################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AocI ApoI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsiEI BsiWI BsmI BspLU11I BsrBI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KpnI MaeII MamI MluI MluNI MscI MseI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 584 TCCGGA T^CCGGA AcyI 3 GGCGCC G[GA]^CG[CT]C AgeI 437 ACCGGT A^CCGGT AhdI 910 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 441 GTGCAC G^TGCAC Aor51HI 159 AGCGCT AGC^GCT ApaI 200 GGGCCC GGGCC^C ApaLI 441 GTGCAC G^TGCAC AscI 212 GGCGCGCC GG^CGCGCC AspEI 910 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 441 GTGCAC G[AT]GC[AT]^C BbeI 3 GGCGCC GGCGC^C BbvCI 871 GCTGAGG CCTCAGC(-5/-2) BclI 342 TGATCA T^GATCA BlpI 317 GCTCAGC GC^T[ACGT]AGC Bpu1102I 317 GCTCAGC GC^T[ACGT]AGC BsaHI 3 GGCGCC G[GA]^CG[CT]C BseAI 584 TCCGGA T^CCGGA BsiHKAI 441 GTGCAC G[AT]GC[AT]^C BsmBI 927 CGTCTC CGTCTC(1/5) BspDI 1180 ATCGAT AT^CGAT BspEI 584 TCCGGA T^CCGGA BspMI 303 GCAGGT ACCTGC(4/8) BssHII 213 GCGCGC G^CGCGC BssSI 140 CACGAG CACGAG(-5/-1) BstYI 403 GGATCT [GA]^GATC[CT] CelII 317 GCTCAGC GC^T[ACGT]AGC ClaI 1180 ATCGAT AT^CGAT EaeI 92 CGGCCA [CT]^GGCC[GA] Eam1105I 910 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 159 AGCGCT AGC^GCT FbaI 342 TGATCA T^GATCA Hin1I 3 GGCGCC G[GA]^CG[CT]C HincII 1246 GTCAAC GT[CT]^[GA]AC HindII 1246 GTCAAC GT[CT]^[GA]AC HphI 723 GGTGA GGTGA(8/7) KasI 3 GGCGCC G^GCGCC MfeI 350 CAATTG C^AATTG MflI 403 GGATCT [GA]^GATC[CT] MroI 584 TCCGGA T^CCGGA MunI 350 CAATTG C^AATTG NarI 3 GGCGCC GG^CGCC NcoI 173 CCATGG C^CATGG PflMI 1060 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 437 ACCGGT A^CCGGT PshAI 969 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 301 CTGCAG CTGCA^G SapI 116 GCTCTTC GCTCTTC(1/4) SfcI 301 CTGCAG C^T[GA][CT]AG SfoI 3 GGCGCC GGC^GCC Van91I 1060 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 403 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 1272 GATCC GGATC(4/5) 403 GGATC BanI 3 GGCGCC G^G[CT][GA]CC 629 GGTGCC BanII 114 GGGCTC G[GA]GC[CT]^C 200 GGGCCC BpmI 425 CTGGAG CTGGAG(16/14) 669 CTGGAG BsaI 850 GGTCTC GGTCTC(1/5) 1034 GGTCTC BsaWI 437 ACCGGT [AT]^CCGG[AT] 584 TCCGGA BsgI 537 CTGCAC GTGCAG(16/14) 618 GTGCAG Bsp1407I 499 TGTACA T^GTACA 981 TGTACA BspHI 339 TCATGA T^CATGA 1148 TCATGA BsrGI 499 TGTACA T^GTACA 981 TGTACA BsrI 1255 ACTGG ACTGG(1/-1) 191 CCAGT BstAPI 623 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1063 GCAAATGGTGC BstXI 635 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 815 CCAAGTGCATGG EcoNI 297 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1292 CCTTGGAGAGG KspI 89 CCGCGG CCGC^GG 122 CCGCGG NaeI 231 GCCGGC GCC^GGC 957 GCCGGC NgoMIV 231 GCCGGC G^CCGGC 957 GCCGGC NspI 559 GCATGT [GA]CATG^[CT] 1314 ACATGC PvuII 320 CAGCTG CAG^CTG 1156 CAGCTG RcaI 339 TCATGA T^CATGA 1148 TCATGA SacII 89 CCGCGG CCGC^GG 122 CCGCGG SspBI 499 TGTACA T^GTACA 981 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AvaI AvaII BbvI BcnI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI