Restriction map for pFN21AB6437
 (Circular) (Six-base) MAPSORT of: pFN21AB6437.seq  Check: 5136  from: 1  to: 5644



pFN21AB6437  5644 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:24  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3454     278 
   Size:         53      83     186     936    1918    2468

  Fragments arranged by size: 

                2468    1918     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2346    2987    1937 
   Size:        409     641    4594

  Fragments arranged by size: 

                4594     641     409
 
Acc65I G'GTAC_C

Cuts at:   2965    2965 
   Size:       5644
 
AclI AA'CG_TT

Cuts at:   1563    3771    4144    1563 
   Size:       2208     373    3063

  Fragments arranged by size: 

                3063    2208     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2594    3707    5091    5359    1632 
   Size:        962    1113    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1113     962     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5447
 
AflIII A'CryG_T

Cuts at:   1203    4543    5520    1203 
   Size:       3340     977    1327

  Fragments arranged by size: 

                3340    1327     977
 
AgeI A'CCGG_T

Cuts at:   4475    4475 
   Size:       5644
 
AhdI GACnn_n'nnGTC

Cuts at:   4373    4373 
   Size:       5644
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5644
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5644
 
AlwNI CAG_nnn'CTG

Cuts at:   3387    4959    3387 
   Size:       1572    4072
 
ApaLI G'TGCA_C

Cuts at:   2655    3701    4857    2655 
   Size:       1046    1156    3442

  Fragments arranged by size: 

                3442    1156    1046
 
ApoI r'AATT_y

Cuts at:   1493    2119    2162    2328    2449    2953    3179    3331    5440 
   Size:        626      43     166     121     504     226     152    2109

Cuts at:   5440    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697     626     504     226     166     152     121
                  43
 
AseI AT'TA_AT

Cuts at:    160    1032    4198     160 
   Size:        872    3166    1606

  Fragments arranged by size: 

                3166    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5644
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2969    1870 
   Size:         73    1026    4545

  Fragments arranged by size: 

                4545    1026      73
 
AvrII C'CTAG_G

Cuts at:   2875    2875 
   Size:       5644
 
BamHI G'GATC_C

Cuts at:   2974    3341    2974 
   Size:        367    5277
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2965    4420     618 
   Size:        325     217     627    1178    1455    1842

  Fragments arranged by size: 

                1842    1455    1178     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2038    2963    1391 
   Size:        647     925    4072

  Fragments arranged by size: 

                4072     925     647
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4673
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3835    3869    1903 
   Size:         34    1898      34    3678

  Fragments arranged by size: 

                3678    1898      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2002    3536    4746    2002 
   Size:       1534    1210    2900

  Fragments arranged by size: 

                2900    1534    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5644
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2079    4255     136 
   Size:        107     122      71     631     605     407    2176    1525

  Fragments arranged by size: 

                2176    1525     631     605     407     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5639    5639 
   Size:       5644
 
BlpI GC'TnA_GC

Cuts at:   3061    3061 
   Size:       5644
 
Bme1580I G_kGCm'C

Cuts at:   1790    2659    3705    4861    1790 
   Size:        869    1046    1156    2573

  Fragments arranged by size: 

                2573    1156    1046     869
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5644
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3375    4333     442 
   Size:        416      53      83     399      24    1958     958    1753

  Fragments arranged by size: 

                1958    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5644
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2507    2507 
   Size:       5644
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2288    4304    1239 
   Size:       1049    2016    2579

  Fragments arranged by size: 

                2579    2016    1049
 
Bpu10I CC'TnA_GC

Cuts at:   2139    2478    2139 
   Size:        339    5305
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3769    4634    4932    3769 
   Size:        865     298    4481

  Fragments arranged by size: 

                4481     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2431    4307     882 
   Size:        617     932    1876    2219

  Fragments arranged by size: 

                2219    1876     932     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3435     493 
   Size:        711    2231    2702

  Fragments arranged by size: 

                2702    2231     711
 
BsaBI GATnn'nnATC

Cuts at:   5638    5638 
   Size:       5644
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3451    3833    4468     275 
   Size:         53      83     186     936    1918     382     635    1451

  Fragments arranged by size: 

