(Circular) (Six-base) MAPSORT of: pFN21AB5859.seq Check: 1969 from: 1 to: 6085 pFN21AB5859 6085 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3895 278 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 1937 Size: 1491 4594 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2694 3261 4148 5532 5800 1632 Size: 1062 567 887 1384 268 1917 Fragments arranged by size: 1917 1384 1062 887 567 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 2492 4984 5961 1203 Size: 1289 2492 977 1327 Fragments arranged by size: 2492 1327 1289 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 2630 1166 Size: 1464 4621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AlwNI CAG_nnn'CTG Cuts at: 2315 3364 3383 3828 5400 2315 Size: 1049 19 445 1572 3000 Fragments arranged by size: 3000 1572 1049 445 19 ApaLI G'TGCA_C Cuts at: 4142 5298 4142 Size: 1156 4929 ApoI r'AATT_y Cuts at: 1493 2279 3394 3620 3772 5881 1493 Size: 786 1115 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 786 226 152 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 3410 1870 Size: 73 1467 4545 Fragments arranged by size: 4545 1467 73 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 3070 3406 4861 618 Size: 325 217 627 441 842 336 1455 1842 Fragments arranged by size: 1842 1455 842 627 441 336 325 217 BanII G_rGCy'C Cuts at: 1391 3404 1391 Size: 2013 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 2695 1548 Size: 1147 4938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 3502 3502 Size: 6085 Bme1580I G_kGCm'C Cuts at: 1790 2231 4146 5302 1790 Size: 441 1915 1156 2573 Fragments arranged by size: 2573 1915 1156 441 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2233 3816 4774 Size: 416 53 83 399 24 816 1583 958 Cuts at: 4774 442 Size: 1753 Fragments arranged by size: 1753 1583 958 816 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2792 2792 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4745 1239 Size: 3506 2579 Bpu10I CC'TnA_GC Cuts at: 2880 2880 Size: 6085 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2073 2440 2743 4210 5075 5373 2073 Size: 367 303 1467 865 298 2785 Fragments arranged by size: 2785 1467 865 367 303 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3328 4748 882 Size: 617 1829 1420 2219 Fragments arranged by size: 2219 1829 1420 617 BsaAI yAC'GTr Cuts at: 493 1204 2617 3876 493 Size: 711 1413 1259 2702 Fragments arranged by size: 2702 1413 1259 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3892 4274 4909 275 Size: 53 83 186 936 2359 382 635 1451 Fragments arranged by size: 2359 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5860 Fragments arranged by size: 5860 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2443 1186 Size: 1257 4828 BseYI C'CCAG_C Cuts at: 5288 5784 5288 Size: 496 5589 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2438 2907 1163 Size: 573 702 469 4341 Fragments arranged by size: 4341 702 573 469 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3535 4296 5324 1540 Size: 275 186 1534 761 1028 2301 Fragments arranged by size: 2301 1534 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4146 4231 5302 4146 Size: 85 1071 4929 Fragments arranged by size: 4929 1071 85 BsmI GAATG_Cn' Cuts at: 3596 3689 3596 Size: 93 5992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 3404 4146 4231 5302 1391 Size: 399 441 1173 742 85 1071 2174 Fragments arranged by size: 2174 1173 1071 742 441 399 85 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 3033 3974 5704 3033 Size: 941 1730 3414 Fragments arranged by size: 3414 1730 941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3440 844 Size: 2596 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3972 1394 Size: 429 2149 3507 Fragments arranged by size: 3507 2149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2661 2810 4580 4754 65 Size: 1183 1413 149 1770 174 1396 Fragments arranged by size: 1770 1413 1396 1183 174 149 BsrFI r'CCGG_y Cuts at: 1700 1810 4729 4916 1700 Size: 110 2919 187 2869 Fragments arranged by size: 2919 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 4145 5157 4145 Size: 1012 5073 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstXI CCAn_nnnn'nTGG Cuts at: 2856 2856 Size: 6085 BstYI r'GATC_y Cuts at: 1972 2260 3415 3782 4167 4184 5636 5722 5734 Size: 288 1155 367 385 17 1452 86 12 Cuts at: 5734 6080 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1155 385 367 346 288 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5533 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 2643 3601 4395 4415 2643 Size: 958 794 20 4313 Fragments arranged by size: 4313 958 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3262 3297 529 Size: 35 2698 35 3317 Fragments arranged by size: 3317 2698 35 35 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050 Size: 54 1425 50 275 18 1702 889 1629 Cuts at: 6050 8 Size: 43 Fragments arranged by size: 1702 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 1537 Size: 275 1720 4090 Fragments arranged by size: 4090 1720 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4015 1353 Size: 2662 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4672 5046 5192 387 Size: 1064 138 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2694 3261 4148 4745 5532 5800 1239 Size: 393 1062 567 887 597 787 268 1524 Fragments arranged by size: 1524 1062 887 787 597 567 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2091 2091 Size: 6085 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2198 2220 2198 Size: 22 6063 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 5232 1678 Size: 3554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2810 2842 4726 4758 4800 Size: 32 135 32 1238 32 1884 32 42 Cuts at: 4800 4832 1373 Size: 32 2626 Fragments arranged by size: 2626 1884 1238 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2662 2726 3429 3675 669 Size: 988 281 724 64 703 246 3079 Fragments arranged by size: 3079 988 724 703 281 246 64 HindIII A'AGCT_T Cuts at: 748 2645 2967 3078 748 Size: 1897 322 111 3755 Fragments arranged by size: 3755 1897 322 111 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3231 3684 3231 Size: 453 5632 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2032 5199 5383 1305 Size: 727 3167 184 2007 Fragments arranged by size: 3167 2007 727 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4263 Fragments arranged by size: 4263 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 4043 4402 4561 518 Size: 648 1464 1413 359 159 2042 Fragments arranged by size: 2042 1464 1413 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3161 3361 3501 4178 5326 5571 1606 Size: 1555 200 140 677 1148 245 2120 Fragments arranged by size: 2120 1555 1148 677 245 200 140 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5533 NdeI CA'TA_TG Cuts at: 387 2032 387 Size: 1645 4440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 2800 3443 4988 1132 Size: 97 1571 643 1545 2229 Fragments arranged by size: 2229 1571 1545 643 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PstI C_TGCA'G Cuts at: 830 3437 830 Size: 2607 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 3161 3361 3161 Size: 200 5885 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 2112 3433 4568 5249 5440 826 Size: 219 1067 1321 1135 681 191 1471 Fragments arranged by size: 1471 1321 1135 1067 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2088 2455 2758 4189 5090 5352 Size: 197 926 145 367 303 1431 901 262 Cuts at: 5352 820 Size: 1553 Fragments arranged by size: 1553 1431 926 901 367 303 262 197 145 SnaBI TAC'GTA Cuts at: 493 2617 3876 493 Size: 2124 1259 2702 Fragments arranged by size: 2702 2124 1259 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6085 StyI C'CwwG_G Cuts at: 513 1065 3524 513 Size: 552 2459 3074 Fragments arranged by size: 3074 2459 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3115 4112 4129 4282 4467 4912 325 Size: 1255 1535 997 17 153 185 445 1498 Fragments arranged by size: 1535 1498 1255 997 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3291 4331 6043 Size: 274 80 33 51 1443 1314 1040 1712 Cuts at: 6043 96 Size: 138 Fragments arranged by size: 1712 1443 1314 1040 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6045 6077 6045 Size: 32 6053 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6085 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2853 2853 Size: 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 4214 4214 Size: 6085 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3893 276 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI