Restriction map for
pFN21AE1419
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BpuAI BsaAI BsaHI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 196 GAATTC [GA]^AATT[CT]
AgeI 777 ACCGGT A^CCGGT
AlwNI 133 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 196 GAATTC [GA]^AATT[CT]
Asp700I 172 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 258 CCTAGG C^CTAGG
BalI 653 TGGCCA TGG^CCA
BamHI 608 GGATCC G^GATCC
BanI 283 GGCACC G^G[CT][GA]CC
BcnI 379 CCCGG CC^[GC]GG
BglI 437 GCCCAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 258 CCTAGG C^CTAGG
BpmI 746 CTCCAG CTGGAG(16/14)
BsaBI 591 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 502 GAGACC GGTCTC(1/5)
BsaWI 777 ACCGGT [AT]^CCGG[AT]
BsmFI 560 GTCCC GGGAC(10/14)
BsmI 172 GAATGC GAATGC(1/-1)
BsrBI 366 CCGCTC CCGCTC(-3/-3)
BsrFI 777 ACCGGT [GA]^CCGG[CT]
BstBI 472 TTCGAA TT^CGAA
BstEII 780 GGTCACC G^GT[ACGT]ACC
BstPI 780 GGTCACC G^GT[ACGT]ACC
BstYI 608 GGATCC [GA]^GATC[CT]
Cfr10I 777 ACCGGT [GA]^CCGG[CT]
DraII 674 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 653 TGGCCA [CT]^GGCC[GA]
EcoO109I 674 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 780 GGTCACC G^GT[ACGT]ACC
EcoRI 196 GAATTC G^AATTC
HgaI 23 GCGTC GACGC(5/10)
HinfI 804 GAGTC G^A[ACGT]TC
MamI 591 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 608 GGATCC [GA]^GATC[CT]
MluNI 653 TGGCCA TGG^CCA
MscI 653 TGGCCA TGG^CCA
MslI 810 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 47 CCGCTG C[AC]G^C[GT]G
NciI 379 CCCGG CC^[GC]GG
NspI 73 GCATGC [GA]CATG^[CT]
NspV 472 TTCGAA TT^CGAA
PinAI 777 ACCGGT A^CCGGT
PleI 804 GAGTC GAGTC(4/5)
PshAI 45 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 16 CTGCAG CTGCA^G
SexAI 512 ACCTGGT A^CC[AT]GGT
SfuI 472 TTCGAA TT^CGAA
SphI 73 GCATGC GCATG^C
StuI 341 AGGCCT AGG^CCT
TaqI 473 TCGA T^CGA
TthHB8I 473 TCGA T^CGA
XhoII 608 GGATCC [GA]^GATC[CT]
XmnI 172 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 90 GTAC GT^AC
332 GTAC
AluI 352 AGCT AG^CT
496 AGCT
AvaII 270 GGTCC G^G[AT]CC
559 GGTCC
BanII 430 GAGCCC G[GA]GC[CT]^C
578 GAGCCC
BbvI 35 GCTGC GCAGC(8/12)
742 GCTGC
BfaI 202 CTAG C^TAG
259 CTAG
EcoT14I 258 CCTAGG C^C[AT][AT]GG
487 CCTTGG
Fnu4HI 35 GCTGC GC^[ACGT]GC
742 GCTGC
HapII 380 CCGG C^CGG
778 CCGG
HpaII 380 CCGG C^CGG
778 CCGG
ItaI 35 GCTGC GC^[ACGT]GC
742 GCTGC
MaeI 202 CTAG C^TAG
259 CTAG
MspI 380 CCGG C^CGG
778 CCGG
RsaI 90 GTAC GT^AC
332 GTAC
SfaNI 770 GATGC GCATC(5/9)
809 GCATC
SfcI 16 CTGCAG C^T[GA][CT]AG
404 CTACAG
StyI 258 CCTAGG C^C[AT][AT]GG
487 CCTTGG
TseI 35 GCTGC G^C[AT]GC
742 GCTGC
Tsp509I 197 AATT ^AATT
709 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AlwI BmyI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII EcoRII FokI
HaeIII HphI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI Tsp45I
TspRI