C

Cuts at:      0     247    2064 
   Size:        247    1817
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1492    2064 
   Size:       1492     572
 
BstYI r'GATC_y

Cuts at:      0     212    1793    2064 
   Size:        212    1581     271

  Fragments arranged by size: 

                1581     271     212
 
Bsu36I CC'TnA_GG

Cuts at:      0     264     746    1609    2064 
   Size:        264     482     863     455

  Fragments arranged by size: 

                 863     482     455     264
 
BtgI C'CryG_G

Cuts at:      0    1290    1682    1822    2064 
   Size:       1290     392     140     242

  Fragments arranged by size: 

                1290     392     242     140
 
BtsI GCAGTG_nn'

Cuts at:      0     189    2064 
   Size:        189    1875
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1272    1307    2064 
   Size:       1272      35     757

  Fragments arranged by size: 

                1272     757      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      27    1107    1745    2064 
   Size:         27    1080     638     319

  Fragments arranged by size: 

                1080     638     319      27
 
EaeI y'GGCC_r

Cuts at:      0     375    1619    1968    2064 
   Size:        375    1244     349      96

  Fragments arranged by size: 

                1244     375     349      96
 
EarI CTCTTCn'nnn_

Cuts at:      0     443    2064 
   Size:        443    1621
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1066    1930    2064 
   Size:       1066     864     134

  Fragments arranged by size: 

                1066     864     134
 
EcoICRI GAG'CTC

Cuts at:      0      45    2064 
   Size:         45    2019
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     338     598     677    1024    1545    1921    2035 
   Size:        141     197     260      79     347     521     376     114

Cuts at:   2035    2064 
   Size:         29

  Fragments arranged by size: 

                 521     376     347     260     197     141     114      79
                  29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1939    2064 
   Size:       1939     125
 
EcoO109I rG'GnC_Cy

Cuts at:      0      99     391     392     521     552     553     644    1556 
   Size:         99     292       1     129      31       1      91     912

Cuts at:   1556    1600    1601    2064 
   Size:         44       1     463

  Fragments arranged by size: 

                 912     463     292     129      99      91      44      31
                   1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     603     999    1191    2064 
   Size:        603     396     192     873

  Fragments arranged by size: 

                 873     603     396     192
 
FseI GG_CCGG'CC

Cuts at:      0    1644    2064 
   Size:       1644     420
 
FspI TGC'GCA

Cuts at:      0    1453    2064 
   Size:       1453     611
 
HaeII r_GCGC'y

Cuts at:      0    1587    1882    2064 
   Size:       1587     295     182

  Fragments arranged by size: 

                1587     295     182
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     456     488     721     753    2064 
   Size:        456      32     233      32    1311

  Fragments arranged by size: 

                1311     456     233      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1277    2064 
   Size:       1277     787
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    2064 
   Size:        209    1855
 
MscI TGG'CCA

Cuts at:      0     377    1621    2064 
   Size:        377    1244     443

  Fragments arranged by size: 

                1244     443     377
 
MslI CAynn'nnrTG

Cuts at:      0     122    1292    1379    1395    1397    1463    1490    2064 
   Size:        122    1170      87      16       2      66      27     574

  Fragments arranged by size: 

                1170     574     122      87      66      27      16       2
            
 
MspA1I CmG'CkG

Cuts at:      0      57     669     799     832     862    1269    1824    2038 
   Size:         57     612     130      33      30     407     555     214

Cuts at:   2038    2064 
   Size:         26

  Fragments arranged by size: 

                 612     555     407     214     130      57      33      30
                  26
 
NaeI GCC'GGC

Cuts at:      0     434    1642    2064 
   Size:        434    1208     422

  Fragments arranged by size: 

                1208     434     422
 
NgoMIV G'CCGG_C

Cuts at:      0     432    1640    2064 
   Size:        432    1208     424

  Fragments arranged by size: 

                1208     432     424
 
NsiI A_TGCA'T

Cuts at:      0    1224    2064 
   Size:       1224     840
 
NspI r_CATG'y

Cuts at:      0     738     887    1226    1446    2064 
   Size:        738     149     339     220     618

  Fragments arranged by size: 

                 738     618     339     220     149
 
PasI CC'CwG_GG

Cuts at:      0     482    1941    2064 
   Size:        482    1459     123

  Fragments arranged by size: 

                1459     482     123
 
PciI A'CATG_T

Cuts at:      0     734     883    2064 
   Size:        734     149    1181

  Fragments arranged by size: 

                1181     734     149
 
PfoI T'CCnGG_A

Cuts at:      0    1001    2012    2064 
   Size:       1001    1011      52

  Fragments arranged by size: 

                1011    1001      52
 
PmlI CAC'GTG

Cuts at:      0    1052    1617    2064 
   Size:       1052     565     447

  Fragments arranged by size: 

                1052     565     447
 
PspOMI G'GGCC_C

Cuts at:      0     391     552    1600    2064 
   Size:        391     161    1048     464

  Fragments arranged by size: 

                1048     464     391     161
 
PspXI vC'TCGA_Gb

Cuts at:      0    1335    2064 
   Size:       1335     729
 
PstI C_TGCA'G

Cuts at:      0      41     142     674    1268    1355    1411    1886    1896 
   Size:         41     101     532     594      87      56     475      10

Cuts at:   1896    2064 
   Size:        168

  Fragments arranged by size: 

                 594     532     475     168     101      87      56      41
                  10
 
PvuII CAG'CTG

Cuts at:      0     669     799     832    1269    2038    2064 
   Size:        669     130      33     437     769      26

  Fragments arranged by size: 

                 769     669     437     130      33      26
 
SacI G_AGCT'C

Cuts at:      0      47    2064 
   Size:         47    2017
 
SacII CC_GC'GG

Cuts at:      0    1825    2064 
   Size:       1825     239
 
ScaI AGT'ACT

Cuts at:      0      73    2064 
   Size:         73    1991
 
SfcI C'TryA_G

Cuts at:      0      37     138     670    1264    1351    1407    1882    1892 
   Size:         37     101     532     594      87      56     475      10

Cuts at:   1892    2064 
   Size:        172

  Fragments arranged by size: 

                 594     532     475     172     101      87      56      37
                  10
 
SmlI C'TyrA_G

Cuts at:      0     604    1335    2064 
   Size:        604     731     729

  Fragments arranged by size: 

                 731     729     604
 
SphI G_CATG'C

Cuts at:      0    1226    2064 
   Size:       1226     838
 
SspI AAT'ATT

Cuts at:      0     910    2064 
   Size:        910    1154
 
StuI AGG'CCT

Cuts at:      0     586    2064 
   Size:        586    1478
 
StyI C'CwwG_G

Cuts at:      0     640    2064 
   Size:        640    1424
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1438    1555    2064 
   Size:       1438     117     509

  Fragments arranged by size: 

                1438     509     117
 
TatI w'GTAC_w

Cuts at:      0      71    2064 
   Size:         71    1993
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      90     354    2064 
   Size:         90     264    1710

  Fragments arranged by size: 

                1710     264      90
 
XhoI C'TCGA_G

Cuts at:      0    1335    2064 
   Size:       1335     729
 
XmnI GAAnn'nnTTC

Cuts at:      0    1474    2064 
   Size:       1474     590
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
    CspCI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FseI     FspI    HaeII    Hin4I  HindIII     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PasI
     PciI     PfoI     PmlI   PspOMI    PspXI     PstI    PvuII     SacI
    SacII     ScaI     SfcI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BcgI    BciVI
     BmtI     BsaI    BsaWI    BsaXI    BsiWI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI
     DraI     DrdI     EagI    EcoRI    EcoRV    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     PacI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SwaI     TstI  Tth111I     XbaI     XmaI     ZraI
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