                1918    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4077    4475    4749    4896    5307    4077 
   Size:        398     274     147     411    4414

  Fragments arranged by size: 

                4414     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2709    2739    1178 
   Size:         30     165      30    1306      30    4083

  Fragments arranged by size: 

                4083    1306     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2547    1186 
   Size:       1361    4283
 
BseYI C'CCAG_C

Cuts at:   2037    2060    4847    5343    2037 
   Size:         23    2787     496    2338

  Fragments arranged by size: 

                2787    2338     496      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5071
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3094    3855    4883    1540 
   Size:        275     186    1093     761    1028    2301

  Fragments arranged by size: 

                2301    1093    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2659    3705    3790    4861    2659 
   Size:       1046      85    1071    3442

  Fragments arranged by size: 

                3442    1071    1046      85
 
BsmI GAATG_Cn'

Cuts at:   2303    3155    3248    2303 
   Size:        852      93    4699

  Fragments arranged by size: 

                4699     852      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2038    2659    2963    3705    3790    4861    1391 
   Size:        399     248     621     304     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     621     399     304     248      85
            
 
BspEI T'CCGG_A

Cuts at:   5307    5307 
   Size:       5644
 
BspHI T'CATG_A

Cuts at:   3533    5263    3533 
   Size:       1730    3914
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2999     844 
   Size:       2155    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3531    1394 
   Size:        429    1708    3507

  Fragments arranged by size: 

                3507    1708     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4139    4313      65 
   Size:       1183    2891     174    1396

  Fragments arranged by size: 

                2891    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4288    4475    1700 
   Size:        110    2478     187    2869

  Fragments arranged by size: 

                2869    2478     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2770      96 
   Size:       2674    2970
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5644
 
BssSI C'ACGA_G

Cuts at:   3704    4716    3704 
   Size:       1012    4632
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5644
 
BstBI TT'CG_AA

Cuts at:   4448    4448 
   Size:       5644
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5644
 
BstYI r'GATC_y

Cuts at:   1972    2860    2974    3341    3726    3743    5195    5281    5293 
   Size:        888     114     367     385      17    1452      86      12

Cuts at:   5293    5639    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     888     385     367     346     114      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2151     513 
   Size:        552     939     147    4006

  Fragments arranged by size: 

                4006     939     552     147
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4905

  Fragments arranged by size: 

                4905     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3160    3954    3974    3160 
   Size:        794      20    4830

  Fragments arranged by size: 

                4830     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5644
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5609
 
DraI TTT'AAA

Cuts at:   2290    2948    2290 
   Size:        658    4986
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4651     809 
   Size:        507    3335    1802

  Fragments arranged by size: 

                3335    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3091    3980    5609 
   Size:         54    1425      50     275      18    1261     889    1629

Cuts at:   5609       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1261     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3091    1537 
   Size:        275    1279    4090

  Fragments arranged by size: 

                4090    1279     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2046    3574    1353 
   Size:        693    1528    3423

  Fragments arranged by size: 

                3423    1528     693
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4231    4605    4751     387 
   Size:       1064     138    2642     374     146    1280

  Fragments arranged by size: 

                2642    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2288    2594    3707    4304    5091    5359    1239 
   Size:        393     656     306    1113     597     787     268    1524

  Fragments arranged by size: 

                1524    1113     787     656     597     393     306     268
            
 
EcoRI G'AATT_C

Cuts at:   2328    2953    3331    2328 
   Size:        625     378    4641

  Fragments arranged by size: 

                4641     625     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2821    2821 
   Size:       5644
 
FspI TGC'GCA

Cuts at:   4150    4150 
   Size:       5644
 
HaeII r_GCGC'y

Cuts at:   1678    4791    1678 
   Size:       3113    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2261    2293    2479    2511    4285 
   Size:         32     135      32     689      32     186      32    1774

Cuts at:   4285    4317    4359    4391    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1774     689     186     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2257    2391    2988    3234     669 
   Size:        988     281     319     134     597     246    3079

  Fragments arranged by size: 

                3079     988     597     319     281     246     134
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5644
 
HpaI GTT'AAC

Cuts at:   2257    3234    2257 
   Size:        977    4667
 
KpnI G_GTAC'C

Cuts at:   2969    2969 
   Size:       5644
 
MfeI C'AATT_G

Cuts at:   2829    3243    2829 
   Size:        414    5230
 
MluI A'CGCG_T

Cuts at:   5520    5520 
   Size:       5644
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2849    4758    4942    1305 
   Size:       1544    1909     184    2007

  Fragments arranged by size: 

                2007    1909    1544     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3822

  Fragments arranged by size: 

                3822    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2156    2538    3602    3961    4120     518 
   Size:        648     990     382    1064     359     159    2042

  Fragments arranged by size: 

                2042    1064     990     648     382     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3060    3737    4885    5130    1606 
   Size:       1454     677    1148     245    2120

  Fragments arranged by size: 

                2120    1454    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2151     513 
   Size:        552     939     147    4006

  Fragments arranged by size: 

                4006     939     552     147
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5644
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5644
 
NotI GC'GGCC_GC

Cuts at:   3091    3091 
   Size:       5644
 
NspI r_CATG'y

Cuts at:   1132    1229    2086    2345    3002    4547    1132 
   Size:         97     857     259     657    1545    2229

  Fragments arranged by size: 

                2229    1545     857     657     259      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5644
 
PciI A'CATG_T

Cuts at:   4543    4543 
   Size:       5644
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5644
 
PfoI T'CCnGG_A

Cuts at:   2323    2323 
   Size:       5644
 
PmeI GTTT'AAAC

Cuts at:   2948    2948 
   Size:       5644
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3713    3745    5226    5258    3713 
   Size:         32    1481      32    4099

  Fragments arranged by size: 

                4099    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5644
 
PsiI TTA'TAA

Cuts at:   3214    3214 
   Size:       5644
 
PstI C_TGCA'G

Cuts at:    830    2575    2996     830 
   Size:       1745     421    3478

  Fragments arranged by size: 

                3478    1745     421
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5644
 
SalI G'TCGA_C

Cuts at:   1936    2986    1936 
   Size:       1050    4594
 
SbfI CC_TGCA'GG

Cuts at:   2996    2996 
   Size:       5644
 
ScaI AGT'ACT

Cuts at:   3892    5604    3892 
   Size:       1712    3932
 
SfcI C'TryA_G

Cuts at:    826    1045    2571    2671    2868    2881    2992    4127    4808 
   Size:        219    1526     100     197      13     111    1135     681

Cuts at:   4808    4999     826 
   Size:        191    1471

  Fragments arranged by size: 

                1526    1471    1135     681     219     197     191     111
                 100      13
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5644
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5644
 
SmaI CCC'GGG

Cuts at:   1872    2971    1872 
   Size:       1099    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3748    4649    4911     820 
   Size:        197     926    1805     901     262    1553

  Fragments arranged by size: 

                1805    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3435     493 
   Size:       2942    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5644
 
SphI G_CATG'C

Cuts at:   1132    2086    3002    1132 
   Size:        954     916    3774

  Fragments arranged by size: 

                3774     954     916
 
SspI AAT'ATT

Cuts at:      5      52    2242    3568       5 
   Size:         47    2190    1326    2081

  Fragments arranged by size: 

                2190    2081    1326      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5644
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2151    2875    3083     513 
   Size:        552     939     147     724     208    3074

  Fragments arranged by size: 

                3074     939     724     552     208     147
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2392    3671    3688    3841    4026    4471     325 
   Size:       1255     812    1279      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1279    1255     812     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2770    3890    5602 
   Size:        274      80      33      51    1443     793    1120    1712

Cuts at:   5602      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1120     793     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2769    2801    5604    5636    2769 
   Size:         32    2803      32    2777

  Fragments arranged by size: 

                2803    2777      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5644
 
XbaI T'CTAG_A

Cuts at:   2980    2980 
   Size:       5644
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5644
 
XmaI C'CCGG_G

Cuts at:   1870    2969    1870 
   Size:       1099    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3773    3773 
   Size:       5644
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3452     276 
   Size:         53      83     186     936    1918    2468

  Fragments arranged by size: 

                2468    1918     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI     BaeI     BbeI    BbvCI
    BfrBI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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