(Linear) (Six-base) MAPSORT of: KIEE6405.seq Check: 7150 from: 1 to: 1818 KIEE6405.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 316 1407 1533 1818 Size: 316 1091 126 285 Fragments arranged by size: 1091 316 285 126 AccI GT'mk_AC Cuts at: 0 515 1442 1818 Size: 515 927 376 Fragments arranged by size: 927 515 376 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 307 754 1087 1453 1818 Size: 103 204 447 333 366 365 Fragments arranged by size: 447 366 365 333 204 103 AflIII A'CryG_T Cuts at: 0 1058 1818 Size: 1058 760 AlwNI CAG_nnn'CTG Cuts at: 0 81 1248 1341 1459 1704 1818 Size: 81 1167 93 118 245 114 Fragments arranged by size: 1167 245 118 114 93 81 ApaI G_GGCC'C Cuts at: 0 17 619 1358 1818 Size: 17 602 739 460 Fragments arranged by size: 739 602 460 17 AvaI C'yCGr_G Cuts at: 0 553 1575 1818 Size: 553 1022 243 Fragments arranged by size: 1022 553 243 BanI G'GyrC_C Cuts at: 0 31 1818 Size: 31 1787 BanII G_rGCy'C Cuts at: 0 17 619 803 1330 1358 1685 1818 Size: 17 602 184 527 28 327 133 Fragments arranged by size: 602 527 327 184 133 28 17 BbeI G_GCGC'C Cuts at: 0 35 1818 Size: 35 1783 BclI T'GATC_A Cuts at: 0 297 1818 Size: 297 1521 BglII A'GATC_T Cuts at: 0 48 1229 1818 Size: 48 1181 589 Fragments arranged by size: 1181 589 48 BlpI GC'TnA_GC Cuts at: 0 469 916 1818 Size: 469 447 902 Fragments arranged by size: 902 469 447 Bme1580I G_kGCm'C Cuts at: 0 17 619 1273 1358 1659 1818 Size: 17 602 654 85 301 159 Fragments arranged by size: 654 602 301 159 85 17 BmgBI CAC'GTC Cuts at: 0 447 1818 Size: 447 1371 BmrI ACTGGGnnnn_n' Cuts at: 0 185 1818 Size: 185 1633 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1191 1818 Size: 1191 627 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 175 351 673 1818 Size: 46 129 176 322 1145 Fragments arranged by size: 1145 322 176 129 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1186 1500 1765 1818 Size: 1186 314 265 53 Fragments arranged by size: 1186 314 265 53 BsaI GGTCTCn'nnnn_ Cuts at: 0 890 1179 1818 Size: 890 289 639 Fragments arranged by size: 890 639 289 BsaBI GATnn'nnATC Cuts at: 0 1804 1818 Size: 1804 14 BsaHI Gr'CG_yC Cuts at: 0 32 1818 Size: 32 1786 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 775 805 1818 Size: 775 30 1013 Fragments arranged by size: 1013 775 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1182 1796 1818 Size: 1182 614 22 Fragments arranged by size: 1182 614 22 BseYI C'CCAG_C Cuts at: 0 721 974 1704 1818 Size: 721 253 730 114 Fragments arranged by size: 730 721 253 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1354 1818 Size: 1354 464 BsiEI CG_ry'CG Cuts at: 0 8 1818 Size: 8 1810 BsiHKAI G_wGCw'C Cuts at: 0 353 737 803 1685 1818 Size: 353 384 66 882 133 Fragments arranged by size: 882 384 353 133 66 BsmI GAATG_Cn' Cuts at: 0 699 942 1818 Size: 699 243 876 Fragments arranged by size: 876 699 243 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1039 1818 Size: 1039 779 Bsp1286I G_dGCh'C Cuts at: 0 17 353 619 737 803 1273 1330 1358 Size: 17 336 266 118 66 470 57 28 Cuts at: 1358 1659 1685 1818 Size: 301 26 133 Fragments arranged by size: 470 336 301 266 133 118 66 57 28 26 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 316 323 1407 1533 1625 1818 Size: 316 7 1084 126 92 193 Fragments arranged by size: 1084 316 193 126 92 7 BsrBI CCG'CTC Cuts at: 0 1170 1818 Size: 1170 648 BsrFI r'CCGG_y Cuts at: 0 461 1818 Size: 461 1357 BsrGI T'GTAC_A Cuts at: 0 809 1818 Size: 809 1009 BstXI CCAn_nnnn'nTGG Cuts at: 0 1515 1818 Size: 1515 303 BstYI r'GATC_y Cuts at: 0 48 1085 1215 1229 1475 1818 Size: 48 1037 130 14 246 343 Fragments arranged by size: 1037 343 246 130 48 14 BtgI C'CryG_G Cuts at: 0 593 1359 1818 Size: 593 766 459 Fragments arranged by size: 766 593 459 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 849 1818 Size: 849 969 BtsI GCAGTG_nn' Cuts at: 0 1640 1818 Size: 1640 178 ClaI AT'CG_AT Cuts at: 0 416 1818 Size: 416 1402 EaeI y'GGCC_r Cuts at: 0 479 1129 1695 1818 Size: 479 650 566 123 Fragments arranged by size: 650 566 479 123 EarI CTCTTCn'nnn_ Cuts at: 0 283 1615 1818 Size: 283 1332 203 Fragments arranged by size: 1332 283 203 EcoICRI GAG'CTC Cuts at: 0 801 1683 1818 Size: 801 882 135 Fragments arranged by size: 882 801 135 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 103 175 307 351 673 754 1087 Size: 46 57 72 132 44 322 81 333 Cuts at: 1087 1453 1818 Size: 366 365 Fragments arranged by size: 366 365 333 322 132 81 72 57 46 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 1210 1435 1663 1818 Size: 1210 225 228 155 Fragments arranged by size: 1210 228 225 155 EcoO109I rG'GnC_Cy Cuts at: 0 13 207 339 616 1355 1403 1530 1818 Size: 13 194 132 277 739 48 127 288 Fragments arranged by size: 739 288 277 194 132 127 48 13 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 957 1125 1302 1407 1818 Size: 957 168 177 105 411 Fragments arranged by size: 957 411 177 168 105 HaeII r_GCGC'y Cuts at: 0 35 1818 Size: 35 1783 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 177 209 282 314 775 807 1555 1587 Size: 177 32 73 32 461 32 748 32 Cuts at: 1587 1818 Size: 231 Fragments arranged by size: 748 461 231 177 73 32 32 32 32 KasI G'GCGC_C Cuts at: 0 31 1818 Size: 31 1787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1532 1818 Size: 1532 286 MscI TGG'CCA Cuts at: 0 481 1131 1818 Size: 481 650 687 Fragments arranged by size: 687 650 481 MslI CAynn'nnrTG Cuts at: 0 625 1723 1818 Size: 625 1098 95 Fragments arranged by size: 1098 625 95 MspA1I CmG'CkG Cuts at: 0 66 884 965 1135 1704 1818 Size: 66 818 81 170 569 114 Fragments arranged by size: 818 569 170 114 81 66 NaeI GCC'GGC Cuts at: 0 463 1818 Size: 463 1355 NarI GG'CG_CC Cuts at: 0 32 1818 Size: 32 1786 NcoI C'CATG_G Cuts at: 0 1359 1818 Size: 1359 459 NgoMIV G'CCGG_C Cuts at: 0 461 1818 Size: 461 1357 NspI r_CATG'y Cuts at: 0 681 730 1062 1818 Size: 681 49 332 756 Fragments arranged by size: 756 681 332 49 PasI CC'CwG_GG Cuts at: 0 1123 1818 Size: 1123 695 PciI A'CATG_T Cuts at: 0 1058 1818 Size: 1058 760 PflMI CCAn_nnn'nTGG Cuts at: 0 447 1545 1704 1818 Size: 447 1098 159 114 Fragments arranged by size: 1098 447 159 114 PpuMI rG'GwC_Cy Cuts at: 0 207 339 1403 1818 Size: 207 132 1064 415 Fragments arranged by size: 1064 415 207 132 PshAI GACnn'nnGTC Cuts at: 0 902 1818 Size: 902 916 PspOMI G'GGCC_C Cuts at: 0 13 615 1354 1818 Size: 13 602 739 464 Fragments arranged by size: 739 602 464 13 PstI C_TGCA'G Cuts at: 0 77 761 1622 1818 Size: 77 684 861 196 Fragments arranged by size: 861 684 196 77 PvuII CAG'CTG Cuts at: 0 965 1704 1818 Size: 965 739 114 Fragments arranged by size: 965 739 114 SacI G_AGCT'C Cuts at: 0 803 1685 1818 Size: 803 882 133 Fragments arranged by size: 882 803 133 SanDI GG'GwC_CC Cuts at: 0 1403 1818 Size: 1403 415 SapI GCTCTTCn'nnn_ Cuts at: 0 283 1818 Size: 283 1535 ScaI AGT'ACT Cuts at: 0 199 1818 Size: 199 1619 SexAI A'CCwGG_T Cuts at: 0 173 1818 Size: 173 1645 SfcI C'TryA_G Cuts at: 0 73 714 757 1618 1818 Size: 73 641 43 861 200 Fragments arranged by size: 861 641 200 73 43 SfoI GGC'GCC Cuts at: 0 33 1818 Size: 33 1785 SmaI CCC'GGG Cuts at: 0 1577 1818 Size: 1577 241 SmlI C'TyrA_G Cuts at: 0 1165 1479 1744 1818 Size: 1165 314 265 74 Fragments arranged by size: 1165 314 265 74 StuI AGG'CCT Cuts at: 0 1502 1818 Size: 1502 316 StyI C'CwwG_G Cuts at: 0 794 1359 1760 1818 Size: 794 565 401 58 Fragments arranged by size: 794 565 401 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 988 1291 1818 Size: 988 303 527 Fragments arranged by size: 988 527 303 TatI w'GTAC_w Cuts at: 0 197 809 1318 1818 Size: 197 612 509 500 Fragments arranged by size: 612 509 500 197 XmaI C'CCGG_G Cuts at: 0 1575 1818 Size: 1575 243 Enzymes that do cut: AarI AccI AcuI AflIII AlwNI ApaI AvaI BanI BanII BbeI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstXI BstYI BtgI BtgZI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglI BmtI Bpu10I BsaAI BsaWI BsiWI BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI