(Linear) (Six-base) MAPSORT of: KIBB6263.seq Check: 7381 from: 1 to: 2064 KIBB6263.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 361 1457 1784 2064 Size: 361 1096 327 280 Fragments arranged by size: 1096 361 327 280 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 338 598 677 1545 2064 Size: 338 260 79 868 519 Fragments arranged by size: 868 519 338 260 79 AfeI AGC'GCT Cuts at: 0 1585 2064 Size: 1585 479 AflIII A'CryG_T Cuts at: 0 734 883 1392 2064 Size: 734 149 509 672 Fragments arranged by size: 734 672 509 149 AhdI GACnn_n'nnGTC Cuts at: 0 470 2064 Size: 470 1594 AleI CACnn'nnGTG Cuts at: 0 122 1292 1397 1463 1490 2064 Size: 122 1170 105 66 27 574 Fragments arranged by size: 1170 574 122 105 66 27 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1907 2064 Size: 1907 157 AlwNI CAG_nnn'CTG Cuts at: 0 1977 2041 2064 Size: 1977 64 23 Fragments arranged by size: 1977 64 23 ApaI G_GGCC'C Cuts at: 0 395 556 1604 2064 Size: 395 161 1048 460 Fragments arranged by size: 1048 460 395 161 ApaLI G'TGCA_C Cuts at: 0 1099 1699 1737 2064 Size: 1099 600 38 327 Fragments arranged by size: 1099 600 327 38 AvaI C'yCGr_G Cuts at: 0 619 1335 2047 2064 Size: 619 716 712 17 Fragments arranged by size: 716 712 619 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 848 881 2064 Size: 848 33 1183 Fragments arranged by size: 1183 848 33 BanI G'GyrC_C Cuts at: 0 661 864 1325 2064 Size: 661 203 461 739 Fragments arranged by size: 739 661 461 203 BanII G_rGCy'C Cuts at: 0 47 395 497 556 823 1209 1604 1912 Size: 47 348 102 59 267 386 395 308 Cuts at: 1912 2021 2064 Size: 109 43 Fragments arranged by size: 395 386 348 308 267 109 102 59 47 43 BbsI GAAGACnn'nnnn_ Cuts at: 0 1038 2064 Size: 1038 1026 BbvCI CC'TCA_GC Cuts at: 0 901 2064 Size: 901 1163 BclI T'GATC_A Cuts at: 0 1190 1847 2064 Size: 1190 657 217 Fragments arranged by size: 1190 657 217 BfrBI ATG'CAT Cuts at: 0 1222 2064 Size: 1222 842 BglI GCCn_nnn'nGGC Cuts at: 0 642 1346 2064 Size: 642 704 718 Fragments arranged by size: 718 704 642 BglII A'GATC_T Cuts at: 0 212 2064 Size: 212 1852 BlpI GC'TnA_GC Cuts at: 0 800 833 2064 Size: 800 33 1231 Fragments arranged by size: 1231 800 33 Bme1580I G_kGCm'C Cuts at: 0 395 556 638 666 1103 1328 1604 1703 Size: 395 161 82 28 437 225 276 99 Cuts at: 1703 1741 2064 Size: 38 323 Fragments arranged by size: 437 395 323 276 225 161 99 82 38 28 BmgBI CAC'GTC Cuts at: 0 93 423 1704 2064 Size: 93 330 1281 360 Fragments arranged by size: 1281 360 330 93 BmrI ACTGGGnnnn_n' Cuts at: 0 950 2064 Size: 950 1114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 573 1926 2064 Size: 573 1353 138 Fragments arranged by size: 1353 573 138 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 1024 1921 2035 2064 Size: 141 883 897 114 29 Fragments arranged by size: 897 883 141 114 29 Bpu10I CC'TnA_GC Cuts at: 0 174 811 901 2064 Size: 174 637 90 1163 Fragments arranged by size: 1163 637 174 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 625 2064 Size: 625 1439 BsaAI yAC'GTr Cuts at: 0 1052 1395 1617 2064 Size: 1052 343 222 447 Fragments arranged by size: 1052 447 343 222 BsaBI GATnn'nnATC Cuts at: 0 1360 2064 Size: 1360 704 BsaHI Gr'CG_yC Cuts at: 0 80 2064 Size: 80 1984 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 549 1330 2002 2064 Size: 549 781 672 62 Fragments arranged by size: 781 672 549 62 BseYI C'CCAG_C Cuts at: 0 387 524 537 665 1151 1958 2064 Size: 387 137 13 128 486 807 106 Fragments arranged by size: 807 486 387 137 128 106 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 815 2003 2064 Size: 815 1188 61 Fragments arranged by size: 1188 815 61 BsiEI CG_ry'CG Cuts at: 0 872 1803 2064 Size: 872 931 261 Fragments arranged by size: 931 872 261 BsiHKAI G_wGCw'C Cuts at: 0 47 287 1057 1103 1703 1741 2064 Size: 47 240 770 46 600 38 323 Fragments arranged by size: 770 600 323 240 47 46 38 BsmI GAATG_Cn' Cuts at: 0 880 2064 Size: 880 1184 Bsp1286I G_dGCh'C Cuts at: 0 47 287 395 497 556 638 666 823 Size: 47 240 108 102 59 82 28 157 Cuts at: 823 1057 1103 1209 1328 1604 1703 1741 1912 Size: 234 46 106 119 276 99 38 171 Cuts at: 1912 2021 2064 Size: 109 43 Fragments arranged by size: 276 240 234 171 157 119 109 108 106 102 99 82 59 47 46 43 38 28 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 361 1457 1784 2064 Size: 361 1096 327 280 Fragments arranged by size: 1096 361 327 280 BsrBI CCG'CTC Cuts at: 0 1034 2064 Size: 1034 1030 BsrFI r'CCGG_y Cuts at: 0 432 867 1640 1999 2064 Size: 432 435 773 359 65 Fragments arranged by size: 773 435 432 359 65 BstAPI GCAn_nnn'nTGC Cuts at: 0 1161 2064 Size: 1161 903 BstEII G'GTnAC_C Cuts at: 0 247 2064 Size: 247 1817 BstXI CCAn_nnnn'nTGG Cuts at: 0 1492 2064 Size: 1492 572 BstYI r'GATC_y Cuts at: 0 212 1793 2064 Size: 212 1581 271 Fragments arranged by size: 1581 271 212 Bsu36I CC'TnA_GG Cuts at: 0 264 746 1609 2064 Size: 264 482 863 455 Fragments arranged by size: 863 482 455 264 BtgI C'CryG_G Cuts at: 0 1290 1682 1822 2064 Size: 1290 392 140 242 Fragments arranged by size: 1290 392 242 140 BtsI GCAGTG_nn' Cuts at: 0 189 2064 Size: 189 1875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1272 1307 2064 Size: 1272 35 757 Fragments arranged by size: 1272 757 35 DraIII CAC_nnn'GTG Cuts at: 0 27 1107 1745 2064 Size: 27 1080 638 319 Fragments arranged by size: 1080 638 319 27 EaeI y'GGCC_r Cuts at: 0 375 1619 1968 2064 Size: 375 1244 349 96 Fragments arranged by size: 1244 375 349 96 EarI CTCTTCn'nnn_ Cuts at: 0 443 2064 Size: 443 1621 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1066 1930 2064 Size: 1066 864 134 Fragments arranged by size: 1066 864 134 EcoICRI GAG'CTC Cuts at: 0 45 2064 Size: 45 2019 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 338 598 677 1024 1545 1921 2035 Size: 141 197 260 79 347 521 376 114 Cuts at: 2035 2064 Size: 29 Fragments arranged by size: 521 376 347 260 197 141 114 79 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 1939 2064 Size: 1939 125 EcoO109I rG'GnC_Cy Cuts at: 0 99 391 392 521 552 553 644 1556 Size: 99 292 1 129 31 1 91 912 Cuts at: 1556 1600 1601 2064 Size: 44 1 463 Fragments arranged by size: 912 463 292 129 99 91 44 31 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 603 999 1191 2064 Size: 603 396 192 873 Fragments arranged by size: 873 603 396 192 FseI GG_CCGG'CC Cuts at: 0 1644 2064 Size: 1644 420 FspI TGC'GCA Cuts at: 0 1453 2064 Size: 1453 611 HaeII r_GCGC'y Cuts at: 0 1587 1882 2064 Size: 1587 295 182 Fragments arranged by size: 1587 295 182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 456 488 721 753 2064 Size: 456 32 233 32 1311 Fragments arranged by size: 1311 456 233 32 32 HindIII A'AGCT_T Cuts at: 0 1277 2064 Size: 1277 787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 209 2064 Size: 209 1855 MscI TGG'CCA Cuts at: 0 377 1621 2064 Size: 377 1244 443 Fragments arranged by size: 1244 443 377 MslI CAynn'nnrTG Cuts at: 0 122 1292 1379 1395 1397 1463 1490 2064 Size: 122 1170 87 16 2 66 27 574 Fragments arranged by size: 1170 574 122 87 66 27 16 2 MspA1I CmG'CkG Cuts at: 0 57 669 799 832 862 1269 1824 2038 Size: 57 612 130 33 30 407 555 214 Cuts at: 2038 2064 Size: 26 Fragments arranged by size: 612 555 407 214 130 57 33 30 26 NaeI GCC'GGC Cuts at: 0 434 1642 2064 Size: 434 1208 422 Fragments arranged by size: 1208 434 422 NgoMIV G'CCGG_C Cuts at: 0 432 1640 2064 Size: 432 1208 424 Fragments arranged by size: 1208 432 424 NsiI A_TGCA'T Cuts at: 0 1224 2064 Size: 1224 840 NspI r_CATG'y Cuts at: 0 738 887 1226 1446 2064 Size: 738 149 339 220 618 Fragments arranged by size: 738 618 339 220 149 PasI CC'CwG_GG Cuts at: 0 482 1941 2064 Size: 482 1459 123 Fragments arranged by size: 1459 482 123 PciI A'CATG_T Cuts at: 0 734 883 2064 Size: 734 149 1181 Fragments arranged by size: 1181 734 149 PfoI T'CCnGG_A Cuts at: 0 1001 2012 2064 Size: 1001 1011 52 Fragments arranged by size: 1011 1001 52 PmlI CAC'GTG Cuts at: 0 1052 1617 2064 Size: 1052 565 447 Fragments arranged by size: 1052 565 447 PspOMI G'GGCC_C Cuts at: 0 391 552 1600 2064 Size: 391 161 1048 464 Fragments arranged by size: 1048 464 391 161 PspXI vC'TCGA_Gb Cuts at: 0 1335 2064 Size: 1335 729 PstI C_TGCA'G Cuts at: 0 41 142 674 1268 1355 1411 1886 1896 Size: 41 101 532 594 87 56 475 10 Cuts at: 1896 2064 Size: 168 Fragments arranged by size: 594 532 475 168 101 87 56 41 10 PvuII CAG'CTG Cuts at: 0 669 799 832 1269 2038 2064 Size: 669 130 33 437 769 26 Fragments arranged by size: 769 669 437 130 33 26 SacI G_AGCT'C Cuts at: 0 47 2064 Size: 47 2017 SacII CC_GC'GG Cuts at: 0 1825 2064 Size: 1825 239 ScaI AGT'ACT Cuts at: 0 73 2064 Size: 73 1991 SfcI C'TryA_G Cuts at: 0 37 138 670 1264 1351 1407 1882 1892 Size: 37 101 532 594 87 56 475 10 Cuts at: 1892 2064 Size: 172 Fragments arranged by size: 594 532 475 172 101 87 56 37 10 SmlI C'TyrA_G Cuts at: 0 604 1335 2064 Size: 604 731 729 Fragments arranged by size: 731 729 604 SphI G_CATG'C Cuts at: 0 1226 2064 Size: 1226 838 SspI AAT'ATT Cuts at: 0 910 2064 Size: 910 1154 StuI AGG'CCT Cuts at: 0 586 2064 Size: 586 1478 StyI C'CwwG_G Cuts at: 0 640 2064 Size: 640 1424 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1438 1555 2064 Size: 1438 117 509 Fragments arranged by size: 1438 509 117 TatI w'GTAC_w Cuts at: 0 71 2064 Size: 71 1993 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 90 354 2064 Size: 90 264 1710 Fragments arranged by size: 1710 264 90 XhoI C'TCGA_G Cuts at: 0 1335 2064 Size: 1335 729 XmnI GAAnn'nnTTC Cuts at: 0 1474 2064 Size: 1474 590 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI BaeI BanI BanII BbsI BbvCI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PasI PciI PfoI PmlI PspOMI PspXI PstI PvuII SacI SacII ScaI SfcI SmlI SphI SspI StuI StyI TaqII TatI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AloI ApoI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BmtI BsaI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI DrdI EagI EcoRI EcoRV FspAI HincII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI PacI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XmaI ZraI