(Linear) (Six-base) MAPSORT of: KIEE1409.seq Check: 9292 from: 1 to: 1404 KIEE1409.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1049 1404 Size: 1049 355 AfeI AGC'GCT Cuts at: 0 161 1404 Size: 161 1243 AgeI A'CCGG_T Cuts at: 0 437 1404 Size: 437 967 AhdI GACnn_n'nnGTC Cuts at: 0 915 1404 Size: 915 489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 582 1404 Size: 582 822 AlwNI CAG_nnn'CTG Cuts at: 0 1008 1027 1387 1404 Size: 1008 19 360 17 Fragments arranged by size: 1008 360 19 17 ApaI G_GGCC'C Cuts at: 0 204 1404 Size: 204 1200 ApaLI G'TGCA_C Cuts at: 0 441 1404 Size: 441 963 AscI GG'CGCG_CC Cuts at: 0 213 1404 Size: 213 1191 AvaI C'yCGr_G Cuts at: 0 58 197 203 1404 Size: 58 139 6 1201 Fragments arranged by size: 1201 139 58 6 BanI G'GyrC_C Cuts at: 0 3 629 1404 Size: 3 626 775 Fragments arranged by size: 775 626 3 BanII G_rGCy'C Cuts at: 0 118 204 1404 Size: 118 86 1200 Fragments arranged by size: 1200 118 86 BbeI G_GCGC'C Cuts at: 0 7 1404 Size: 7 1397 BbvCI CC'TCA_GC Cuts at: 0 872 1404 Size: 872 532 BclI T'GATC_A Cuts at: 0 342 1404 Size: 342 1062 BlpI GC'TnA_GC Cuts at: 0 318 1404 Size: 318 1086 Bme1580I G_kGCm'C Cuts at: 0 198 204 445 634 1127 1404 Size: 198 6 241 189 493 277 Fragments arranged by size: 493 277 241 198 189 6 BmrI ACTGGGnnnn_n' Cuts at: 0 185 1404 Size: 185 1219 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 178 1404 Size: 178 1226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 690 1404 Size: 446 244 714 Fragments arranged by size: 714 446 244 Bpu10I CC'TnA_GC Cuts at: 0 795 872 1404 Size: 795 77 532 Fragments arranged by size: 795 532 77 BsaI GGTCTCn'nnnn_ Cuts at: 0 856 1040 1404 Size: 856 184 364 Fragments arranged by size: 856 364 184 BsaHI Gr'CG_yC Cuts at: 0 4 1404 Size: 4 1400 BsaWI w'CCGG_w Cuts at: 0 437 584 1404 Size: 437 147 820 Fragments arranged by size: 820 437 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 988 1018 1404 Size: 988 30 386 Fragments arranged by size: 988 386 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 170 1056 1059 1404 Size: 170 886 3 345 Fragments arranged by size: 886 345 170 3 BseYI C'CCAG_C Cuts at: 0 100 1011 1404 Size: 100 911 393 Fragments arranged by size: 911 393 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 522 639 1404 Size: 522 117 765 Fragments arranged by size: 765 522 117 BsiHKAI G_wGCw'C Cuts at: 0 445 1404 Size: 445 959 BsmBI CGTCTCn'nnnn_ Cuts at: 0 933 1404 Size: 933 471 Bsp1286I G_dGCh'C Cuts at: 0 118 198 204 445 634 1127 1404 Size: 118 80 6 241 189 493 277 Fragments arranged by size: 493 277 241 189 118 80 6 BspEI T'CCGG_A Cuts at: 0 584 1404 Size: 584 820 BspHI T'CATG_A Cuts at: 0 339 1148 1404 Size: 339 809 256 Fragments arranged by size: 809 339 256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 294 1404 Size: 294 1110 BsrDI GCAATG_nn' Cuts at: 0 370 711 1235 1404 Size: 370 341 524 169 Fragments arranged by size: 524 370 341 169 BsrFI r'CCGG_y Cuts at: 0 231 437 957 1017 1404 Size: 231 206 520 60 387 Fragments arranged by size: 520 387 231 206 60 BsrGI T'GTAC_A Cuts at: 0 499 981 1404 Size: 499 482 423 Fragments arranged by size: 499 482 423 BssHII G'CGCG_C Cuts at: 0 213 1404 Size: 213 1191 BssSI C'ACGA_G Cuts at: 0 140 1404 Size: 140 1264 BstAPI GCAn_nnn'nTGC Cuts at: 0 629 1069 1404 Size: 629 440 335 Fragments arranged by size: 629 440 335 BstXI CCAn_nnnn'nTGG Cuts at: 0 642 822 1404 Size: 642 180 582 Fragments arranged by size: 642 582 180 BstYI r'GATC_y Cuts at: 0 403 1404 Size: 403 1001 BtgI C'CryG_G Cuts at: 0 89 122 173 1404 Size: 89 33 51 1231 Fragments arranged by size: 1231 89 51 33 BtsI GCAGTG_nn' Cuts at: 0 50 1404 Size: 50 1354 ClaI AT'CG_AT Cuts at: 0 1181 1404 Size: 1181 223 EaeI y'GGCC_r Cuts at: 0 92 1404 Size: 92 1312 EarI CTCTTCn'nnn_ Cuts at: 0 123 518 1301 1404 Size: 123 395 783 103 Fragments arranged by size: 783 395 123 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 690 1049 1404 Size: 446 244 359 355 Fragments arranged by size: 446 359 355 244 EcoNI CCTnn'n_nnAGG Cuts at: 0 301 1296 1404 Size: 301 995 108 Fragments arranged by size: 995 301 108 EcoO109I rG'GnC_Cy Cuts at: 0 223 294 810 862 1335 1404 Size: 223 71 516 52 473 69 Fragments arranged by size: 516 473 223 71 69 52 HaeII r_GCGC'y Cuts at: 0 7 163 846 1404 Size: 7 156 683 558 Fragments arranged by size: 683 558 156 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 39 71 629 661 901 933 1404 Size: 39 32 558 32 240 32 471 Fragments arranged by size: 558 471 240 39 32 32 32 HincII GTy'rAC Cuts at: 0 1248 1404 Size: 1248 156 KasI G'GCGC_C Cuts at: 0 3 1404 Size: 3 1401 MfeI C'AATT_G Cuts at: 0 350 1404 Size: 350 1054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 497 1404 Size: 497 907 MslI CAynn'nnrTG Cuts at: 0 29 363 564 1404 Size: 29 334 201 840 Fragments arranged by size: 840 334 201 29 MspA1I CmG'CkG Cuts at: 0 91 124 322 1158 1404 Size: 91 33 198 836 246 Fragments arranged by size: 836 246 198 91 33 NaeI GCC'GGC Cuts at: 0 233 959 1404 Size: 233 726 445 Fragments arranged by size: 726 445 233 NarI GG'CG_CC Cuts at: 0 4 1404 Size: 4 1400 NcoI C'CATG_G Cuts at: 0 173 1404 Size: 173 1231 NgoMIV G'CCGG_C Cuts at: 0 231 957 1404 Size: 231 726 447 Fragments arranged by size: 726 447 231 NspI r_CATG'y Cuts at: 0 563 1318 1404 Size: 563 755 86 Fragments arranged by size: 755 563 86 PasI CC'CwG_GG Cuts at: 0 991 1210 1404 Size: 991 219 194 Fragments arranged by size: 991 219 194 PflMI CCAn_nnn'nTGG Cuts at: 0 1066 1404 Size: 1066 338 PfoI T'CCnGG_A Cuts at: 0 423 1404 Size: 423 981 PpuMI rG'GwC_Cy Cuts at: 0 223 294 810 1335 1404 Size: 223 71 516 525 69 Fragments arranged by size: 525 516 223 71 69 PshAI GACnn'nnGTC Cuts at: 0 973 1404 Size: 973 431 PspOMI G'GGCC_C Cuts at: 0 200 1404 Size: 200 1204 PstI C_TGCA'G Cuts at: 0 305 1404 Size: 305 1099 PvuII CAG'CTG Cuts at: 0 322 1158 1404 Size: 322 836 246 Fragments arranged by size: 836 322 246 SacII CC_GC'GG Cuts at: 0 92 125 1404 Size: 92 33 1279 Fragments arranged by size: 1279 92 33 SanDI GG'GwC_CC Cuts at: 0 810 1404 Size: 810 594 SapI GCTCTTCn'nnn_ Cuts at: 0 123 1404 Size: 123 1281 SfcI C'TryA_G Cuts at: 0 301 1404 Size: 301 1103 SfoI GGC'GCC Cuts at: 0 5 1404 Size: 5 1399 SmaI CCC'GGG Cuts at: 0 60 199 205 1404 Size: 60 139 6 1199 Fragments arranged by size: 1199 139 60 6 SrfI GCCC'GGGC Cuts at: 0 199 205 1404 Size: 199 6 1199 Fragments arranged by size: 1199 199 6 StyI C'CwwG_G Cuts at: 0 173 1292 1397 1404 Size: 173 1119 105 7 Fragments arranged by size: 1119 173 105 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 114 711 1404 Size: 114 597 693 Fragments arranged by size: 693 597 114 TatI w'GTAC_w Cuts at: 0 21 499 981 1404 Size: 21 478 482 423 Fragments arranged by size: 482 478 423 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 802 817 834 849 988 1020 1404 Size: 802 15 17 15 139 32 384 Fragments arranged by size: 802 384 139 32 17 15 15 XmaI C'CCGG_G Cuts at: 0 58 197 203 1404 Size: 58 139 6 1201 Fragments arranged by size: 1201 139 58 6 Enzymes that do cut: AcuI AfeI AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AvaI BanI BanII BbeI BbvCI BclI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI ClaI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MfeI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacII SanDI SapI SfcI SfoI SmaI SrfI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AleI AloI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BglI BglII BmgBI BmtI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BsrBI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MluI MscI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI