(Linear) (Six-base) MAPSORT of: KIEE1484.seq Check: 1056 from: 1 to: 1965 KIEE1484.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AatII G_ACGT'C Cuts at: 0 1960 1965 Size: 1960 5 AccI GT'mk_AC Cuts at: 0 754 1965 Size: 754 1211 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 266 820 1965 Size: 110 156 554 1145 Fragments arranged by size: 1145 554 156 110 AflIII A'CryG_T Cuts at: 0 1086 1965 Size: 1086 879 AleI CACnn'nnGTG Cuts at: 0 1087 1965 Size: 1087 878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 640 1965 Size: 640 1325 AlwNI CAG_nnn'CTG Cuts at: 0 469 1506 1965 Size: 469 1037 459 Fragments arranged by size: 1037 469 459 ApaLI G'TGCA_C Cuts at: 0 967 1965 Size: 967 998 ApoI r'AATT_y Cuts at: 0 220 572 828 904 1301 1568 1965 Size: 220 352 256 76 397 267 397 Fragments arranged by size: 397 397 352 267 256 220 76 BanI G'GyrC_C Cuts at: 0 531 1846 1965 Size: 531 1315 119 Fragments arranged by size: 1315 531 119 BanII G_rGCy'C Cuts at: 0 140 1487 1965 Size: 140 1347 478 Fragments arranged by size: 1347 478 140 BbsI GAAGACnn'nnnn_ Cuts at: 0 773 1676 1965 Size: 773 903 289 Fragments arranged by size: 903 773 289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1913 1947 1965 Size: 1913 34 18 Fragments arranged by size: 1913 34 18 BglI GCCn_nnn'nGGC Cuts at: 0 1836 1965 Size: 1836 129 BglII A'GATC_T Cuts at: 0 1242 1965 Size: 1242 723 BlpI GC'TnA_GC Cuts at: 0 1036 1965 Size: 1036 929 Bme1580I G_kGCm'C Cuts at: 0 971 1965 Size: 971 994 BmrI ACTGGGnnnn_n' Cuts at: 0 29 1965 Size: 29 1936 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1629 1632 1965 Size: 1629 3 333 Fragments arranged by size: 1629 333 3 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 299 1765 1965 Size: 299 1466 200 Fragments arranged by size: 1466 299 200 Bpu10I CC'TnA_GC Cuts at: 0 1451 1965 Size: 1451 514 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1897 1965 Size: 1897 68 BsaI GGTCTCn'nnnn_ Cuts at: 0 809 1171 1965 Size: 809 362 794 Fragments arranged by size: 809 794 362 BsaAI yAC'GTr Cuts at: 0 657 1965 Size: 657 1308 BsaHI Gr'CG_yC Cuts at: 0 1871 1957 1965 Size: 1871 86 8 Fragments arranged by size: 1871 86 8 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1819 1849 1965 Size: 1819 30 116 Fragments arranged by size: 1819 116 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 763 1213 1495 1605 1621 1660 1663 1750 Size: 763 450 282 110 16 39 3 87 Cuts at: 1750 1840 1965 Size: 90 125 Fragments arranged by size: 763 450 282 125 110 90 87 39 16 3 BseYI C'CCAG_C Cuts at: 0 82 343 1936 1965 Size: 82 261 1593 29 Fragments arranged by size: 1593 261 82 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 919 1069 1786 1965 Size: 919 150 717 179 Fragments arranged by size: 919 717 179 150 BsiEI CG_ry'CG Cuts at: 0 1593 1965 Size: 1593 372 BsiHKAI G_wGCw'C Cuts at: 0 971 1037 1487 1965 Size: 971 66 450 478 Fragments arranged by size: 971 478 450 66 BsmI GAATG_Cn' Cuts at: 0 617 1965 Size: 617 1348 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1859 1948 1965 Size: 1859 89 17 Fragments arranged by size: 1859 89 17 Bsp1286I G_dGCh'C Cuts at: 0 140 971 1037 1487 1965 Size: 140 831 66 450 478 Fragments arranged by size: 831 478 450 140 66 BspHI T'CATG_A Cuts at: 0 1731 1965 Size: 1731 234 BsrDI GCAATG_nn' Cuts at: 0 983 1965 Size: 983 982 BsrFI r'CCGG_y Cuts at: 0 1511 1920 1965 Size: 1511 409 45 Fragments arranged by size: 1511 409 45 BstAPI GCAn_nnn'nTGC Cuts at: 0 810 1506 1965 Size: 810 696 459 Fragments arranged by size: 810 696 459 BstYI r'GATC_y Cuts at: 0 1242 1463 1965 Size: 1242 221 502 Fragments arranged by size: 1242 502 221 BtgI C'CryG_G Cuts at: 0 881 1493 1965 Size: 881 612 472 Fragments arranged by size: 881 612 472 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1155 1916 1965 Size: 1155 761 49 Fragments arranged by size: 1155 761 49 DraI TTT'AAA Cuts at: 0 375 1965 Size: 375 1590 EaeI y'GGCC_r Cuts at: 0 1154 1490 1590 1965 Size: 1154 336 100 375 Fragments arranged by size: 1154 375 336 100 EagI C'GGCC_G Cuts at: 0 1590 1965 Size: 1590 375 EarI CTCTTCn'nnn_ Cuts at: 0 86 304 857 1403 1529 1685 1965 Size: 86 218 553 546 126 156 280 Fragments arranged by size: 553 546 280 218 156 126 86 EcoICRI GAG'CTC Cuts at: 0 1485 1965 Size: 1485 480 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 266 299 820 1765 1965 Size: 110 156 33 521 945 200 Fragments arranged by size: 945 521 200 156 110 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 1450 1965 Size: 1450 515 EcoRI G'AATT_C Cuts at: 0 572 904 1965 Size: 572 332 1061 Fragments arranged by size: 1061 572 332 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 363 1965 Size: 363 1602 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 166 198 433 465 1044 1076 1119 1151 Size: 166 32 235 32 579 32 43 32 Cuts at: 1151 1365 1397 1856 1888 1965 Size: 214 32 459 32 77 Fragments arranged by size: 579 459 235 214 166 77 43 32 32 32 32 32 32 MscI TGG'CCA Cuts at: 0 1156 1492 1965 Size: 1156 336 473 Fragments arranged by size: 1156 473 336 MslI CAynn'nnrTG Cuts at: 0 1087 1965 Size: 1087 878 MspA1I CmG'CkG Cuts at: 0 65 469 1770 1965 Size: 65 404 1301 195 Fragments arranged by size: 1301 404 195 65 NaeI GCC'GGC Cuts at: 0 1513 1922 1965 Size: 1513 409 43 Fragments arranged by size: 1513 409 43 NcoI C'CATG_G Cuts at: 0 1493 1965 Size: 1493 472 NgoMIV G'CCGG_C Cuts at: 0 1511 1920 1965 Size: 1511 409 45 Fragments arranged by size: 1511 409 45 NspI r_CATG'y Cuts at: 0 1090 1965 Size: 1090 875 PciI A'CATG_T Cuts at: 0 1086 1965 Size: 1086 879 PfoI T'CCnGG_A Cuts at: 0 114 1965 Size: 114 1851 PsiI TTA'TAA Cuts at: 0 1435 1965 Size: 1435 530 PstI C_TGCA'G Cuts at: 0 1250 1658 1965 Size: 1250 408 307 Fragments arranged by size: 1250 408 307 PvuII CAG'CTG Cuts at: 0 469 1770 1965 Size: 469 1301 195 Fragments arranged by size: 1301 469 195 SacI G_AGCT'C Cuts at: 0 1487 1965 Size: 1487 478 SapI GCTCTTCn'nnn_ Cuts at: 0 1685 1965 Size: 1685 280 ScaI AGT'ACT Cuts at: 0 1612 1965 Size: 1612 353 SfcI C'TryA_G Cuts at: 0 690 785 1028 1246 1654 1965 Size: 690 95 243 218 408 311 Fragments arranged by size: 690 408 311 243 218 95 SmlI C'TyrA_G Cuts at: 0 1876 1965 Size: 1876 89 SnaBI TAC'GTA Cuts at: 0 657 1965 Size: 657 1308 SspI AAT'ATT Cuts at: 0 1440 1965 Size: 1440 525 StyI C'CwwG_G Cuts at: 0 317 1493 1796 1965 Size: 317 1176 303 169 Fragments arranged by size: 1176 317 303 169 TatI w'GTAC_w Cuts at: 0 1293 1610 1965 Size: 1293 317 355 Fragments arranged by size: 1293 355 317 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1819 1851 1965 Size: 1819 32 114 Fragments arranged by size: 1819 114 32 Tth111I GACn'n_nGTC Cuts at: 0 979 1870 1965 Size: 979 891 95 Fragments arranged by size: 979 891 95 ZraI GAC'GTC Cuts at: 0 1958 1965 Size: 1958 7 Enzymes that do cut: AatII AccI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BstAPI BstYI BtgI BtgZI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PfoI PsiI PstI PvuII SacI SapI ScaI SfcI SmlI SnaBI SspI StyI TatI TstI Tth111I ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI BsaBI BsaWI BsiWI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraIII DrdI EciI EcoO109I EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI TaqII XbaI XcmI XhoI XmaI XmnI