################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BpuAI BsaAI BsaWI BseAI BsiWI BspEI BspHI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 31 GGCGCC G[GA]^CG[CT]C AflIII 1058 ACATGT A^C[GA][CT]GT BanI 31 GGCGCC G^G[CT][GA]CC BbeI 31 GGCGCC GGCGC^C BclI 297 TGATCA T^GATCA BsaBI 1800 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 31 GGCGCC G[GA]^CG[CT]C BsgI 1333 GTGCAG GTGCAG(16/14) BsiEI 5 CGGTCG CG[GA][CT]^CG BsmBI 1045 GAGACG CGTCTC(1/5) Bsp1407I 809 TGTACA T^GTACA BspDI 415 ATCGAT AT^CGAT BspLU11I 1058 ACATGT A^CATGT BsrBI 1168 GAGCGG CCGCTC(-3/-3) BsrFI 461 GCCGGC [GA]^CCGG[CT] BsrGI 809 TGTACA T^GTACA BstXI 1508 CCAACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 461 GCCGGC [GA]^CCGG[CT] ClaI 415 ATCGAT AT^CGAT FbaI 297 TGATCA T^GATCA HaeII 31 GGCGCC [GA]GCGC^[CT] Hin1I 31 GGCGCC G[GA]^CG[CT]C KasI 31 GGCGCC G^GCGCC MamI 1800 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 236 TTAA T^TAA NaeI 461 GCCGGC GCC^GGC NarI 31 GGCGCC GG^CGCC NcoI 1359 CCATGG C^CATGG NgoMIV 461 GCCGGC G^CCGGC PshAI 898 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 288 GAAGAGC GCTCTTC(1/4) ScaI 197 AGTACT AGT^ACT SexAI 173 ACCAGGT A^CC[AT]GGT SfoI 31 GGCGCC GGC^GCC SmaI 1575 CCCGGG CCC^GGG SspBI 809 TGTACA T^GTACA StuI 1500 AGGCCT AGG^CCT Tru9I 236 TTAA T^TAA Tsp45I 1071 GTCAC ^GT[GC]AC XmaCI 1575 CCCGGG C^CCGGG XmaI 1575 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 514 GTCTAC GT^[AC][GT]AC 1441 GTCTAC AccII 9 CGCG CG^CG 18 CGCG AvaI 553 CTCGGG C^[CT]CG[GA]G 1575 CCCGGG BalI 479 TGGCCA TGG^CCA 1129 TGGCCA BglII 48 AGATCT A^GATCT 1229 AGATCT BlpI 468 GCTCAGC GC^T[ACGT]AGC 915 GCTTAGC Bpu1102I 468 GCTCAGC GC^T[ACGT]AGC 915 GCTTAGC BsaI 896 GAGACC GGTCTC(1/5) 1173 GGTCTC BseRI 1191 CTCCTC GAGGAG(10/8) 1781 GAGGAG BsmI 693 GAATGC GAATGC(1/-1) 936 GAATGC BsoBI 553 CTCGGG C^[CT]CG[GA]G 1575 CCCGGG BstUI 9 CGCG CG^CG 18 CGCG CelII 468 GCTCAGC GC^T[ACGT]AGC 915 GCTTAGC DsaI 593 CCGTGG C^C[GA][CT]GG 1359 CCATGG EarI 1609 CTCTTC CTCTTC(1/4) 288 GAAGAG HgaI 996 GACGC GACGC(5/10) 1047 GACGC Ksp632I 1609 CTCTTC CTCTTC(1/4) 288 GAAGAG MaeIII 854 GTTAC ^GT[ACGT]AC 1071 GTCAC MluNI 479 TGGCCA TGG^CCA 1129 TGGCCA MscI 479 TGGCCA TGG^CCA 1129 TGGCCA MslI 621 CATTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1719 CATCAGTGTG MvnI 9 CGCG CG^CG 18 CGCG PvuII 963 CAGCTG CAG^CTG 1702 CAGCTG SacI 799 GAGCTC GAGCT^C 1681 GAGCTC TaqI 416 TCGA T^CGA 983 TCGA TthHB8I 416 TCGA T^CGA 983 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI TseI Tsp509I TspRI Van91I XhoII