Restriction map for pFN21AE0908
 (Circular) (Six-base) MAPSORT of: pFN21AE0908.seq  Check: 2815  from: 1  to: 5380



pFN21AE0908  5380 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:37  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3190     278 
   Size:         53      83     186     936    1654    2468

  Fragments arranged by size: 

                2468    1654     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2723    1937 
   Size:        786    4594
 
Acc65I G'GTAC_C

Cuts at:   2701    2701 
   Size:       5380
 
AclI AA'CG_TT

Cuts at:   1563    3507    3880    1563 
   Size:       1944     373    3063

  Fragments arranged by size: 

                3063    1944     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2588    3443    4827    5095    1632 
   Size:        956     855    1384     268    1917

  Fragments arranged by size: 

                1917    1384     956     855     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5183
 
AflIII A'CryG_T

Cuts at:   1203    4279    5256    1203 
   Size:       3076     977    1327

  Fragments arranged by size: 

                3076    1327     977
 
AgeI A'CCGG_T

Cuts at:   4211    4211 
   Size:       5380
 
AhdI GACnn_n'nnGTC

Cuts at:   4109    4109 
   Size:       5380
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5380
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2088    1184 
   Size:        904    4476
 
AlwNI CAG_nnn'CTG

Cuts at:   2111    2431    2488    2645    3123    4695    2111 
   Size:        320      57     157     478    1572    2796

  Fragments arranged by size: 

                2796    1572     478     320     157      57
 
ApaI G_GGCC'C

Cuts at:   2204    2204 
   Size:       5380
 
ApaLI G'TGCA_C

Cuts at:   2050    3437    4593    2050 
   Size:       1387    1156    2837

  Fragments arranged by size: 

                2837    1387    1156
 
ApoI r'AATT_y

Cuts at:   1493    2689    2915    3067    5176    1493 
   Size:       1196     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1196     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3934     160 
   Size:        872    2902    1606

  Fragments arranged by size: 

                2902    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5380
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2705    1870 
   Size:         73     762    4545

  Fragments arranged by size: 

                4545     762      73
 
BamHI G'GATC_C

Cuts at:   2710    3077    2710 
   Size:        367    5013
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2064    2701    4156     618 
   Size:        325     217     627     277     637    1455    1842

  Fragments arranged by size: 

                1842    1455     637     627     325     277     217
 
BanII G_rGCy'C

Cuts at:   1391    2204    2671    2699    1391 
   Size:        813     467      28    4072

  Fragments arranged by size: 

                4072     813     467      28
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4409
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2007    2041    3571    3605    1903 
   Size:         34      70      34    1530      34    3678

  Fragments arranged by size: 

                3678    1530      70      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3272    4482    3272 
   Size:       1210    4170
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5380
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2427    2436    3991 
   Size:        107     122      71     631     605     755       9    1555

Cuts at:   3991     136 
   Size:       1525

  Fragments arranged by size: 

                1555    1525     755     631     605     122     107      71
                   9
 
BglII A'GATC_T

Cuts at:   5375    5375 
   Size:       5380
 
BlpI GC'TnA_GC

Cuts at:   2385    2797    2385 
   Size:        412    4968
 
Bme1580I G_kGCm'C

Cuts at:   1790    2054    2204    3441    4597    1790 
   Size:        264     150    1237    1156    2573

  Fragments arranged by size: 

                2573    1237    1156     264     150
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5380
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3111    4069     442 
   Size:        416      53      83     399      24    1694     958    1753

  Fragments arranged by size: 

                1753    1694     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5380
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2174    2408    2525    2622    4040    1239 
   Size:        935     234     117      97    1418    2579

  Fragments arranged by size: 

                2579    1418     935     234     117      97
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2603    3505    4370    4668    2603 
   Size:        902     865     298    3315

  Fragments arranged by size: 

                3315     902     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4043     882 
   Size:        617    2544    2219

  Fragments arranged by size: 

                2544    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3171     493 
   Size:        711    1967    2702

  Fragments arranged by size: 

                2702    1967     711
 
BsaBI GATnn'nnATC

Cuts at:   5374    5374 
   Size:       5380
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2451    3187    3569    4204 
   Size:         53      83     186     936     918     736     382     635

Cuts at:   4204     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     918     736     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3813    4211    4485    4632    5043    3813 
   Size:        398     274     147     411    4150

  Fragments arranged by size: 

