(Circular) (Six-base) MAPSORT of: pFN21AE6405.seq Check: 5411 from: 1 to: 6523 pFN21AE6405 6523 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2321 3412 3538 2321 Size: 1091 126 5306 Fragments arranged by size: 5306 1091 126 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4333 278 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2520 3447 3866 1937 Size: 583 927 419 4594 Fragments arranged by size: 4594 927 583 419 Acc65I G'GTAC_C Cuts at: 3844 3844 Size: 6523 AclI AA'CG_TT Cuts at: 1563 4650 5023 1563 Size: 3087 373 3063 Fragments arranged by size: 3087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2312 2759 3092 3458 4586 5970 6238 Size: 476 204 447 333 366 1128 1384 268 Cuts at: 6238 1632 Size: 1917 Fragments arranged by size: 1917 1384 1128 476 447 366 333 268 204 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6326 AflIII A'CryG_T Cuts at: 1203 3063 5422 6399 1203 Size: 1860 2359 977 1327 Fragments arranged by size: 2359 1860 1327 977 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 5252 5252 Size: 6523 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6523 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6523 AlwNI CAG_nnn'CTG Cuts at: 2086 3253 3346 3464 3709 4266 5838 2086 Size: 1167 93 118 245 557 1572 2771 Fragments arranged by size: 2771 1572 1167 557 245 118 93 ApaI G_GGCC'C Cuts at: 2022 2624 3363 2022 Size: 602 739 5182 Fragments arranged by size: 5182 739 602 ApaLI G'TGCA_C Cuts at: 4580 5736 4580 Size: 1156 5367 ApoI r'AATT_y Cuts at: 1493 3832 4058 4210 6319 1493 Size: 2339 226 152 2109 1697 Fragments arranged by size: 2339 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5077 160 Size: 872 4045 1606 Fragments arranged by size: 4045 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 2558 3580 3848 1870 Size: 73 615 1022 268 4545 Fragments arranged by size: 4545 1022 615 268 73 BamHI G'GATC_C Cuts at: 3853 4220 3853 Size: 367 6156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 3844 5299 618 Size: 325 217 627 249 1808 1455 1842 Fragments arranged by size: 1842 1808 1455 627 325 249 217 BanII G_rGCy'C Cuts at: 1391 2022 2624 2808 3335 3363 3690 3842 1391 Size: 631 602 184 527 28 327 152 4072 Fragments arranged by size: 4072 631 602 527 327 184 152 28 BbeI G_GCGC'C Cuts at: 2040 2040 Size: 6523 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5552 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4714 4748 1903 Size: 34 2777 34 3678 Fragments arranged by size: 3678 2777 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4415 5625 4415 Size: 1210 5313 BclI T'GATC_A Cuts at: 1548 2302 1548 Size: 754 5769 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5134 136 Size: 107 122 71 631 605 3462 1525 Fragments arranged by size: 3462 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2053 3234 6518 2053 Size: 1181 3284 2058 Fragments arranged by size: 3284 2058 1181 BlpI GC'TnA_GC Cuts at: 2474 2921 3940 2474 Size: 447 1019 5057 Fragments arranged by size: 5057 1019 447 Bme1580I G_kGCm'C Cuts at: 1790 2022 2624 3278 3363 3664 4584 5740 1790 Size: 232 602 654 85 301 920 1156 2573 Fragments arranged by size: 2573 1156 920 654 602 301 232 85 BmgBI CAC'GTC Cuts at: 1318 2452 1318 Size: 1134 5389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2190 4254 5212 Size: 416 53 83 399 24 773 2064 958 Cuts at: 5212 442 Size: 1753 Fragments arranged by size: 2064 1753 958 773 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3196 3196 Size: 6523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2180 2356 2678 5183 1239 Size: 812 129 176 322 2505 2579 Fragments arranged by size: 2579 2505 812 322 176 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3191 3505 3770 4648 5513 5811 3191 Size: 314 265 878 865 298 3903 Fragments arranged by size: 3903 878 865 314 298 265 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2895 3184 5186 882 Size: 617 1396 289 2002 2219 Fragments arranged by size: 2219 2002 1396 617 289 BsaAI yAC'GTr Cuts at: 493 1204 4314 493 Size: 711 3110 2702 Fragments arranged by size: 3110 2702 711 BsaBI GATnn'nnATC Cuts at: 3809 6517 3809 Size: 2708 3815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 4330 4712 5347 Size: 53 83 186 936 504 2293 382 635 Cuts at: 5347 275 Size: 1451 Fragments arranged by size: 2293 1451 936 635 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4956 5354 5628 5775 6186 4956 Size: 398 274 147 411 5293 Fragments arranged by size: 5293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2780 2810 1178 Size: 30 165 30 1377 30 4891 Fragments arranged by size: 4891 1377 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3187 3801 1186 Size: 2001 614 3908 Fragments arranged by size: 3908 2001 614 BseYI C'CCAG_C Cuts at: 2726 2979 3709 5726 6222 2726 Size: 253 730 2017 496 3027 Fragments arranged by size: 3027 2017 730 496 253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3359 1163 Size: 573 1623 4327 Fragments arranged by size: 4327 1623 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3973 4734 5762 1540 Size: 275 186 12 1960 761 1028 2301 Fragments arranged by size: 2301 1960 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2358 2742 2808 3690 4584 4669 5740 2358 Size: 384 66 882 894 85 1071 3141 Fragments arranged by size: 3141 1071 894 882 384 85 66 BsmI GAATG_Cn' Cuts at: 2704 2947 4034 4127 2704 Size: 243 1087 93 5100 Fragments arranged by size: 5100 1087 243 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3044 3044 Size: 6523 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2358 2624 2742 2808 3278 3335 Size: 399 232 336 266 118 66 470 57 Cuts at: 3335 3363 3664 3690 3842 4584 4669 5740 1391 Size: 28 301 26 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 470 399 336 301 266 232 152 118 85 66 57 28 26 BspEI T'CCGG_A Cuts at: 6186 6186 Size: 6523 BspHI T'CATG_A Cuts at: 4412 6142 4412 Size: 1730 4793 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2321 2328 3412 3538 3630 3878 844 Size: 1477 7 1084 126 92 248 3489 Fragments arranged by size: 3489 1477 1084 248 126 92 7 BsrBI CCG'CTC Cuts at: 1394 1823 3175 4410 1394 Size: 429 1352 1235 3507 Fragments arranged by size: 3507 1352 1235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5018 5192 65 Size: 1183 3770 174 1396 Fragments arranged by size: 3770 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2466 5167 5354 1700 Size: 110 656 2701 187 2869 Fragments arranged by size: 2869 2701 656 187 110 BsrGI T'GTAC_A Cuts at: 96 2814 96 Size: 2718 3805 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6523 BssSI C'ACGA_G Cuts at: 4583 5595 4583 Size: 1012 5511 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6523 BstBI TT'CG_AA Cuts at: 5327 5327 Size: 6523 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6523 BstXI CCAn_nnnn'nTGG Cuts at: 3520 3520 Size: 6523 BstYI r'GATC_y Cuts at: 1972 2053 3090 3220 3234 3480 3853 4220 4605 Size: 81 1037 130 14 246 373 367 385 Cuts at: 4605 4622 6074 6160 6172 6518 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1037 385 373 367 346 246 130 86 81 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2598 3364 513 Size: 552 939 594 766 3672 Fragments arranged by size: 3672 939 766 594 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2854 487 Size: 683 56 1628 4156 Fragments arranged by size: 4156 1628 683 56 BtsI GCAGTG_nn' Cuts at: 3645 4039 4833 4853 3645 Size: 394 794 20 5315 Fragments arranged by size: 5315 794 394 20 ClaI AT'CG_AT Cuts at: 1554 2421 1554 Size: 867 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6488 DraI TTT'AAA Cuts at: 3827 3827 Size: 6523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5530 809 Size: 507 4214 1802 Fragments arranged by size: 4214 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2484 3134 3700 Size: 54 1425 50 275 18 654 650 566 Cuts at: 3700 3970 4859 6488 8 Size: 270 889 1629 43 Fragments arranged by size: 1629 1425 889 654 650 566 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3970 1537 Size: 275 2158 4090 Fragments arranged by size: 4090 2158 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2288 3620 4453 1353 Size: 935 1332 833 3423 Fragments arranged by size: 3423 1332 935 833 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5110 5484 5630 387 Size: 1064 138 3521 374 146 1280 Fragments arranged by size: 3521 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2806 3688 2806 Size: 882 5641 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2108 2180 2312 2356 2678 2759 Size: 393 419 57 72 132 44 322 81 Cuts at: 2759 3092 3458 4586 5183 5970 6238 1239 Size: 333 366 1128 597 787 268 1524 Fragments arranged by size: 1524 1128 787 597 419 393 366 333 322 268 132 81 72 57 44 EcoNI CCTnn'n_nnAGG Cuts at: 3215 3440 3668 3215 Size: 225 228 6070 Fragments arranged by size: 6070 228 225 EcoO109I rG'GnC_Cy Cuts at: 2018 2212 2344 2621 3360 3408 3535 2018 Size: 194 132 277 739 48 127 5006 Fragments arranged by size: 5006 739 277 194 132 127 48 EcoRI G'AATT_C Cuts at: 3832 4210 3832 Size: 378 6145 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2962 3130 3307 3412 2962 Size: 168 177 105 6073 Fragments arranged by size: 6073 177 168 105 FspI TGC'GCA Cuts at: 5029 5029 Size: 6523 HaeII r_GCGC'y Cuts at: 1678 2040 5670 1678 Size: 362 3630 2531 Fragments arranged by size: 3630 2531 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2182 2214 2287 2319 2780 Size: 32 135 32 610 32 73 32 461 Cuts at: 2780 2812 3560 3592 5164 5196 5238 5270 1373 Size: 32 748 32 1572 32 42 32 2626 Fragments arranged by size: 2626 1572 748 610 461 135 73 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3867 4113 669 Size: 988 281 1929 246 3079 Fragments arranged by size: 3079 1929 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6523 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KasI G'GCGC_C Cuts at: 2036 2036 Size: 6523 KpnI G_GTAC'C Cuts at: 3848 3848 Size: 6523 MfeI C'AATT_G Cuts at: 4122 4122 Size: 6523 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3537 5637 5821 1305 Size: 2232 2100 184 2007 Fragments arranged by size: 2232 2100 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2486 3136 10 Size: 54 1425 343 654 650 3397 Fragments arranged by size: 3397 1425 654 650 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 3728 4481 4840 4999 518 Size: 648 1464 1098 753 359 159 2042 Fragments arranged by size: 2042 1464 1098 753 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2889 2970 3140 3709 3939 4616 5764 Size: 465 818 81 170 569 230 677 1148 Cuts at: 5764 6009 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 818 677 569 465 245 230 170 81 NaeI GCC'GGC Cuts at: 2468 2468 Size: 6523 NarI GG'CG_CC Cuts at: 2037 2037 Size: 6523 NcoI C'CATG_G Cuts at: 513 1065 2004 3364 513 Size: 552 939 1360 3672 Fragments arranged by size: 3672 1360 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6523 NgoMIV G'CCGG_C Cuts at: 2466 2466 Size: 6523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 2686 2735 3067 3881 5426 1132 Size: 97 1457 49 332 814 1545 2229 Fragments arranged by size: 2229 1545 1457 814 332 97 49 PasI CC'CwG_GG Cuts at: 1348 3128 1348 Size: 1780 4743 PciI A'CATG_T Cuts at: 3063 5422 3063 Size: 2359 4164 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 3550 3709 1108 Size: 1344 1098 159 3922 Fragments arranged by size: 3922 1344 1098 159 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4592 4624 6105 6137 4592 Size: 32 1481 32 4978 Fragments arranged by size: 4978 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2344 3408 2212 Size: 132 1064 5327 Fragments arranged by size: 5327 1064 132 PshAI GACnn'nnGTC Cuts at: 1613 2907 1613 Size: 1294 5229 PsiI TTA'TAA Cuts at: 4093 4093 Size: 6523 PspOMI G'GGCC_C Cuts at: 2018 2620 3359 2018 Size: 602 739 5182 Fragments arranged by size: 5182 739 602 PstI C_TGCA'G Cuts at: 830 2082 2766 3627 3875 830 Size: 1252 684 861 248 3478 Fragments arranged by size: 3478 1252 861 684 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 PvuII CAG'CTG Cuts at: 2970 3709 2970 Size: 739 5784 SacI G_AGCT'C Cuts at: 2808 3690 2808 Size: 882 5641 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SanDI GG'GwC_CC Cuts at: 3408 3408 Size: 6523 SapI GCTCTTCn'nnn_ Cuts at: 2288 2288 Size: 6523 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 2204 4771 6483 2204 Size: 2567 1712 2244 Fragments arranged by size: 2567 2244 1712 SexAI A'CCwGG_T Cuts at: 2178 2178 Size: 6523 SfcI C'TryA_G Cuts at: 826 1045 2078 2719 2762 3623 3871 5006 5687 Size: 219 1033 641 43 861 248 1135 681 Cuts at: 5687 5878 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1033 861 681 641 248 219 191 43 SfoI GGC'GCC Cuts at: 2038 2038 Size: 6523 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 3582 3850 1872 Size: 1710 268 4545 Fragments arranged by size: 4545 1710 268 SmlI C'TyrA_G Cuts at: 820 1017 1943 3170 3484 3749 4627 5528 5790 Size: 197 926 1227 314 265 878 901 262 Cuts at: 5790 820 Size: 1553 Fragments arranged by size: 1553 1227 926 901 878 314 265 262 197 SnaBI TAC'GTA Cuts at: 493 4314 493 Size: 3821 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6523 SphI G_CATG'C Cuts at: 1132 3881 1132 Size: 2749 3774 SspI AAT'ATT Cuts at: 5 52 4447 5 Size: 47 4395 2081 Fragments arranged by size: 4395 2081 47 StuI AGG'CCT Cuts at: 1517 3507 1517 Size: 1990 4533 StyI C'CwwG_G Cuts at: 513 1065 2004 2799 3364 3765 3962 513 Size: 552 939 795 565 401 197 3074 Fragments arranged by size: 3074 939 795 565 552 401 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2993 3296 4550 4567 4720 4905 5350 Size: 1255 1413 303 1254 17 153 185 445 Cuts at: 5350 325 Size: 1498 Fragments arranged by size: 1498 1413 1255 1254 445 303 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 2814 3323 Size: 274 80 33 51 1443 225 612 509 Cuts at: 3323 4769 6481 96 Size: 1446 1712 138 Fragments arranged by size: 1712 1446 1443 612 509 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6483 6515 6483 Size: 32 6491 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6523 XbaI T'CTAG_A Cuts at: 3859 3859 Size: 6523 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6523 XmaI C'CCGG_G Cuts at: 1870 3580 3848 1870 Size: 1710 268 4545 Fragments arranged by size: 4545 1710 268 XmnI GAAnn'nnTTC Cuts at: 4652 4652 Size: 6523 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4331 276 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SfiI SrfI SwaI XcmI