(Circular) (Six-base) MAPSORT of: pFN21AE1484.seq Check: 8465 from: 1 to: 6670 pFN21AE1484 6670 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3965 4480 278 Size: 53 83 186 936 2429 515 2468 Fragments arranged by size: 2468 2429 936 515 186 83 53 AccI GT'mk_AC Cuts at: 1937 2759 4013 1937 Size: 822 1254 4594 Fragments arranged by size: 4594 1254 822 Acc65I G'GTAC_C Cuts at: 3991 3991 Size: 6670 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 2271 2825 4733 6117 6385 1632 Size: 483 156 554 1908 1384 268 1917 Fragments arranged by size: 1917 1908 1384 554 483 268 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 3091 5569 6546 1203 Size: 1888 2478 977 1327 Fragments arranged by size: 2478 1888 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 3092 1166 Size: 1926 4744 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2645 1184 Size: 1461 5209 AlwNI CAG_nnn'CTG Cuts at: 2474 3511 4413 5985 2474 Size: 1037 902 1572 3159 Fragments arranged by size: 3159 1572 1037 902 ApaLI G'TGCA_C Cuts at: 2972 4727 5883 2972 Size: 1755 1156 3759 Fragments arranged by size: 3759 1755 1156 ApoI r'AATT_y Cuts at: 1493 2225 2577 2833 2909 3306 3573 3979 4205 Size: 732 352 256 76 397 267 406 226 Cuts at: 4205 4357 6466 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 732 406 397 352 267 256 226 152 76 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 3995 1870 Size: 73 2052 4545 Fragments arranged by size: 4545 2052 73 BamHI G'GATC_C Cuts at: 4000 4367 4000 Size: 367 6303 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2536 3851 3991 5446 618 Size: 325 217 627 749 1315 140 1455 1842 Fragments arranged by size: 1842 1455 1315 749 627 325 217 140 BanII G_rGCy'C Cuts at: 1391 2145 3492 3989 1391 Size: 754 1347 497 4072 Fragments arranged by size: 4072 1347 754 497 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2778 3681 928 Size: 971 879 903 3917 Fragments arranged by size: 3917 971 903 879 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3918 3952 4861 4895 1903 Size: 34 1981 34 909 34 3678 Fragments arranged by size: 3678 1981 909 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4562 5772 4562 Size: 1210 5460 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3841 5281 136 Size: 107 122 71 631 605 2169 1440 1525 Fragments arranged by size: 2169 1525 1440 631 605 122 107 71 BglII A'GATC_T Cuts at: 3247 6665 3247 Size: 3418 3252 BlpI GC'TnA_GC Cuts at: 3041 4087 3041 Size: 1046 5624 Bme1580I G_kGCm'C Cuts at: 1790 2976 4731 5887 1790 Size: 1186 1755 1156 2573 Fragments arranged by size: 2573 1755 1186 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6670 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2034 4401 5359 Size: 416 53 83 399 24 617 2367 958 Cuts at: 5359 442 Size: 1753 Fragments arranged by size: 2367 1753 958 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3634 3637 3634 Size: 3 6667 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2304 3770 5330 1239 Size: 1065 1466 1560 2579 Fragments arranged by size: 2579 1560 1466 1065 Bpu10I CC'TnA_GC Cuts at: 3456 3456 Size: 6670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3902 4795 5660 5958 3902 Size: 893 865 298 4614 Fragments arranged by size: 4614 893 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2814 3176 5333 882 Size: 617 1315 362 2157 2219 Fragments arranged by size: 2219 2157 1315 617 362 BsaAI yAC'GTr Cuts at: 493 1204 2662 4461 493 Size: 711 1458 1799 2702 Fragments arranged by size: 2702 1799 1458 711 BsaBI GATnn'nnATC Cuts at: 6664 6664 Size: 6670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3876 3962 4477 4859 Size: 53 83 186 936 2343 86 515 382 Cuts at: 4859 5494 275 Size: 635 1451 Fragments arranged by size: 2343 1451 936 635 515 382 186 86 83 53 BsaWI w'CCGG_w Cuts at: 5103 5501 5775 5922 6333 5103 Size: 398 274 147 411 5440 Fragments arranged by size: 5440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3824 3854 1178 Size: 30 165 30 2421 30 3994 Fragments arranged by size: 3994 2421 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2768 3218 3500 3610 3626 3665 3668 3755 Size: 1582 450 282 110 16 39 3 87 Cuts at: 3755 3845 1186 Size: 90 4011 Fragments arranged by size: 4011 1582 450 282 110 90 87 39 16 3 BseYI C'CCAG_C Cuts at: 2087 2348 3941 5873 6369 2087 Size: 261 1593 1932 496 2388 Fragments arranged by size: 2388 1932 1593 496 261 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2924 3074 3791 1163 Size: 573 1188 150 717 4042 Fragments arranged by size: 4042 1188 717 573 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4120 4881 5909 1540 Size: 275 186 1597 522 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 522 275 186 BsiHKAI G_wGCw'C Cuts at: 2976 3042 3492 4731 4816 5887 2976 Size: 66 450 1239 85 1071 3759 Fragments arranged by size: 3759 1239 1071 450 85 66 BsmI GAATG_Cn' Cuts at: 2622 4181 4274 2622 Size: 1559 93 5018 Fragments arranged by size: 5018 1559 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3864 3953 3864 Size: 89 6581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2976 3042 3492 3989 4731 4816 Size: 399 355 831 66 450 497 742 85 Cuts at: 4816 5887 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 831 742 497 450 399 355 85 66 BspEI T'CCGG_A Cuts at: 6333 6333 Size: 6670 BspHI T'CATG_A Cuts at: 3736 4559 6289 3736 Size: 823 1730 4117 Fragments arranged by size: 4117 1730 823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4025 844 Size: 3181 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4557 1394 Size: 429 2734 3507 Fragments arranged by size: 3507 2734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2988 5165 5339 65 Size: 1183 1740 2177 174 1396 Fragments arranged by size: 2177 1740 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3516 3925 5314 5501 1700 Size: 110 1706 409 1389 187 2869 Fragments arranged by size: 2869 1706 1389 409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 4730 5742 4730 Size: 1012 5658 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 3511 794 Size: 2021 696 3953 Fragments arranged by size: 3953 2021 696 BstBI TT'CG_AA Cuts at: 5474 5474 Size: 6670 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6670 BstYI r'GATC_y Cuts at: 1972 3247 3468 4000 4367 4752 4769 6221 6307 Size: 1275 221 532 367 385 17 1452 86 Cuts at: 6307 6319 6665 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1275 532 385 367 346 221 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2886 3498 513 Size: 552 1821 612 3685 Fragments arranged by size: 3685 1821 612 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3160 3921 487 Size: 683 56 1934 761 3236 Fragments arranged by size: 3236 1934 761 683 56 BtsI GCAGTG_nn' Cuts at: 4186 4980 5000 4186 Size: 794 20 5856 Fragments arranged by size: 5856 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 2380 3974 2380 Size: 1594 5076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3159 3495 3595 Size: 54 1425 50 275 18 1329 336 100 Cuts at: 3595 4117 5006 6635 8 Size: 522 889 1629 43 Fragments arranged by size: 1629 1425 1329 889 522 336 275 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 4117 1537 Size: 275 1783 522 4090 Fragments arranged by size: 4090 1783 522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 2309 2862 3408 3534 3690 4600 1353 Size: 738 218 553 546 126 156 910 3423 Fragments arranged by size: 3423 910 738 553 546 218 156 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5257 5631 5777 387 Size: 1064 138 3668 374 146 1280 Fragments arranged by size: 3668 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3490 3490 Size: 6670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2271 2304 2825 3770 4733 5330 Size: 393 483 156 33 521 945 963 597 Cuts at: 5330 6117 6385 1239 Size: 787 268 1524 Fragments arranged by size: 1524 963 945 787 597 521 483 393 268 156 33 EcoNI CCTnn'n_nnAGG Cuts at: 3455 3455 Size: 6670 EcoRI G'AATT_C Cuts at: 2577 2909 3979 4357 2577 Size: 332 1070 378 4890 Fragments arranged by size: 4890 1070 378 332 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 6670 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 5817 1678 Size: 4139 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2438 2470 3049 Size: 32 135 32 599 32 235 32 579 Cuts at: 3049 3081 3124 3156 3370 3402 3861 3893 5311 Size: 32 43 32 214 32 459 32 1418 Cuts at: 5311 5343 5385 5417 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1418 599 579 459 235 214 135 43 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4014 4260 669 Size: 988 281 2076 246 3079 Fragments arranged by size: 3079 2076 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6670 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KpnI G_GTAC'C Cuts at: 3995 3995 Size: 6670 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5784 5968 1305 Size: 4479 184 2007 Fragments arranged by size: 4479 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3161 3497 10 Size: 54 1425 343 1329 336 3183 Fragments arranged by size: 3183 1425 1329 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 3092 4628 4987 5146 518 Size: 648 1926 1536 359 159 2042 Fragments arranged by size: 2042 1926 1536 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2070 2474 3775 4086 4763 5911 6156 1606 Size: 464 404 1301 311 677 1148 245 2120 Fragments arranged by size: 2120 1301 1148 677 464 404 311 245 NaeI GCC'GGC Cuts at: 3518 3927 3518 Size: 409 6261 NcoI C'CATG_G Cuts at: 513 1065 3498 513 Size: 552 2433 3685 Fragments arranged by size: 3685 2433 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NgoMIV G'CCGG_C Cuts at: 3516 3925 3516 Size: 409 6261 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 3095 4028 5573 1132 Size: 97 1866 933 1545 2229 Fragments arranged by size: 2229 1866 1545 933 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6670 PciI A'CATG_T Cuts at: 3091 5569 3091 Size: 2478 4192 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6670 PfoI T'CCnGG_A Cuts at: 2119 2119 Size: 6670 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4739 4771 6252 6284 4739 Size: 32 1481 32 5125 Fragments arranged by size: 5125 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 3440 4240 3440 Size: 800 5870 PstI C_TGCA'G Cuts at: 830 3255 3663 4022 830 Size: 2425 408 359 3478 Fragments arranged by size: 3478 2425 408 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 PvuII CAG'CTG Cuts at: 2474 3775 2474 Size: 1301 5369 SacI G_AGCT'C Cuts at: 3492 3492 Size: 6670 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SapI GCTCTTCn'nnn_ Cuts at: 3690 3690 Size: 6670 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 3617 4918 6630 3617 Size: 1301 1712 3657 Fragments arranged by size: 3657 1712 1301 SfcI C'TryA_G Cuts at: 826 1045 2695 2790 3033 3251 3659 4018 5153 Size: 219 1650 95 243 218 408 359 1135 Cuts at: 5153 5834 6025 826 Size: 681 191 1471 Fragments arranged by size: 1650 1471 1135 681 408 359 243 219 218 191 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 3997 1872 Size: 2125 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3881 4774 5675 5937 820 Size: 197 926 1938 893 901 262 1553 Fragments arranged by size: 1938 1553 926 901 893 262 197 SnaBI TAC'GTA Cuts at: 493 2662 4461 493 Size: 2169 1799 2702 Fragments arranged by size: 2702 2169 1799 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 4028 1132 Size: 2896 3774 SspI AAT'ATT Cuts at: 5 52 3445 4594 5 Size: 47 3393 1149 2081 Fragments arranged by size: 3393 2081 1149 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6670 StyI C'CwwG_G Cuts at: 513 1065 2322 3498 3801 4109 513 Size: 552 1257 1176 303 308 3074 Fragments arranged by size: 3074 1257 1176 552 308 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4697 4714 4867 5052 5497 325 Size: 1255 3117 17 153 185 445 1498 Fragments arranged by size: 3117 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3298 3615 4916 Size: 274 80 33 51 1443 1321 317 1301 Cuts at: 4916 6628 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1321 1301 317 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3824 3856 6630 6662 3824 Size: 32 2774 32 3832 Fragments arranged by size: 3832 2774 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2984 3875 1316 Size: 1668 891 4111 Fragments arranged by size: 4111 1668 891 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 3995 1870 Size: 2125 4545 XmnI GAAnn'nnTTC Cuts at: 4799 4799 Size: 6670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3963 4478 276 Size: 53 83 186 936 2429 515 2468 Fragments arranged by size: 2468 2429 936 515 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI