Restriction map for
pFN21AB3085
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanII BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI HphI KpnI KspI MaeI
MfeI MluI MroI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 58 GGCGCC G[GA]^CG[CT]C
AluI 20 AGCT AG^CT
AspHI 171 GAGCAC G[AT]GC[AT]^C
BbeI 58 GGCGCC GGCGC^C
BbsI 325 GAAGAC GAAGAC(2/6)
BcgI 422 GCAATAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 176 CCGGG CC^[GC]GG
BpuAI 325 GAAGAC GAAGAC(2/6)
BsaAI 628 TACGTG [CT]AC^GT[GA]
BsaBI 276 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 58 GGCGCC G[GA]^CG[CT]C
BsiHKAI 171 GAGCAC G[AT]GC[AT]^C
BsmFI 557 GTCCC GGGAC(10/14)
BsrDI 227 CATTGC GCAATG(2/0)
BsrFI 106 GCCGGC [GA]^CCGG[CT]
BstAPI 235 GCAACCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 182 AGATCC [GA]^GATC[CT]
Cfr10I 106 GCCGGC [GA]^CCGG[CT]
Cfr13I 590 GGCCC G^G[ACGT]CC
DdeI 217 CTGAG C^T[ACGT]AG
DraII 589 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 493 CTCTTC CTCTTC(1/4)
EcoO109I 589 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 439 GAATTC G^AATTC
EcoT14I 586 CCAAGG C^C[AT][AT]GG
HaeII 58 GGCGCC [GA]GCGC^[CT]
Hin1I 58 GGCGCC G[GA]^CG[CT]C
KasI 58 GGCGCC G^GCGCC
Ksp632I 493 CTCTTC CTCTTC(1/4)
MaeII 629 ACGT A^CGT
MaeIII 121 GTGAC ^GT[ACGT]AC
MamI 276 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 182 AGATCC [GA]^GATC[CT]
NaeI 106 GCCGGC GCC^GGC
NarI 58 GGCGCC GG^CGCC
NciI 176 CCGGG CC^[GC]GG
NgoMIV 106 GCCGGC G^CCGGC
PflMI 407 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 202 GACTC GAGTC(4/5)
PshAI 456 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 16 CTGCAG CTGCA^G
PvuII 19 CAGCTG CAG^CTG
Sau96I 590 GGCCC G^G[ACGT]CC
SfaNI 260 GCATC GCATC(5/9)
SfcI 16 CTGCAG C^T[GA][CT]AG
SfoI 58 GGCGCC GGC^GCC
StyI 586 CCAAGG C^C[AT][AT]GG
Tsp45I 121 GTGAC ^GT[GC]AC
Van91I 407 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 182 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 9 CGCG CG^CG
369 CGCG
AcsI 439 GAATTC [GA]^AATT[CT]
478 GAATTT
AfaI 138 GTAC GT^AC
474 GTAC
AlwI 183 GATCC GGATC(4/5)
53 GGATC
ApoI 439 GAATTC [GA]^AATT[CT]
478 GAATTT
BalI 2 TGGCCA TGG^CCA
290 TGGCCA
BanI 58 GGCGCC G^G[CT][GA]CC
535 GGTGCC
BmyI 171 GAGCAC G[AGT]GC[ACT]^C
536 GTGCCC
BsgI 605 CTGCAC GTGCAG(16/14)
337 GTGCAG
Bsp1286I 171 GAGCAC G[AGT]GC[ACT]^C
536 GTGCCC
BspMI 603 ACCTGC ACCTGC(4/8)
385 GCAGGT
BstUI 9 CGCG CG^CG
369 CGCG
Cac8I 106 GCCGGC GC[ACGT]^[ACGT]GC
231 GCAAGC
CfoI 59 GCGC GCG^C
368 GCGC
DpnII 54 GATC ^GATC
183 GATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
290 TGGCCA
FauI 10 GCGGG CCCGC(4/6)
617 GCGGG
HapII 107 CCGG C^CGG
176 CCGG
HhaI 59 GCGC GCG^C
368 GCGC
HinP1I 59 GCGC G^CGC
368 GCGC
HpaII 107 CCGG C^CGG
176 CCGG
MboI 54 GATC ^GATC
183 GATC
MluNI 2 TGGCCA TGG^CCA
290 TGGCCA
MscI 2 TGGCCA TGG^CCA
290 TGGCCA
MspA1I 19 CAGCTG C[AC]G^C[GT]G
615 CAGCGG
MspI 107 CCGG C^CGG
176 CCGG
MvnI 9 CGCG CG^CG
369 CGCG
MwoI 235 GCAACCCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
385 GCAGGTCTGGC
NdeII 54 GATC ^GATC
183 GATC
NlaIV 58 GGCGCC GG[ACGT]^[ACGT]CC
535 GGTGCC
RsaI 138 GTAC GT^AC
474 GTAC
Sau3AI 54 GATC ^GATC
183 GATC
TfiI 213 GAATC G^A[AT]TC
428 GAATC
Tsp509I 440 AATT ^AATT
479 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MboII MnlI MvaI NlaIII ScrFI
TseI TspRI