(Circular) (Six-base) MAPSORT of: pFN21AE1419.seq Check: 5932 from: 1 to: 5536 pFN21AE1419 5536 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2879 1937 Size: 942 4594 Acc65I G'GTAC_C Cuts at: 2857 2857 Size: 5536 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3599 4983 5251 1632 Size: 1967 1384 268 1917 Fragments arranged by size: 1967 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 4435 5412 1203 Size: 3232 977 1327 Fragments arranged by size: 3232 1327 977 AgeI A'CCGG_T Cuts at: 2782 4367 2782 Size: 1585 3951 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2837 1184 Size: 1653 3883 AlwNI CAG_nnn'CTG Cuts at: 2143 3279 4851 2143 Size: 1136 1572 2828 Fragments arranged by size: 2828 1572 1136 ApaLI G'TGCA_C Cuts at: 3593 4749 3593 Size: 1156 4380 ApoI r'AATT_y Cuts at: 1493 2201 2845 3071 3223 5332 1493 Size: 708 644 226 152 2109 1697 Fragments arranged by size: 2109 1697 708 644 226 152 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2861 1870 Size: 73 918 4545 Fragments arranged by size: 4545 918 73 AvrII C'CTAG_G Cuts at: 2263 2263 Size: 5536 BamHI G'GATC_C Cuts at: 2613 2866 3233 2613 Size: 253 367 4916 Fragments arranged by size: 4916 367 253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2288 2857 4312 618 Size: 325 217 627 501 569 1455 1842 Fragments arranged by size: 1842 1455 627 569 501 325 217 BanII G_rGCy'C Cuts at: 1391 2439 2587 2855 1391 Size: 1048 148 268 4072 Fragments arranged by size: 4072 1048 268 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3727 3761 1903 Size: 34 1790 34 3678 Fragments arranged by size: 3678 1790 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4638 3428 Size: 1210 4326 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2448 4147 136 Size: 107 122 71 631 605 776 1699 1525 Fragments arranged by size: 1699 1525 776 631 605 122 107 71 BglII A'GATC_T Cuts at: 5531 5531 Size: 5536 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 2291 3597 4753 1790 Size: 501 1306 1156 2573 Fragments arranged by size: 2573 1306 1156 501 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2438 3267 4225 Size: 416 53 83 399 24 1021 829 958 Cuts at: 4225 442 Size: 1753 Fragments arranged by size: 1753 1021 958 829 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2736 4196 1239 Size: 1497 1460 2579 Fragments arranged by size: 2579 1497 1460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3661 4526 4824 3661 Size: 865 298 4373 Fragments arranged by size: 4373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2501 4199 882 Size: 617 1002 1698 2219 Fragments arranged by size: 2219 1698 1002 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 493 Size: 711 2123 2702 Fragments arranged by size: 2702 2123 711 BsaBI GATnn'nnATC Cuts at: 2600 5530 2600 Size: 2930 2606 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3343 3725 4360 275 Size: 53 83 186 936 1810 382 635 1451 Fragments arranged by size: 1810 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2782 3969 4367 4641 4788 5199 2782 Size: 1187 398 274 147 411 3119 Fragments arranged by size: 3119 1187 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2734 2764 1178 Size: 30 165 30 1331 30 3950 Fragments arranged by size: 3950 1331 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5536 BseYI C'CCAG_C Cuts at: 4739 5235 4739 Size: 496 5040 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2140 2296 2351 2777 2843 1163 Size: 573 404 156 55 426 66 3856 Fragments arranged by size: 3856 573 426 404 156 66 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2986 3747 4775 1540 Size: 275 186 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3597 3682 4753 3597 Size: 85 1071 4380 Fragments arranged by size: 4380 1071 85 BsmI GAATG_Cn' Cuts at: 2183 3047 3140 2183 Size: 864 93 4579 Fragments arranged by size: 4579 864 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2291 2439 2587 2855 3597 3682 4753 Size: 399 501 148 148 268 742 85 1071 Cuts at: 4753 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 501 399 268 148 148 85 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 3425 5155 3425 Size: 1730 3806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2891 844 Size: 2047 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2373 3423 1394 Size: 429 550 1050 3507 Fragments arranged by size: 3507 1050 550 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4031 4205 65 Size: 1183 2783 174 1396 Fragments arranged by size: 2783 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2782 4180 4367 1700 Size: 110 972 1398 187 2869 Fragments arranged by size: 2869 1398 972 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5536 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5536 BssSI C'ACGA_G Cuts at: 3596 4608 3596 Size: 1012 4524 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5536 BstBI TT'CG_AA Cuts at: 2478 4340 2478 Size: 1862 3674 BstEII G'GTnAC_C Cuts at: 1184 2785 1184 Size: 1601 3935 BstXI CCAn_nnnn'nTGG Cuts at: 2518 2632 2798 2518 Size: 114 166 5256 Fragments arranged by size: 5256 166 114 BstYI r'GATC_y Cuts at: 1972 2613 2866 3233 3618 3635 5087 5173 5185 Size: 641 253 367 385 17 1452 86 12 Cuts at: 5185 5531 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 641 385 367 346 253 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4045 Fragments arranged by size: 4045 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 2030 2073 2230 2742 3052 3846 3866 2030 Size: 43 157 512 310 794 20 3700 Fragments arranged by size: 3700 794 512 310 157 43 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5501 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4543 809 Size: 507 3227 1802 Fragments arranged by size: 3227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2658 2983 3872 Size: 54 1425 50 275 18 828 325 889 Cuts at: 3872 5501 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 828 325 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2983 1537 Size: 275 1171 4090 Fragments arranged by size: 4090 1171 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 1353 Size: 2113 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4123 4497 4643 387 Size: 1064 138 2534 374 146 1280 Fragments arranged by size: 2534 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2736 3599 4196 4983 5251 1239 Size: 393 1104 863 597 787 268 1524 Fragments arranged by size: 1524 1104 863 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2680 2680 Size: 5536 EcoRI G'AATT_C Cuts at: 2201 2845 3223 2201 Size: 644 378 4514 Fragments arranged by size: 4514 644 378 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 4683 1678 Size: 3005 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373 Size: 32 135 32 2605 32 42 32 2626 Fragments arranged by size: 2626 2605 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KpnI G_GTAC'C Cuts at: 2861 2861 Size: 5536 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2763 4650 4834 1305 Size: 1458 1887 184 2007 Fragments arranged by size: 2007 1887 1458 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2660 10 Size: 54 1425 343 828 2886 Fragments arranged by size: 2886 1425 828 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2819 3494 3853 4012 518 Size: 648 1653 675 359 159 2042 Fragments arranged by size: 2042 1653 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2952 3629 4777 5022 1606 Size: 448 898 677 1148 245 2120 Fragments arranged by size: 2120 1148 898 677 448 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4045 Fragments arranged by size: 4045 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2983 2983 Size: 5536 NspI r_CATG'y Cuts at: 1132 1229 2082 2894 4439 1132 Size: 97 853 812 1545 2229 Fragments arranged by size: 2229 1545 853 812 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5536 PciI A'CATG_T Cuts at: 4435 4435 Size: 5536 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2054 1613 Size: 441 5095 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PstI C_TGCA'G Cuts at: 830 2025 2888 830 Size: 1195 863 3478 Fragments arranged by size: 3478 1195 863 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SbfI CC_TGCA'GG Cuts at: 2888 2888 Size: 5536 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SexAI A'CCwGG_T Cuts at: 2517 2517 Size: 5536 SfcI C'TryA_G Cuts at: 826 1045 2021 2409 2884 4019 4700 4891 826 Size: 219 976 388 475 1135 681 191 1471 Fragments arranged by size: 1471 1135 976 681 475 388 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2863 1872 Size: 991 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3640 4541 4803 820 Size: 197 926 1697 901 262 1553 Fragments arranged by size: 1697 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2082 2894 1132 Size: 950 812 3774 Fragments arranged by size: 3774 950 812 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 2348 1517 Size: 831 4705 StyI C'CwwG_G Cuts at: 513 1065 2004 2263 2492 2975 513 Size: 552 939 259 229 483 3074 Fragments arranged by size: 3074 939 552 483 259 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2086 2804 3563 3580 3733 3918 4363 Size: 1255 506 718 759 17 153 185 445 Cuts at: 4363 325 Size: 1498 Fragments arranged by size: 1498 1255 759 718 506 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3782 5494 96 Size: 274 80 33 51 1443 1805 1712 138 Fragments arranged by size: 1805 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2488 2520 2734 2766 5496 5528 2488 Size: 32 214 32 2730 32 2496 Fragments arranged by size: 2730 2496 214 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2861 1870 Size: 991 4545 XmnI GAAnn'nnTTC Cuts at: 2181 3665 2181 Size: 1484 4052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI