Restriction map for
pFN21AE0908
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI AlwI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcyI 445 GGCGTC G[GA]^CG[CT]C
Alw44I 45 GTGCAC G^TGCAC
ApaI 195 GGGCCC GGGCC^C
ApaLI 45 GTGCAC G^TGCAC
BanI 59 GGCACC G^G[CT][GA]CC
BcgI 13 GCAGGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 379 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 379 GCTGAGC GC^T[ACGT]AGC
BsaHI 445 GGCGTC G[GA]^CG[CT]C
BsmAI 244 GAGAC GTCTC(1/5)
BsrDI 438 CATTGC GCAATG(2/0)
BsrFI 608 ACCGGC [GA]^CCGG[CT]
BssSI 21 CTCGTG CACGAG(-5/-1)
CelII 379 GCTGAGC GC^T[ACGT]AGC
Cfr10I 608 ACCGGC [GA]^CCGG[CT]
DdeI 380 CTGAG C^T[ACGT]AG
EaeI 593 CGGCCA [CT]^GGCC[GA]
EcoT14I 596 CCAAGG C^C[AT][AT]GG
HaeII 587 GGCGCT [GA]GCGC^[CT]
HapII 609 CCGG C^CGG
HgaI 446 GCGTC GACGC(5/10)
Hin1I 445 GGCGTC G[GA]^CG[CT]C
HincII 265 GTTGAC GT[CT]^[GA]AC
HindII 265 GTTGAC GT[CT]^[GA]AC
HpaII 609 CCGG C^CGG
MspI 609 CCGG C^CGG
PvuII 638 CAGCTG CAG^CTG
SfaNI 624 GATGC GCATC(5/9)
SmlI 613 CTCAAG C^T[CT][GA]AG
StuI 323 AGGCCT AGG^CCT
StyI 596 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanII 195 GGGCCC G[GA]GC[CT]^C
662 GGGCTC
BglI 416 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
425 GCCTGCTGGGC
BsgI 408 CTGCAC GTGCAG(16/14)
652 CTGCAC
BstF5I 117 CATCC GGATG(2/0)
29 GGATG
CfoI 387 GCGC GCG^C
588 GCGC
FokI 117 CATCC GGATG(9/13)
29 GGATG
HhaI 387 GCGC GCG^C
588 GCGC
HinP1I 387 GCGC G^CGC
588 GCGC
HphI 137 TCACC GGTGA(8/7)
647 TCACC
MspA1I 481 CCGCTG C[AC]G^C[GT]G
638 CAGCTG
NlaIII 450 CATG CATG^
667 CATG
PflMI 100 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
537 CCAGAAGATGG
PpuMI 95 GGGACCC [GA]G^G[AT]CC[CT]
175 GGGACCT
Tsp45I 527 GTGAC ^GT[GC]AC
646 GTCAC
Van91I 100 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
537 CCAGAAGATGG
XcmI 260 CCACTGTTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
477 CCAGCCGCTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
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AciI AluI AlwNI AspHI AvaII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HinfI
ItaI MaeIII MboII MnlI MvaI
MwoI NlaIV PleI PstI Sau96I
ScrFI SfcI TseI TspRI