                4150     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2497    2527    1178 
   Size:         30     165      30    1094      30    4031

  Fragments arranged by size: 

                4031    1094     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5380
 
BseYI C'CCAG_C

Cuts at:   2177    2434    2481    4583    5079    2177 
   Size:        257      47    2102     496    2478

  Fragments arranged by size: 

                2478    2102     496     257      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2398    2642    1163 
   Size:        573     662     244    3901

  Fragments arranged by size: 

                3901     662     573     244
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2830    3591    4619    1540 
   Size:        275     186     829     761    1028    2301

  Fragments arranged by size: 

                2301    1028     829     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2054    2061    2160    3441    3526    4597    2054 
   Size:          7      99    1281      85    1071    2837

  Fragments arranged by size: 

                2837    1281    1071      99      85       7
 
BsmI GAATG_Cn'

Cuts at:   2891    2984    2891 
   Size:         93    5287
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2054    2061    2160    2204    2671    2699    3441 
   Size:        399     264       7      99      44     467      28     742

Cuts at:   3441    3526    4597    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     467     399     264      99      85
                  44      28       7
 
BspEI T'CCGG_A

Cuts at:   5043    5043 
   Size:       5380
 
BspHI T'CATG_A

Cuts at:   3269    4999    3269 
   Size:       1730    3650
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2735     844 
   Size:       1891    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3267    1394 
   Size:        429    1444    3507

  Fragments arranged by size: 

                3507    1444     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2442    3875    4049      65 
   Size:       1183    1194    1433     174    1396

  Fragments arranged by size: 

                1433    1396    1194    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2613    4024    4211    1700 
   Size:        110     803    1411     187    2869

  Fragments arranged by size: 

                2869    1411     803     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5380
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5380
 
BssSI C'ACGA_G

Cuts at:   2026    3440    4452    2026 
   Size:       1414    1012    2954

  Fragments arranged by size: 

                2954    1414    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5380
 
BstBI TT'CG_AA

Cuts at:   4184    4184 
   Size:       5380
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5380
 
BstYI r'GATC_y

Cuts at:   1972    2710    3077    3462    3479    4931    5017    5029    5375 
   Size:        738     367     385      17    1452      86      12     346

Cuts at:   5375    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     738     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3889

  Fragments arranged by size: 

                3889     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4641

  Fragments arranged by size: 

                4641     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2082    2379    2474    2896    3690    3710    2082 
   Size:        297      95     422     794      20    3752

  Fragments arranged by size: 

                3752     794     422     297      95      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5380
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5345
 
DraI TTT'AAA

Cuts at:   2684    2684 
   Size:       5380
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4387     809 
   Size:        507    3071    1802

  Fragments arranged by size: 

                3071    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2598    2827    3716 
   Size:         54    1425      50     275      18     768     229     889

Cuts at:   3716    5345       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     768     275     229      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2827    1537 
   Size:        275    1015    4090

  Fragments arranged by size: 

                4090    1015     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3310    1353 
   Size:       1957    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3967    4341    4487     387 
   Size:       1064     138    2378     374     146    1280

  Fragments arranged by size: 

                2378    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2174    2408    2525    2588    2622    3443    4040 
   Size:        393     542     234     117      63      34     821     597

Cuts at:   4040    4827    5095    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     821     787     597     542     393     268     234
                 117      63      34
 
EcoO109I rG'GnC_Cy

Cuts at:   2101    2108    2181    2201    2428    2101 
   Size:          7      73      20     227    5053

  Fragments arranged by size: 

                5053     227      73      20       7
 
EcoRI G'AATT_C

Cuts at:   2689    3067    2689 
   Size:        378    5002
 
FspI TGC'GCA

Cuts at:   3886    3886 
   Size:       5380
 
HaeII r_GCGC'y

Cuts at:   1678    2596    4527    1678 
   Size:        918    1931    2531

  Fragments arranged by size: 

                2531    1931     918
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2620    2652    4021    4053    4095 
   Size:         32     135      32    1048      32    1369      32      42

Cuts at:   4095    4127    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1369    1048     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2272    2724    2970     669 
   Size:        988     281     334     452     246    3079

  Fragments arranged by size: 

                3079     988     452     334     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5380
 
