Restriction map for pFN21AE0908
 (Linear) (Six-base) MAPSORT of: KIEE0908.seq  Check: 3786  from: 1  to: 675



KIEE0908.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     675 
   Size:        583      92
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      83     675 
   Size:         83     592
 
AlwNI CAG_nnn'CTG

Cuts at:      0     106     426     483     640     675 
   Size:        106     320      57     157      35

  Fragments arranged by size: 

                 320     157     106      57      35
 
ApaI G_GGCC'C

Cuts at:      0     199     675 
   Size:        199     476
 
ApaLI G'TGCA_C

Cuts at:      0      45     675 
   Size:         45     630
 
BanI G'GyrC_C

Cuts at:      0      59     675 
   Size:         59     616
 
BanII G_rGCy'C

Cuts at:      0     199     666     675 
   Size:        199     467       9

  Fragments arranged by size: 

                 467     199       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       2      36     675 
   Size:          2      34     639

  Fragments arranged by size: 

                 639      34       2
 
BglI GCCn_nnn'nGGC

Cuts at:      0     422     431     675 
   Size:        422       9     244

  Fragments arranged by size: 

                 422     244       9
 
BlpI GC'TnA_GC

Cuts at:      0     380     675 
   Size:        380     295
 
Bme1580I G_kGCm'C

Cuts at:      0      49     199     675 
   Size:         49     150     476

  Fragments arranged by size: 

                 476     150      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     403     520     617     675 
   Size:        169     234     117      97      58

  Fragments arranged by size: 

                 234     169     117      97      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     675 
   Size:        598      77
 
BsaHI Gr'CG_yC

Cuts at:      0     446     675 
   Size:        446     229
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     492     522     675 
   Size:        492      30     153

  Fragments arranged by size: 

                 492     153      30
 
BseYI C'CCAG_C

Cuts at:      0     172     429     476     675 
   Size:        172     257      47     199

  Fragments arranged by size: 

                 257     199     172      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     393     637     675 
   Size:        393     244      38

  Fragments arranged by size: 

                 393     244      38
 
BsiHKAI G_wGCw'C

Cuts at:      0      49      56     155     675 
   Size:         49       7      99     520

  Fragments arranged by size: 

                 520      99      49       7
 
Bsp1286I G_dGCh'C

Cuts at:      0      49      56     155     199     666     675 
   Size:         49       7      99      44     467       9

  Fragments arranged by size: 

                 467      99      49      44       9       7
 
BsrDI GCAATG_nn'

Cuts at:      0     437     675 
   Size:        437     238
 
BsrFI r'CCGG_y

Cuts at:      0     608     675 
   Size:        608      67
 
BssSI C'ACGA_G

Cuts at:      0      21     675 
   Size:         21     654
 
BtsI GCAGTG_nn'

Cuts at:      0      77     374     469     675 
   Size:         77     297      95     206

  Fragments arranged by size: 

                 297     206      95      77
 
EaeI y'GGCC_r

Cuts at:      0     593     675 
   Size:        593      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     403     520     583     617     675 
   Size:        169     234     117      63      34      58

  Fragments arranged by size: 

                 234     169     117      63      58      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0      96     103     176     196     423     675 
   Size:         96       7      73      20     227     252

  Fragments arranged by size: 

                 252     227      96      73      20       7
 
HaeII r_GCGC'y

Cuts at:      0     591     675 
   Size:        591      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     615     647     675 
   Size:        615      32      28

  Fragments arranged by size: 

                 615      32      28
 
HincII GTy'rAC

Cuts at:      0     267     675 
   Size:        267     408
 
MspA1I CmG'CkG

Cuts at:      0     483     640     675 
   Size:        483     157      35

  Fragments arranged by size: 

                 483     157      35
 
PasI CC'CwG_GG

Cuts at:      0     100     254     675 
   Size:        100     154     421

  Fragments arranged by size: 

                 421     154     100
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     106     543     675 
   Size:        106     437     132

  Fragments arranged by size: 

                 437     132     106
 
PpuMI rG'GwC_Cy

Cuts at:      0      96     176     675 
   Size:         96      80     499

  Fragments arranged by size: 

                 499      96      80
 
PspOMI G'GGCC_C

Cuts at:      0     195     675 
   Size:        195     480
 
PstI C_TGCA'G

Cuts at:      0      72     575     645     675 
   Size:         72     503      70      30

  Fragments arranged by size: 

                 503      72      70      30
 
PvuII CAG'CTG

Cuts at:      0     640     675 
   Size:        640      35
 
SanDI GG'GwC_CC

Cuts at:      0      96     675 
   Size:         96     579
 
SfcI C'TryA_G

Cuts at:      0      68     327     571     641     675 
   Size:         68     259     244      70      34

  Fragments arranged by size: 

                 259     244      70      68      34
 
SmlI C'TyrA_G

Cuts at:      0     613     675 
   Size:        613      62
 
StuI AGG'CCT

Cuts at:      0     325     675 
   Size:        325     350
 
StyI C'CwwG_G

Cuts at:      0     596     675 
   Size:        596      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     492     524     675 
   Size:        492      32     151

  Fragments arranged by size: 

                 492     151      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     267     484     675 
   Size:        267     217     191

  Fragments arranged by size: 

                 267     217     191
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    ApaLI     BanI    BanII     BcgI
     BglI     BlpI Bme1580I     BpmI    BpuEI    BsaHI    BsaXI    BseYI
     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrFI    BssSI     BtsI     EaeI
  Eco57MI EcoO109I    HaeII    Hin4I   HincII   MspA1I     PasI    PflMI
    PpuMI   PspOMI     PstI    PvuII    SanDI     SfcI     SmlI     StuI
     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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