HpaI GTT'AAC

Cuts at:   2970    2970 
   Size:       5380
 
KpnI G_GTAC'C

Cuts at:   2705    2705 
   Size:       5380
 
MfeI C'AATT_G

Cuts at:   2979    2979 
   Size:       5380
 
MluI A'CGCG_T

Cuts at:   5256    5256 
   Size:       5380
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4494    4678    1305 
   Size:       3189     184    2007

  Fragments arranged by size: 

                3189    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3558

  Fragments arranged by size: 

                3558    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3338    3697    3856     518 
   Size:        648    2172     359     159    2042

  Fragments arranged by size: 

                2172    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2488    2645    2796    3473    4621    4866    1606 
   Size:        882     157     151     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     882     677     245     157     151
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3889

  Fragments arranged by size: 

                3889     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5380
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5380
 
NotI GC'GGCC_GC

Cuts at:   2827    2827 
   Size:       5380
 
NspI r_CATG'y

Cuts at:   1132    1229    2738    4283    1132 
   Size:         97    1509    1545    2229

  Fragments arranged by size: 

                2229    1545    1509      97
 
PasI CC'CwG_GG

Cuts at:   1348    2105    2259    1348 
   Size:        757     154    4469

  Fragments arranged by size: 

                4469     757     154
 
PciI A'CATG_T

Cuts at:   4279    4279 
   Size:       5380
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2111    2548    1108 
   Size:       1003     437    3940

  Fragments arranged by size: 

                3940    1003     437
 
PmeI GTTT'AAAC

Cuts at:   2684    2684 
   Size:       5380
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3449    3481    4962    4994    3449 
   Size:         32    1481      32    3835

  Fragments arranged by size: 

                3835    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2101    2181    2101 
   Size:         80    5300
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5380
 
PsiI TTA'TAA

Cuts at:   2950    2950 
   Size:       5380
 
PspOMI G'GGCC_C

Cuts at:   2200    2200 
   Size:       5380
 
PstI C_TGCA'G

Cuts at:    830    2077    2580    2650    2732     830 
   Size:       1247     503      70      82    3478

  Fragments arranged by size: 

                3478    1247     503      82      70
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5380
 
PvuII CAG'CTG

Cuts at:   2645    2645 
   Size:       5380
 
SalI G'TCGA_C

Cuts at:   1936    2722    1936 
   Size:        786    4594
 
SanDI GG'GwC_CC

Cuts at:   2101    2101 
   Size:       5380
 
SbfI CC_TGCA'GG

Cuts at:   2732    2732 
   Size:       5380
 
ScaI AGT'ACT

Cuts at:   3628    5340    3628 
   Size:       1712    3668
 
SfcI C'TryA_G

Cuts at:    826    1045    2073    2332    2576    2646    2728    3863    4544 
   Size:        219    1028     259     244      70      82    1135     681

Cuts at:   4544    4735     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1135    1028     681     259     244     219     191
                  82      70
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5380
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5380
 
SmaI CCC'GGG

Cuts at:   1872    2707    1872 
   Size:        835    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2618    3484    4385    4647     820 
   Size:        197     926     675     866     901     262    1553

  Fragments arranged by size: 

                1553     926     901     866     675     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3171     493 
   Size:       2678    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5380
 
SphI G_CATG'C

Cuts at:   1132    2738    1132 
   Size:       1606    3774
 
SspI AAT'ATT

Cuts at:      5      52    3304       5 
   Size:         47    3252    2081

  Fragments arranged by size: 

                3252    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2330    1517 
   Size:        813    4567
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2601    2819     513 
   Size:        552     939     597     218    3074

  Fragments arranged by size: 

                3074     939     597     552     218
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3407    3424    3577    3762    4207     325 
   Size:       1255    1827      17     153     185     445    1498

  Fragments arranged by size: 

                1827    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3626    5338      96 
   Size:        274      80      33      51    1443    1649    1712     138

  Fragments arranged by size: 

                1712    1649    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2497    2529    5340    5372    2497 
   Size:         32    2811      32    2505

  Fragments arranged by size: 

                2811    2505      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5380
 
XbaI T'CTAG_A

Cuts at:   2716    2716 
   Size:       5380
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2272    2489    2272 
   Size:        217    5163
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5380
 
XmaI C'CCGG_G

Cuts at:   1870    2705    1870 
   Size:        835    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3509    3509 
   Size:       5380
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3188     276 
   Size:         53      83     186     936    1654    2468

  Fragments arranged by size: 

                2468    1654     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII
     SalI    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PspXI     PsrI    RsrII     SacI    SacII     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
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